Restriction map for
pF1KSDA1089
(Linear) (Six-base) MAPSORT of: KIAA1089.seq Check: 5672 from: 1 to: 3048
KIAA1089.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2872 3048
Size: 2872 176
AccI GT'mk_AC
Cuts at: 0 257 1835 3048
Size: 257 1578 1213
Fragments arranged by size:
1578 1213 257
Acc65I G'GTAC_C
Cuts at: 0 2720 3048
Size: 2720 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1829 1921 2482 2704 2824 3048
Size: 817 1012 92 561 222 120 224
Fragments arranged by size:
1012 817 561 224 222 120 92
AfeI AGC'GCT
Cuts at: 0 1735 3048
Size: 1735 1313
AleI CACnn'nnGTG
Cuts at: 0 2993 3048
Size: 2993 55
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 362 1148 2365 2999 3048
Size: 362 786 1217 634 49
Fragments arranged by size:
1217 786 634 362 49
AlwNI CAG_nnn'CTG
Cuts at: 0 472 1303 1858 1944 3048
Size: 472 831 555 86 1104
Fragments arranged by size:
1104 831 555 472 86
ApaI G_GGCC'C
Cuts at: 0 1672 2706 3048
Size: 1672 1034 342
Fragments arranged by size:
1672 1034 342
ApaLI G'TGCA_C
Cuts at: 0 1096 3048
Size: 1096 1952
AvaI C'yCGr_G
Cuts at: 0 1460 1625 2705 3048
Size: 1460 165 1080 343
Fragments arranged by size:
1460 1080 343 165
BamHI G'GATC_C
Cuts at: 0 1117 2892 3048
Size: 1117 1775 156
Fragments arranged by size:
1775 1117 156
BanI G'GyrC_C
Cuts at: 0 655 2720 3048
Size: 655 2065 328
Fragments arranged by size:
2065 655 328
BanII G_rGCy'C
Cuts at: 0 38 185 1477 1622 1672 1870 2035 2706
Size: 38 147 1292 145 50 198 165 671
Cuts at: 2706 3048
Size: 342
Fragments arranged by size:
1292 671 342 198 165 147 145 50
38
BbsI GAAGACnn'nnnn_
Cuts at: 0 1971 3048
Size: 1971 1077
BbvCI CC'TCA_GC
Cuts at: 0 233 997 3048
Size: 233 764 2051
Fragments arranged by size:
2051 764 233
BglI GCCn_nnn'nGGC
Cuts at: 0 1667 2339 2878 2977 3048
Size: 1667 672 539 99 71
Fragments arranged by size:
1667 672 539 99 71
BglII A'GATC_T
Cuts at: 0 2133 3048
Size: 2133 915
BlpI GC'TnA_GC
Cuts at: 0 1202 2266 2486 3048
Size: 1202 1064 220 562
Fragments arranged by size:
1202 1064 562 220
Bme1580I G_kGCm'C
Cuts at: 0 501 658 1100 1672 2706 3048
Size: 501 157 442 572 1034 342
Fragments arranged by size:
1034 572 501 442 342 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 205 1667 1675 2385 2541 3048
Size: 205 1462 8 710 156 507
Fragments arranged by size:
1462 710 507 205 156 8
BmtI G_CTAG'C
Cuts at: 0 589 1685 3048
Size: 589 1096 1363
Fragments arranged by size:
1363 1096 589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 945 1872 1884 3048
Size: 945 927 12 1164
Fragments arranged by size:
1164 945 927 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1636 1759 2080 2539 2746 3048
Size: 970 666 123 321 459 207 302
Fragments arranged by size:
970 666 459 321 302 207 123
Bpu10I CC'TnA_GC
Cuts at: 0 233 997 1304 3048
Size: 233 764 307 1744
Fragments arranged by size:
1744 764 307 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 1428 3048
Size: 739 689 1620
Fragments arranged by size:
1620 739 689
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 967 997 2719 2749 3048
Size: 229 30 708 30 1722 30 299
Fragments arranged by size:
1722 708 299 229 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 614 975 1033 1036 1301 1303 1306
Size: 250 364 361 58 3 265 2 3
Cuts at: 1306 1606 1762 1876 2293 2902 3048
Size: 300 156 114 417 609 146
Fragments arranged by size:
609 417 364 361 300 265 250 156
146 114 58 3 3 2
BseYI C'CCAG_C
Cuts at: 0 651 2941 3048
Size: 651 2290 107
Fragments arranged by size:
2290 651 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1154 3048
Size: 190 964 1894
Fragments arranged by size:
1894 964 190
BsiHKAI G_wGCw'C
Cuts at: 0 185 316 1100 2035 3048
Size: 185 131 784 935 1013
Fragments arranged by size:
1013 935 784 185 131
BsmI GAATG_Cn'
Cuts at: 0 641 1234 3048
Size: 641 593 1814
Fragments arranged by size:
1814 641 593
