Restriction map for
pF1KA1153
(Linear) (Six-base) MAPSORT of: KIAA1153.seq Check: 6561 from: 1 to: 1200
KIAA1153.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 219 1200
Size: 219 981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1147 1200
Size: 812 335 53
Fragments arranged by size:
812 335 53
AflIII A'CryG_T
Cuts at: 0 622 1200
Size: 622 578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1128 1200
Size: 1128 72
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 692 724 1200
Size: 692 32 476
Fragments arranged by size:
692 476 32
ApaI G_GGCC'C
Cuts at: 0 28 1200
Size: 28 1172
ApoI r'AATT_y
Cuts at: 0 418 766 1200
Size: 418 348 434
Fragments arranged by size:
434 418 348
BanII G_rGCy'C
Cuts at: 0 10 28 1158 1200
Size: 10 18 1130 42
Fragments arranged by size:
1130 42 18 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 784 914 953 1200
Size: 784 130 39 247
Fragments arranged by size:
784 247 130 39
Bme1580I G_kGCm'C
Cuts at: 0 28 274 1200
Size: 28 246 926
Fragments arranged by size:
926 246 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 895 1200
Size: 716 179 305
Fragments arranged by size:
716 305 179
Bpu10I CC'TnA_GC
Cuts at: 0 9 1200
Size: 9 1191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 959 1200
Size: 305 654 241
Fragments arranged by size:
654 305 241
BsaI GGTCTCn'nnnn_
Cuts at: 0 41 1200
Size: 41 1159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 692 722 1200
Size: 692 30 478
Fragments arranged by size:
692 478 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 713 1056 1130 1200
Size: 713 343 74 70
Fragments arranged by size:
713 343 74 70
BseYI C'CCAG_C
Cuts at: 0 639 1200
Size: 639 561
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 1200
Size: 647 553
BsmI GAATG_Cn'
Cuts at: 0 1176 1200
Size: 1176 24
Bsp1286I G_dGCh'C
Cuts at: 0 10 28 274 1158 1200
Size: 10 18 246 884 42
Fragments arranged by size:
884 246 42 18 10
BsrBI CCG'CTC
Cuts at: 0 1084 1200
Size: 1084 116
BsrDI GCAATG_nn'
Cuts at: 0 653 1200
Size: 653 547
BstBI TT'CG_AA
Cuts at: 0 140 1200
Size: 140 1060
BstXI CCAn_nnnn'nTGG
Cuts at: 0 184 1200
Size: 184 1016
BstYI r'GATC_y
Cuts at: 0 978 1200
Size: 978 222
DraI TTT'AAA
Cuts at: 0 102 1200
Size: 102 1098
EaeI y'GGCC_r
Cuts at: 0 174 1200
Size: 174 1026
EarI CTCTTCn'nnn_
Cuts at: 0 229 236 1046 1200
Size: 229 7 810 154
Fragments arranged by size:
810 229 154 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 812 895 1147 1200
Size: 716 96 83 252 53
Fragments arranged by size:
716 252 96 83 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 698 1200
Size: 698 502
EcoO109I rG'GnC_Cy
Cuts at: 0 703 924 994 1200
Size: 703 221 70 206
Fragments arranged by size:
703 221 206 70
EcoRI G'AATT_C
Cuts at: 0 766 1200
Size: 766 434
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 1200
Size: 289 32 879
Fragments arranged by size:
879 289 32
HincII GTy'rAC
Cuts at: 0 575 1200
Size: 575 625
HindIII A'AGCT_T
Cuts at: 0 335 1200
Size: 335 865
MscI TGG'CCA
Cuts at: 0 176 1200
Size: 176 1024
MslI CAynn'nnrTG
Cuts at: 0 182 1200
Size: 182 1018
MspA1I CmG'CkG
Cuts at: 0 120 1200
Size: 120 1080
NspI r_CATG'y
Cuts at: 0 728 1152 1200
Size: 728 424 48
Fragments arranged by size:
728 424 48
PfoI T'CCnGG_A
Cuts at: 0 42 1200
Size: 42 1158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 692 724 1200
Size: 692 32 476
Fragments arranged by size:
692 476 32
PpuMI rG'GwC_Cy
Cuts at: 0 703 1200
Size: 703 497
PspOMI G'GGCC_C
Cuts at: 0 24 1200
Size: 24 1176
PstI C_TGCA'G
Cuts at: 0 255 1200
Size: 255 945
SapI GCTCTTCn'nnn_
Cuts at: 0 236 1046 1200
Size: 236 810 154
Fragments arranged by size:
810 236 154
SfcI C'TryA_G
Cuts at: 0 220 251 1200
Size: 220 31 949
Fragments arranged by size:
949 220 31
SmlI C'TyrA_G
Cuts at: 0 320 974 1200
Size: 320 654 226
Fragments arranged by size:
654 320 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 631 956 1100 1200
Size: 631 325 144 100
Fragments arranged by size:
631 325 144 100
TatI w'GTAC_w
Cuts at: 0 108 394 1200
Size: 108 286 806
Fragments arranged by size:
806 286 108
XmnI GAAnn'nnTTC
Cuts at: 0 371 807 1200
Size: 371 436 393
Fragments arranged by size:
436 393 371
Enzymes that do cut:
AccI AcuI AflIII AlfI AloI ApaI ApoI BanII
BbsI Bme1580I BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BsrBI BsrDI BstBI BstXI
BstYI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
Hin4I HincII HindIII MscI MslI MspA1I NspI PfoI
PpiI PpuMI PspOMI PstI SapI SfcI SmlI TaqII
TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI