Restriction map for
pF1KA1170
(Linear) (Six-base) MAPSORT of: KIAA1170.seq Check: 4255 from: 1 to: 2274
KIAA1170.orf
With 182 enzymes: SgfI *
February 20, 2009 16:03 ..
Acc65I G'GTAC_C
Cuts at: 0 1488 2274
Size: 1488 786
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 932 1849 2274
Size: 394 538 917 425
Fragments arranged by size:
917 538 425 394
AflII C'TTAA_G
Cuts at: 0 1561 2274
Size: 1561 713
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1969 2001 2274
Size: 1969 32 273
Fragments arranged by size:
1969 273 32
ApaI G_GGCC'C
Cuts at: 0 34 1263 2091 2274
Size: 34 1229 828 183
Fragments arranged by size:
1229 828 183 34
ApoI r'AATT_y
Cuts at: 0 226 2274
Size: 226 2048
AvaI C'yCGr_G
Cuts at: 0 383 680 2274
Size: 383 297 1594
Fragments arranged by size:
1594 383 297
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1340 1373 2274
Size: 1340 33 901
Fragments arranged by size:
1340 901 33
BanI G'GyrC_C
Cuts at: 0 81 993 1191 1212 1488 2070 2162 2274
Size: 81 912 198 21 276 582 92 112
Fragments arranged by size:
912 582 276 198 112 92 81 21
BanII G_rGCy'C
Cuts at: 0 34 133 596 1106 1263 2091 2274
Size: 34 99 463 510 157 828 183
Fragments arranged by size:
828 510 463 183 157 99 34
BbeI G_GCGC'C
Cuts at: 0 85 2166 2274
Size: 85 2081 108
Fragments arranged by size:
2081 108 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 920 1396 2252 2274
Size: 920 476 856 22
Fragments arranged by size:
920 856 476 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 931 2274
Size: 931 1343
BfrBI ATG'CAT
Cuts at: 0 717 2274
Size: 717 1557
BglI GCCn_nnn'nGGC
Cuts at: 0 316 1025 2274
Size: 316 709 1249
Fragments arranged by size:
1249 709 316
BlpI GC'TnA_GC
Cuts at: 0 1226 2274
Size: 1226 1048
Bme1580I G_kGCm'C
Cuts at: 0 34 555 1263 1465 2091 2274
Size: 34 521 708 202 626 183
Fragments arranged by size:
708 626 521 202 183 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 2247 2274
Size: 2247 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 313 526 664 1474 1645 1970 1992
Size: 172 141 213 138 810 171 325 22
Cuts at: 1992 2274
Size: 282
Fragments arranged by size:
810 325 282 213 172 171 141 138
22
Bpu10I CC'TnA_GC
Cuts at: 0 595 2274
Size: 595 1679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 2077 2274
Size: 254 1823 197
Fragments arranged by size:
1823 254 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 679 2274
Size: 679 1595
BsaBI GATnn'nnATC
Cuts at: 0 1648 2274
Size: 1648 626
BsaHI Gr'CG_yC
Cuts at: 0 82 1498 2163 2274
Size: 82 1416 665 111
Fragments arranged by size:
1416 665 111 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 2274
Size: 145 30 2099
Fragments arranged by size:
2099 145 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 739 773 1033 1114 1129 1138 1462 1654
Size: 739 34 260 81 15 9 324 192
Cuts at: 1654 1732 2274
Size: 78 542
Fragments arranged by size:
739 542 324 260 192 81 78 34
15 9
BseYI C'CCAG_C
Cuts at: 0 1674 2274
Size: 1674 600
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1450 2274
Size: 295 1155 824
Fragments arranged by size:
1155 824 295
BsiHKAI G_wGCw'C
Cuts at: 0 596 1292 2274
Size: 596 696 982
Fragments arranged by size:
982 696 596
BsmI GAATG_Cn'
Cuts at: 0 1529 2274
Size: 1529 745
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1489 2274
Size: 1489 785
Bsp1286I G_dGCh'C
Cuts at: 0 34 133 555 596 1106 1263 1292 1465
Size: 34 99 422 41 510 157 29 173
Cuts at: 1465 2091 2274
Size: 626 183
Fragments arranged by size:
626 510 422 183 173 157 99 41
34 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1178 2274
Size: 1178 1096
BsrBI CCG'CTC
Cuts at: 0 1230 2274
Size: 1230 1044
BsrDI GCAATG_nn'
Cuts at: 0 54 2274
Size: 54 2220
BsrFI r'CCGG_y
Cuts at: 0 341 2274
Size: 341 1933
BsrGI T'GTAC_A
Cuts at: 0 1529 2274
Size: 1529 745
BstEII G'GTnAC_C
Cuts at: 0 756 2274
Size: 756 1518
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 2052 2157 2274
Size: 504 1548 105 117
Fragments arranged by size:
1548 504 117 105
BstYI r'GATC_y
Cuts at: 0 1083 2274
Size: 1083 1191
Bsu36I CC'TnA_GG
Cuts at: 0 1637 2002 2274
Size: 1637 365 272
Fragments arranged by size:
1637 365 272
BtgI C'CryG_G
Cuts at: 0 752 1023 2274
Size: 752 271 1251
Fragments arranged by size:
1251 752 271
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1404 2274
Size: 1404 870
BtsI GCAGTG_nn'
Cuts at: 0 555 777 1862 2274
Size: 555 222 1085 412
Fragments arranged by size:
1085 555 412 222
ClaI AT'CG_AT
Cuts at: 0 1682 2274
Size: 1682 592
DraI TTT'AAA
Cuts at: 0 2064 2274
Size: 2064 210
EarI CTCTTCn'nnn_
Cuts at: 0 751 928 1093 1115 1439 1475 2274
Size: 751 177 165 22 324 36 799
Fragments arranged by size:
799 751 324 177 165 36 22
EcoICRI GAG'CTC
Cuts at: 0 594 2274
Size: 594 1680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 313 394 526 664 932 1474 1645
Size: 172 141 81 132 138 268 542 171
Cuts at: 1645 1849 1970 1992 2274
Size: 204 121 22 282
Fragments arranged by size:
542 282 268 204 172 171 141 138
132 121 81 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1336 1516 1636 2274
Size: 1336 180 120 638
Fragments arranged by size:
1336 638 180 120
EcoO109I rG'GnC_Cy
Cuts at: 0 6 30 488 804 1182 1248 1260 2088
Size: 6 24 458 316 378 66 12 828
Cuts at: 2088 2274
Size: 186
Fragments arranged by size:
828 458 378 316 186 66 24 12
6
EcoRI G'AATT_C
Cuts at: 0 226 2274
Size: 226 2048
HaeII r_GCGC'y
Cuts at: 0 85 2166 2274
Size: 85 2081 108
Fragments arranged by size:
2081 108 85
HindIII A'AGCT_T
Cuts at: 0 705 2274
Size: 705 1569
KasI G'GCGC_C
Cuts at: 0 81 2162 2274
Size: 81 2081 112
Fragments arranged by size:
2081 112 81
KpnI G_GTAC'C
Cuts at: 0 1492 2274
Size: 1492 782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 1338 2274
Size: 278 1060 936
Fragments arranged by size:
1060 936 278
MslI CAynn'nnrTG
Cuts at: 0 1382 1684 2274
Size: 1382 302 590
Fragments arranged by size:
1382 590 302
MspA1I CmG'CkG
Cuts at: 0 114 568 990 2274
Size: 114 454 422 1284
Fragments arranged by size:
1284 454 422 114
NarI GG'CG_CC
Cuts at: 0 82 2163 2274
Size: 82 2081 111
Fragments arranged by size:
2081 111 82
NcoI C'CATG_G
Cuts at: 0 752 2274
Size: 752 1522
NsiI A_TGCA'T
Cuts at: 0 719 2274
Size: 719 1555
NspI r_CATG'y
Cuts at: 0 184 942 2274
Size: 184 758 1332
Fragments arranged by size:
1332 758 184
PasI CC'CwG_GG
Cuts at: 0 1256 1332 2274
Size: 1256 76 942
Fragments arranged by size:
1256 942 76
PfoI T'CCnGG_A
Cuts at: 0 503 1886 1910 2038 2274
Size: 503 1383 24 128 236
Fragments arranged by size:
1383 503 236 128 24
PpuMI rG'GwC_Cy
Cuts at: 0 6 488 804 1182 2274
Size: 6 482 316 378 1092
Fragments arranged by size:
1092 482 378 316 6
PspOMI G'GGCC_C
Cuts at: 0 30 1259 2087 2274
Size: 30 1229 828 187
Fragments arranged by size:
1229 828 187 30
PstI C_TGCA'G
Cuts at: 0 772 863 1581 2274
Size: 772 91 718 693
Fragments arranged by size:
772 718 693 91
PvuII CAG'CTG
Cuts at: 0 568 990 2274
Size: 568 422 1284
Fragments arranged by size:
1284 568 422
SacI G_AGCT'C
Cuts at: 0 596 2274
Size: 596 1678
SapI GCTCTTCn'nnn_
Cuts at: 0 928 2274
Size: 928 1346
SfcI C'TryA_G
Cuts at: 0 543 768 859 1577 2274
Size: 543 225 91 718 697
Fragments arranged by size:
718 697 543 225 91
SfoI GGC'GCC
Cuts at: 0 83 2164 2274
Size: 83 2081 110
Fragments arranged by size:
2081 110 83
SmaI CCC'GGG
Cuts at: 0 385 682 2274
Size: 385 297 1592
Fragments arranged by size:
1592 385 297
SmlI C'TyrA_G
Cuts at: 0 269 1561 2092 2274
Size: 269 1292 531 182
Fragments arranged by size:
1292 531 269 182
SphI G_CATG'C
Cuts at: 0 184 942 2274
Size: 184 758 1332
Fragments arranged by size:
1332 758 184
SrfI GCCC'GGGC
Cuts at: 0 385 2274
Size: 385 1889
SspI AAT'ATT
Cuts at: 0 636 2274
Size: 636 1638
StuI AGG'CCT
Cuts at: 0 319 541 2274
Size: 319 222 1733
Fragments arranged by size:
1733 319 222
StyI C'CwwG_G
Cuts at: 0 86 405 752 1151 1244 2274
Size: 86 319 347 399 93 1030
Fragments arranged by size:
1030 399 347 319 93 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 656 2274
Size: 656 1618
TatI w'GTAC_w
Cuts at: 0 873 1529 2274
Size: 873 656 745
Fragments arranged by size:
873 745 656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 412 2274
Size: 412 1862
XmaI C'CCGG_G
Cuts at: 0 383 680 2274
Size: 383 297 1594
Fragments arranged by size:
1594 383 297
XmnI GAAnn'nnTTC
Cuts at: 0 635 1315 1834 2274
Size: 635 680 519 440
Fragments arranged by size:
680 635 519 440
Enzymes that do cut:
Acc65I AcuI AflII AloI ApaI ApoI AvaI BaeI
BanI BanII BbeI BbsI BciVI BfrBI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII HindIII KasI KpnI
MmeI MslI MspA1I NarI NcoI NsiI NspI PasI
PfoI PpuMI PspOMI PstI PvuII SacI SapI SfcI
SfoI SmaI SmlI SphI SrfI SspI StuI StyI
TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI AscI AseI AsiSI AvrII
BamHI BbvCI BcgI BclI BglII BmgBI BmtI BplI
BsaAI BsaWI BsiEI BsiWI BspEI BspHI BssHII BssSI
BstAPI BstBI BstZ17I CspCI DraIII DrdI EaeI EagI
EciI EcoRV FalI FseI FspI FspAI Hin4I HincII
HpaI MfeI MluI MscI NaeI NdeI NgoMIV NheI
NotI NruI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SwaI TstI Tth111I XbaI XhoI ZraI