Bsp1286I G_dGCh'C
Cuts at: 0 38 185 316 501 658 1100 1477 1622
Size: 38 147 131 185 157 442 377 145
Cuts at: 1622 1672 1870 2035 2706 3048
Size: 50 198 165 671 342
Fragments arranged by size:
671 442 377 342 198 185 165 157
147 145 131 50 38
BspHI T'CATG_A
Cuts at: 0 2471 3048
Size: 2471 577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 713 986 1775 2216 2872 3048
Size: 713 273 789 441 656 176
Fragments arranged by size:
789 713 656 441 273 176
BsrBI CCG'CTC
Cuts at: 0 2217 2793 3048
Size: 2217 576 255
Fragments arranged by size:
2217 576 255
BsrDI GCAATG_nn'
Cuts at: 0 1139 1685 2384 3048
Size: 1139 546 699 664
Fragments arranged by size:
1139 699 664 546
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1141 2300 3048
Size: 1141 1159 748
Fragments arranged by size:
1159 1141 748
BstEII G'GTnAC_C
Cuts at: 0 1604 2907 3048
Size: 1604 1303 141
Fragments arranged by size:
1604 1303 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1246 2104 3048
Size: 1246 858 944
Fragments arranged by size:
1246 944 858
BstYI r'GATC_y
Cuts at: 0 1117 1299 2133 2840 2892 3048
Size: 1117 182 834 707 52 156
Fragments arranged by size:
1117 834 707 182 156 52
BstZ17I GTA'TAC
Cuts at: 0 258 3048
Size: 258 2790
Bsu36I CC'TnA_GG
Cuts at: 0 163 477 2123 3048
Size: 163 314 1646 925
Fragments arranged by size:
1646 925 314 163
BtgI C'CryG_G
Cuts at: 0 911 3048
Size: 911 2137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 852 2310 3048
Size: 852 1458 738
Fragments arranged by size:
1458 852 738
BtsI GCAGTG_nn'
Cuts at: 0 418 3037 3048
Size: 418 2619 11
Fragments arranged by size:
2619 418 11
ClaI AT'CG_AT
Cuts at: 0 2666 3048
Size: 2666 382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1798 1833 2026 2061 3048
Size: 1798 35 193 35 987
Fragments arranged by size:
1798 987 193 35 35
DraI TTT'AAA
Cuts at: 0 2739 3048
Size: 2739 309
DraIII CAC_nnn'GTG
Cuts at: 0 213 3048
Size: 213 2835
DrdI GACnn_nn'nnGTC
Cuts at: 0 1904 3048
Size: 1904 1144
EaeI y'GGCC_r
Cuts at: 0 1004 1802 1949 2621 2631 3048
Size: 1004 798 147 672 10 417
Fragments arranged by size:
1004 798 672 417 147 10
EarI CTCTTCn'nnn_
Cuts at: 0 976 1205 1583 2204 2800 3048
Size: 976 229 378 621 596 248
Fragments arranged by size:
976 621 596 378 248 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1366 2602 3048
Size: 1366 1236 446
Fragments arranged by size:
1366 1236 446
EcoICRI GAG'CTC
Cuts at: 0 183 2033 3048
Size: 183 1850 1015
Fragments arranged by size:
1850 1015 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 970 1636 1759 1829 1921 2080 2482
Size: 817 153 666 123 70 92 159 402
Cuts at: 2482 2539 2704 2746 2824 3048
Size: 57 165 42 78 224
Fragments arranged by size:
817 666 402 224 165 159 153 123
92 78 70 57 42
EcoO109I rG'GnC_Cy
Cuts at: 0 10 1669 1941 2586 2702 2956 3048
Size: 10 1659 272 645 116 254 92
Fragments arranged by size:
1659 645 272 254 116 92 10
EcoRV GAT'ATC
Cuts at: 0 550 3048
Size: 550 2498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1755 1978 2155 3048
Size: 1755 223 177 893
Fragments arranged by size:
1755 893 223 177
FspI TGC'GCA
Cuts at: 0 2027 2293 3048
Size: 2027 266 755
Fragments arranged by size:
2027 755 266
FspAI rTGC'GCAy
Cuts at: 0 2293 3048
Size: 2293 755
HaeII r_GCGC'y
Cuts at: 0 1737 3048
Size: 1737 1311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 89 121 495 527 1471 1503 1549 1581
Size: 89 32 374 32 944 32 46 32
Cuts at: 1581 2152 2184 2486 2518 3048
Size: 571 32 302 32 530
Fragments arranged by size:
944 571 530 374 302 89 46 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1185 3048
Size: 1185 1863
HindIII A'AGCT_T
Cuts at: 0 193 1933 3048
Size: 193 1740 1115
Fragments arranged by size:
1740 1115 193
KpnI G_GTAC'C
Cuts at: 0 2724 3048
Size: 2724 324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1767 1856 1888 2440 3048
Size: 1767 89 32 552 608
Fragments arranged by size:
1767 608 552 89 32
MscI TGG'CCA
Cuts at: 0 1006 2623 3048
Size: 1006 1617 425
Fragments arranged by size:
1617 1006 425
MslI CAynn'nnrTG
Cuts at: 0 1717 2017 2993 3048
Size: 1717 300 976 55
Fragments arranged by size:
1717 976 300 55
MspA1I CmG'CkG
Cuts at: 0 402 651 2517 2852 2862 2983 3048
Size: 402 249 1866 335 10 121 65
Fragments arranged by size:
1866 402 335 249 121 65 10
NheI G'CTAG_C
Cuts at: 0 585 1681 3048
Size: 585 1096 1367
Fragments arranged by size:
1367 1096 585
NspI r_CATG'y
Cuts at: 0 512 3048
Size: 512 2536
PasI CC'CwG_GG
Cuts at: 0 2698 2874 3048
Size: 2698 176 174
Fragments arranged by size:
2698 176 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 2368 3048
Size: 2368 680
PfoI T'CCnGG_A
Cuts at: 0 947 1049 1925 1982 2096 3048
Size: 947 102 876 57 114 952
Fragments arranged by size:
952 947 876 114 102 57
PpuMI rG'GwC_Cy
Cuts at: 0 1941 3048
Size: 1941 1107
PshAI GACnn'nnGTC
Cuts at: 0 433 1496 3048
Size: 433 1063 1552
Fragments arranged by size:
1552 1063 433
PspOMI G'GGCC_C
Cuts at: 0 1668 2702 3048
Size: 1668 1034 346
Fragments arranged by size:
1668 1034 346
PstI C_TGCA'G
Cuts at: 0 413 1199 2354 2417 2534 2978 3048
Size: 413 786 1155 63 117 444 70
Fragments arranged by size:
1155 786 444 413 117 70 63
PvuII CAG'CTG
Cuts at: 0 402 651 2517 2852 2862 3048
Size: 402 249 1866 335 10 186
Fragments arranged by size:
1866 402 335 249 186 10
SacI G_AGCT'C
Cuts at: 0 185 2035 3048
Size: 185 1850 1013
Fragments arranged by size:
1850 1013 185
SapI GCTCTTCn'nnn_
Cuts at: 0 2204 3048
Size: 2204 844
SbfI CC_TGCA'GG
Cuts at: 0 2978 3048
Size: 2978 70
ScaI AGT'ACT
Cuts at: 0 676 3048
Size: 676 2372
SexAI A'CCwGG_T
Cuts at: 0 341 3048
Size: 341 2707
SfcI C'TryA_G
Cuts at: 0 330 409 892 1195 2350 2413 2530 2974
Size: 330 79 483 303 1155 63 117 444
Cuts at: 2974 3048
Size: 74
Fragments arranged by size:
1155 483 444 330 303 117 79 74
63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1667 3048
Size: 1667 1381
SmaI CCC'GGG
Cuts at: 0 1462 2707 3048
Size: 1462 1245 341
Fragments arranged by size:
1462 1245 341
SmlI C'TyrA_G
Cuts at: 0 754 1443 3048
Size: 754 689 1605
Fragments arranged by size:
1605 754 689
SrfI GCCC'GGGC
Cuts at: 0 1462 3048
Size: 1462 1586
StuI AGG'CCT
Cuts at: 0 1815 2924 3048
Size: 1815 1109 124
Fragments arranged by size:
1815 1109 124
StyI C'CwwG_G
Cuts at: 0 6 1268 2540 2653 3048
Size: 6 1262 1272 113 395
Fragments arranged by size:
1272 1262 395 113 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 224 1005 1321 1406 1410 1729 2596 2711
Size: 224 781 316 85 4 319 867 115
Cuts at: 2711 3048
Size: 337
Fragments arranged by size:
867 781 337 319 316 224 115 85
4
TatI w'GTAC_w
Cuts at: 0 674 1127 3048
Size: 674 453 1921
Fragments arranged by size:
1921 674 453
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 397 429 2910 2942 3048
Size: 229 32 136 32 2481 32 106
Fragments arranged by size:
2481 229 136 106 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1602 3048
Size: 1602 1446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 999 1275 3048
Size: 999 276 1773
Fragments arranged by size:
1773 999 276
XmaI C'CCGG_G
Cuts at: 0 1460 2705 3048
Size: 1460 1245 343
Fragments arranged by size:
1460 1245 343
XmnI GAAnn'nnTTC
Cuts at: 0 1978 3048
Size: 1978 1070
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV FalI FspI FspAI HaeII Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NheI NspI
PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI SapI SbfI ScaI SexAI SfcI SfiI SmaI
SmlI SrfI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BmgBI BsaI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BssSI
BstBI EagI EcoNI EcoRI FseI HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SfoI SgfI
SgrAI SnaBI SpeI SphI SspI SwaI XbaI XhoI
ZraI