Restriction map for
pF1KSDA1044
(Linear) (Six-base) MAPSORT of: KIAA1044.seq Check: 6385 from: 1 to: 1890
KIAA1044.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1448 1591 1890
Size: 1448 143 299
Fragments arranged by size:
1448 299 143
AatII G_ACGT'C
Cuts at: 0 854 1890
Size: 854 1036
AccI GT'mk_AC
Cuts at: 0 1087 1656 1890
Size: 1087 569 234
Fragments arranged by size:
1087 569 234
AclI AA'CG_TT
Cuts at: 0 180 1890
Size: 180 1710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 876 970 1209 1890
Size: 876 94 239 681
Fragments arranged by size:
876 681 239 94
AlwNI CAG_nnn'CTG
Cuts at: 0 1583 1890
Size: 1583 307
ApoI r'AATT_y
Cuts at: 0 288 1890
Size: 288 1602
AvaI C'yCGr_G
Cuts at: 0 69 90 1890
Size: 69 21 1800
Fragments arranged by size:
1800 69 21
BamHI G'GATC_C
Cuts at: 0 1295 1890
Size: 1295 595
BanI G'GyrC_C
Cuts at: 0 145 1128 1890
Size: 145 983 762
Fragments arranged by size:
983 762 145
BbsI GAAGACnn'nnnn_
Cuts at: 0 873 882 1037 1550 1890
Size: 873 9 155 513 340
Fragments arranged by size:
873 513 340 155 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 450 1227 1890
Size: 450 777 663
Fragments arranged by size:
777 663 450
Bme1580I G_kGCm'C
Cuts at: 0 1272 1890
Size: 1272 618
BmgBI CAC'GTC
Cuts at: 0 161 798 1890
Size: 161 637 1092
Fragments arranged by size:
1092 637 161
BmrI ACTGGGnnnn_n'
Cuts at: 0 208 311 1890
Size: 208 103 1579
Fragments arranged by size:
1579 208 103
BmtI G_CTAG'C
Cuts at: 0 1055 1890
Size: 1055 835
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 351 1718 1890
Size: 351 1367 172
Fragments arranged by size:
1367 351 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 1398 1793 1890
Size: 534 864 395 97
Fragments arranged by size:
864 534 395 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1890
Size: 905 985
BsaBI GATnn'nnATC
Cuts at: 0 1678 1890
Size: 1678 212
BsaHI Gr'CG_yC
Cuts at: 0 851 1890
Size: 851 1039
BsaWI w'CCGG_w
Cuts at: 0 1206 1890
Size: 1206 684
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 851 881 1890
Size: 851 30 1009
Fragments arranged by size:
1009 851 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 418 1890
Size: 418 1472
BseYI C'CCAG_C
Cuts at: 0 469 1427 1890
Size: 469 958 463
Fragments arranged by size:
958 469 463
BsmBI CGTCTCn'nnnn_
Cuts at: 0 594 1823 1890
Size: 594 1229 67
Fragments arranged by size:
1229 594 67
Bsp1286I G_dGCh'C
Cuts at: 0 1272 1890
Size: 1272 618
BspHI T'CATG_A
Cuts at: 0 710 782 1890
Size: 710 72 1108
Fragments arranged by size:
1108 710 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1448 1591 1890
Size: 1448 143 299
Fragments arranged by size:
1448 299 143
BsrBI CCG'CTC
Cuts at: 0 669 1890
Size: 669 1221
BsrDI GCAATG_nn'
Cuts at: 0 581 1190 1890
Size: 581 609 700
Fragments arranged by size:
700 609 581
BsrFI r'CCGG_y
Cuts at: 0 81 1890
Size: 81 1809
BssSI C'ACGA_G
Cuts at: 0 325 1471 1890
Size: 325 1146 419
Fragments arranged by size:
1146 419 325
BstXI CCAn_nnnn'nTGG
Cuts at: 0 546 1890
Size: 546 1344
BstYI r'GATC_y
Cuts at: 0 896 1295 1890
Size: 896 399 595
Fragments arranged by size:
896 595 399
BstZ17I GTA'TAC
Cuts at: 0 1088 1657 1890
Size: 1088 569 233
Fragments arranged by size:
1088 569 233
BtgI C'CryG_G
Cuts at: 0 995 1890
Size: 995 895
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 582 1890
Size: 582 1308
BtsI GCAGTG_nn'
Cuts at: 0 1446 1890
Size: 1446 444
DraI TTT'AAA
Cuts at: 0 1727 1890
Size: 1727 163
DrdI GACnn_nn'nnGTC
Cuts at: 0 1489 1890
Size: 1489 401
EaeI y'GGCC_r
Cuts at: 0 803 1289 1890
Size: 803 486 601
Fragments arranged by size:
803 601 486
EarI CTCTTCn'nnn_
Cuts at: 0 946 1736 1890
Size: 946 790 154
Fragments arranged by size:
946 790 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 1890
Size: 265 1625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 876 970 1209 1398 1793 1890
Size: 534 342 94 239 189 395 97
Fragments arranged by size:
534 395 342 239 189 97 94
EcoO109I rG'GnC_Cy
Cuts at: 0 73 110 518 1890
Size: 73 37 408 1372
Fragments arranged by size:
1372 408 73 37
FspI TGC'GCA
Cuts at: 0 931 1890
Size: 931 959
HaeII r_GCGC'y
Cuts at: 0 441 482 754 1890
Size: 441 41 272 1136
Fragments arranged by size:
1136 441 272 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 851 883 1027 1059 1890
Size: 851 32 144 32 831
Fragments arranged by size:
851 831 144 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1244 1890
Size: 1244 646
MscI TGG'CCA
Cuts at: 0 805 1291 1890
Size: 805 486 599
Fragments arranged by size:
805 599 486
MslI CAynn'nnrTG
Cuts at: 0 796 1890
Size: 796 1094
MspA1I CmG'CkG
Cuts at: 0 43 469 857 1368 1890
Size: 43 426 388 511 522
Fragments arranged by size:
522 511 426 388 43
NaeI GCC'GGC
Cuts at: 0 83 1890
Size: 83 1807
NcoI C'CATG_G
Cuts at: 0 995 1890
Size: 995 895
NgoMIV G'CCGG_C
Cuts at: 0 81 1890
Size: 81 1809
NheI G'CTAG_C
Cuts at: 0 1051 1890
Size: 1051 839
NruI TCG'CGA
Cuts at: 0 601 1890
Size: 601 1289
NspI r_CATG'y
Cuts at: 0 1335 1890
Size: 1335 555
PfoI T'CCnGG_A
Cuts at: 0 374 1890
Size: 374 1516
PpuMI rG'GwC_Cy
Cuts at: 0 110 1890
Size: 110 1780
PstI C_TGCA'G
Cuts at: 0 446 750 1077 1373 1890
Size: 446 304 327 296 517
Fragments arranged by size:
517 446 327 304 296
PvuII CAG'CTG
Cuts at: 0 1368 1890
Size: 1368 522
SbfI CC_TGCA'GG
Cuts at: 0 446 1890
Size: 446 1444
ScaI AGT'ACT
Cuts at: 0 1855 1890
Size: 1855 35
SfcI C'TryA_G
Cuts at: 0 442 746 1016 1073 1369 1781 1890
Size: 442 304 270 57 296 412 109
Fragments arranged by size:
442 412 304 296 270 109 57
SmlI C'TyrA_G
Cuts at: 0 920 1890
Size: 920 970
SspI AAT'ATT
Cuts at: 0 1869 1890
Size: 1869 21
StuI AGG'CCT
Cuts at: 0 926 1890
Size: 926 964
StyI C'CwwG_G
Cuts at: 0 995 1890
Size: 995 895
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 245 1890
Size: 245 1645
TatI w'GTAC_w
Cuts at: 0 407 1853 1890
Size: 407 1446 37
Fragments arranged by size:
1446 407 37
ZraI GAC'GTC
Cuts at: 0 852 1890
Size: 852 1038
Enzymes that do cut:
AarI AatII AccI AclI AcuI AlwNI ApoI AvaI
BamHI BanI BbsI BciVI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EarI EciI Eco57MI EcoO109I FspI HaeII
Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NheI NruI NspI PfoI PpuMI PstI PvuII SbfI
ScaI SfcI SmlI SspI StuI StyI TaqII TatI
ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanII BbeI BbvCI BcgI BclI BfrBI BglI BglII
BlpI Bpu10I BsaI BsaAI BsgI BsiEI BsiHKAI BsiWI
BsmI BspEI BsrGI BssHII BstAPI BstBI BstEII Bsu36I
ClaI CspCI DraIII EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NotI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI