Restriction map for
pF1KA1147
(Linear) (Six-base) MAPSORT of: KIAA1147.seq Check: 5017 from: 1 to: 1365
KIAA1147.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1046 1365
Size: 1046 319
AatII G_ACGT'C
Cuts at: 0 998 1365
Size: 998 367
AccI GT'mk_AC
Cuts at: 0 635 998 1365
Size: 635 363 367
Fragments arranged by size:
635 367 363
AclI AA'CG_TT
Cuts at: 0 845 1365
Size: 845 520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 1365
Size: 1072 293
AflIII A'CryG_T
Cuts at: 0 662 1365
Size: 662 703
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 640 1365
Size: 640 725
AlwNI CAG_nnn'CTG
Cuts at: 0 1047 1110 1365
Size: 1047 63 255
Fragments arranged by size:
1047 255 63
ApaI G_GGCC'C
Cuts at: 0 49 386 1365
Size: 49 337 979
Fragments arranged by size:
979 337 49
ApoI r'AATT_y
Cuts at: 0 751 1365
Size: 751 614
AvaI C'yCGr_G
Cuts at: 0 154 190 264 1259 1365
Size: 154 36 74 995 106
Fragments arranged by size:
995 154 106 74 36
BanI G'GyrC_C
Cuts at: 0 21 115 194 527 868 1365
Size: 21 94 79 333 341 497
Fragments arranged by size:
497 341 333 94 79 21
BanII G_rGCy'C
Cuts at: 0 49 155 386 1365
Size: 49 106 231 979
Fragments arranged by size:
979 231 106 49
BbeI G_GCGC'C
Cuts at: 0 25 119 198 1365
Size: 25 94 79 1167
Fragments arranged by size:
1167 94 79 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1027 1142 1365
Size: 1027 115 223
Fragments arranged by size:
1027 223 115
BglI GCCn_nnn'nGGC
Cuts at: 0 104 124 144 1365
Size: 104 20 20 1221
Fragments arranged by size:
1221 104 20 20
Bme1580I G_kGCm'C
Cuts at: 0 49 386 871 1365
Size: 49 337 485 494
Fragments arranged by size:
494 485 337 49
BmgBI CAC'GTC
Cuts at: 0 213 1365
Size: 213 1152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 946 1333 1365
Size: 220 726 387 32
Fragments arranged by size:
726 387 220 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 1365
Size: 278 1087
BsaI GGTCTCn'nnnn_
Cuts at: 0 1280 1365
Size: 1280 85
BsaAI yAC'GTr
Cuts at: 0 900 1002 1365
Size: 900 102 363
Fragments arranged by size:
900 363 102
BsaBI GATnn'nnATC
Cuts at: 0 679 739 1365
Size: 679 60 626
Fragments arranged by size:
679 626 60
BsaHI Gr'CG_yC
Cuts at: 0 22 116 195 995 1365
Size: 22 94 79 800 370
Fragments arranged by size:
800 370 94 79 22
BsaWI w'CCGG_w
Cuts at: 0 1193 1289 1365
Size: 1193 96 76
Fragments arranged by size:
1193 96 76
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 232 988 1365
Size: 172 60 756 377
Fragments arranged by size:
756 377 172 60
BseYI C'CCAG_C
Cuts at: 0 35 92 204 690 1365
Size: 35 57 112 486 675
Fragments arranged by size:
675 486 112 57 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1365
Size: 936 429
BsiEI CG_ry'CG
Cuts at: 0 124 128 167 1365
Size: 124 4 39 1198
Fragments arranged by size:
1198 124 39 4
BsiHKAI G_wGCw'C
Cuts at: 0 596 1365
Size: 596 769
BsmBI CGTCTCn'nnnn_
Cuts at: 0 8 60 1365
Size: 8 52 1305
Fragments arranged by size:
1305 52 8
Bsp1286I G_dGCh'C
Cuts at: 0 49 155 386 596 871 1365
Size: 49 106 231 210 275 494
Fragments arranged by size:
494 275 231 210 106 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 1316 1365
Size: 1046 270 49
Fragments arranged by size:
1046 270 49
BsrBI CCG'CTC
Cuts at: 0 263 1365
Size: 263 1102
BsrFI r'CCGG_y
Cuts at: 0 123 162 1085 1365
Size: 123 39 923 280
Fragments arranged by size:
923 280 123 39
BssHII G'CGCG_C
Cuts at: 0 443 1365
Size: 443 922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1047 1255 1365
Size: 1047 208 110
Fragments arranged by size:
1047 208 110
BstBI TT'CG_AA
Cuts at: 0 765 1365
Size: 765 600
BstEII G'GTnAC_C
Cuts at: 0 246 1365
Size: 246 1119
BstYI r'GATC_y
Cuts at: 0 734 1365
Size: 734 631
BtgI C'CryG_G
Cuts at: 0 128 419 1365
Size: 128 291 946
Fragments arranged by size:
946 291 128
BtsI GCAGTG_nn'
Cuts at: 0 484 850 1365
Size: 484 366 515
Fragments arranged by size:
515 484 366
EaeI y'GGCC_r
Cuts at: 0 121 125 164 233 332 839 1365
Size: 121 4 39 69 99 507 526
Fragments arranged by size:
526 507 121 99 69 39 4
EagI C'GGCC_G
Cuts at: 0 121 125 164 1365
Size: 121 4 39 1201
Fragments arranged by size:
1201 121 39 4
EarI CTCTTCn'nnn_
Cuts at: 0 1148 1168 1365
Size: 1148 20 197
Fragments arranged by size:
1148 197 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 946 1072 1333 1365
Size: 220 726 126 261 32
Fragments arranged by size:
726 261 220 126 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 64 1312 1365
Size: 64 1248 53
Fragments arranged by size:
1248 64 53
EcoO109I rG'GnC_Cy
Cuts at: 0 45 46 69 341 382 383 1158 1365
Size: 45 1 23 272 41 1 775 207
Fragments arranged by size:
775 272 207 45 41 23 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 402 1365
Size: 402 963
FseI GG_CCGG'CC
Cuts at: 0 127 1365
Size: 127 1238
HaeII r_GCGC'y
Cuts at: 0 25 119 198 1365
Size: 25 94 79 1167
Fragments arranged by size:
1167 94 79 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 310 342 719 751 771 803
Size: 247 32 31 32 377 32 20 32
Cuts at: 803 1365
Size: 562
Fragments arranged by size:
562 377 247 32 32 32 32 31
20
KasI G'GCGC_C
Cuts at: 0 21 115 194 1365
Size: 21 94 79 1171
Fragments arranged by size:
1171 94 79 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1193 1365
Size: 515 678 172
Fragments arranged by size:
678 515 172
MscI TGG'CCA
Cuts at: 0 334 841 1365
Size: 334 507 524
Fragments arranged by size:
524 507 334
MslI CAynn'nnrTG
Cuts at: 0 418 1365
Size: 418 947
MspA1I CmG'CkG
Cuts at: 0 27 130 694 1047 1365
Size: 27 103 564 353 318
Fragments arranged by size:
564 353 318 103 27
NaeI GCC'GGC
Cuts at: 0 125 164 1087 1365
Size: 125 39 923 278
Fragments arranged by size:
923 278 125 39
NarI GG'CG_CC
Cuts at: 0 22 116 195 1365
Size: 22 94 79 1170
Fragments arranged by size:
1170 94 79 22
NgoMIV G'CCGG_C
Cuts at: 0 123 162 1085 1365
Size: 123 39 923 280
Fragments arranged by size:
923 280 123 39
NspI r_CATG'y
Cuts at: 0 417 504 666 705 1258 1365
Size: 417 87 162 39 553 107
Fragments arranged by size:
553 417 162 107 87 39
PciI A'CATG_T
Cuts at: 0 662 1365
Size: 662 703
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 1365
Size: 231 1134
PfoI T'CCnGG_A
Cuts at: 0 1301 1365
Size: 1301 64
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 722 754 1365
Size: 722 32 611
Fragments arranged by size:
722 611 32
PpuMI rG'GwC_Cy
Cuts at: 0 341 1158 1365
Size: 341 817 207
Fragments arranged by size:
817 341 207
PsiI TTA'TAA
Cuts at: 0 715 1365
Size: 715 650
PspOMI G'GGCC_C
Cuts at: 0 45 382 1365
Size: 45 337 983
Fragments arranged by size:
983 337 45
PstI C_TGCA'G
Cuts at: 0 188 1043 1365
Size: 188 855 322
Fragments arranged by size:
855 322 188
PvuII CAG'CTG
Cuts at: 0 694 1365
Size: 694 671
SacII CC_GC'GG
Cuts at: 0 131 1365
Size: 131 1234
SanDI GG'GwC_CC
Cuts at: 0 341 1365
Size: 341 1024
SexAI A'CCwGG_T
Cuts at: 0 224 518 1365
Size: 224 294 847
Fragments arranged by size:
847 294 224
SfcI C'TryA_G
Cuts at: 0 184 813 1039 1365
Size: 184 629 226 326
Fragments arranged by size:
629 326 226 184
SfoI GGC'GCC
Cuts at: 0 23 117 196 1365
Size: 23 94 79 1169
Fragments arranged by size:
1169 94 79 23
SmaI CCC'GGG
Cuts at: 0 192 1261 1365
Size: 192 1069 104
Fragments arranged by size:
1069 192 104
SmlI C'TyrA_G
Cuts at: 0 293 1365
Size: 293 1072
SphI G_CATG'C
Cuts at: 0 1258 1365
Size: 1258 107
SrfI GCCC'GGGC
Cuts at: 0 192 1365
Size: 192 1173
StuI AGG'CCT
Cuts at: 0 1318 1365
Size: 1318 47
StyI C'CwwG_G
Cuts at: 0 1281 1365
Size: 1281 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 704 1365
Size: 704 661
TatI w'GTAC_w
Cuts at: 0 821 1115 1365
Size: 821 294 250
Fragments arranged by size:
821 294 250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 417 1365
Size: 417 948
XmaI C'CCGG_G
Cuts at: 0 190 1259 1365
Size: 190 1069 106
Fragments arranged by size:
1069 190 106
XmnI GAAnn'nnTTC
Cuts at: 0 967 1365
Size: 967 398
ZraI GAC'GTC
Cuts at: 0 996 1365
Size: 996 369
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BglI
Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstYI
BtgI BtsI EaeI EagI EarI Eco57MI EcoNI EcoO109I
FalI FseI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PpiI PpuMI PsiI PspOMI PstI PvuII SacII SanDI
SexAI SfcI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI
Bpu10I BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BssSI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoICRI EcoRI EcoRV FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PshAI PspXI PsrI PvuI RsrII SacI SalI SapI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SspI
SwaI TstI Tth111I XbaI XhoI