Restriction map for
pF1KSDA1138
(Linear) (Six-base) MAPSORT of: KIAA1138.seq Check: 8593 from: 1 to: 3663
KIAA1138.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 816 1161 3663
Size: 816 345 2502
Fragments arranged by size:
2502 816 345
AatII G_ACGT'C
Cuts at: 0 3374 3663
Size: 3374 289
AccI GT'mk_AC
Cuts at: 0 3245 3663
Size: 3245 418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1552 2936 3472 3663
Size: 1552 1384 536 191
Fragments arranged by size:
1552 1384 536 191
AfeI AGC'GCT
Cuts at: 0 2458 2631 3402 3663
Size: 2458 173 771 261
Fragments arranged by size:
2458 771 261 173
AflIII A'CryG_T
Cuts at: 0 3041 3093 3479 3663
Size: 3041 52 386 184
Fragments arranged by size:
3041 386 184 52
AleI CACnn'nnGTG
Cuts at: 0 1324 3253 3277 3472 3484 3663
Size: 1324 1929 24 195 12 179
Fragments arranged by size:
1929 1324 195 179 24 12
AlwNI CAG_nnn'CTG
Cuts at: 0 2307 2915 3663
Size: 2307 608 748
Fragments arranged by size:
2307 748 608
ApaI G_GGCC'C
Cuts at: 0 68 108 115 418 1149 1179 1252 1545
Size: 68 40 7 303 731 30 73 293
Cuts at: 1545 2094 3663
Size: 549 1569
Fragments arranged by size:
1569 731 549 303 293 73 68 40
30 7
ApaLI G'TGCA_C
Cuts at: 0 3256 3663
Size: 3256 407
AscI GG'CGCG_CC
Cuts at: 0 119 3663
Size: 119 3544
AvaI C'yCGr_G
Cuts at: 0 58 107 749 776 1969 2025 2744 2854
Size: 58 49 642 27 1193 56 719 110
Cuts at: 2854 3171 3663
Size: 317 492
Fragments arranged by size:
1193 719 642 492 317 110 58 56
49 27
BanI G'GyrC_C
Cuts at: 0 54 95 141 217 381 589 728 1507
Size: 54 41 46 76 164 208 139 779
Cuts at: 1507 1602 2043 2239 2929 3607 3663
Size: 95 441 196 690 678 56
Fragments arranged by size:
779 690 678 441 208 196 164 139
95 76 56 54 46 41
BanII G_rGCy'C
Cuts at: 0 68 108 115 418 784 800 902 1149
Size: 68 40 7 303 366 16 102 247
Cuts at: 1149 1179 1252 1378 1545 2024 2094 3155 3663
Size: 30 73 126 167 479 70 1061 508
Fragments arranged by size:
1061 508 479 366 303 247 167 126
102 73 70 68 40 30 16 7
BbeI G_GCGC'C
Cuts at: 0 58 145 385 1511 3611 3663
Size: 58 87 240 1126 2100 52
Fragments arranged by size:
2100 1126 240 87 58 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 3142 3650 3663
Size: 3142 508 13
Fragments arranged by size:
3142 508 13
BbvCI CC'TCA_GC
Cuts at: 0 733 1636 2644 2879 3663
Size: 733 903 1008 235 784
Fragments arranged by size:
1008 903 784 733 235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2106 2135 2140 2169 3663
Size: 2106 29 5 29 1494
Fragments arranged by size:
2106 1494 29 29 5
BglI GCCn_nnn'nGGC
Cuts at: 0 63 72 286 630 1356 1365 1704 2098
Size: 63 9 214 344 726 9 339 394
Cuts at: 2098 2143 2927 3125 3663
Size: 45 784 198 538
Fragments arranged by size:
784 726 538 394 344 339 214 198
63 45 9 9
BlpI GC'TnA_GC
Cuts at: 0 2725 3388 3663
Size: 2725 663 275
Fragments arranged by size:
2725 663 275
Bme1580I G_kGCm'C
Cuts at: 0 68 108 115 418 594 1149 1179 1252
Size: 68 40 7 303 176 555 30 73
Cuts at: 1252 1545 2048 2094 2639 3260 3587 3663
Size: 293 503 46 545 621 327 76
Fragments arranged by size:
621 555 545 503 327 303 293 176
76 73 68 46 40 30 7
BmgBI CAC'GTC
Cuts at: 0 1035 3255 3482 3663
Size: 1035 2220 227 181
Fragments arranged by size:
2220 1035 227 181
BmrI ACTGGGnnnn_n'
Cuts at: 0 644 3000 3030 3663
Size: 644 2356 30 633
Fragments arranged by size:
2356 644 633 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 375 1527 3663
Size: 375 1152 2136
Fragments arranged by size:
2136 1152 375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 331 358 660 715 1000 1030 1414
Size: 277 54 27 302 55 285 30 384
Cuts at: 1414 1650 1755 1825 3241 3451 3576 3663
Size: 236 105 70 1416 210 125 87
Fragments arranged by size:
1416 384 302 285 277 236 210 125
105 87 70 55 54 30 27
Bpu10I CC'TnA_GC
Cuts at: 0 182 733 1546 1636 2288 2363 2611 2644
Size: 182 551 813 90 652 75 248 33
Cuts at: 2644 2879 3663
Size: 235 784
Fragments arranged by size:
813 784 652 551 248 235 182 90
75 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1693 2473 2587 2800 3142 3663
Size: 1693 780 114 213 342 521
Fragments arranged by size:
1693 780 521 342 213 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 1000 3025 3663
Size: 1000 2025 638
Fragments arranged by size:
2025 1000 638
BsaAI yAC'GTr
Cuts at: 0 663 3096 3261 3663
Size: 663 2433 165 402
Fragments arranged by size:
2433 663 402 165
BsaHI Gr'CG_yC
Cuts at: 0 55 142 382 547 1508 1520 2335 3371
Size: 55 87 240 165 961 12 815 1036
Cuts at: 3371 3602 3608 3647 3663
Size: 231 6 39 16
Fragments arranged by size:
1036 961 815 240 231 165 87 55
39 16 12 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 795 825 1636 1648 1666 1678
Size: 694 30 71 30 811 12 18 12
Cuts at: 1678 1810 1840 3022 3052 3663
Size: 132 30 1182 30 611
Fragments arranged by size:
1182 811 694 611 132 71 30 30
30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 1734 1737 1740 1743 1746 1749 1752
Size: 250 1484 3 3 3 3 3 3
Cuts at: 1752 1855 1948 1995 2043 2205 2494 2551 2896
Size: 103 93 47 48 162 289 57 345
Cuts at: 2896 2989 3663
Size: 93 674
Fragments arranged by size:
1484 674 345 289 250 162 103 93
93 57 48 47 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 159 1056 1074 1159 1630 2093 2563 2626
Size: 159 897 18 85 471 463 470 63
Cuts at: 2626 3026 3663
Size: 400 637
Fragments arranged by size:
897 637 471 470 463 400 159 85
63 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 3118 3663
Size: 1045 2073 545
Fragments arranged by size:
2073 1045 545
BsiEI CG_ry'CG
Cuts at: 0 1022 1288 1372 1399 1406 3663
Size: 1022 266 84 27 7 2257
Fragments arranged by size:
2257 1022 266 84 27 7
BsiHKAI G_wGCw'C
Cuts at: 0 356 1453 3155 3260 3491 3663
Size: 356 1097 1702 105 231 172
Fragments arranged by size:
1702 1097 356 231 172 105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 334 1009 3663
Size: 334 675 2654
Fragments arranged by size:
2654 675 334
Bsp1286I G_dGCh'C
Cuts at: 0 68 108 115 356 418 594 784 800
Size: 68 40 7 241 62 176 190 16
Cuts at: 800 902 1149 1179 1252 1378 1453 1545 2024
Size: 102 247 30 73 126 75 92 479
Cuts at: 2024 2048 2094 2639 3155 3260 3491 3587 3663
Size: 24 46 545 516 105 231 96 76
Fragments arranged by size:
545 516 479 247 241 231 190 176
126 105 102 96 92 76 75 73
68 62 46 40 30 24 16 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 816 1161 2093 2687 2804 2923 3663
Size: 816 345 932 594 117 119 740
Fragments arranged by size:
932 816 740 594 345 119 117
BsrBI CCG'CTC
Cuts at: 0 359 759 1284 1482 1493 2850 3663
Size: 359 400 525 198 11 1357 813
Fragments arranged by size:
1357 813 525 400 359 198 11
BsrFI r'CCGG_y
Cuts at: 0 71 487 1355 1398 1405 1895 3061 3604
Size: 71 416 868 43 7 490 1166 543
Cuts at: 3604 3663
Size: 59
Fragments arranged by size:
1166 868 543 490 416 71 59 43
7
BsrGI T'GTAC_A
Cuts at: 0 2774 3663
Size: 2774 889
BssHII G'CGCG_C
Cuts at: 0 119 3663
Size: 119 3544
BssSI C'ACGA_G
Cuts at: 0 2677 2941 3663
Size: 2677 264 722
Fragments arranged by size:
2677 722 264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2915 3096 3663
Size: 2915 181 567
Fragments arranged by size:
2915 567 181
BstXI CCAn_nnnn'nTGG
Cuts at: 0 507 693 1326 3663
Size: 507 186 633 2337
Fragments arranged by size:
2337 633 507 186
BstYI r'GATC_y
Cuts at: 0 1853 1985 3176 3663
Size: 1853 132 1191 487
Fragments arranged by size:
1853 1191 487 132
Bsu36I CC'TnA_GG
Cuts at: 0 1335 2785 3663
Size: 1335 1450 878
Fragments arranged by size:
1450 1335 878
BtgI C'CryG_G
Cuts at: 0 99 387 465 786 881 905 1835 2039
Size: 99 288 78 321 95 24 930 204
Cuts at: 2039 3470 3663
Size: 1431 193
Fragments arranged by size:
1431 930 321 288 204 193 99 95
78 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 860 3663
Size: 860 2803
BtsI GCAGTG_nn'
Cuts at: 0 303 798 1033 3663
Size: 303 495 235 2630
Fragments arranged by size:
2630 495 303 235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2637 2672 3663
Size: 2637 35 991
Fragments arranged by size:
2637 991 35
DraI TTT'AAA
Cuts at: 0 2399 3663
Size: 2399 1264
DraIII CAC_nnn'GTG
Cuts at: 0 2682 3624 3663
Size: 2682 942 39
Fragments arranged by size:
2682 942 39
DrdI GACnn_nn'nnGTC
Cuts at: 0 3355 3663
Size: 3355 308
EaeI y'GGCC_r
Cuts at: 0 509 908 1019 1285 1316 1328 1357 1369
Size: 509 399 111 266 31 12 29 12
Cuts at: 1369 1403 2258 2298 2588 2658 2702 3017 3105
Size: 34 855 40 290 70 44 315 88
Cuts at: 3105 3542 3663
Size: 437 121
Fragments arranged by size:
855 509 437 399 315 290 266 121
111 88 70 44 40 34 31 29
12 12
EagI C'GGCC_G
Cuts at: 0 1019 1285 1369 1403 3663
Size: 1019 266 84 34 2260
Fragments arranged by size:
2260 1019 266 84 34
EarI CTCTTCn'nnn_
Cuts at: 0 1441 1916 1921 1972 2182 2881 2907 2955
Size: 1441 475 5 51 210 699 26 48
Cuts at: 2955 3496 3663
Size: 541 167
Fragments arranged by size:
1441 699 541 475 210 167 51 48
26 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 149 351 1798 2163 2440 3663
Size: 149 202 1447 365 277 1223
Fragments arranged by size:
1447 1223 365 277 202 149
EcoICRI GAG'CTC
Cuts at: 0 3153 3663
Size: 3153 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 331 358 660 715 1000 1030 1414
Size: 277 54 27 302 55 285 30 384
Cuts at: 1414 1552 1650 1755 1825 2936 3241 3451 3472
Size: 138 98 105 70 1111 305 210 21
Cuts at: 3472 3576 3663
Size: 104 87
Fragments arranged by size:
1111 384 305 302 285 277 210 138
105 104 98 87 70 55 54 30
27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 232 1807 2291 3663
Size: 232 1575 484 1372
Fragments arranged by size:
1575 1372 484 232
EcoO109I rG'GnC_Cy
Cuts at: 0 64 65 104 111 112 369 975 1041
Size: 64 1 39 7 1 257 606 66
Cuts at: 1041 1145 1146 1175 1248 1458 1542 2035 2130
Size: 104 1 29 73 210 84 493 95
Cuts at: 2130 2249 2616 2754 2912 3663
Size: 119 367 138 158 751
Fragments arranged by size:
751 606 493 367 257 210 158 138
119 104 95 84 73 66 64 39
29 7 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2733 3663
Size: 2733 930
FspI TGC'GCA
Cuts at: 0 295 3663
Size: 295 3368
HaeII r_GCGC'y
Cuts at: 0 58 145 324 385 1511 2460 2633 3120
Size: 58 87 179 61 1126 949 173 487
Cuts at: 3120 3404 3611 3663
Size: 284 207 52
Fragments arranged by size:
1126 949 487 284 207 179 173 87
61 58 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 1636 1642 1648 1668 1674 1680
Size: 694 32 910 6 6 20 6 6
Cuts at: 1680 1810 1842 2019 2051 3548 3580 3663
Size: 130 32 177 32 1497 32 83
Fragments arranged by size:
1497 910 694 177 130 83 32 32
32 32 20 6 6 6 6
HincII GTy'rAC
Cuts at: 0 273 3246 3663
Size: 273 2973 417
Fragments arranged by size:
2973 417 273
KasI G'GCGC_C
Cuts at: 0 54 141 381 1507 3607 3663
Size: 54 87 240 1126 2100 56
Fragments arranged by size:
2100 1126 240 87 56 54
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 737 1679 1853 2303 2528 3663
Size: 242 495 942 174 450 225 1135
Fragments arranged by size:
1135 942 495 450 242 225 174
MscI TGG'CCA
Cuts at: 0 1330 2300 3663
Size: 1330 970 1363
Fragments arranged by size:
1363 1330 970
MslI CAynn'nnrTG
Cuts at: 0 1324 3253 3277 3472 3484 3663
Size: 1324 1929 24 195 12 179
Fragments arranged by size:
1929 1324 195 179 24 12
MspA1I CmG'CkG
Cuts at: 0 101 318 389 788 1163 1434 2041 2264
Size: 101 217 71 399 375 271 607 223
Cuts at: 2264 2307 2878 2924 2960 3663
Size: 43 571 46 36 703
Fragments arranged by size:
703 607 571 399 375 271 223 217
101 71 46 43 36
NaeI GCC'GGC
Cuts at: 0 73 489 1407 1897 3063 3606 3663
Size: 73 416 918 490 1166 543 57
Fragments arranged by size:
1166 918 543 490 416 73 57
NarI GG'CG_CC
Cuts at: 0 55 142 382 1508 3608 3663
Size: 55 87 240 1126 2100 55
Fragments arranged by size:
2100 1126 240 87 55 55
NcoI C'CATG_G
Cuts at: 0 1835 3663
Size: 1835 1828
NdeI CA'TA_TG
Cuts at: 0 3213 3663
Size: 3213 450
NgoMIV G'CCGG_C
Cuts at: 0 71 487 1405 1895 3061 3604 3663
Size: 71 416 918 490 1166 543 59
Fragments arranged by size:
1166 918 543 490 416 71 59
NspI r_CATG'y
Cuts at: 0 3045 3396 3618 3663
Size: 3045 351 222 45
Fragments arranged by size:
3045 351 222 45
PasI CC'CwG_GG
Cuts at: 0 222 1179 3663
Size: 222 957 2484
Fragments arranged by size:
2484 957 222
PciI A'CATG_T
Cuts at: 0 3041 3663
Size: 3041 622
PflMI CCAn_nnn'nTGG
Cuts at: 0 2016 2682 3504 3663
Size: 2016 666 822 159
Fragments arranged by size:
2016 822 666 159
PfoI T'CCnGG_A
Cuts at: 0 1679 2966 3566 3663
Size: 1679 1287 600 97
Fragments arranged by size:
1679 1287 600 97
PmlI CAC'GTG
Cuts at: 0 3096 3261 3663
Size: 3096 165 402
Fragments arranged by size:
3096 402 165
PpuMI rG'GwC_Cy
Cuts at: 0 1041 2035 2249 2754 2912 3663
Size: 1041 994 214 505 158 751
Fragments arranged by size:
1041 994 751 505 214 158
PshAI GACnn'nnGTC
Cuts at: 0 560 3232 3663
Size: 560 2672 431
Fragments arranged by size:
2672 560 431
PspOMI G'GGCC_C
Cuts at: 0 64 104 111 414 1145 1175 1248 1541
Size: 64 40 7 303 731 30 73 293
Cuts at: 1541 2090 3663
Size: 549 1573
Fragments arranged by size:
1573 731 549 303 293 73 64 40
30 7
PstI C_TGCA'G
Cuts at: 0 2129 2229 2911 3663
Size: 2129 100 682 752
Fragments arranged by size:
2129 752 682 100
PvuII CAG'CTG
Cuts at: 0 1163 1434 2307 2878 3663
Size: 1163 271 873 571 785
Fragments arranged by size:
1163 873 785 571 271
SacI G_AGCT'C
Cuts at: 0 3155 3663
Size: 3155 508
SacII CC_GC'GG
Cuts at: 0 102 390 789 2042 3663
Size: 102 288 399 1253 1621
Fragments arranged by size:
1621 1253 399 288 102
SalI G'TCGA_C
Cuts at: 0 3244 3663
Size: 3244 419
SanDI GG'GwC_CC
Cuts at: 0 2035 3663
Size: 2035 1628
SapI GCTCTTCn'nnn_
Cuts at: 0 1441 3496 3663
Size: 1441 2055 167
Fragments arranged by size:
2055 1441 167
SbfI CC_TGCA'GG
Cuts at: 0 2911 3663
Size: 2911 752
ScaI AGT'ACT
Cuts at: 0 538 3663
Size: 538 3125
SexAI A'CCwGG_T
Cuts at: 0 3313 3620 3663
Size: 3313 307 43
Fragments arranged by size:
3313 307 43
SfcI C'TryA_G
Cuts at: 0 450 2125 2225 2907 3663
Size: 450 1675 100 682 756
Fragments arranged by size:
1675 756 682 450 100
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 286 1356 3663
Size: 286 1070 2307
Fragments arranged by size:
2307 1070 286
SfoI GGC'GCC
Cuts at: 0 56 143 383 1509 3609 3663
Size: 56 87 240 1126 2100 54
Fragments arranged by size:
2100 1126 240 87 56 54
SgrAI Cr'CCGG_yG
Cuts at: 0 3604 3663
Size: 3604 59
SmaI CCC'GGG
Cuts at: 0 60 109 751 778 1971 2746 2856 3173
Size: 60 49 642 27 1193 775 110 317
Cuts at: 3173 3663
Size: 490
Fragments arranged by size:
1193 775 642 490 317 110 60 49
27
SmlI C'TyrA_G
Cuts at: 0 1708 2488 2602 2815 3121 3663
Size: 1708 780 114 213 306 542
Fragments arranged by size:
1708 780 542 306 213 114
SphI G_CATG'C
Cuts at: 0 3618 3663
Size: 3618 45
StuI AGG'CCT
Cuts at: 0 1350 1714 3663
Size: 1350 364 1949
Fragments arranged by size:
1949 1350 364
StyI C'CwwG_G
Cuts at: 0 608 1010 1112 1196 1454 1835 1916 2894
Size: 608 402 102 84 258 381 81 978
Cuts at: 2894 3663
Size: 769
Fragments arranged by size:
978 769 608 402 381 258 102 84
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 699 2818 2858 3181 3663
Size: 699 2119 40 323 482
Fragments arranged by size:
2119 699 482 323 40
TatI w'GTAC_w
Cuts at: 0 536 2774 3038 3663
Size: 536 2238 264 625
Fragments arranged by size:
2238 625 536 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 795 827 2368 2400 2962 2994 3663
Size: 795 32 1541 32 562 32 669
Fragments arranged by size:
1541 795 669 562 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 408 975 2217 3216 3663
Size: 408 567 1242 999 447
Fragments arranged by size:
1242 999 567 447 408
XmaI C'CCGG_G
Cuts at: 0 58 107 749 776 1969 2744 2854 3171
Size: 58 49 642 27 1193 775 110 317
Cuts at: 3171 3663
Size: 492
Fragments arranged by size:
1193 775 642 492 317 110 58 49
27
ZraI GAC'GTC
Cuts at: 0 3372 3663
Size: 3372 291
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlwNI
ApaI ApaLI AscI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AlfI AloI ApoI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BglII BmtI BsaBI BsaWI BsiWI BsmI BspEI BspHI
BsrDI BstBI BstEII BstZ17I ClaI EcoRI EcoRV FseI
FspAI HindIII HpaI KpnI MfeI MluI NheI NotI
NruI NsiI PacI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SgfI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmnI