Restriction map for
pF1KA1150
(Linear) (Six-base) MAPSORT of: KIAA1150.seq Check: 7440 from: 1 to: 1779
KIAA1150.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AflIII A'CryG_T
Cuts at: 0 773 789 1779
Size: 773 16 990
Fragments arranged by size:
990 773 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1779
Size: 979 32 768
Fragments arranged by size:
979 768 32
ApaI G_GGCC'C
Cuts at: 0 693 835 1779
Size: 693 142 944
Fragments arranged by size:
944 693 142
ApoI r'AATT_y
Cuts at: 0 711 1779
Size: 711 1068
BanI G'GyrC_C
Cuts at: 0 174 1605 1779
Size: 174 1431 174
Fragments arranged by size:
1431 174 174
BanII G_rGCy'C
Cuts at: 0 693 835 1779
Size: 693 142 944
Fragments arranged by size:
944 693 142
BbsI GAAGACnn'nnnn_
Cuts at: 0 601 1779
Size: 601 1178
BbvCI CC'TCA_GC
Cuts at: 0 505 1779
Size: 505 1274
BfrBI ATG'CAT
Cuts at: 0 1390 1779
Size: 1390 389
BglI GCCn_nnn'nGGC
Cuts at: 0 1060 1779
Size: 1060 719
Bme1580I G_kGCm'C
Cuts at: 0 693 835 1263 1779
Size: 693 142 428 516
Fragments arranged by size:
693 516 428 142
BmrI ACTGGGnnnn_n'
Cuts at: 0 1257 1605 1691 1779
Size: 1257 348 86 88
Fragments arranged by size:
1257 348 88 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1114 1454 1537 1779
Size: 584 530 340 83 242
Fragments arranged by size:
584 530 340 242 83
Bpu10I CC'TnA_GC
Cuts at: 0 505 839 1779
Size: 505 334 940
Fragments arranged by size:
940 505 334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1779
Size: 190 1589
BsaI GGTCTCn'nnnn_
Cuts at: 0 1492 1779
Size: 1492 287
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1452 1482 1779
Size: 1452 30 297
Fragments arranged by size:
1452 297 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 1779
Size: 493 1286
BseYI C'CCAG_C
Cuts at: 0 57 807 812 1015 1779
Size: 57 750 5 203 764
Fragments arranged by size:
764 750 203 57 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 1779
Size: 1166 613
BsmI GAATG_Cn'
Cuts at: 0 618 1779
Size: 618 1161
Bsp1286I G_dGCh'C
Cuts at: 0 693 835 1263 1779
Size: 693 142 428 516
Fragments arranged by size:
693 516 428 142
BspHI T'CATG_A
Cuts at: 0 1505 1779
Size: 1505 274
BsrBI CCG'CTC
Cuts at: 0 483 1580 1779
Size: 483 1097 199
Fragments arranged by size:
1097 483 199
BsrDI GCAATG_nn'
Cuts at: 0 139 1655 1779
Size: 139 1516 124
Fragments arranged by size:
1516 139 124
BsrFI r'CCGG_y
Cuts at: 0 1376 1779
Size: 1376 403
BsrGI T'GTAC_A
Cuts at: 0 606 1779
Size: 606 1173
BstYI r'GATC_y
Cuts at: 0 1097 1779
Size: 1097 682
BtgI C'CryG_G
Cuts at: 0 116 1779
Size: 116 1663
BtsI GCAGTG_nn'
Cuts at: 0 213 1333 1779
Size: 213 1120 446
Fragments arranged by size:
1120 446 213
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 758 793 1779
Size: 758 35 986
Fragments arranged by size:
986 758 35
EarI CTCTTCn'nnn_
Cuts at: 0 29 1070 1779
Size: 29 1041 709
Fragments arranged by size:
1041 709 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1114 1454 1537 1779
Size: 584 530 340 83 242
Fragments arranged by size:
584 530 340 242 83
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1408 1779
Size: 1408 371
EcoO109I rG'GnC_Cy
Cuts at: 0 660 689 831 1693 1779
Size: 660 29 142 862 86
Fragments arranged by size:
862 660 142 86 29
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 40 66 250 1779
Size: 40 26 184 1529
Fragments arranged by size:
1529 184 40 26
HaeII r_GCGC'y
Cuts at: 0 1022 1779
Size: 1022 757
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 549 581 1779
Size: 62 32 455 32 1198
Fragments arranged by size:
1198 455 62 32 32
HincII GTy'rAC
Cuts at: 0 1653 1779
Size: 1653 126
HindIII A'AGCT_T
Cuts at: 0 670 1779
Size: 670 1109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1364 1779
Size: 1364 415
MslI CAynn'nnrTG
Cuts at: 0 778 949 1168 1779
Size: 778 171 219 611
Fragments arranged by size:
778 611 219 171
MspA1I CmG'CkG
Cuts at: 0 504 828 1077 1474 1779
Size: 504 324 249 397 305
Fragments arranged by size:
504 397 324 305 249
NcoI C'CATG_G
Cuts at: 0 116 1779
Size: 116 1663
NsiI A_TGCA'T
Cuts at: 0 1392 1779
Size: 1392 387
NspI r_CATG'y
Cuts at: 0 777 1779
Size: 777 1002
PciI A'CATG_T
Cuts at: 0 773 1779
Size: 773 1006
PfoI T'CCnGG_A
Cuts at: 0 1091 1779
Size: 1091 688
PspOMI G'GGCC_C
Cuts at: 0 689 831 1779
Size: 689 142 948
Fragments arranged by size:
948 689 142
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1418 1450 1779
Size: 1418 32 329
Fragments arranged by size:
1418 329 32
PstI C_TGCA'G
Cuts at: 0 1053 1779
Size: 1053 726
PvuII CAG'CTG
Cuts at: 0 504 1077 1474 1779
Size: 504 573 397 305
Fragments arranged by size:
573 504 397 305
SapI GCTCTTCn'nnn_
Cuts at: 0 29 1070 1779
Size: 29 1041 709
Fragments arranged by size:
1041 709 29
SfcI C'TryA_G
Cuts at: 0 571 817 1049 1405 1779
Size: 571 246 232 356 374
Fragments arranged by size:
571 374 356 246 232
SmlI C'TyrA_G
Cuts at: 0 169 1779
Size: 169 1610
StuI AGG'CCT
Cuts at: 0 252 1779
Size: 252 1527
StyI C'CwwG_G
Cuts at: 0 116 693 1212 1635 1779
Size: 116 577 519 423 144
Fragments arranged by size:
577 519 423 144 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 882 1724 1779
Size: 882 842 55
Fragments arranged by size:
882 842 55
TatI w'GTAC_w
Cuts at: 0 606 1779
Size: 606 1173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1452 1484 1779
Size: 1452 32 295
Fragments arranged by size:
1452 295 32
Tth111I GACn'n_nGTC
Cuts at: 0 563 1779
Size: 563 1216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 420 1779
Size: 420 1359
Enzymes that do cut:
AflIII AloI ApaI ApoI BanI BanII BbsI BbvCI
BfrBI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BsrBI
BsrDI BsrFI BsrGI BstYI BtgI BtsI CspCI EarI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII
MmeI MslI MspA1I NcoI NsiI NspI PciI PfoI
PspOMI PsrI PstI PvuII SapI SfcI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmaI XmnI ZraI