Restriction map for
pF1KA1141
(Linear) (Six-base) MAPSORT of: KIAA1141.seq Check: 5115 from: 1 to: 2613
KIAA1141.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AclI AA'CG_TT
Cuts at: 0 2128 2535 2613
Size: 2128 407 78
Fragments arranged by size:
2128 407 78
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 1218 1609 1891 1947 2613
Size: 636 582 391 282 56 666
Fragments arranged by size:
666 636 582 391 282 56
AflIII A'CryG_T
Cuts at: 0 86 2613
Size: 86 2527
AleI CACnn'nnGTG
Cuts at: 0 1601 2174 2613
Size: 1601 573 439
Fragments arranged by size:
1601 573 439
AlwNI CAG_nnn'CTG
Cuts at: 0 2496 2613
Size: 2496 117
ApaLI G'TGCA_C
Cuts at: 0 1973 2613
Size: 1973 640
ApoI r'AATT_y
Cuts at: 0 10 787 1352 2165 2249 2417 2613
Size: 10 777 565 813 84 168 196
Fragments arranged by size:
813 777 565 196 168 84 10
AvaI C'yCGr_G
Cuts at: 0 67 2562 2613
Size: 67 2495 51
Fragments arranged by size:
2495 67 51
BanI G'GyrC_C
Cuts at: 0 1010 2555 2613
Size: 1010 1545 58
Fragments arranged by size:
1545 1010 58
BanII G_rGCy'C
Cuts at: 0 2143 2311 2613
Size: 2143 168 302
Fragments arranged by size:
2143 302 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 385 2037 2613
Size: 385 1652 576
Fragments arranged by size:
1652 576 385
BbvCI CC'TCA_GC
Cuts at: 0 5 2569 2613
Size: 5 2564 44
Fragments arranged by size:
2564 44 5
BciVI GTATCCnnnnn_n'
Cuts at: 0 773 2613
Size: 773 1840
BglII A'GATC_T
Cuts at: 0 180 1646 2613
Size: 180 1466 967
Fragments arranged by size:
1466 967 180
BlpI GC'TnA_GC
Cuts at: 0 2228 2613
Size: 2228 385
Bme1580I G_kGCm'C
Cuts at: 0 1977 2481 2613
Size: 1977 504 132
Fragments arranged by size:
1977 504 132
BmrI ACTGGGnnnn_n'
Cuts at: 0 66 896 2351 2613
Size: 66 830 1455 262
Fragments arranged by size:
1455 830 262 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 588 2613
Size: 588 2025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 881 1214 2027 2279 2613
Size: 205 676 333 813 252 334
Fragments arranged by size:
813 676 334 333 252 205
Bpu10I CC'TnA_GC
Cuts at: 0 5 2569 2613
Size: 5 2564 44
Fragments arranged by size:
2564 44 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 1092 2613
Size: 7 1085 1521
Fragments arranged by size:
1521 1085 7
BsaI GGTCTCn'nnnn_
Cuts at: 0 229 2613
Size: 229 2384
BsaBI GATnn'nnATC
Cuts at: 0 1496 2613
Size: 1496 1117
BsaWI w'CCGG_w
Cuts at: 0 740 2327 2613
Size: 740 1587 286
Fragments arranged by size:
1587 740 286
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 651 681 2613
Size: 142 30 479 30 1932
Fragments arranged by size:
1932 479 142 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 239 1213 2613
Size: 239 974 1400
Fragments arranged by size:
1400 974 239
BseYI C'CCAG_C
Cuts at: 0 674 2613
Size: 674 1939
BsiHKAI G_wGCw'C
Cuts at: 0 1931 1977 2143 2311 2613
Size: 1931 46 166 168 302
Fragments arranged by size:
1931 302 168 166 46
BsmI GAATG_Cn'
Cuts at: 0 442 750 976 1393 1477 1954 2122 2290
Size: 442 308 226 417 84 477 168 168
Cuts at: 2290 2613
Size: 323
Fragments arranged by size:
477 442 417 323 308 226 168 168
84
Bsp1286I G_dGCh'C
Cuts at: 0 1931 1977 2143 2311 2481 2613
Size: 1931 46 166 168 170 132
Fragments arranged by size:
1931 170 168 166 132 46
BspHI T'CATG_A
Cuts at: 0 447 906 2613
Size: 447 459 1707
Fragments arranged by size:
1707 459 447
BsrFI r'CCGG_y
Cuts at: 0 1322 1406 2613
Size: 1322 84 1207
Fragments arranged by size:
1322 1207 84
BsrGI T'GTAC_A
Cuts at: 0 2586 2613
Size: 2586 27
BssSI C'ACGA_G
Cuts at: 0 1597 1738 2613
Size: 1597 141 875
Fragments arranged by size:
1597 875 141
BstBI TT'CG_AA
Cuts at: 0 269 2613
Size: 269 2344
BstXI CCAn_nnnn'nTGG
Cuts at: 0 169 2613
Size: 169 2444
BstYI r'GATC_y
Cuts at: 0 180 992 1019 1256 1646 2613
Size: 180 812 27 237 390 967
Fragments arranged by size:
967 812 390 237 180 27
BtgI C'CryG_G
Cuts at: 0 431 488 503 2613
Size: 431 57 15 2110
Fragments arranged by size:
2110 431 57 15
BtsI GCAGTG_nn'
Cuts at: 0 180 1305 1893 2613
Size: 180 1125 588 720
Fragments arranged by size:
1125 720 588 180
DraI TTT'AAA
Cuts at: 0 1938 2022 2613
Size: 1938 84 591
Fragments arranged by size:
1938 591 84
EaeI y'GGCC_r
Cuts at: 0 1800 2613
Size: 1800 813
EarI CTCTTCn'nnn_
Cuts at: 0 130 1190 2613
Size: 130 1060 1423
Fragments arranged by size:
1423 1060 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2227 2613
Size: 2227 386
EcoICRI GAG'CTC
Cuts at: 0 2141 2309 2613
Size: 2141 168 304
Fragments arranged by size:
2141 304 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 636 881 1214 1218 1609 1891 1947
Size: 205 431 245 333 4 391 282 56
Cuts at: 1947 2027 2279 2613
Size: 80 252 334
Fragments arranged by size:
431 391 334 333 282 252 245 205
80 56 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 194 2613
Size: 194 2419
EcoO109I rG'GnC_Cy
Cuts at: 0 120 2613
Size: 120 2493
EcoRI G'AATT_C
Cuts at: 0 1352 2165 2249 2417 2613
Size: 1352 813 84 168 196
Fragments arranged by size:
1352 813 196 168 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 333 2613
Size: 333 2280
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 651 683 937 969 1011 1043
Size: 289 32 330 32 254 32 42 32
Cuts at: 1043 1413 1445 1638 1670 2508 2540 2613
Size: 370 32 193 32 838 32 73
Fragments arranged by size:
838 370 330 289 254 193 73 42
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2533 2613
Size: 2533 80
HindIII A'AGCT_T
Cuts at: 0 648 2462 2613
Size: 648 1814 151
Fragments arranged by size:
1814 648 151
MfeI C'AATT_G
Cuts at: 0 111 2613
Size: 111 2502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 605 1113 2613
Size: 86 519 508 1500
Fragments arranged by size:
1500 519 508 86
MscI TGG'CCA
Cuts at: 0 1802 2613
Size: 1802 811
MslI CAynn'nnrTG
Cuts at: 0 167 627 837 1337 1545 1601 2174 2582
Size: 167 460 210 500 208 56 573 408
Cuts at: 2582 2613
Size: 31
Fragments arranged by size:
573 500 460 408 210 208 167 56
31
NaeI GCC'GGC
Cuts at: 0 1408 2613
Size: 1408 1205
NcoI C'CATG_G
Cuts at: 0 503 2613
Size: 503 2110
NdeI CA'TA_TG
Cuts at: 0 1542 2613
Size: 1542 1071
NgoMIV G'CCGG_C
Cuts at: 0 1406 2613
Size: 1406 1207
PpuMI rG'GwC_Cy
Cuts at: 0 120 2613
Size: 120 2493
PsiI TTA'TAA
Cuts at: 0 1208 2613
Size: 1208 1405
PspXI vC'TCGA_Gb
Cuts at: 0 2562 2613
Size: 2562 51
PstI C_TGCA'G
Cuts at: 0 1720 2613
Size: 1720 893
SacI G_AGCT'C
Cuts at: 0 2143 2311 2613
Size: 2143 168 302
Fragments arranged by size:
2143 302 168
SapI GCTCTTCn'nnn_
Cuts at: 0 1190 2613
Size: 1190 1423
ScaI AGT'ACT
Cuts at: 0 1809 2613
Size: 1809 804
SexAI A'CCwGG_T
Cuts at: 0 358 2613
Size: 358 2255
SfcI C'TryA_G
Cuts at: 0 1365 1458 1716 2355 2613
Size: 1365 93 258 639 258
Fragments arranged by size:
1365 639 258 258 93
SmlI C'TyrA_G
Cuts at: 0 22 1071 2562 2613
Size: 22 1049 1491 51
Fragments arranged by size:
1491 1049 51 22
StuI AGG'CCT
Cuts at: 0 1315 2212 2613
Size: 1315 897 401
Fragments arranged by size:
1315 897 401
StyI C'CwwG_G
Cuts at: 0 47 308 503 2613
Size: 47 261 195 2110
Fragments arranged by size:
2110 261 195 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 179 911 915 2065 2317 2524 2613
Size: 179 732 4 1150 252 207 89
Fragments arranged by size:
1150 732 252 207 179 89 4
TatI w'GTAC_w
Cuts at: 0 829 1807 2586 2613
Size: 829 978 779 27
Fragments arranged by size:
978 829 779 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1619 1651 2613
Size: 1619 32 962
Fragments arranged by size:
1619 962 32
Tth111I GACn'n_nGTC
Cuts at: 0 467 2041 2613
Size: 467 1574 572
Fragments arranged by size:
1574 572 467
XbaI T'CTAG_A
Cuts at: 0 1840 2613
Size: 1840 773
XhoI C'TCGA_G
Cuts at: 0 2562 2613
Size: 2562 51
XmnI GAAnn'nnTTC
Cuts at: 0 392 649 1231 1315 2128 2212 2296 2613
Size: 392 257 582 84 813 84 84 317
Fragments arranged by size:
813 582 392 317 257 84 84 84
Enzymes that do cut:
AclI AcuI AflIII AleI AlwNI ApaLI ApoI AvaI
BanI BanII BbsI BbvCI BciVI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrFI
BsrGI BssSI BstBI BstXI BstYI BtgI BtsI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I HincII HindIII MfeI MmeI MscI MslI
NaeI NcoI NdeI NgoMIV PpuMI PsiI PspXI PstI
SacI SapI ScaI SexAI SfcI SmlI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BclI BfrBI BglI BmgBI BmtI
BsaAI BsaHI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrDI BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EagI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MluI MspA1I NarI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI XcmI XmaI ZraI