Restriction map for pF1KSDA1044
 (Linear) (Six-base) MAPSORT of: KIAA1044.seq  Check: 6385  from: 1  to: 1890



KIAA1044.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1448    1591    1890 
   Size:       1448     143     299

  Fragments arranged by size: 

                1448     299     143
 
AatII G_ACGT'C

Cuts at:      0     854    1890 
   Size:        854    1036
 
AccI GT'mk_AC

Cuts at:      0    1087    1656    1890 
   Size:       1087     569     234

  Fragments arranged by size: 

                1087     569     234
 
AclI AA'CG_TT

Cuts at:      0     180    1890 
   Size:        180    1710
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     876     970    1209    1890 
   Size:        876      94     239     681

  Fragments arranged by size: 

                 876     681     239      94
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1583    1890 
   Size:       1583     307
 
ApoI r'AATT_y

Cuts at:      0     288    1890 
   Size:        288    1602
 
AvaI C'yCGr_G

Cuts at:      0      69      90    1890 
   Size:         69      21    1800

  Fragments arranged by size: 

                1800      69      21
 
BamHI G'GATC_C

Cuts at:      0    1295    1890 
   Size:       1295     595
 
BanI G'GyrC_C

Cuts at:      0     145    1128    1890 
   Size:        145     983     762

  Fragments arranged by size: 

                 983     762     145
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     873     882    1037    1550    1890 
   Size:        873       9     155     513     340

  Fragments arranged by size: 

                 873     513     340     155       9
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     450    1227    1890 
   Size:        450     777     663

  Fragments arranged by size: 

                 777     663     450
 
Bme1580I G_kGCm'C

Cuts at:      0    1272    1890 
   Size:       1272     618
 
BmgBI CAC'GTC

Cuts at:      0     161     798    1890 
   Size:        161     637    1092

  Fragments arranged by size: 

                1092     637     161
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     208     311    1890 
   Size:        208     103    1579

  Fragments arranged by size: 

                1579     208     103
 
BmtI G_CTAG'C

Cuts at:      0    1055    1890 
   Size:       1055     835
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     351    1718    1890 
   Size:        351    1367     172

  Fragments arranged by size: 

                1367     351     172
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     534    1398    1793    1890 
   Size:        534     864     395      97

  Fragments arranged by size: 

                 864     534     395      97
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     905    1890 
   Size:        905     985
 
BsaBI GATnn'nnATC

Cuts at:      0    1678    1890 
   Size:       1678     212
 
BsaHI Gr'CG_yC

Cuts at:      0     851    1890 
   Size:        851    1039
 
BsaWI w'CCGG_w

Cuts at:      0    1206    1890 
   Size:       1206     684
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     851     881    1890 
   Size:        851      30    1009

  Fragments arranged by size: 

                1009     851      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     418    1890 
   Size:        418    1472
 
BseYI C'CCAG_C

Cuts at:      0     469    1427    1890 
   Size:        469     958     463

  Fragments arranged by size: 

                 958     469     463
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     594    1823    1890 
   Size:        594    1229      67

  Fragments arranged by size: 

                1229     594      67
 
Bsp1286I G_dGCh'C

Cuts at:      0    1272    1890 
   Size:       1272     618
 
BspHI T'CATG_A

Cuts at:      0     710     782    1890 
   Size:        710      72    1108

  Fragments arranged by size: 

                1108     710      72
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1448    1591    1890 
   Size:       1448     143     299

  Fragments arranged by size: 

                1448     299     143
 
BsrBI CCG'CTC

Cuts at:      0     669    1890 
   Size:        669    1221
 
BsrDI GCAATG_nn'

Cuts at:      0     581    1190    1890 
   Size:        581     609     700

  Fragments arranged by size: 

                 700     609     581
 
BsrFI r'CCGG_y

Cuts at:      0      81    1890 
   Size:         81    1809
 
BssSI C'ACGA_G

Cuts at:      0     325    1471    1890 
   Size:        325    1146     419

  Fragments arranged by size: 

                1146     419     325
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     546    1890 
   Size:        546    1344
 
BstYI r'GATC_y

Cuts at:      0     896    1295    1890 
   Size:        896     399     595

  Fragments arranged by size: 

                 896     595     399
 
BstZ17I GTA'TAC

Cuts at:      0    1088    1657    1890 
   Size:       1088     569     233

  Fragments arranged by size: 

                1088     569     233
 
BtgI C'CryG_G

Cuts at:      0     995    1890 
   Size:        995     895
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     582    1890 
   Size:        582    1308
 
BtsI GCAGTG_nn'

Cuts at:      0    1446    1890 
   Size:       1446     444
 
DraI TTT'AAA

Cuts at:      0    1727    1890 
   Size:       1727     163
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1489    1890 
   Size:       1489     401
 
EaeI y'GGCC_r

Cuts at:      0     803    1289    1890 
   Size:        803     486     601

  Fragments arranged by size: 

                 803     601     486
 
EarI CTCTTCn'nnn_

Cuts at:      0     946    1736    1890 
   Size:        946     790     154

  Fragments arranged by size: 

                 946     790     154
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     265    1890 
   Size:        265    1625
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     534     876     970    1209    1398    1793    1890 
   Size:        534     342      94     239     189     395      97

  Fragments arranged by size: 

                 534     395     342     239     189      97      94
 
EcoO109I rG'GnC_Cy

Cuts at:      0      73     110     518    1890 
   Size:         73      37     408    1372

  Fragments arranged by size: 

                1372     408      73      37
 
FspI TGC'GCA

Cuts at:      0     931    1890 
   Size:        931     959
 
HaeII r_GCGC'y

Cuts at:      0     441     482     754    1890 
   Size:        441      41     272    1136

  Fragments arranged by size: 

                1136     441     272      41
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     851     883    1027    1059    1890 
   Size:        851      32     144      32     831

  Fragments arranged by size: 

                 851     831     144      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1244    1890 
   Size:       1244     646
 
MscI TGG'CCA

Cuts at:      0     805    1291    1890 
   Size:        805     486     599

  Fragments arranged by size: 

                 805     599     486
 
MslI CAynn'nnrTG

Cuts at:      0     796    1890 
   Size:        796    1094
 
MspA1I CmG'CkG

Cuts at:      0      43     469     857    1368    1890 
   Size:         43     426     388     511     522

  Fragments arranged by size: 

                 522     511     426     388      43
 
NaeI GCC'GGC

Cuts at:      0      83    1890 
   Size:         83    1807
 
NcoI C'CATG_G

Cuts at:      0     995    1890 
   Size:        995     895
 
NgoMIV G'CCGG_C

Cuts at:      0      81    1890 
   Size:         81    1809
 
NheI G'CTAG_C

Cuts at:      0    1051    1890 
   Size:       1051     839
 
NruI TCG'CGA

Cuts at:      0     601    1890 
   Size:        601    1289
 
NspI r_CATG'y

Cuts at:      0    1335    1890 
   Size:       1335     555
 
PfoI T'CCnGG_A

Cuts at:      0     374    1890 
   Size:        374    1516
 
PpuMI rG'GwC_Cy

Cuts at:      0     110    1890 
   Size:        110    1780
 
PstI C_TGCA'G

Cuts at:      0     446     750    1077    1373    1890 
   Size:        446     304     327     296     517

  Fragments arranged by size: 

                 517     446     327     304     296
 
PvuII CAG'CTG

Cuts at:      0    1368    1890 
   Size:       1368     522
 
SbfI CC_TGCA'GG

Cuts at:      0     446    1890 
   Size:        446    1444
 
ScaI AGT'ACT

Cuts at:      0    1855    1890 
   Size:       1855      35
 
SfcI C'TryA_G

Cuts at:      0     442     746    1016    1073    1369    1781    1890 
   Size:        442     304     270      57     296     412     109

  Fragments arranged by size: 

                 442     412     304     296     270     109      57
 
SmlI C'TyrA_G

Cuts at:      0     920    1890 
   Size:        920     970
 
SspI AAT'ATT

Cuts at:      0    1869    1890 
   Size:       1869      21
 
StuI AGG'CCT

Cuts at:      0     926    1890 
   Size:        926     964
 
StyI C'CwwG_G

Cuts at:      0     995    1890 
   Size:        995     895
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     245    1890 
   Size:        245    1645
 
TatI w'GTAC_w

Cuts at:      0     407    1853    1890 
   Size:        407    1446      37

  Fragments arranged by size: 

                1446     407      37
 
ZraI GAC'GTC

Cuts at:      0     852    1890 
   Size:        852    1038
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI    AlwNI     ApoI     AvaI
    BamHI     BanI     BbsI    BciVI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NheI     NruI     NspI     PfoI    PpuMI     PstI    PvuII     SbfI
     ScaI     SfcI     SmlI     SspI     StuI     StyI    TaqII     TatI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI   Bpu10I     BsaI    BsaAI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BsrGI   BssHII   BstAPI    BstBI   BstEII   Bsu36I
     ClaI    CspCI   DraIII     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NdeI     NotI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI
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