Restriction map for
pF1KSDA1056
(Linear) (Six-base) MAPSORT of: KIAA1056.seq Check: 6260 from: 1 to: 2586
KIAA1056.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2025 2078 2381 2468 2586
Size: 2025 53 303 87 118
Fragments arranged by size:
2025 303 118 87 53
AatII G_ACGT'C
Cuts at: 0 1535 2586
Size: 1535 1051
Acc65I G'GTAC_C
Cuts at: 0 31 2586
Size: 31 2555
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2163 2167 2586
Size: 2163 4 419
Fragments arranged by size:
2163 419 4
AflIII A'CryG_T
Cuts at: 0 1319 2586
Size: 1319 1267
AhdI GACnn_n'nnGTC
Cuts at: 0 2139 2484 2586
Size: 2139 345 102
Fragments arranged by size:
2139 345 102
AleI CACnn'nnGTG
Cuts at: 0 1359 2586
Size: 1359 1227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1594 2531 2586
Size: 1594 937 55
Fragments arranged by size:
1594 937 55
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 2586
Size: 139 32 2415
Fragments arranged by size:
2415 139 32
AlwNI CAG_nnn'CTG
Cuts at: 0 180 810 1285 1401 1445 1454 1809 2088
Size: 180 630 475 116 44 9 355 279
Cuts at: 2088 2569 2586
Size: 481 17
Fragments arranged by size:
630 481 475 355 279 180 116 44
17 9
ApaI G_GGCC'C
Cuts at: 0 1627 1910 2586
Size: 1627 283 676
Fragments arranged by size:
1627 676 283
ApaLI G'TGCA_C
Cuts at: 0 1408 2586
Size: 1408 1178
ApoI r'AATT_y
Cuts at: 0 557 2586
Size: 557 2029
AvaI C'yCGr_G
Cuts at: 0 1947 2586
Size: 1947 639
AvrII C'CTAG_G
Cuts at: 0 1917 2586
Size: 1917 669
BanI G'GyrC_C
Cuts at: 0 31 1468 2296 2398 2586
Size: 31 1437 828 102 188
Fragments arranged by size:
1437 828 188 102 31
BanII G_rGCy'C
Cuts at: 0 227 314 458 1154 1222 1627 1910 2433
Size: 227 87 144 696 68 405 283 523
Cuts at: 2433 2586
Size: 153
Fragments arranged by size:
696 523 405 283 227 153 144 87
68
BbeI G_GCGC'C
Cuts at: 0 1472 2586
Size: 1472 1114
BbsI GAAGACnn'nnnn_
Cuts at: 0 696 1235 2586
Size: 696 539 1351
Fragments arranged by size:
1351 696 539
BbvCI CC'TCA_GC
Cuts at: 0 576 1027 1303 1567 1954 2586
Size: 576 451 276 264 387 632
Fragments arranged by size:
632 576 451 387 276 264
Bme1580I G_kGCm'C
Cuts at: 0 53 1201 1412 1627 1910 2401 2586
Size: 53 1148 211 215 283 491 185
Fragments arranged by size:
1148 491 283 215 211 185 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 452 1207 1229 2586
Size: 452 755 22 1357
Fragments arranged by size:
1357 755 452 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 202 374 642 817 895 2215 2586
Size: 72 130 172 268 175 78 1320 371
Fragments arranged by size:
1320 371 268 175 172 130 78 72
Bpu10I CC'TnA_GC
Cuts at: 0 328 576 1027 1303 1567 1954 2586
Size: 328 248 451 276 264 387 632
Fragments arranged by size:
632 451 387 328 276 264 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 2290 2314 2586
Size: 1342 948 24 272
Fragments arranged by size:
1342 948 272 24
BsaAI yAC'GTr
Cuts at: 0 696 2007 2586
Size: 696 1311 579
Fragments arranged by size:
1311 696 579
BsaHI Gr'CG_yC
Cuts at: 0 1469 1532 2586
Size: 1469 63 1054
Fragments arranged by size:
1469 1054 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 2586
Size: 67 30 2489
Fragments arranged by size:
2489 67 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 123 310 1841 2586
Size: 75 48 187 1531 745
Fragments arranged by size:
1531 745 187 75 48
BseYI C'CCAG_C
Cuts at: 0 822 1063 1247 1278 1462 1815 1979 2300
Size: 822 241 184 31 184 353 164 321
Cuts at: 2300 2409 2586
Size: 109 177
Fragments arranged by size:
822 353 321 241 184 184 177 164
109 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1740 1783 2546 2586
