Restriction map for
pF1KA1119
(Linear) (Six-base) MAPSORT of: KIAA1119.seq Check: 5156 from: 1 to: 5547
KIAA1119.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1176 2435 3381 4669 5547
Size: 1176 1259 946 1288 878
Fragments arranged by size:
1288 1259 1176 946 878
AatII G_ACGT'C
Cuts at: 0 4642 5111 5547
Size: 4642 469 436
Fragments arranged by size:
4642 469 436
AccI GT'mk_AC
Cuts at: 0 1067 5547
Size: 1067 4480
Acc65I G'GTAC_C
Cuts at: 0 692 3200 5547
Size: 692 2508 2347
Fragments arranged by size:
2508 2347 692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1759 2380 2728 2905 3463 3622 3658 4126
Size: 1759 621 348 177 558 159 36 468
Cuts at: 4126 4303 4702 5547
Size: 177 399 845
Fragments arranged by size:
1759 845 621 558 468 399 348 177
177 159 36
AfeI AGC'GCT
Cuts at: 0 4924 5547
Size: 4924 623
AflII C'TTAA_G
Cuts at: 0 4747 5547
Size: 4747 800
AflIII A'CryG_T
Cuts at: 0 4520 5450 5547
Size: 4520 930 97
Fragments arranged by size:
4520 930 97
AgeI A'CCGG_T
Cuts at: 0 2114 5547
Size: 2114 3433
AhdI GACnn_n'nnGTC
Cuts at: 0 364 5547
Size: 364 5183
AleI CACnn'nnGTG
Cuts at: 0 5192 5547
Size: 5192 355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 1241 2989 3086 5146 5547
Size: 190 1051 1748 97 2060 401
Fragments arranged by size:
2060 1748 1051 401 190 97
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5207 5239 5547
Size: 5207 32 308
Fragments arranged by size:
5207 308 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2039 2646 4056 4479 4782 5241 5430 5547
Size: 2039 607 1410 423 303 459 189 117
Fragments arranged by size:
2039 1410 607 459 423 303 189 117
ApaI G_GGCC'C
Cuts at: 0 2484 2985 5547
Size: 2484 501 2562
Fragments arranged by size:
2562 2484 501
ApaLI G'TGCA_C
Cuts at: 0 27 2899 4564 5547
Size: 27 2872 1665 983
Fragments arranged by size:
2872 1665 983 27
ApoI r'AATT_y
Cuts at: 0 817 3133 4177 4752 5324 5353 5530 5547
Size: 817 2316 1044 575 572 29 177 17
Fragments arranged by size:
2316 1044 817 575 572 177 29 17
AseI AT'TA_AT
Cuts at: 0 329 3857 5547
Size: 329 3528 1690
Fragments arranged by size:
3528 1690 329
AvaI C'yCGr_G
Cuts at: 0 2537 3763 4096 5547
Size: 2537 1226 333 1451
Fragments arranged by size:
2537 1451 1226 333
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 294 327 5547
Size: 294 33 5220
Fragments arranged by size:
5220 294 33
BamHI G'GATC_C
Cuts at: 0 41 5547
Size: 41 5506
BanI G'GyrC_C
Cuts at: 0 692 3200 5547
Size: 692 2508 2347
Fragments arranged by size:
2508 2347 692
BanII G_rGCy'C
Cuts at: 0 17 1575 2147 2484 2917 2985 3764 4361
Size: 17 1558 572 337 433 68 779 597
Cuts at: 4361 5547
Size: 1186
Fragments arranged by size:
1558 1186 779 597 572 433 337 68
17
BbsI GAAGACnn'nnnn_
Cuts at: 0 511 741 802 925 3429 5547
Size: 511 230 61 123 2504 2118
Fragments arranged by size:
2504 2118 511 230 123 61
BbvCI CC'TCA_GC
Cuts at: 0 905 3037 3658 5302 5423 5547
Size: 905 2132 621 1644 121 124
Fragments arranged by size:
2132 1644 905 621 124 121
BclI T'GATC_A
Cuts at: 0 1190 5066 5547
Size: 1190 3876 481
Fragments arranged by size:
3876 1190 481
BglI GCCn_nnn'nGGC
Cuts at: 0 659 2488 3916 5547
Size: 659 1829 1428 1631
Fragments arranged by size:
1829 1631 1428 659
BglII A'GATC_T
Cuts at: 0 2234 2999 3582 4802 5547
Size: 2234 765 583 1220 745
Fragments arranged by size:
2234 1220 765 745 583
BlpI GC'TnA_GC
Cuts at: 0 69 1025 2420 2733 4037 4091 5547
Size: 69 956 1395 313 1304 54 1456
Fragments arranged by size:
1456 1395 1304 956 313 69 54
Bme1580I G_kGCm'C
Cuts at: 0 31 2484 2903 2985 4489 4496 4568 5547
Size: 31 2453 419 82 1504 7 72 979
Fragments arranged by size:
2453 1504 979 419 82 72 31 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 1224 1578 5547
Size: 1224 354 3969
Fragments arranged by size:
3969 1224 354
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1695 2748 5547
Size: 1695 1053 2799
Fragments arranged by size:
2799 1695 1053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 301 515 1369 1438 1581 1714 2206
Size: 145 156 214 854 69 143 133 492
Cuts at: 2206 2287 2440 3079 3202 3269 3370 3430 3490
Size: 81 153 639 123 67 101 60 60
Cuts at: 3490 3523 3637 3681 4051 4078 4432 4914 5547
Size: 33 114 44 370 27 354 482 633
Fragments arranged by size:
854 639 633 492 482 370 354 214
156 153 145 143 133 123 114 101
81 69 67 60 60 44 33 27
Bpu10I CC'TnA_GC
Cuts at: 0 229 905 1072 3037 3658 5302 5423 5547
Size: 229 676 167 1965 621 1644 121 124
Fragments arranged by size:
1965 1644 676 621 229 167 124 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 2674 3727 4069 4543 5179 5547
Size: 1267 1407 1053 342 474 636 368
Fragments arranged by size:
1407 1267 1053 636 474 368 342
BsaI GGTCTCn'nnnn_
Cuts at: 0 1150 1927 5547
Size: 1150 777 3620
Fragments arranged by size:
3620 1150 777
BsaAI yAC'GTr
Cuts at: 0 2610 5547
Size: 2610 2937
BsaBI GATnn'nnATC
Cuts at: 0 1732 2062 5547
Size: 1732 330 3485
Fragments arranged by size:
3485 1732 330
BsaHI Gr'CG_yC
Cuts at: 0 3011 4639 5108 5547
Size: 3011 1628 469 439
Fragments arranged by size:
3011 1628 469 439
BsaWI w'CCGG_w
Cuts at: 0 2114 2291 2681 4445 4916 5547
Size: 2114 177 390 1764 471 631
Fragments arranged by size:
2114 1764 631 471 390 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1579 1609 5547
Size: 1579 30 3938
Fragments arranged by size:
3938 1579 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 887 931 2962 3154 3199 3202 3772 3793
Size: 887 44 2031 192 45 3 570 21
Cuts at: 3793 4081 4084 4126 4429 4917 5176 5547
Size: 288 3 42 303 488 259 371
Fragments arranged by size:
2031 887 570 488 371 303 288 259
192 45 44 42 21 3 3
BseYI C'CCAG_C
Cuts at: 0 1509 5234 5309 5511 5547
Size: 1509 3725 75 202 36
Fragments arranged by size:
3725 1509 202 75 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1255 2044 2090 3517 5547
Size: 863 392 789 46 1427 2030
Fragments arranged by size:
2030 1427 863 789 392 46
BsiHKAI G_wGCw'C
Cuts at: 0 17 31 1106 1121 1256 1883 2147 2426
Size: 17 14 1075 15 135 627 264 279
Cuts at: 2426 2456 2585 2903 3764 3788 4361 4568 5125
Size: 30 129 318 861 24 573 207 557
Cuts at: 5125 5547
Size: 422
Fragments arranged by size:
1075 861 627 573 557 422 318 279
264 207 135 129 30 24 17 15
14
BsmI GAATG_Cn'
Cuts at: 0 1947 3582 4880 4992 5547
Size: 1947 1635 1298 112 555
Fragments arranged by size:
1947 1635 1298 555 112
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2047 2758 5547
Size: 2047 711 2789
Fragments arranged by size:
2789 2047 711
Bsp1286I G_dGCh'C
Cuts at: 0 17 31 1106 1121 1256 1575 1883 2147
Size: 17 14 1075 15 135 319 308 264
Cuts at: 2147 2426 2456 2484 2585 2903 2917 2985 3764
Size: 279 30 28 101 318 14 68 779
Cuts at: 3764 3788 4361 4489 4496 4568 5125 5547
Size: 24 573 128 7 72 557 422
Fragments arranged by size:
1075 779 573 557 422 319 318 308
279 264 135 128 101 72 68 30
28 24 17 15 14 14 7
BspEI T'CCGG_A
Cuts at: 0 2291 2681 4445 5547
Size: 2291 390 1764 1102
Fragments arranged by size:
2291 1764 1102 390
BspHI T'CATG_A
Cuts at: 0 240 1776 2306 3167 4709 5547
Size: 240 1536 530 861 1542 838
Fragments arranged by size:
1542 1536 861 838 530 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1176 2165 2435 3381 4359 4669 5031 5099
Size: 1176 989 270 946 978 310 362 68
Cuts at: 5099 5547
Size: 448
Fragments arranged by size:
1176 989 978 946 448 362 310 270
68
BsrBI CCG'CTC
Cuts at: 0 2443 2732 2880 5412 5547
Size: 2443 289 148 2532 135
Fragments arranged by size:
2532 2443 289 148 135
BsrDI GCAATG_nn'
Cuts at: 0 645 5547
Size: 645 4902
BsrFI r'CCGG_y
Cuts at: 0 805 1589 2114 3832 4909 5547
Size: 805 784 525 1718 1077 638
Fragments arranged by size:
1718 1077 805 784 638 525
BsrGI T'GTAC_A
Cuts at: 0 159 5547
Size: 159 5388
BssHII G'CGCG_C
Cuts at: 0 1198 5547
Size: 1198 4349
BssSI C'ACGA_G
Cuts at: 0 3754 5547
Size: 3754 1793
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1082 4572 5547
Size: 1082 3490 975
Fragments arranged by size:
3490 1082 975
BstBI TT'CG_AA
Cuts at: 0 1828 2061 5547
Size: 1828 233 3486
Fragments arranged by size:
3486 1828 233
BstEII G'GTnAC_C
Cuts at: 0 1143 5547
Size: 1143 4404
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2090 4029 5547
Size: 2090 1939 1518
Fragments arranged by size:
2090 1939 1518
BstYI r'GATC_y
Cuts at: 0 41 1445 2234 2999 3582 4802 5547
Size: 41 1404 789 765 583 1220 745
Fragments arranged by size:
1404 1220 789 765 745 583 41
BstZ17I GTA'TAC
Cuts at: 0 1068 5547
Size: 1068 4479
Bsu36I CC'TnA_GG
Cuts at: 0 2716 2935 3793 5547
Size: 2716 219 858 1754
Fragments arranged by size:
2716 1754 858 219
BtgI C'CryG_G
Cuts at: 0 2867 3422 3668 3702 5210 5492 5547
Size: 2867 555 246 34 1508 282 55
Fragments arranged by size:
2867 1508 555 282 246 55 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1117 5547
Size: 1117 4430
BtsI GCAGTG_nn'
Cuts at: 0 2027 4721 5081 5547
Size: 2027 2694 360 466
Fragments arranged by size:
2694 2027 466 360
ClaI AT'CG_AT
Cuts at: 0 2801 5547
Size: 2801 2746
DraI TTT'AAA
Cuts at: 0 822 3971 5330 5547
Size: 822 3149 1359 217
Fragments arranged by size:
3149 1359 822 217
DrdI GACnn_nn'nnGTC
Cuts at: 0 3433 5547
Size: 3433 2114
EaeI y'GGCC_r
Cuts at: 0 384 449 557 1896 2223 3671 5547
Size: 384 65 108 1339 327 1448 1876
Fragments arranged by size:
1876 1448 1339 384 327 108 65
EarI CTCTTCn'nnn_
Cuts at: 0 572 1715 2131 3448 3502 3598 5062 5547
Size: 572 1143 416 1317 54 96 1464 485
Fragments arranged by size:
1464 1317 1143 572 485 416 96 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3312 3556 4966 5547
Size: 3312 244 1410 581
Fragments arranged by size:
3312 1410 581 244
EcoICRI GAG'CTC
Cuts at: 0 15 2145 3762 4359 5547
Size: 15 2130 1617 597 1188
Fragments arranged by size:
2130 1617 1188 597 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 301 515 1369 1438 1581 1714 1759
Size: 145 156 214 854 69 143 133 45
Cuts at: 1759 2206 2287 2380 2440 2728 2905 3079 3202
Size: 447 81 93 60 288 177 174 123
Cuts at: 3202 3269 3370 3430 3463 3490 3523 3622 3637
Size: 67 101 60 33 27 33 99 15
Cuts at: 3637 3658 3681 4051 4078 4126 4303 4432 4702
Size: 21 23 370 27 48 177 129 270
Cuts at: 4702 4914 5547
Size: 212 633
Fragments arranged by size:
854 633 447 370 288 270 214 212
177 177 174 156 145 143 133 129
123 101 99 93 81 69 67 60
60 48 45 33 33 27 27 23
21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1183 2197 2496 3214 3388 3412 4108 5547
Size: 1183 1014 299 718 174 24 696 1439
Fragments arranged by size:
1439 1183 1014 718 696 299 174 24
EcoO109I rG'GnC_Cy
Cuts at: 0 1266 1305 1545 1788 2202 2480 2571 2712
Size: 1266 39 240 243 414 278 91 141
Cuts at: 2712 2981 3797 4101 4308 4434 5547
Size: 269 816 304 207 126 1113
Fragments arranged by size:
1266 1113 816 414 304 278 269 243
240 207 141 126 91 39
EcoRI G'AATT_C
Cuts at: 0 4177 5530 5547
Size: 4177 1353 17
Fragments arranged by size:
4177 1353 17
EcoRV GAT'ATC
Cuts at: 0 198 5547
Size: 198 5349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2013 2256 5547
Size: 2013 243 3291
Fragments arranged by size:
3291 2013 243
FspI TGC'GCA
Cuts at: 0 2965 3772 5547
Size: 2965 807 1775
Fragments arranged by size:
2965 1775 807
HaeII r_GCGC'y
Cuts at: 0 69 3815 4353 4926 5547
Size: 69 3746 538 573 621
Fragments arranged by size:
3746 621 573 538 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 350 382 1579 1611 2827 2859 3178 3210
Size: 350 32 1197 32 1216 32 319 32
Cuts at: 3210 3604 3636 4624 4656 5098 5130 5547
Size: 394 32 988 32 442 32 417
Fragments arranged by size:
1216 1197 988 442 417 394 350 319
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 80 3897 5547
Size: 80 3817 1650
Fragments arranged by size:
3817 1650 80
HpaI GTT'AAC
Cuts at: 0 80 5547
Size: 80 5467
KpnI G_GTAC'C
Cuts at: 0 696 3204 5547
Size: 696 2508 2343
Fragments arranged by size:
2508 2343 696
MfeI C'AATT_G
Cuts at: 0 152 5547
Size: 152 5395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1508 1655 1895 2030 3212 4679 4847
Size: 311 1197 147 240 135 1182 1467 168
Cuts at: 4847 5547
Size: 700
Fragments arranged by size:
1467 1197 1182 700 311 240 168 147
135
MscI TGG'CCA
Cuts at: 0 386 451 1898 5547
Size: 386 65 1447 3649
Fragments arranged by size:
3649 1447 386 65
MslI CAynn'nnrTG
Cuts at: 0 1223 2088 2305 2548 3427 4622 5128 5192
Size: 1223 865 217 243 879 1195 506 64
Cuts at: 5192 5547
Size: 355
Fragments arranged by size:
1223 1195 879 865 506 355 243 217
64
MspA1I CmG'CkG
Cuts at: 0 73 174 1967 2074 2643 2787 2793 3501
Size: 73 101 1793 107 569 144 6 708
Cuts at: 3501 3822 4041 4383 4694 5547
Size: 321 219 342 311 853
Fragments arranged by size:
1793 853 708 569 342 321 311 219
144 107 101 73 6
NaeI GCC'GGC
Cuts at: 0 3834 5547
Size: 3834 1713
NcoI C'CATG_G
Cuts at: 0 2867 3422 5210 5492 5547
Size: 2867 555 1788 282 55
Fragments arranged by size:
2867 1788 555 282 55
NgoMIV G'CCGG_C
Cuts at: 0 3832 5547
Size: 3832 1715
NspI r_CATG'y
Cuts at: 0 1408 1629 2475 3895 4410 4524 5133 5454
Size: 1408 221 846 1420 515 114 609 321
Cuts at: 5454 5547
Size: 93
Fragments arranged by size:
1420 1408 846 609 515 321 221 114
93
PasI CC'CwG_GG
Cuts at: 0 1810 5547
Size: 1810 3737
PciI A'CATG_T
Cuts at: 0 4520 5450 5547
Size: 4520 930 97
Fragments arranged by size:
4520 930 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 355 393 3393 4056 4329 5019 5547
Size: 355 38 3000 663 273 690 528
Fragments arranged by size:
3000 690 663 528 355 273 38
PfoI T'CCnGG_A
Cuts at: 0 278 1307 2183 5547
Size: 278 1029 876 3364
Fragments arranged by size:
3364 1029 876 278
PmlI CAC'GTG
Cuts at: 0 2610 5547
Size: 2610 2937
PpuMI rG'GwC_Cy
Cuts at: 0 1305 1545 1788 2202 2571 3797 4308 5547
Size: 1305 240 243 414 369 1226 511 1239
Fragments arranged by size:
1305 1239 1226 511 414 369 243 240
PshAI GACnn'nnGTC
Cuts at: 0 1162 5547
Size: 1162 4385
PspOMI G'GGCC_C
Cuts at: 0 2480 2981 5547
Size: 2480 501 2566
Fragments arranged by size:
2566 2480 501
PspXI vC'TCGA_Gb
Cuts at: 0 3763 4096 5547
Size: 3763 333 1451
Fragments arranged by size:
3763 1451 333
PstI C_TGCA'G
Cuts at: 0 252 1184 2176 2339 2384 2792 2945 2978
Size: 252 932 992 163 45 408 153 33
Cuts at: 2978 3452 4046 5042 5547
Size: 474 594 996 505
Fragments arranged by size:
996 992 932 594 505 474 408 252
163 153 45 33
PvuII CAG'CTG
Cuts at: 0 73 174 2074 2787 3501 4041 5547
Size: 73 101 1900 713 714 540 1506
Fragments arranged by size:
1900 1506 714 713 540 101 73
SacI G_AGCT'C
Cuts at: 0 17 2147 3764 4361 5547
Size: 17 2130 1617 597 1186
Fragments arranged by size:
2130 1617 1186 597 17
SapI GCTCTTCn'nnn_
Cuts at: 0 1715 5062 5547
Size: 1715 3347 485
Fragments arranged by size:
3347 1715 485
SbfI CC_TGCA'GG
Cuts at: 0 5042 5547
Size: 5042 505
SexAI A'CCwGG_T
Cuts at: 0 4451 5547
Size: 4451 1096
SfcI C'TryA_G
Cuts at: 0 18 112 248 378 1044 1180 2172 2335
Size: 18 94 136 130 666 136 992 163
Cuts at: 2335 2380 2788 2941 2974 3448 3720 4042 5038
Size: 45 408 153 33 474 272 322 996
Cuts at: 5038 5547
Size: 509
Fragments arranged by size:
996 992 666 509 474 408 322 272
163 153 136 136 130 94 45 33
18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2488 5547
Size: 2488 3059
SmaI CCC'GGG
Cuts at: 0 2539 5547
Size: 2539 3008
SmlI C'TyrA_G
Cuts at: 0 1282 2689 3742 3763 4084 4096 4558 4747
Size: 1282 1407 1053 21 321 12 462 189
Cuts at: 4747 5158 5547
Size: 411 389
Fragments arranged by size:
1407 1282 1053 462 411 389 321 189
21 12
SpeI A'CTAG_T
Cuts at: 0 1758 5547
Size: 1758 3789
SphI G_CATG'C
Cuts at: 0 2475 4410 5133 5547
Size: 2475 1935 723 414
Fragments arranged by size:
2475 1935 723 414
SspI AAT'ATT
Cuts at: 0 3223 5547
Size: 3223 2324
StuI AGG'CCT
Cuts at: 0 2491 2530 3919 4007 5547
Size: 2491 39 1389 88 1540
Fragments arranged by size:
2491 1540 1389 88 39
StyI C'CwwG_G
Cuts at: 0 83 1002 1450 2867 3422 4002 5210 5492
Size: 83 919 448 1417 555 580 1208 282
Cuts at: 5492 5547
Size: 55
Fragments arranged by size:
1417 1208 919 580 555 448 282 83
55
SwaI ATTT'AAAT
Cuts at: 0 3971 5547
Size: 3971 1576
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 5323 5547
Size: 5323 224
TatI w'GTAC_w
Cuts at: 0 159 665 4688 5547
Size: 159 506 4023 859
Fragments arranged by size:
4023 859 506 159
Tth111I GACn'n_nGTC
Cuts at: 0 1173 5547
Size: 1173 4374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 603 891 4218 5547
Size: 603 288 3327 1329
Fragments arranged by size:
3327 1329 603 288
XhoI C'TCGA_G
Cuts at: 0 3763 4096 5547
Size: 3763 333 1451
Fragments arranged by size:
3763 1451 333
XmaI C'CCGG_G
Cuts at: 0 2537 5547
Size: 2537 3010
XmnI GAAnn'nnTTC
Cuts at: 0 137 4396 5547
Size: 137 4259 1151
Fragments arranged by size:
4259 1151 137
ZraI GAC'GTC
Cuts at: 0 4640 5109 5547
Size: 4640 469 438
Fragments arranged by size:
4640 469 438
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KpnI MfeI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SapI SbfI SexAI SfcI SfiI
SmaI SmlI SpeI SphI SspI StuI StyI SwaI
TaqII TatI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AscI AsiSI AvrII BbeI BcgI BciVI BfrBI
BmgBI BmtI BsiEI BsiWI CspCI DraIII EagI FseI
FspAI HindIII KasI MluI NarI NdeI NheI NotI
NruI NsiI PacI PmeI PpiI PsiI PsrI PvuI
RsrII SacII SalI SanDI ScaI SfoI SgfI SgrAI
SnaBI SrfI TstI XbaI