Restriction map for
pF1KA1040
(Linear) (Six-base) MAPSORT of: KIAA1040.seq Check: 1292 from: 1 to: 5154
KIAA1040.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 1579 5154
Size: 1579 3575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 1460 1574 2098 2773 4758 5048 5154
Size: 566 894 114 524 675 1985 290 106
Fragments arranged by size:
1985 894 675 566 524 290 114 106
AflII C'TTAA_G
Cuts at: 0 364 4969 5154
Size: 364 4605 185
Fragments arranged by size:
4605 364 185
AleI CACnn'nnGTG
Cuts at: 0 78 5154
Size: 78 5076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 952 5154
Size: 952 4202
AlwNI CAG_nnn'CTG
Cuts at: 0 496 1085 2583 2676 5154
Size: 496 589 1498 93 2478
Fragments arranged by size:
2478 1498 589 496 93
ApaI G_GGCC'C
Cuts at: 0 3605 5154
Size: 3605 1549
ApoI r'AATT_y
Cuts at: 0 91 164 796 1021 2429 3515 4108 4456
Size: 91 73 632 225 1408 1086 593 348
Cuts at: 4456 4633 4732 5154
Size: 177 99 422
Fragments arranged by size:
1408 1086 632 593 422 348 225 177
99 91 73
AseI AT'TA_AT
Cuts at: 0 2627 5154
Size: 2627 2527
AvaI C'yCGr_G
Cuts at: 0 19 5154
Size: 19 5135
BamHI G'GATC_C
Cuts at: 0 3105 5154
Size: 3105 2049
BanI G'GyrC_C
Cuts at: 0 7 2158 5154
Size: 7 2151 2996
Fragments arranged by size:
2996 2151 7
BanII G_rGCy'C
Cuts at: 0 3605 4672 5154
Size: 3605 1067 482
Fragments arranged by size:
3605 1067 482
BbsI GAAGACnn'nnnn_
Cuts at: 0 1672 1785 2779 5154
Size: 1672 113 994 2375
Fragments arranged by size:
2375 1672 994 113
BciVI GTATCCnnnnn_n'
Cuts at: 0 1346 4082 4401 5154
Size: 1346 2736 319 753
Fragments arranged by size:
2736 1346 753 319
BclI T'GATC_A
Cuts at: 0 2748 5154
Size: 2748 2406
BfrBI ATG'CAT
Cuts at: 0 646 3946 5048 5154
Size: 646 3300 1102 106
Fragments arranged by size:
3300 1102 646 106
BglI GCCn_nnn'nGGC
Cuts at: 0 2709 5154
Size: 2709 2445
BglII A'GATC_T
Cuts at: 0 610 5154
Size: 610 4544
Bme1580I G_kGCm'C
Cuts at: 0 3605 5013 5154
Size: 3605 1408 141
Fragments arranged by size:
3605 1408 141
BmgBI CAC'GTC
Cuts at: 0 3344 5154
Size: 3344 1810
BmrI ACTGGGnnnn_n'
Cuts at: 0 1186 1792 2186 5154
Size: 1186 606 394 2968
Fragments arranged by size:
2968 1186 606 394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 207 5154
Size: 207 4947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 97 878 3302 3383 4294 4508 4747
Size: 61 36 781 2424 81 911 214 239
Cuts at: 4747 5154
Size: 407
Fragments arranged by size:
2424 911 781 407 239 214 81 61
36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1982 2482 2632 4781 5154
Size: 769 1213 500 150 2149 373
Fragments arranged by size:
2149 1213 769 500 373 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 2433 5154
Size: 2433 2721
BsaBI GATnn'nnATC
Cuts at: 0 3713 5154
Size: 3713 1441
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1682 1712 3756 3786 5154
Size: 1682 30 2044 30 1368
Fragments arranged by size:
2044 1682 1368 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 799 4390 5154
Size: 799 3591 764
Fragments arranged by size:
3591 799 764
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 1111 1673 3029 3152 5063 5154
Size: 134 977 562 1356 123 1911 91
Fragments arranged by size:
1911 1356 977 562 134 123 91
BsiHKAI G_wGCw'C
Cuts at: 0 4310 5154
Size: 4310 844
BsmI GAATG_Cn'
Cuts at: 0 1140 1462 1668 2665 2776 3183 5050 5154
Size: 1140 322 206 997 111 407 1867 104
Fragments arranged by size:
1867 1140 997 407 322 206 111 104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3334 5154
Size: 3334 1820
Bsp1286I G_dGCh'C
Cuts at: 0 3605 4310 4672 5013 5154
Size: 3605 705 362 341 141
Fragments arranged by size:
3605 705 362 341 141
BspHI T'CATG_A
Cuts at: 0 414 5154
Size: 414 4740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 765 2848 5154
Size: 765 2083 2306
Fragments arranged by size:
2306 2083 765
BsrDI GCAATG_nn'
Cuts at: 0 2061 2342 3337 4154 4602 5154
Size: 2061 281 995 817 448 552
Fragments arranged by size:
2061 995 817 552 448 281
BsrFI r'CCGG_y
Cuts at: 0 4194 5154
Size: 4194 960
BsrGI T'GTAC_A
Cuts at: 0 201 5154
Size: 201 4953
BssHII G'CGCG_C
Cuts at: 0 63 5154
Size: 63 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2676 5154
Size: 2676 2478
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1458 5154
Size: 1458 3696
BstYI r'GATC_y
Cuts at: 0 610 663 1180 3105 3374 3754 4178 4843
Size: 610 53 517 1925 269 380 424 665
Cuts at: 4843 5154
Size: 311
Fragments arranged by size:
1925 665 610 517 424 380 311 269
53
Bsu36I CC'TnA_GG
Cuts at: 0 4628 5154
Size: 4628 526
BtgI C'CryG_G
Cuts at: 0 2067 5154
Size: 2067 3087
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4846 5154
Size: 4846 308
BtsI GCAGTG_nn'
Cuts at: 0 1389 1874 1976 2219 2290 3254 4295 5154
Size: 1389 485 102 243 71 964 1041 859
Fragments arranged by size:
1389 1041 964 859 485 243 102 71
DraI TTT'AAA
Cuts at: 0 1745 3026 4838 4940 5154
Size: 1745 1281 1812 102 214
Fragments arranged by size:
1812 1745 1281 214 102
EaeI y'GGCC_r
Cuts at: 0 2451 2710 3031 4471 5154
Size: 2451 259 321 1440 683
Fragments arranged by size:
2451 1440 683 321 259
EarI CTCTTCn'nnn_
Cuts at: 0 2117 2975 3002 3121 4907 5154
Size: 2117 858 27 119 1786 247
Fragments arranged by size:
2117 1786 858 247 119 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4213 5154
Size: 4213 941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 97 566 878 1460 1574 2098 2773
Size: 61 36 469 312 582 114 524 675
Cuts at: 2773 3302 3383 4294 4508 4747 4758 5048 5154
Size: 529 81 911 214 239 11 290 106
Fragments arranged by size:
911 675 582 529 524 469 312 290
239 214 114 106 81 61 36 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4799 5154
Size: 4799 355
EcoO109I rG'GnC_Cy
Cuts at: 0 2188 3601 5154
Size: 2188 1413 1553
Fragments arranged by size:
2188 1553 1413
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3234 4024 5154
Size: 3234 790 1130
Fragments arranged by size:
3234 1130 790
FspI TGC'GCA
Cuts at: 0 3164 5154
Size: 3164 1990
FspAI rTGC'GCAy
Cuts at: 0 3164 5154
Size: 3164 1990
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1585 1617 1920 1952 2272 2304 2439 2471
Size: 1585 32 303 32 320 32 135 32
Cuts at: 2471 4056 4088 4519 4551 5154
Size: 1585 32 431 32 603
Fragments arranged by size:
1585 1585 603 431 320 303 135 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1580 1604 1978 5154
Size: 1580 24 374 3176
Fragments arranged by size:
3176 1580 374 24
HindIII A'AGCT_T
Cuts at: 0 2513 3504 5154
Size: 2513 991 1650
Fragments arranged by size:
2513 1650 991
MfeI C'AATT_G
Cuts at: 0 143 3155 5154
Size: 143 3012 1999
Fragments arranged by size:
3012 1999 143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1391 1453 1916 4206 5154
Size: 1391 62 463 2290 948
Fragments arranged by size:
2290 1391 948 463 62
MscI TGG'CCA
Cuts at: 0 2453 2712 4473 5154
Size: 2453 259 1761 681
Fragments arranged by size:
2453 1761 681 259
MslI CAynn'nnrTG
Cuts at: 0 78 1456 1501 2066 2781 5154
Size: 78 1378 45 565 715 2373
Fragments arranged by size:
2373 1378 715 565 78 45
MspA1I CmG'CkG
Cuts at: 0 310 493 4140 5154
Size: 310 183 3647 1014
Fragments arranged by size:
3647 1014 310 183
NcoI C'CATG_G
Cuts at: 0 2067 5154
Size: 2067 3087
NsiI A_TGCA'T
Cuts at: 0 648 3948 5050 5154
Size: 648 3300 1102 104
Fragments arranged by size:
3300 1102 648 104
NspI r_CATG'y
Cuts at: 0 330 933 4062 4444 5154
Size: 330 603 3129 382 710
Fragments arranged by size:
3129 710 603 382 330
PflMI CCAn_nnn'nTGG
Cuts at: 0 4095 4132 5154
Size: 4095 37 1022
Fragments arranged by size:
4095 1022 37
PfoI T'CCnGG_A
Cuts at: 0 659 3509 5154
Size: 659 2850 1645
Fragments arranged by size:
2850 1645 659
PpuMI rG'GwC_Cy
Cuts at: 0 2188 5154
Size: 2188 2966
PshAI GACnn'nnGTC
Cuts at: 0 279 5154
Size: 279 4875
PsiI TTA'TAA
Cuts at: 0 1813 5154
Size: 1813 3341
PspOMI G'GGCC_C
Cuts at: 0 3601 5154
Size: 3601 1553
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 353 385 566 598 938 970 5154
Size: 353 32 181 32 340 32 4184
Fragments arranged by size:
4184 353 340 181 32 32 32
PstI C_TGCA'G
Cuts at: 0 309 1881 2588 2681 3261 3404 5154
Size: 309 1572 707 93 580 143 1750
Fragments arranged by size:
1750 1572 707 580 309 143 93
PvuII CAG'CTG
Cuts at: 0 310 493 4140 5154
Size: 310 183 3647 1014
Fragments arranged by size:
3647 1014 310 183
SalI G'TCGA_C
Cuts at: 0 1578 5154
Size: 1578 3576
ScaI AGT'ACT
Cuts at: 0 584 3765 4953 5154
Size: 584 3181 1188 201
Fragments arranged by size:
3181 1188 584 201
SexAI A'CCwGG_T
Cuts at: 0 3739 5154
Size: 3739 1415
SfcI C'TryA_G
Cuts at: 0 305 500 1877 2180 2584 2677 3257 3400
Size: 305 195 1377 303 404 93 580 143
Cuts at: 3400 3569 5154
Size: 169 1585
Fragments arranged by size:
1585 1377 580 404 305 303 195 169
143 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2709 5154
Size: 2709 2445
SgrAI Cr'CCGG_yG
Cuts at: 0 4194 5154
Size: 4194 960
SmlI C'TyrA_G
Cuts at: 0 364 748 1997 2461 2647 4796 4969 5154
Size: 364 384 1249 464 186 2149 173 185
Fragments arranged by size:
2149 1249 464 384 364 186 185 173
SpeI A'CTAG_T
Cuts at: 0 5094 5154
Size: 5094 60
SphI G_CATG'C
Cuts at: 0 933 4444 5154
Size: 933 3511 710
Fragments arranged by size:
3511 933 710
SspI AAT'ATT
Cuts at: 0 477 1740 2625 2892 2925 3294 4317 4566
Size: 477 1263 885 267 33 369 1023 249
Cuts at: 4566 5154
Size: 588
Fragments arranged by size:
1263 1023 885 588 477 369 267 249
33
StyI C'CwwG_G
Cuts at: 0 230 1148 2067 4906 5154
Size: 230 918 919 2839 248
Fragments arranged by size:
2839 919 918 248 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3079 3699 5154
Size: 3079 620 1455
Fragments arranged by size:
3079 1455 620
TatI w'GTAC_w
Cuts at: 0 201 582 2194 2827 3763 4005 4854 4951
Size: 201 381 1612 633 936 242 849 97
Cuts at: 4951 5154
Size: 203
Fragments arranged by size:
1612 936 849 633 381 242 203 201
97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1682 1714 5154
Size: 1682 32 3440
Fragments arranged by size:
3440 1682 32
XbaI T'CTAG_A
Cuts at: 0 2328 5154
Size: 2328 2826
XmnI GAAnn'nnTTC
Cuts at: 0 91 3505 3639 4499 5154
Size: 91 3414 134 860 655
Fragments arranged by size:
3414 860 655 134 91
Enzymes that do cut:
AccI AcuI AflII AleI AlfI AlwNI ApaI ApoI
AseI AvaI BamHI BanI BanII BbsI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaBI BsaXI BseRI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssHII
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI
FspAI Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NsiI NspI PflMI PfoI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuII SalI ScaI SexAI
SfcI SfiI SgrAI SmlI SpeI SphI SspI StyI
TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AloI ApaLI AscI AsiSI AvrII BaeI BbeI BbvCI
BcgI BlpI BmtI Bpu10I BsaAI BsaHI BsaWI BseYI
BsiEI BsiWI BspEI BsrBI BssSI BstBI BstEII BstZ17I
ClaI CspCI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FseI HaeII HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PspXI PvuI RsrII SacI SacII
SanDI SapI SbfI SfoI SgfI SmaI SnaBI SrfI
StuI SwaI Tth111I XcmI XhoI XmaI ZraI