Size: 1740 43 763 40
Fragments arranged by size:
1740 763 43 40
BsiHKAI G_wGCw'C
Cuts at: 0 1412 2016 2586
Size: 1412 604 570
Fragments arranged by size:
1412 604 570
Bsp1286I G_dGCh'C
Cuts at: 0 53 227 314 458 1154 1201 1222 1412
Size: 53 174 87 144 696 47 21 190
Cuts at: 1412 1627 1910 2016 2401 2433 2586
Size: 215 283 106 385 32 153
Fragments arranged by size:
696 385 283 215 190 174 153 144
106 87 53 47 32 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1757 2025 2078 2381 2468 2586
Size: 1757 268 53 303 87 118
Fragments arranged by size:
1757 303 268 118 87 53
BssSI C'ACGA_G
Cuts at: 0 1487 2586
Size: 1487 1099
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1346 1454 1718 2285 2586
Size: 1346 108 264 567 301
Fragments arranged by size:
1346 567 301 264 108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1903 2586
Size: 1903 683
BstYI r'GATC_y
Cuts at: 0 117 654 2586
Size: 117 537 1932
Fragments arranged by size:
1932 537 117
Bsu36I CC'TnA_GG
Cuts at: 0 977 1127 2586
Size: 977 150 1459
Fragments arranged by size:
1459 977 150
BtgI C'CryG_G
Cuts at: 0 320 671 1670 2393 2586
Size: 320 351 999 723 193
Fragments arranged by size:
999 723 351 320 193
BtsI GCAGTG_nn'
Cuts at: 0 405 497 1443 1464 1714 2211 2586
Size: 405 92 946 21 250 497 375
Fragments arranged by size:
946 497 405 375 250 92 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1814 1849 2586
Size: 1814 35 737
Fragments arranged by size:
1814 737 35
EaeI y'GGCC_r
Cuts at: 0 2 374 1598 2586
Size: 2 372 1224 988
Fragments arranged by size:
1224 988 372 2
EarI CTCTTCn'nnn_
Cuts at: 0 446 552 906 1133 1897 2586
Size: 446 106 354 227 764 689
Fragments arranged by size:
764 689 446 354 227 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2028 2586
Size: 2028 558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 202 374 642 817 895 2163 2167
Size: 72 130 172 268 175 78 1268 4
Cuts at: 2167 2215 2586
Size: 48 371
Fragments arranged by size:
1268 371 268 175 172 130 78 72
48 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2377 2586
Size: 2377 209
EcoO109I rG'GnC_Cy
Cuts at: 0 155 235 807 858 993 1059 1299 1624
Size: 155 80 572 51 135 66 240 325
Cuts at: 1624 1846 1907 1914 2586
Size: 222 61 7 672
Fragments arranged by size:
672 572 325 240 222 155 135 80
66 61 51 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2331 2586
Size: 2331 255
FspI TGC'GCA
Cuts at: 0 1753 2586
Size: 1753 833
HaeII r_GCGC'y
Cuts at: 0 1472 2586
Size: 1472 1114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 1425 1457 2125 2157 2470 2502
Size: 67 32 1326 32 668 32 313 32
Cuts at: 2502 2586
Size: 84
Fragments arranged by size:
1326 668 313 84 67 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 867 2281 2586
Size: 867 1414 305
Fragments arranged by size:
1414 867 305
KasI G'GCGC_C
Cuts at: 0 1468 2586
Size: 1468 1118
KpnI G_GTAC'C
Cuts at: 0 35 2586
Size: 35 2551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 1669 2343 2513 2586
Size: 537 1132 674 170 73
Fragments arranged by size:
1132 674 537 170 73
MscI TGG'CCA
Cuts at: 0 4 376 1600 2586
Size: 4 372 1224 986
Fragments arranged by size:
1224 986 372 4
MslI CAynn'nnrTG
Cuts at: 0 1359 1576 1759 2586
Size: 1359 217 183 827
Fragments arranged by size:
1359 827 217 183
MspA1I CmG'CkG
Cuts at: 0 352 633 683 826 1526 1672 1815 2159
Size: 352 281 50 143 700 146 143 344
Cuts at: 2159 2276 2361 2511 2586
Size: 117 85 150 75
Fragments arranged by size:
700 352 344 281 150 146 143 143
117 85 75 50
NarI GG'CG_CC
Cuts at: 0 1469 2586
Size: 1469 1117
NcoI C'CATG_G
Cuts at: 0 320 671 2393 2586
Size: 320 351 1722 193
Fragments arranged by size:
1722 351 320 193
NdeI CA'TA_TG
Cuts at: 0 1577 1760 2586
Size: 1577 183 826
Fragments arranged by size:
1577 826 183
NspI r_CATG'y
Cuts at: 0 1323 1416 1782 2347 2518 2586
Size: 1323 93 366 565 171 68
Fragments arranged by size:
1323 565 366 171 93 68
PasI CC'CwG_GG
Cuts at: 0 46 268 428 2418 2586
Size: 46 222 160 1990 168
Fragments arranged by size:
1990 222 168 160 46
PciI A'CATG_T
Cuts at: 0 1319 2586
Size: 1319 1267
PfoI T'CCnGG_A
Cuts at: 0 179 833 1004 2234 2586
Size: 179 654 171 1230 352
Fragments arranged by size:
1230 654 352 179 171
PmlI CAC'GTG
Cuts at: 0 696 2586
Size: 696 1890
PpuMI rG'GwC_Cy
Cuts at: 0 155 1059 2586
Size: 155 904 1527
Fragments arranged by size:
1527 904 155
PshAI GACnn'nnGTC
Cuts at: 0 245 2586
Size: 245 2341
PspOMI G'GGCC_C
Cuts at: 0 1623 1906 2586
Size: 1623 283 680
Fragments arranged by size:
1623 680 283
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 395 427 2586
Size: 395 32 2159
Fragments arranged by size:
2159 395 32
PstI C_TGCA'G
Cuts at: 0 1290 1450 1459 2218 2586
Size: 1290 160 9 759 368
Fragments arranged by size:
1290 759 368 160 9
PvuII CAG'CTG
Cuts at: 0 352 633 826 2361 2511 2586
Size: 352 281 193 1535 150 75
Fragments arranged by size:
1535 352 281 193 150 75
SacII CC_GC'GG
Cuts at: 0 1673 2586
Size: 1673 913
SanDI GG'GwC_CC
Cuts at: 0 1059 2586
Size: 1059 1527
SapI GCTCTTCn'nnn_
Cuts at: 0 446 552 2586
Size: 446 106 2034
Fragments arranged by size:
2034 446 106
ScaI AGT'ACT
Cuts at: 0 1900 2586
Size: 1900 686
SfcI C'TryA_G
Cuts at: 0 1286 1446 1455 1606 2214 2586
Size: 1286 160 9 151 608 372
Fragments arranged by size:
1286 608 372 160 151 9
SfoI GGC'GCC
Cuts at: 0 1470 2586
Size: 1470 1116
SmaI CCC'GGG
Cuts at: 0 1949 2586
Size: 1949 637
SmlI C'TyrA_G
Cuts at: 0 1357 2305 2329 2586
Size: 1357 948 24 257
Fragments arranged by size:
1357 948 257 24
SphI G_CATG'C
Cuts at: 0 2518 2586
Size: 2518 68
StuI AGG'CCT
Cuts at: 0 364 751 2176 2586
Size: 364 387 1425 410
Fragments arranged by size:
1425 410 387 364
StyI C'CwwG_G
Cuts at: 0 320 534 671 1187 1917 1937 2393 2586
Size: 320 214 137 516 730 20 456 193
Fragments arranged by size:
730 516 456 320 214 193 137 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1059 1194 2284 2314 2586
Size: 1059 135 1090 30 272
Fragments arranged by size:
1090 1059 272 135 30
TatI w'GTAC_w
Cuts at: 0 828 1898 2586
Size: 828 1070 688
Fragments arranged by size:
1070 828 688
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 102 134 2586
Size: 102 32 2452
Fragments arranged by size:
2452 102 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 964 1624 2586
Size: 964 660 962
Fragments arranged by size:
964 962 660
XmaI C'CCGG_G
Cuts at: 0 1947 2586
Size: 1947 639
XmnI GAAnn'nnTTC
Cuts at: 0 2152 2586
Size: 2152 434
ZraI GAC'GTC
Cuts at: 0 1533 2586
Size: 1533 1053
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI
BanII BbeI BbsI BbvCI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HindIII KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NdeI NspI PasI
PciI PfoI PmlI PpuMI PshAI PspOMI PsrI PstI
PvuII SacII SanDI SapI ScaI SfcI SfoI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AscI AseI AsiSI
BaeI BamHI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BsaI BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI FspAI
HincII HpaI MfeI MluI NaeI NgoMIV NheI NotI
NruI NsiI PacI PflMI PmeI PpiI PsiI PspXI
PvuI RsrII SacI SalI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI