(Linear) (Six-base) MAPSORT of: KIAA1044.seq Check: 6385 from: 1 to: 1890 KIAA1044.orf With 182 enzymes: SgfI * October 24, 2006 15:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1448 1591 1890 Size: 1448 143 299 Fragments arranged by size: 1448 299 143 AatII G_ACGT'C Cuts at: 0 854 1890 Size: 854 1036 AccI GT'mk_AC Cuts at: 0 1087 1656 1890 Size: 1087 569 234 Fragments arranged by size: 1087 569 234 AclI AA'CG_TT Cuts at: 0 180 1890 Size: 180 1710 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 876 970 1209 1890 Size: 876 94 239 681 Fragments arranged by size: 876 681 239 94 AlwNI CAG_nnn'CTG Cuts at: 0 1583 1890 Size: 1583 307 ApoI r'AATT_y Cuts at: 0 288 1890 Size: 288 1602 AvaI C'yCGr_G Cuts at: 0 69 90 1890 Size: 69 21 1800 Fragments arranged by size: 1800 69 21 BamHI G'GATC_C Cuts at: 0 1295 1890 Size: 1295 595 BanI G'GyrC_C Cuts at: 0 145 1128 1890 Size: 145 983 762 Fragments arranged by size: 983 762 145 BbsI GAAGACnn'nnnn_ Cuts at: 0 873 882 1037 1550 1890 Size: 873 9 155 513 340 Fragments arranged by size: 873 513 340 155 9 BciVI GTATCCnnnnn_n' Cuts at: 0 450 1227 1890 Size: 450 777 663 Fragments arranged by size: 777 663 450 Bme1580I G_kGCm'C Cuts at: 0 1272 1890 Size: 1272 618 BmgBI CAC'GTC Cuts at: 0 161 798 1890 Size: 161 637 1092 Fragments arranged by size: 1092 637 161 BmrI ACTGGGnnnn_n' Cuts at: 0 208 311 1890 Size: 208 103 1579 Fragments arranged by size: 1579 208 103 BmtI G_CTAG'C Cuts at: 0 1055 1890 Size: 1055 835 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 351 1718 1890 Size: 351 1367 172 Fragments arranged by size: 1367 351 172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 534 1398 1793 1890 Size: 534 864 395 97 Fragments arranged by size: 864 534 395 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 905 1890 Size: 905 985 BsaBI GATnn'nnATC Cuts at: 0 1678 1890 Size: 1678 212 BsaHI Gr'CG_yC Cuts at: 0 851 1890 Size: 851 1039 BsaWI w'CCGG_w Cuts at: 0 1206 1890 Size: 1206 684 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 851 881 1890 Size: 851 30 1009 Fragments arranged by size: 1009 851 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 418 1890 Size: 418 1472 BseYI C'CCAG_C Cuts at: 0 469 1427 1890 Size: 469 958 463 Fragments arranged by size: 958 469 463 BsmBI CGTCTCn'nnnn_ Cuts at: 0 594 1823 1890 Size: 594 1229 67 Fragments arranged by size: 1229 594 67 Bsp1286I G_dGCh'C Cuts at: 0 1272 1890 Size: 1272 618 BspHI T'CATG_A Cuts at: 0 710 782 1890 Size: 710 72 1108 Fragments arranged by size: 1108 710 72 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1448 1591 1890 Size: 1448 143 299 Fragments arranged by size: 1448 299 143 BsrBI CCG'CTC Cuts at: 0 669 1890 Size: 669 1221 BsrDI GCAATG_nn' Cuts at: 0 581 1190 1890 Size: 581 609 700 Fragments arranged by size: 700 609 581 BsrFI r'CCGG_y Cuts at: 0 81 1890 Size: 81 1809 BssSI C'ACGA_G Cuts at: 0 325 1471 1890 Size: 325 1146 419 Fragments arranged by size: 1146 419 325 BstXI CCAn_nnnn'nTGG Cuts at: 0 546 1890 Size: 546 1344 BstYI r'GATC_y Cuts at: 0 896 1295 1890 Size: 896 399 595 Fragments arranged by size: 896 595 399 BstZ17I GTA'TAC Cuts at: 0 1088 1657 1890 Size: 1088 569 233 Fragments arranged by size: 1088 569 233 BtgI C'CryG_G Cuts at: 0 995 1890 Size: 995 895 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 582 1890 Size: 582 1308 BtsI GCAGTG_nn' Cuts at: 0 1446 1890 Size: 1446 444 DraI TTT'AAA Cuts at: 0 1727 1890 Size: 1727 163 DrdI GACnn_nn'nnGTC Cuts at: 0 1489 1890 Size: 1489 401 EaeI y'GGCC_r Cuts at: 0 803 1289 1890 Size: 803 486 601 Fragments arranged by size: 803 601 486 EarI CTCTTCn'nnn_ Cuts at: 0 946 1736 1890 Size: 946 790 154 Fragments arranged by size: 946 790 154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 265 1890 Size: 265 1625 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 534 876 970 1209 1398 1793 1890 Size: 534 342 94 239 189 395 97 Fragments arranged by size: 534 395 342 239 189 97 94 EcoO109I rG'GnC_Cy Cuts at: 0 73 110 518 1890 Size: 73 37 408 1372 Fragments arranged by size: 1372 408 73 37 FspI TGC'GCA Cuts at: 0 931 1890 Size: 931 959 HaeII r_GCGC'y Cuts at: 0 441 482 754 1890 Size: 441 41 272 1136 Fragments arranged by size: 1136 441 272 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 851 883 1027 1059 1890 Size: 851 32 144 32 831 Fragments arranged by size: 851 831 144 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1244 1890 Size: 1244 646 MscI TGG'CCA Cuts at: 0 805 1291 1890 Size: 805 486 599 Fragments arranged by size: 805 599 486 MslI CAynn'nnrTG Cuts at: 0 796 1890 Size: 796 1094 MspA1I CmG'CkG Cuts at: 0 43 469 857 1368 1890 Size: 43 426 388 511 522 Fragments arranged by size: 522 511 426 388 43 NaeI GCC'GGC Cuts at: 0 83 1890 Size: 83 1807 NcoI C'CATG_G Cuts at: 0 995 1890 Size: 995 895 NgoMIV G'CCGG_C Cuts at: 0 81 1890 Size: 81 1809 NheI G'CTAG_C Cuts at: 0 1051 1890 Size: 1051 839 NruI TCG'CGA Cuts at: 0 601 1890 Size: 601 1289 NspI r_CATG'y Cuts at: 0 1335 1890 Size: 1335 555 PfoI T'CCnGG_A Cuts at: 0 374 1890 Size: 374 1516 PpuMI rG'GwC_Cy Cuts at: 0 110 1890 Size: 110 1780 PstI C_TGCA'G Cuts at: 0 446 750 1077 1373 1890 Size: 446 304 327 296 517 Fragments arranged by size: 517 446 327 304 296 PvuII CAG'CTG Cuts at: 0 1368 1890 Size: 1368 522 SbfI CC_TGCA'GG Cuts at: 0 446 1890 Size: 446 1444 ScaI AGT'ACT Cuts at: 0 1855 1890 Size: 1855 35 SfcI C'TryA_G Cuts at: 0 442 746 1016 1073 1369 1781 1890 Size: 442 304 270 57 296 412 109 Fragments arranged by size: 442 412 304 296 270 109 57 SmlI C'TyrA_G Cuts at: 0 920 1890 Size: 920 970 SspI AAT'ATT Cuts at: 0 1869 1890 Size: 1869 21 StuI AGG'CCT Cuts at: 0 926 1890 Size: 926 964 StyI C'CwwG_G Cuts at: 0 995 1890 Size: 995 895 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 245 1890 Size: 245 1645 TatI w'GTAC_w Cuts at: 0 407 1853 1890 Size: 407 1446 37 Fragments arranged by size: 1446 407 37 ZraI GAC'GTC Cuts at: 0 852 1890 Size: 852 1038 Enzymes that do cut: AarI AatII AccI AclI AcuI AlwNI ApoI AvaI BamHI BanI BbsI BciVI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NruI NspI PfoI PpuMI PstI PvuII SbfI ScaI SfcI SmlI SspI StuI StyI TaqII TatI ZraI Enzymes that do not cut: Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bpu10I BsaI BsaAI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BsrGI BssHII BstAPI BstBI BstEII Bsu36I ClaI CspCI DraIII EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NotI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI