Restriction map for
pF1KSDA1184
(Linear) (Six-base) MAPSORT of: KIAA1184.seq Check: 7407 from: 1 to: 1155
KIAA1184.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 892 1155
Size: 892 263
AccI GT'mk_AC
Cuts at: 0 704 1155
Size: 704 451
Acc65I G'GTAC_C
Cuts at: 0 266 1155
Size: 266 889
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 428 640 1153 1155
Size: 49 379 212 513 2
Fragments arranged by size:
513 379 212 49 2
AfeI AGC'GCT
Cuts at: 0 1009 1155
Size: 1009 146
AlwNI CAG_nnn'CTG
Cuts at: 0 288 966 1113 1155
Size: 288 678 147 42
Fragments arranged by size:
678 288 147 42
ApaI G_GGCC'C
Cuts at: 0 120 669 1078 1107 1155
Size: 120 549 409 29 48
Fragments arranged by size:
549 409 120 48 29
AvaI C'yCGr_G
Cuts at: 0 113 262 452 916 929 1100 1155
Size: 113 149 190 464 13 171 55
Fragments arranged by size:
464 190 171 149 113 55 13
BanI G'GyrC_C
Cuts at: 0 266 560 1155
Size: 266 294 595
Fragments arranged by size:
595 294 266
BanII G_rGCy'C
Cuts at: 0 120 300 589 669 725 820 917 1078
Size: 120 180 289 80 56 95 97 161
Cuts at: 1078 1107 1124 1155
Size: 29 17 31
Fragments arranged by size:
289 180 161 120 97 95 80 56
31 29 17
BbvCI CC'TCA_GC
Cuts at: 0 550 1155
Size: 550 605
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 243 277 1155
Size: 243 34 878
Fragments arranged by size:
878 243 34
BglI GCCn_nnn'nGGC
Cuts at: 0 424 1155
Size: 424 731
BlpI GC'TnA_GC
Cuts at: 0 814 1155
Size: 814 341
Bme1580I G_kGCm'C
Cuts at: 0 120 669 982 988 1078 1107 1155
Size: 120 549 313 6 90 29 48
Fragments arranged by size:
549 313 120 90 48 29 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1091 1155
Size: 531 560 64
Fragments arranged by size:
560 531 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1026 1155
Size: 1026 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1155
Size: 988 167
Bpu10I CC'TnA_GC
Cuts at: 0 550 1155
Size: 550 605
BsaI GGTCTCn'nnnn_
Cuts at: 0 799 1155
Size: 799 356
BsaAI yAC'GTr
Cuts at: 0 983 1155
Size: 983 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 344 374 1155
Size: 344 30 781
Fragments arranged by size:
781 344 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 898 1018 1155
Size: 898 120 137
Fragments arranged by size:
898 137 120
BseYI C'CCAG_C
Cuts at: 0 332 419 834 1106 1155
Size: 332 87 415 272 49
Fragments arranged by size:
415 332 272 87 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 973 1155
Size: 481 492 182
Fragments arranged by size:
492 481 182
BsiHKAI G_wGCw'C
Cuts at: 0 820 1124 1155
Size: 820 304 31
Fragments arranged by size:
820 304 31
Bsp1286I G_dGCh'C
Cuts at: 0 120 300 589 669 725 820 917 982
Size: 120 180 289 80 56 95 97 65
Cuts at: 982 988 1078 1107 1124 1155
Size: 6 90 29 17 31
Fragments arranged by size:
289 180 120 97 95 90 80 65
56 31 29 17 6
BspHI T'CATG_A
Cuts at: 0 870 1155
Size: 870 285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 767 892 1155
Size: 767 125 263
Fragments arranged by size:
767 263 125
BsrDI GCAATG_nn'
Cuts at: 0 804 1155
Size: 804 351
BsrFI r'CCGG_y
Cuts at: 0 554 1127 1155
Size: 554 573 28
Fragments arranged by size:
573 554 28
BsrGI T'GTAC_A
Cuts at: 0 245 1155
Size: 245 910
BstAPI GCAn_nnn'nTGC
Cuts at: 0 951 1155
Size: 951 204
BstEII G'GTnAC_C
Cuts at: 0 486 1155
Size: 486 669
BstYI r'GATC_y
Cuts at: 0 659 1155
Size: 659 496
Bsu36I CC'TnA_GG
Cuts at: 0 590 1155
Size: 590 565
BtsI GCAGTG_nn'
Cuts at: 0 952 1035 1155
Size: 952 83 120
Fragments arranged by size:
952 120 83
EaeI y'GGCC_r
Cuts at: 0 681 1155
Size: 681 474
EarI CTCTTCn'nnn_
Cuts at: 0 277 349 751 769 1112 1155
Size: 277 72 402 18 343 43
Fragments arranged by size:
402 343 277 72 43 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 798 1114 1155
Size: 798 316 41
Fragments arranged by size:
798 316 41
EcoICRI GAG'CTC
Cuts at: 0 818 1122 1155
Size: 818 304 33
Fragments arranged by size:
818 304 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 428 640 988 1153 1155
Size: 49 379 212 348 165 2
Fragments arranged by size:
379 348 212 165 49 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1000 1155
Size: 1000 155
EcoO109I rG'GnC_Cy
Cuts at: 0 110 117 666 733 756 1075 1155
Size: 110 7 549 67 23 319 80
Fragments arranged by size:
549 319 110 80 67 23 7
FspI TGC'GCA
Cuts at: 0 292 1155
Size: 292 863
HaeII r_GCGC'y
Cuts at: 0 28 1011 1048 1155
Size: 28 983 37 107
Fragments arranged by size:
983 107 37 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1155
Size: 1081 32 42
Fragments arranged by size:
1081 42 32
KpnI G_GTAC'C
Cuts at: 0 270 1155
Size: 270 885
MscI TGG'CCA
Cuts at: 0 683 1155
Size: 683 472
MslI CAynn'nnrTG
Cuts at: 0 632 875 1155
Size: 632 243 280
Fragments arranged by size:
632 280 243
MspA1I CmG'CkG
Cuts at: 0 288 423 957 963 1155
Size: 288 135 534 6 192
Fragments arranged by size:
534 288 192 135 6
NaeI GCC'GGC
Cuts at: 0 556 1129 1155
Size: 556 573 26
Fragments arranged by size:
573 556 26
NgoMIV G'CCGG_C
Cuts at: 0 554 1127 1155
Size: 554 573 28
Fragments arranged by size:
573 554 28
PasI CC'CwG_GG
Cuts at: 0 996 1155
Size: 996 159
PflMI CCAn_nnn'nTGG
Cuts at: 0 1041 1113 1155
Size: 1041 72 42
Fragments arranged by size:
1041 72 42
PfoI T'CCnGG_A
Cuts at: 0 1112 1155
Size: 1112 43
PmlI CAC'GTG
Cuts at: 0 983 1155
Size: 983 172
PpuMI rG'GwC_Cy
Cuts at: 0 110 733 756 1155
Size: 110 623 23 399
Fragments arranged by size:
623 399 110 23
PspOMI G'GGCC_C
Cuts at: 0 116 665 1074 1103 1155
Size: 116 549 409 29 52
Fragments arranged by size:
549 409 116 52 29
PvuII CAG'CTG
Cuts at: 0 288 423 1155
Size: 288 135 732
Fragments arranged by size:
732 288 135
SacI G_AGCT'C
Cuts at: 0 820 1124 1155
Size: 820 304 31
Fragments arranged by size:
820 304 31
SanDI GG'GwC_CC
Cuts at: 0 733 1155
Size: 733 422
SapI GCTCTTCn'nnn_
Cuts at: 0 769 1112 1155
Size: 769 343 43
Fragments arranged by size:
769 343 43
SfcI C'TryA_G
Cuts at: 0 390 1048 1155
Size: 390 658 107
Fragments arranged by size:
658 390 107
SmaI CCC'GGG
Cuts at: 0 115 931 1102 1155
Size: 115 816 171 53
Fragments arranged by size:
816 171 115 53
StyI C'CwwG_G
Cuts at: 0 491 1155
Size: 491 664
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 427 628 939 1155
Size: 427 201 311 216
Fragments arranged by size:
427 311 216 201
TatI w'GTAC_w
Cuts at: 0 245 508 1155
Size: 245 263 647
Fragments arranged by size:
647 263 245
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 344 376 1155
Size: 344 32 779
Fragments arranged by size:
779 344 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 1155
Size: 219 936
XmaI C'CCGG_G
Cuts at: 0 113 929 1100 1155
Size: 113 816 171 55
Fragments arranged by size:
816 171 113 55
XmnI GAAnn'nnTTC
Cuts at: 0 185 1155
Size: 185 970
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AlwNI ApaI AvaI
BanI BanII BbvCI BcgI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BstAPI BstEII BstYI Bsu36I BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI
MscI MslI MspA1I NaeI NgoMIV PasI PflMI PfoI
PmlI PpuMI PspOMI PvuII SacI SanDI SapI SfcI
SmaI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BciVI BclI BfrBI BglII BmgBI
BmtI BpuEI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BssHII BssSI BstBI BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI MfeI MluI MmeI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI ZraI