Restriction map for
pF1KSDA1074
(Linear) (Six-base) MAPSORT of: KIAA1074.seq Check: 4628 from: 1 to: 5127
KIAA1074.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 4257 5041 5127
Size: 4257 784 86
Fragments arranged by size:
4257 784 86
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1736 2848 3925 3992 5127
Size: 743 993 1112 1077 67 1135
Fragments arranged by size:
1135 1112 1077 993 743 67
AflII C'TTAA_G
Cuts at: 0 732 5127
Size: 732 4395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2489 2521 3080 3112 5127
Size: 2489 32 559 32 2015
Fragments arranged by size:
2489 2015 559 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3835 4020 5127
Size: 3835 185 1107
Fragments arranged by size:
3835 1107 185
ApaLI G'TGCA_C
Cuts at: 0 1186 5127
Size: 1186 3941
ApoI r'AATT_y
Cuts at: 0 586 870 1281 1884 2115 2627 2685 2694
Size: 586 284 411 603 231 512 58 9
Cuts at: 2694 2961 3141 3963 4138 4280 5127
Size: 267 180 822 175 142 847
Fragments arranged by size:
847 822 603 586 512 411 284 267
231 180 175 142 58 9
AseI AT'TA_AT
Cuts at: 0 1741 3491 3746 4193 5127
Size: 1741 1750 255 447 934
Fragments arranged by size:
1750 1741 934 447 255
AvaI C'yCGr_G
Cuts at: 0 2594 5127
Size: 2594 2533
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3322 3355 5127
Size: 3322 33 1772
Fragments arranged by size:
3322 1772 33
BanI G'GyrC_C
Cuts at: 0 97 5127
Size: 97 5030
BanII G_rGCy'C
Cuts at: 0 43 5127
Size: 43 5084
BbeI G_GCGC'C
Cuts at: 0 101 5127
Size: 101 5026
BbsI GAAGACnn'nnnn_
Cuts at: 0 731 797 2273 2864 2875 3487 3547 4637
Size: 731 66 1476 591 11 612 60 1090
Cuts at: 4637 5127
Size: 490
Fragments arranged by size:
1476 1090 731 612 591 490 66 60
11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2342 2376 2575 2609 5127
Size: 2342 34 199 34 2518
Fragments arranged by size:
2518 2342 199 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 668 1419 4260 5127
Size: 668 751 2841 867
Fragments arranged by size:
2841 867 751 668
BclI T'GATC_A
Cuts at: 0 1770 3057 4370 5127
Size: 1770 1287 1313 757
Fragments arranged by size:
1770 1313 1287 757
BglII A'GATC_T
Cuts at: 0 135 4590 4780 4854 5067 5127
Size: 135 4455 190 74 213 60
Fragments arranged by size:
4455 213 190 135 74 60
BlpI GC'TnA_GC
Cuts at: 0 831 5127
Size: 831 4296
Bme1580I G_kGCm'C
Cuts at: 0 1190 5127
Size: 1190 3937
BmgBI CAC'GTC
Cuts at: 0 123 5127
Size: 123 5004
BmrI ACTGGGnnnn_n'
Cuts at: 0 2907 5127
Size: 2907 2220
BmtI G_CTAG'C
Cuts at: 0 4738 5127
Size: 4738 389
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1290 3271 3422 5127
Size: 1290 1981 151 1705
Fragments arranged by size:
1981 1705 1290 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2536 2992 3742 4255 5127
Size: 2536 456 750 513 872
Fragments arranged by size:
2536 872 750 513 456
Bpu10I CC'TnA_GC
Cuts at: 0 202 3298 5127
Size: 202 3096 1829
Fragments arranged by size:
3096 1829 202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4502 4610 4962 5127
Size: 4502 108 352 165
Fragments arranged by size:
4502 352 165 108
BsaI GGTCTCn'nnnn_
Cuts at: 0 122 5127
Size: 122 5005
BsaBI GATnn'nnATC
Cuts at: 0 416 3056 3493 5127
Size: 416 2640 437 1634
Fragments arranged by size:
2640 1634 437 416
BsaHI Gr'CG_yC
Cuts at: 0 98 5127
Size: 98 5029
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2489 2519 3388 3418 3715 3745 5127
Size: 2489 30 869 30 297 30 1382
Fragments arranged by size:
2489 1382 869 297 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 3409 3949 4306 5127
Size: 75 3334 540 357 821
Fragments arranged by size:
3334 821 540 357 75
BseYI C'CCAG_C
Cuts at: 0 1312 5127
Size: 1312 3815
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 5127
Size: 205 4922
BsiHKAI G_wGCw'C
Cuts at: 0 1140 1190 1849 2365 5127
Size: 1140 50 659 516 2762
Fragments arranged by size:
2762 1140 659 516 50
BsmI GAATG_Cn'
Cuts at: 0 2610 2955 5127
Size: 2610 345 2172
Fragments arranged by size:
2610 2172 345
Bsp1286I G_dGCh'C
Cuts at: 0 43 1140 1190 1849 2365 5127
Size: 43 1097 50 659 516 2762
Fragments arranged by size:
2762 1097 659 516 50 43
BspHI T'CATG_A
Cuts at: 0 2706 5127
Size: 2706 2421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4234 5127
Size: 4234 893
BsrDI GCAATG_nn'
Cuts at: 0 1097 2960 5127
Size: 1097 1863 2167
Fragments arranged by size:
2167 1863 1097
BsrGI T'GTAC_A
Cuts at: 0 360 1545 5097 5127
Size: 360 1185 3552 30
Fragments arranged by size:
3552 1185 360 30
BssSI C'ACGA_G
Cuts at: 0 3243 5127
Size: 3243 1884
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2920 5127
Size: 2920 2207
BstYI r'GATC_y
Cuts at: 0 135 146 191 4590 4780 4854 5067 5127
Size: 135 11 45 4399 190 74 213 60
Fragments arranged by size:
4399 213 190 135 74 60 45 11
BtgI C'CryG_G
Cuts at: 0 432 4149 4909 5127
Size: 432 3717 760 218
Fragments arranged by size:
3717 760 432 218
BtsI GCAGTG_nn'
Cuts at: 0 440 5127
Size: 440 4687
DraI TTT'AAA
Cuts at: 0 3198 3773 4160 5108 5127
Size: 3198 575 387 948 19
Fragments arranged by size:
3198 948 575 387 19
DraIII CAC_nnn'GTG
Cuts at: 0 4828 5127
Size: 4828 299
EarI CTCTTCn'nnn_
Cuts at: 0 368 452 1589 2387 2392 2450 5127
Size: 368 84 1137 798 5 58 2677
Fragments arranged by size:
2677 1137 798 368 84 58 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 72 5127
Size: 72 5055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1736 2536 2848 2992 3742 3925 3992
Size: 743 993 800 312 144 750 183 67
Cuts at: 3992 4255 5127
Size: 263 872
Fragments arranged by size:
993 872 800 750 743 312 263 183
144 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 735 5127
Size: 735 4392
EcoO109I rG'GnC_Cy
Cuts at: 0 2829 3294 5127
Size: 2829 465 1833
Fragments arranged by size:
2829 1833 465
EcoRI G'AATT_C
Cuts at: 0 1884 2627 2685 2694 5127
Size: 1884 743 58 9 2433
Fragments arranged by size:
2433 1884 743 58 9
EcoRV GAT'ATC
Cuts at: 0 1477 5127
Size: 1477 3650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 231 4110 5127
Size: 231 3879 1017
Fragments arranged by size:
3879 1017 231
HaeII r_GCGC'y
Cuts at: 0 101 5127
Size: 101 5026
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 703 735 837 869 1198 1230
Size: 460 32 211 32 102 32 329 32
Cuts at: 1230 1234 1266 3168 3200 3388 3420 3715 3747
Size: 4 32 1902 32 188 32 295 32
Cuts at: 3747 3846 3878 4203 4235 4285 4317 4582 4614
Size: 99 32 325 32 50 32 265 32
Cuts at: 4614 5127
Size: 513
Fragments arranged by size:
1902 513 460 329 325 295 265 211
188 102 99 50 32 32 32 32
32 32 32 32 32 32 32 32
4
HincII GTy'rAC
Cuts at: 0 4746 5127
Size: 4746 381
HindIII A'AGCT_T
Cuts at: 0 828 3874 5127
Size: 828 3046 1253
Fragments arranged by size:
3046 1253 828
KasI G'GCGC_C
Cuts at: 0 97 5127
Size: 97 5030
MfeI C'AATT_G
Cuts at: 0 3802 5127
Size: 3802 1325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 4337 4943 5127
Size: 611 3726 606 184
Fragments arranged by size:
3726 611 606 184
MslI CAynn'nnrTG
Cuts at: 0 1501 1697 2705 3053 4023 5127
Size: 1501 196 1008 348 970 1104
Fragments arranged by size:
1501 1104 1008 970 348 196
MspA1I CmG'CkG
Cuts at: 0 1564 2944 5127
Size: 1564 1380 2183
Fragments arranged by size:
2183 1564 1380
NarI GG'CG_CC
Cuts at: 0 98 5127
Size: 98 5029
NcoI C'CATG_G
Cuts at: 0 432 4149 5127
Size: 432 3717 978
Fragments arranged by size:
3717 978 432
NdeI CA'TA_TG
Cuts at: 0 885 5127
Size: 885 4242
NheI G'CTAG_C
Cuts at: 0 4734 5127
Size: 4734 393
NspI r_CATG'y
Cuts at: 0 1446 2745 5127
Size: 1446 1299 2382
Fragments arranged by size:
2382 1446 1299
PflMI CCAn_nnn'nTGG
Cuts at: 0 1897 2514 5127
Size: 1897 617 2613
Fragments arranged by size:
2613 1897 617
PfoI T'CCnGG_A
Cuts at: 0 862 2180 5127
Size: 862 1318 2947
Fragments arranged by size:
2947 1318 862
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2489 2521 5127
Size: 2489 32 2606
Fragments arranged by size:
2606 2489 32
PpuMI rG'GwC_Cy
Cuts at: 0 2829 3294 5127
Size: 2829 465 1833
Fragments arranged by size:
2829 1833 465
PstI C_TGCA'G
Cuts at: 0 1323 4245 5127
Size: 1323 2922 882
Fragments arranged by size:
2922 1323 882
PvuII CAG'CTG
Cuts at: 0 1564 2944 5127
Size: 1564 1380 2183
Fragments arranged by size:
2183 1564 1380
SapI GCTCTTCn'nnn_
Cuts at: 0 452 1589 2450 5127
Size: 452 1137 861 2677
Fragments arranged by size:
2677 1137 861 452
SfcI C'TryA_G
Cuts at: 0 1319 3389 4241 4336 5127
Size: 1319 2070 852 95 791
Fragments arranged by size:
2070 1319 852 791 95
SfoI GGC'GCC
Cuts at: 0 99 5127
Size: 99 5028
SmlI C'TyrA_G
Cuts at: 0 732 2594 4517 4625 4941 5127
Size: 732 1862 1923 108 316 186
Fragments arranged by size:
1923 1862 732 316 186 108
SpeI A'CTAG_T
Cuts at: 0 4165 5127
Size: 4165 962
SphI G_CATG'C
Cuts at: 0 1446 5127
Size: 1446 3681
SspI AAT'ATT
Cuts at: 0 513 5127
Size: 513 4614
StuI AGG'CCT
Cuts at: 0 1983 5127
Size: 1983 3144
StyI C'CwwG_G
Cuts at: 0 35 432 1255 4149 5127
Size: 35 397 823 2894 978
Fragments arranged by size:
2894 978 823 397 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 146 5127
Size: 146 4981
TatI w'GTAC_w
Cuts at: 0 360 1545 2280 4314 5097 5127
Size: 360 1185 735 2034 783 30
Fragments arranged by size:
2034 1185 783 735 360 30
XbaI T'CTAG_A
Cuts at: 0 3028 4674 5127
Size: 3028 1646 453
Fragments arranged by size:
3028 1646 453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4758 4812 5127
Size: 4758 54 315
Fragments arranged by size:
4758 315 54
XhoI C'TCGA_G
Cuts at: 0 2594 5127
Size: 2594 2533
XmnI GAAnn'nnTTC
Cuts at: 0 965 2861 5127
Size: 965 1896 2266
Fragments arranged by size:
2266 1896 965
Enzymes that do cut:
AccI AcuI AflII AloI AlwNI ApaLI ApoI AseI
AvaI BaeI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BssSI BstXI BstYI BtgI BtsI DraI DraIII
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KasI MfeI MmeI MslI
MspA1I NarI NcoI NdeI NheI NspI PflMI PfoI
PpiI PpuMI PstI PvuII SapI SfcI SfoI SmlI
SpeI SphI SspI StuI StyI TaqII TatI XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI AscI AsiSI AvrII BamHI BbvCI
BfrBI BglI BsaAI BsaWI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EaeI EagI EcoICRI FseI
FspI FspAI HpaI KpnI MluI MscI NaeI NgoMIV
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SrfI SwaI TstI Tth111I XmaI
ZraI