Restriction map for
pF1KA1079
(Linear) (Six-base) MAPSORT of: KIAA1079.seq Check: 4484 from: 1 to: 4362
KIAA1079.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 123 3558 3995 4193 4362
Size: 123 3435 437 198 169
Fragments arranged by size:
3435 437 198 169 123
AccI GT'mk_AC
Cuts at: 0 1259 4091 4362
Size: 1259 2832 271
Fragments arranged by size:
2832 1259 271
Acc65I G'GTAC_C
Cuts at: 0 2442 2484 4362
Size: 2442 42 1878
Fragments arranged by size:
2442 1878 42
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 577 685 1220 1306 2218 2285 2296
Size: 559 18 108 535 86 912 67 11
Cuts at: 2296 4362
Size: 2066
Fragments arranged by size:
2066 912 559 535 108 86 67 18
11
AfeI AGC'GCT
Cuts at: 0 1709 3023 4362
Size: 1709 1314 1339
Fragments arranged by size:
1709 1339 1314
AflII C'TTAA_G
Cuts at: 0 2245 4362
Size: 2245 2117
AflIII A'CryG_T
Cuts at: 0 1866 2527 4362
Size: 1866 661 1835
Fragments arranged by size:
1866 1835 661
AhdI GACnn_n'nnGTC
Cuts at: 0 2946 4362
Size: 2946 1416
AleI CACnn'nnGTG
Cuts at: 0 3256 4362
Size: 3256 1106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 113 2434 3452 4362
Size: 113 2321 1018 910
Fragments arranged by size:
2321 1018 910 113
AlwNI CAG_nnn'CTG
Cuts at: 0 1413 1549 2584 2968 3602 4362
Size: 1413 136 1035 384 634 760
Fragments arranged by size:
1413 1035 760 634 384 136
ApaI G_GGCC'C
Cuts at: 0 1556 3760 4133 4362
Size: 1556 2204 373 229
Fragments arranged by size:
2204 1556 373 229
ApaLI G'TGCA_C
Cuts at: 0 1207 4362
Size: 1207 3155
ApoI r'AATT_y
Cuts at: 0 232 2603 4362
Size: 232 2371 1759
Fragments arranged by size:
2371 1759 232
AseI AT'TA_AT
Cuts at: 0 172 4362
Size: 172 4190
AvaI C'yCGr_G
Cuts at: 0 1882 3118 3142 3337 3360 3409 3424 3805
Size: 1882 1236 24 195 23 49 15 381
Cuts at: 3805 4036 4362
Size: 231 326
Fragments arranged by size:
1882 1236 381 326 231 195 49 24
23 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2369 2402 4362
Size: 2369 33 1960
Fragments arranged by size:
2369 1960 33
BanI G'GyrC_C
Cuts at: 0 108 2442 2484 3148 3983 4362
Size: 108 2334 42 664 835 379
Fragments arranged by size:
2334 835 664 379 108 42
BanII G_rGCy'C
Cuts at: 0 1556 1891 3338 3760 4133 4362
Size: 1556 335 1447 422 373 229
Fragments arranged by size:
1556 1447 422 373 335 229
BbeI G_GCGC'C
Cuts at: 0 112 4362
Size: 112 4250
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 306 2417 2801 3590 4362
Size: 287 19 2111 384 789 772
Fragments arranged by size:
2111 789 772 384 287 19
BbvCI CC'TCA_GC
Cuts at: 0 3916 3964 4362
Size: 3916 48 398
Fragments arranged by size:
3916 398 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1330 1364 3688 3722 4362
Size: 1330 34 2324 34 640
Fragments arranged by size:
2324 1330 640 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 207 4362
Size: 207 4155
BlpI GC'TnA_GC
Cuts at: 0 1442 4309 4362
Size: 1442 2867 53
Fragments arranged by size:
2867 1442 53
Bme1580I G_kGCm'C
Cuts at: 0 478 1211 1556 3068 3151 3760 3956 4133
Size: 478 733 345 1512 83 609 196 177
Cuts at: 4133 4362
Size: 229
Fragments arranged by size:
1512 733 609 478 345 229 196 177
83
BmrI ACTGGGnnnn_n'
Cuts at: 0 1931 2770 4362
Size: 1931 839 1592
Fragments arranged by size:
1931 1592 839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1144 1622 2956 3019 3495 3624 4362
Size: 914 230 478 1334 63 476 129 738
Fragments arranged by size:
1334 914 738 478 476 230 129 63
Bpu10I CC'TnA_GC
Cuts at: 0 1414 1774 3916 3964 4162 4198 4362
Size: 1414 360 2142 48 198 36 164
Fragments arranged by size:
2142 1414 360 198 164 48 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1810 4362
Size: 374 1436 2552
Fragments arranged by size:
2552 1436 374
BsaI GGTCTCn'nnnn_
Cuts at: 0 1004 1182 2668 3931 4102 4362
Size: 1004 178 1486 1263 171 260
Fragments arranged by size:
1486 1263 1004 260 178 171
BsaAI yAC'GTr
Cuts at: 0 1869 2528 4362
Size: 1869 659 1834
Fragments arranged by size:
1869 1834 659
BsaHI Gr'CG_yC
Cuts at: 0 109 4362
Size: 109 4253
BsaWI w'CCGG_w
Cuts at: 0 1515 4362
Size: 1515 2847
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1302 1807 2686 2895 3367 3871 4154 4362
Size: 1302 505 879 209 472 504 283 208
Fragments arranged by size:
1302 879 505 504 472 283 209 208
BseYI C'CCAG_C
Cuts at: 0 70 303 345 1118 1362 1760 2519 2961
Size: 70 233 42 773 244 398 759 442
Cuts at: 2961 3563 4125 4170 4176 4362
Size: 602 562 45 6 186
Fragments arranged by size:
773 759 602 562 442 398 244 233
186 70 45 42 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 177 760 769 2458 3651 4362
Size: 119 58 583 9 1689 1193 711
Fragments arranged by size:
1689 1193 711 583 119 58 9
BsiEI CG_ry'CG
Cuts at: 0 3660 4362
Size: 3660 702
BsiHKAI G_wGCw'C
Cuts at: 0 695 1211 2413 3947 4362
Size: 695 516 1202 1534 415
Fragments arranged by size:
1534 1202 695 516 415
BsmI GAATG_Cn'
Cuts at: 0 1327 2256 3621 4362
Size: 1327 929 1365 741
Fragments arranged by size:
1365 1327 929 741
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3262 4362
Size: 3262 1100
Bsp1286I G_dGCh'C
Cuts at: 0 478 695 1211 1556 1891 2413 3068 3151
Size: 478 217 516 345 335 522 655 83
Cuts at: 3151 3338 3760 3947 3956 4133 4362
Size: 187 422 187 9 177 229
Fragments arranged by size:
655 522 516 478 422 345 335 229
217 187 187 177 83 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 123 857 1235 2004 2430 3558 3899 3914
Size: 123 734 378 769 426 1128 341 15
Cuts at: 3914 3995 4193 4313 4362
Size: 81 198 120 49
Fragments arranged by size:
1128 769 734 426 378 341 198 123
120 81 49 15
BsrBI CCG'CTC
Cuts at: 0 3139 4166 4362
Size: 3139 1027 196
Fragments arranged by size:
3139 1027 196
BsrDI GCAATG_nn'
Cuts at: 0 2416 4336 4362
Size: 2416 1920 26
Fragments arranged by size:
2416 1920 26
BsrFI r'CCGG_y
Cuts at: 0 12 61 1358 2813 3078 3232 4362
Size: 12 49 1297 1455 265 154 1130
Fragments arranged by size:
1455 1297 1130 265 154 49 12
BssSI C'ACGA_G
Cuts at: 0 1500 4362
Size: 1500 2862
BstAPI GCAn_nnn'nTGC
Cuts at: 0 774 783 1215 3951 4362
Size: 774 9 432 2736 411
Fragments arranged by size:
2736 774 432 411 9
BstBI TT'CG_AA
Cuts at: 0 2459 4362
Size: 2459 1903
BstEII G'GTnAC_C
Cuts at: 0 658 4362
Size: 658 3704
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3519 4125 4362
Size: 3519 606 237
Fragments arranged by size:
3519 606 237
BstYI r'GATC_y
Cuts at: 0 1545 3040 4275 4362
Size: 1545 1495 1235 87
Fragments arranged by size:
1545 1495 1235 87
Bsu36I CC'TnA_GG
Cuts at: 0 1591 4182 4362
Size: 1591 2591 180
Fragments arranged by size:
2591 1591 180
BtgI C'CryG_G
Cuts at: 0 749 1193 3173 3578 3680 4362
Size: 749 444 1980 405 102 682
Fragments arranged by size:
1980 749 682 444 405 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 933 2794 3194 3251 3650 4362
Size: 933 1861 400 57 399 712
Fragments arranged by size:
1861 933 712 400 399 57
BtsI GCAGTG_nn'
Cuts at: 0 72 1279 4030 4362
Size: 72 1207 2751 332
Fragments arranged by size:
2751 1207 332 72
DraI TTT'AAA
Cuts at: 0 1992 2849 4362
Size: 1992 857 1513
Fragments arranged by size:
1992 1513 857
DraIII CAC_nnn'GTG
Cuts at: 0 3951 4362
Size: 3951 411
DrdI GACnn_nn'nnGTC
Cuts at: 0 1255 4362
Size: 1255 3107
EaeI y'GGCC_r
Cuts at: 0 758 1575 3186 4013 4362
Size: 758 817 1611 827 349
Fragments arranged by size:
1611 827 817 758 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 39 3366 4362
Size: 39 3327 996
Fragments arranged by size:
3327 996 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 577 685 914 1144 1220 1306 1622
Size: 559 18 108 229 230 76 86 316
Cuts at: 1622 2218 2285 2296 2956 3019 3495 3624 4362
Size: 596 67 11 660 63 476 129 738
Fragments arranged by size:
738 660 596 559 476 316 230 229
129 108 86 76 67 63 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2935 3832 4362
Size: 2935 897 530
Fragments arranged by size:
2935 897 530
EcoO109I rG'GnC_Cy
Cuts at: 0 210 1410 1596 2645 3357 3756 3888 4129
Size: 210 1200 186 1049 712 399 132 241
Cuts at: 4129 4130 4362
Size: 1 232
Fragments arranged by size:
1200 1049 712 399 241 232 210 186
132 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 471 795 4362
Size: 471 324 3567
Fragments arranged by size:
3567 471 324
FspI TGC'GCA
Cuts at: 0 679 4362
Size: 679 3683
HaeII r_GCGC'y
Cuts at: 0 112 1711 1774 3025 4362
Size: 112 1599 63 1251 1337
Fragments arranged by size:
1599 1337 1251 112 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 2629 2661 2835 2867 2932 2964
Size: 274 32 2323 32 174 32 65 32
Cuts at: 2964 3117 3149 3313 3331 3345 3363 4252 4284
Size: 153 32 164 18 14 18 889 32
Cuts at: 4284 4362
Size: 78
Fragments arranged by size:
2323 889 274 174 164 153 78 65
32 32 32 32 32 32 18 18
14
HincII GTy'rAC
Cuts at: 0 1260 4334 4362
Size: 1260 3074 28
Fragments arranged by size:
3074 1260 28
KasI G'GCGC_C
Cuts at: 0 108 4362
Size: 108 4254
KpnI G_GTAC'C
Cuts at: 0 2446 2488 4362
Size: 2446 42 1874
Fragments arranged by size:
2446 1874 42
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 848 1085 1340 1620 3908 4362
Size: 582 266 237 255 280 2288 454
Fragments arranged by size:
2288 582 454 280 266 255 237
MslI CAynn'nnrTG
Cuts at: 0 2225 3256 4362
Size: 2225 1031 1106
Fragments arranged by size:
2225 1106 1031
MspA1I CmG'CkG
Cuts at: 0 751 1122 1195 1245 1574 3194 3280 3632
Size: 751 371 73 50 329 1620 86 352
Cuts at: 3632 4012 4362
Size: 380 350
Fragments arranged by size:
1620 751 380 371 352 350 329 86
73 50
NaeI GCC'GGC
Cuts at: 0 14 63 2815 3080 3234 4362
Size: 14 49 2752 265 154 1128
Fragments arranged by size:
2752 1128 265 154 49 14
NarI GG'CG_CC
Cuts at: 0 109 4362
Size: 109 4253
NgoMIV G'CCGG_C
Cuts at: 0 12 61 2813 3078 3232 4362
Size: 12 49 2752 265 154 1130
Fragments arranged by size:
2752 1130 265 154 49 12
NspI r_CATG'y
Cuts at: 0 699 2340 4362
Size: 699 1641 2022
Fragments arranged by size:
2022 1641 699
PasI CC'CwG_GG
Cuts at: 0 3288 4362
Size: 3288 1074
PflMI CCAn_nnn'nTGG
Cuts at: 0 2634 4362
Size: 2634 1728
PfoI T'CCnGG_A
Cuts at: 0 2546 2753 2933 4362
Size: 2546 207 180 1429
Fragments arranged by size:
2546 1429 207 180
PmlI CAC'GTG
Cuts at: 0 1869 2528 4362
Size: 1869 659 1834
Fragments arranged by size:
1869 1834 659
PpuMI rG'GwC_Cy
Cuts at: 0 210 1596 2645 3357 3888 4362
Size: 210 1386 1049 712 531 474
Fragments arranged by size:
1386 1049 712 531 474 210
PshAI GACnn'nnGTC
Cuts at: 0 2573 4362
Size: 2573 1789
PspOMI G'GGCC_C
Cuts at: 0 1552 3756 4129 4362
Size: 1552 2204 373 233
Fragments arranged by size:
2204 1552 373 233
PstI C_TGCA'G
Cuts at: 0 123 728 972 1238 4362
Size: 123 605 244 266 3124
Fragments arranged by size:
3124 605 266 244 123
PvuI CG_AT'CG
Cuts at: 0 3660 4362
Size: 3660 702
PvuII CAG'CTG
Cuts at: 0 1122 3280 4362
Size: 1122 2158 1082
Fragments arranged by size:
2158 1122 1082
SacII CC_GC'GG
Cuts at: 0 752 1196 4362
Size: 752 444 3166
Fragments arranged by size:
3166 752 444
SalI G'TCGA_C
Cuts at: 0 1258 4362
Size: 1258 3104
SanDI GG'GwC_CC
Cuts at: 0 1596 4362
Size: 1596 2766
SfcI C'TryA_G
Cuts at: 0 119 371 724 968 1234 4362
Size: 119 252 353 244 266 3128
Fragments arranged by size:
3128 353 266 252 244 119
SfoI GGC'GCC
Cuts at: 0 110 4362
Size: 110 4252
SgrAI Cr'CCGG_yG
Cuts at: 0 12 4362
Size: 12 4350
SmaI CCC'GGG
Cuts at: 0 3362 4362
Size: 3362 1000
SmlI C'TyrA_G
Cuts at: 0 389 1789 2245 4362
Size: 389 1400 456 2117
Fragments arranged by size:
2117 1400 456 389
SpeI A'CTAG_T
Cuts at: 0 3060 4362
Size: 3060 1302
SspI AAT'ATT
Cuts at: 0 170 336 588 1952 4362
Size: 170 166 252 1364 2410
Fragments arranged by size:
2410 1364 252 170 166
StyI C'CwwG_G
Cuts at: 0 3380 4362
Size: 3380 982
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 646 994 1411 3955 4362
Size: 646 348 417 2544 407
Fragments arranged by size:
2544 646 417 407 348
TatI w'GTAC_w
Cuts at: 0 1801 1828 4362
Size: 1801 27 2534
Fragments arranged by size:
2534 1801 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1359 2656 3723 4362
Size: 1359 1297 1067 639
Fragments arranged by size:
1359 1297 1067 639
XmaI C'CCGG_G
Cuts at: 0 3360 4362
Size: 3360 1002
XmnI GAAnn'nnTTC
Cuts at: 0 620 4362
Size: 620 3742
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EciI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII KasI KpnI MmeI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuI PvuII
SacII SalI SanDI SfcI SfoI SgrAI SmaI SmlI
SpeI SspI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AgeI AloI AscI AsiSI AvrII BamHI
BclI BfrBI BglI BglII BmgBI BmtI BplI BsaBI
BsaXI BsiWI BspEI BspHI BsrGI BssHII BstZ17I ClaI
CspCI EagI EarI EcoICRI EcoRI EcoRV FseI FspAI
HindIII HpaI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PsiI
PspXI PsrI RsrII SacI SapI SbfI ScaI SexAI
SfiI SgfI SnaBI SphI SrfI StuI SwaI TstI
Tth111I XbaI XhoI ZraI