Restriction map for
pF1KSDA1064
(Linear) (Six-base) MAPSORT of: KIAA1064.seq Check: 9194 from: 1 to: 3909
KIAA1064.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1453 3909
Size: 1453 2456
AatII G_ACGT'C
Cuts at: 0 2485 3909
Size: 2485 1424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1516 2482 2486 3880 3909
Size: 328 1188 966 4 1394 29
Fragments arranged by size:
1394 1188 966 328 29 4
AflIII A'CryG_T
Cuts at: 0 1721 3909
Size: 1721 2188
AhdI GACnn_n'nnGTC
Cuts at: 0 2856 3909
Size: 2856 1053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1624 3059 3909
Size: 1624 1435 850
Fragments arranged by size:
1624 1435 850
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3550 3582 3909
Size: 3550 32 327
Fragments arranged by size:
3550 327 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1707 3909
Size: 1707 2202
ApaI G_GGCC'C
Cuts at: 0 1661 1840 1847 1966 1996 2243 2304 2734
Size: 1661 179 7 119 30 247 61 430
Cuts at: 2734 2793 3059 3909
Size: 59 266 850
Fragments arranged by size:
1661 850 430 266 247 179 119 61
59 30 7
ApaLI G'TGCA_C
Cuts at: 0 3739 3909
Size: 3739 170
AvaI C'yCGr_G
Cuts at: 0 13 995 1654 2833 2941 3078 3212 3275
Size: 13 982 659 1179 108 137 134 63
Cuts at: 3275 3324 3450 3712 3733 3909
Size: 49 126 262 21 176
Fragments arranged by size:
1179 982 659 262 176 137 134 126
108 63 49 21 13
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2406 2439 3909
Size: 2406 33 1470
Fragments arranged by size:
2406 1470 33
BamHI G'GATC_C
Cuts at: 0 2315 3909
Size: 2315 1594
BanI G'GyrC_C
Cuts at: 0 1465 2263 2837 3082 3109 3303 3502 3625
Size: 1465 798 574 245 27 194 199 123
Cuts at: 3625 3718 3909
Size: 93 191
Fragments arranged by size:
1465 798 574 245 199 194 191 123
93 27
BanII G_rGCy'C
Cuts at: 0 994 1430 1661 1840 1847 1966 1996 2126
Size: 994 436 231 179 7 119 30 130
Cuts at: 2126 2219 2243 2304 2734 2793 3059 3541 3563
Size: 93 24 61 430 59 266 482 22
Cuts at: 3563 3799 3909
Size: 236 110
Fragments arranged by size:
994 482 436 430 266 236 231 179
130 119 110 93 61 59 30 24
22 7
BbeI G_GCGC'C
Cuts at: 0 2267 3086 3113 3307 3909
Size: 2267 819 27 194 602
Fragments arranged by size:
2267 819 602 194 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 1088 2470 3909
Size: 1088 1382 1439
Fragments arranged by size:
1439 1382 1088
BbvCI CC'TCA_GC
Cuts at: 0 2289 2590 3299 3521 3909
Size: 2289 301 709 222 388
Fragments arranged by size:
2289 709 388 301 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1176 1210 1253 1287 3909
Size: 1176 34 43 34 2622
Fragments arranged by size:
2622 1176 43 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 213 3909
Size: 213 3696
BfrBI ATG'CAT
Cuts at: 0 1884 3909
Size: 1884 2025
BglI GCCn_nnn'nGGC
Cuts at: 0 112 1037 2000 2299 2729 3550 3909
Size: 112 925 963 299 430 821 359
Fragments arranged by size:
963 925 821 430 359 299 112
BlpI GC'TnA_GC
Cuts at: 0 2515 3427 3909
Size: 2515 912 482
Fragments arranged by size:
2515 912 482
Bme1580I G_kGCm'C
Cuts at: 0 1661 1840 1847 1966 1996 2159 2243 2304
Size: 1661 179 7 119 30 163 84 61
Cuts at: 2304 2734 2793 2840 2987 2996 3059 3507 3628
Size: 430 59 47 147 9 63 448 121
Cuts at: 3628 3723 3743 3758 3909
Size: 95 20 15 151
Fragments arranged by size:
1661 448 430 179 163 151 147 121
119 95 84 63 61 59 47 30
20 15 9 7
BmgBI CAC'GTC
Cuts at: 0 2895 3909
Size: 2895 1014
BmrI ACTGGGnnnn_n'
Cuts at: 0 434 1370 3197 3400 3909
Size: 434 936 1827 203 509
Fragments arranged by size:
1827 936 509 434 203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3324 3909
Size: 3324 585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2048 2233 2530 3909
Size: 2048 185 297 1379
Fragments arranged by size:
2048 1379 297 185
Bpu10I CC'TnA_GC
Cuts at: 0 2201 2289 2590 2902 3299 3521 3554 3798
Size: 2201 88 301 312 397 222 33 244
Cuts at: 3798 3909
Size: 111
Fragments arranged by size:
2201 397 312 301 244 222 111 88
33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2403 2490 3133 3909
Size: 2403 87 643 776
Fragments arranged by size:
2403 776 643 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 205 818 1214 3909
Size: 205 613 396 2695
Fragments arranged by size:
2695 613 396 205
BsaHI Gr'CG_yC
Cuts at: 0 104 2264 2482 3083 3110 3304 3909
Size: 104 2160 218 601 27 194 605
Fragments arranged by size:
2160 605 601 218 194 104 27
BsaWI w'CCGG_w
Cuts at: 0 226 1127 2404 3909
Size: 226 901 1277 1505
Fragments arranged by size:
1505 1277 901 226
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 901 928 931 958 3550 3580 3909
Size: 901 27 3 27 2592 30 329
Fragments arranged by size:
2592 901 329 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 118 223 301 375 637 640 836
Size: 77 41 105 78 74 262 3 196
Cuts at: 836 898 1061 1550 2350 2353 2356 2367 2785
Size: 62 163 489 800 3 3 11 418
Cuts at: 2785 2788 3162 3261 3909
Size: 3 374 99 648
Fragments arranged by size:
800 648 489 418 374 262 196 163
105 99 78 77 74 62 41 11
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1791 2529 2734 2911 3506 3728 3814 3909
Size: 1791 738 205 177 595 222 86 95
Fragments arranged by size:
1791 738 595 222 205 177 95 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1194 3019 3909
Size: 1194 1825 890
Fragments arranged by size:
1825 1194 890
BsiEI CG_ry'CG
Cuts at: 0 711 2254 3179 3383 3909
Size: 711 1543 925 204 526
Fragments arranged by size:
1543 925 711 526 204
BsiHKAI G_wGCw'C
Cuts at: 0 1430 2192 2207 2933 3541 3743 3909
Size: 1430 762 15 726 608 202 166
Fragments arranged by size:
1430 762 726 608 202 166 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 890 1411 3909
Size: 890 521 2498
Fragments arranged by size:
2498 890 521
Bsp1286I G_dGCh'C
Cuts at: 0 994 1430 1661 1840 1847 1966 1996 2126
Size: 994 436 231 179 7 119 30 130
Cuts at: 2126 2159 2192 2207 2219 2243 2304 2734 2793
Size: 33 33 15 12 24 61 430 59
Cuts at: 2793 2840 2933 2987 2996 3059 3507 3541 3563
Size: 47 93 54 9 63 448 34 22
Cuts at: 3563 3628 3723 3743 3758 3799 3909
Size: 65 95 20 15 41 110
Fragments arranged by size:
994 448 436 430 231 179 130 119
110 95 93 65 63 61 59 54
47 41 34 33 33 30 24 22
20 15 15 12 9 7
BspEI T'CCGG_A
Cuts at: 0 226 3909
Size: 226 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1453 3755 3909
Size: 1453 2302 154
Fragments arranged by size:
2302 1453 154
BsrBI CCG'CTC
Cuts at: 0 147 330 944 2787 3355 3431 3909
Size: 147 183 614 1843 568 76 478
Fragments arranged by size:
1843 614 568 478 183 147 76
BsrDI GCAATG_nn'
Cuts at: 0 3534 3909
Size: 3534 375
BsrFI r'CCGG_y
Cuts at: 0 680 1677 2249 2266 2570 3166 3382 3486
Size: 680 997 572 17 304 596 216 104
Cuts at: 3486 3610 3656 3909
Size: 124 46 253
Fragments arranged by size:
997 680 596 572 304 253 216 124
104 46 17
BsrGI T'GTAC_A
Cuts at: 0 1724 3909
Size: 1724 2185
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1707 2844 3012 3747 3909
Size: 1707 1137 168 735 162
Fragments arranged by size:
1707 1137 735 168 162
BstEII G'GTnAC_C
Cuts at: 0 451 3909
Size: 451 3458
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1360 2727 3909
Size: 1360 1367 1182
Fragments arranged by size:
1367 1360 1182
BstYI r'GATC_y
Cuts at: 0 1736 2315 3909
Size: 1736 579 1594
Fragments arranged by size:
1736 1594 579
Bsu36I CC'TnA_GG
Cuts at: 0 2311 3909
Size: 2311 1598
BtgI C'CryG_G
Cuts at: 0 705 756 774 981 1769 1988 2854 2996
Size: 705 51 18 207 788 219 866 142
Cuts at: 2996 3047 3089 3632 3887 3909
Size: 51 42 543 255 22
Fragments arranged by size:
866 788 705 543 255 219 207 142
51 51 42 22 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 41 413 497 3909
Size: 41 372 84 3412
Fragments arranged by size:
3412 372 84 41
BtsI GCAGTG_nn'
Cuts at: 0 2451 2529 2987 3426 3909
Size: 2451 78 458 439 483
Fragments arranged by size:
2451 483 458 439 78
DraI TTT'AAA
Cuts at: 0 3873 3909
Size: 3873 36
EaeI y'GGCC_r
Cuts at: 0 113 708 1445 2251 3176 3380 3909
Size: 113 595 737 806 925 204 529
Fragments arranged by size:
925 806 737 595 529 204 113
EagI C'GGCC_G
Cuts at: 0 708 2251 3176 3380 3909
Size: 708 1543 925 204 529
Fragments arranged by size:
1543 925 708 529 204
EarI CTCTTCn'nnn_
Cuts at: 0 352 836 854 1409 2159 2200 3769 3909
Size: 352 484 18 555 750 41 1569 140
Fragments arranged by size:
1569 750 555 484 352 140 41 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1079 3416 3909
Size: 1079 2337 493
Fragments arranged by size:
2337 1079 493
EcoICRI GAG'CTC
Cuts at: 0 1428 3539 3909
Size: 1428 2111 370
Fragments arranged by size:
2111 1428 370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1516 2048 2233 2482 2486 2530 3880
Size: 328 1188 532 185 249 4 44 1350
Cuts at: 3880 3909
Size: 29
Fragments arranged by size:
1350 1188 532 328 249 185 44 29
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2548 3909
Size: 2548 1361
EcoO109I rG'GnC_Cy
Cuts at: 0 17 232 1658 1837 1855 1963 1993 2239
Size: 17 215 1426 179 18 108 30 246
Cuts at: 2239 2240 2300 2301 2535 2598 2656 2731 2790
Size: 1 60 1 234 63 58 75 59
Cuts at: 2790 2850 2946 3055 3056 3225 3909
Size: 60 96 109 1 169 684
Fragments arranged by size:
1426 684 246 234 215 179 169 109
108 96 75 63 60 60 59 58
30 18 17 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2728 2931 3909
Size: 2728 203 978
Fragments arranged by size:
2728 978 203
FseI GG_CCGG'CC
Cuts at: 0 2253 3909
Size: 2253 1656
HaeII r_GCGC'y
Cuts at: 0 1209 2267 3078 3086 3113 3307 3909
Size: 1209 1058 811 8 27 194 602
Fragments arranged by size:
1209 1058 811 602 194 27 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 391 423 901 933 1678 1710
Size: 277 32 82 32 478 32 745 32
Cuts at: 1710 2212 2244 2842 2874 3838 3870 3909
Size: 502 32 598 32 964 32 39
Fragments arranged by size:
964 745 598 502 478 277 82 39
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 2263 3082 3109 3303 3909
Size: 2263 819 27 194 606
Fragments arranged by size:
2263 819 606 194 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 1142 1609 3909
Size: 155 987 467 2300
Fragments arranged by size:
2300 987 467 155
MslI CAynn'nnrTG
Cuts at: 0 272 1678 1957 2116 3909
Size: 272 1406 279 159 1793
Fragments arranged by size:
1793 1406 279 272 159
MspA1I CmG'CkG
Cuts at: 0 707 776 1707 1779 2865 2998 3688 3909
Size: 707 69 931 72 1086 133 690 221
Fragments arranged by size:
1086 931 707 690 221 133 72 69
NaeI GCC'GGC
Cuts at: 0 2251 2572 3488 3909
Size: 2251 321 916 421
Fragments arranged by size:
2251 916 421 321
NarI GG'CG_CC
Cuts at: 0 2264 3083 3110 3304 3909
Size: 2264 819 27 194 605
Fragments arranged by size:
2264 819 605 194 27
NcoI C'CATG_G
Cuts at: 0 1988 3909
Size: 1988 1921
NdeI CA'TA_TG
Cuts at: 0 483 3909
Size: 483 3426
NgoMIV G'CCGG_C
Cuts at: 0 2249 2570 3486 3909
Size: 2249 321 916 423
Fragments arranged by size:
2249 916 423 321
NruI TCG'CGA
Cuts at: 0 768 3909
Size: 768 3141
NsiI A_TGCA'T
Cuts at: 0 1886 3909
Size: 1886 2023
NspI r_CATG'y
Cuts at: 0 520 576 1392 1725 1896 1908 1920 1932
Size: 520 56 816 333 171 12 12 12
Cuts at: 1932 1944 1956 3909
Size: 12 12 1953
Fragments arranged by size:
1953 816 520 333 171 56 12 12
12 12 12
PasI CC'CwG_GG
Cuts at: 0 1825 1840 1861 3909
Size: 1825 15 21 2048
Fragments arranged by size:
2048 1825 21 15
PciI A'CATG_T
Cuts at: 0 1721 3909
Size: 1721 2188
PflMI CCAn_nnn'nTGG
Cuts at: 0 2044 3814 3909
Size: 2044 1770 95
Fragments arranged by size:
2044 1770 95
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3550 3582 3909
Size: 3550 32 327
Fragments arranged by size:
3550 327 32
PpuMI rG'GwC_Cy
Cuts at: 0 17 1855 2535 2598 2656 2850 2946 3909
Size: 17 1838 680 63 58 194 96 963
Fragments arranged by size:
1838 963 680 194 96 63 58 17
PspOMI G'GGCC_C
Cuts at: 0 1657 1836 1843 1962 1992 2239 2300 2730
Size: 1657 179 7 119 30 247 61 430
Cuts at: 2730 2789 3055 3909
Size: 59 266 854
Fragments arranged by size:
1657 854 430 266 247 179 119 61
59 30 7
PstI C_TGCA'G
Cuts at: 0 1712 2870 3248 3681 3909
Size: 1712 1158 378 433 228
Fragments arranged by size:
1712 1158 433 378 228
PvuII CAG'CTG
Cuts at: 0 1707 1779 2865 3909
Size: 1707 72 1086 1044
Fragments arranged by size:
1707 1086 1044 72
SacI G_AGCT'C
Cuts at: 0 1430 3541 3909
Size: 1430 2111 368
Fragments arranged by size:
2111 1430 368
SacII CC_GC'GG
Cuts at: 0 708 777 2999 3909
Size: 708 69 2222 910
Fragments arranged by size:
2222 910 708 69
SanDI GG'GwC_CC
Cuts at: 0 17 1855 2535 2598 2656 2850 3909
Size: 17 1838 680 63 58 194 1059
Fragments arranged by size:
1838 1059 680 194 63 58 17
SapI GCTCTTCn'nnn_
Cuts at: 0 2159 3909
Size: 2159 1750
ScaI AGT'ACT
Cuts at: 0 916 1192 3909
Size: 916 276 2717
Fragments arranged by size:
2717 916 276
SfcI C'TryA_G
Cuts at: 0 738 1708 2866 3244 3677 3909
Size: 738 970 1158 378 433 232
Fragments arranged by size:
1158 970 738 433 378 232
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2000 3909
Size: 2000 1909
SfoI GGC'GCC
Cuts at: 0 2265 3084 3111 3305 3909
Size: 2265 819 27 194 604
Fragments arranged by size:
2265 819 604 194 27
SgrAI Cr'CCGG_yG
Cuts at: 0 2266 3909
Size: 2266 1643
SmaI CCC'GGG
Cuts at: 0 15 1656 2835 3080 3214 3277 3735 3909
Size: 15 1641 1179 245 134 63 458 174
Fragments arranged by size:
1641 1179 458 245 174 134 63 15
SmlI C'TyrA_G
Cuts at: 0 2418 2469 3148 3909
Size: 2418 51 679 761
Fragments arranged by size:
2418 761 679 51
SphI G_CATG'C
Cuts at: 0 520 3909
Size: 520 3389
SrfI GCCC'GGGC
Cuts at: 0 1656 3080 3909
Size: 1656 1424 829
Fragments arranged by size:
1656 1424 829
StyI C'CwwG_G
Cuts at: 0 689 920 947 1988 2744 3512 3548 3668
Size: 689 231 27 1041 756 768 36 120
Cuts at: 3668 3909
Size: 241
Fragments arranged by size:
1041 768 756 689 241 231 120 36
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1426 1783 2001 3801 3909
Size: 1426 357 218 1800 108
Fragments arranged by size:
1800 1426 357 218 108
TatI w'GTAC_w
Cuts at: 0 461 470 914 1190 1724 3909
Size: 461 9 444 276 534 2185
Fragments arranged by size:
2185 534 461 444 276 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3597 3629 3909
Size: 3597 32 280
Fragments arranged by size:
3597 280 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1776 3018 3909
Size: 1776 1242 891
Fragments arranged by size:
1776 1242 891
XmaI C'CCGG_G
Cuts at: 0 13 1654 2833 3078 3212 3275 3733 3909
Size: 13 1641 1179 245 134 63 458 176
Fragments arranged by size:
1641 1179 458 245 176 134 63 13
ZraI GAC'GTC
Cuts at: 0 2483 3909
Size: 2483 1426
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI HaeII Hin4I KasI MmeI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspI
PasI PciI PflMI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SapI ScaI SfcI SfiI SfoI
SgrAI SmaI SmlI SphI SrfI StyI TaqII TatI
TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AleI ApoI
AscI AseI AsiSI AvrII BclI BglII BmtI BsaAI
BsaBI BsiWI BsmI BspHI BssHII BssSI BstBI BstZ17I
ClaI CspCI DraIII DrdI EcoRI EcoRV FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NheI
NotI PacI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SbfI SexAI SgfI SnaBI
SpeI SspI StuI SwaI Tth111I XbaI XhoI XmnI