Restriction map for
pF1KSDA1029
(Linear) (Six-base) MAPSORT of: KIAA1029.seq Check: 7882 from: 1 to: 2709
KIAA1029.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 662 2709
Size: 662 2047
Acc65I G'GTAC_C
Cuts at: 0 69 1344 2709
Size: 69 1275 1365
Fragments arranged by size:
1365 1275 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1081 2709
Size: 1081 1628
AfeI AGC'GCT
Cuts at: 0 2203 2709
Size: 2203 506
AflIII A'CryG_T
Cuts at: 0 2576 2709
Size: 2576 133
AhdI GACnn_n'nnGTC
Cuts at: 0 1047 2709
Size: 1047 1662
AleI CACnn'nnGTG
Cuts at: 0 601 1906 2709
Size: 601 1305 803
Fragments arranged by size:
1305 803 601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1919 2709
Size: 1919 790
AlwNI CAG_nnn'CTG
Cuts at: 0 324 388 2709
Size: 324 64 2321
Fragments arranged by size:
2321 324 64
ApaI G_GGCC'C
Cuts at: 0 2119 2709
Size: 2119 590
ApoI r'AATT_y
Cuts at: 0 348 2709
Size: 348 2361
AvaI C'yCGr_G
Cuts at: 0 1576 1967 2112 2709
Size: 1576 391 145 597
Fragments arranged by size:
1576 597 391 145
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1336 1369 2709
Size: 1336 33 1340
Fragments arranged by size:
1340 1336 33
BamHI G'GATC_C
Cuts at: 0 731 2709
Size: 731 1978
BanI G'GyrC_C
Cuts at: 0 35 69 432 738 748 1344 1794 2326
Size: 35 34 363 306 10 596 450 532
Cuts at: 2326 2361 2709
Size: 35 348
Fragments arranged by size:
596 532 450 363 348 306 35 35
34 10
BanII G_rGCy'C
Cuts at: 0 772 844 1259 1448 1676 1818 1915 2113
Size: 772 72 415 189 228 142 97 198
Cuts at: 2113 2119 2479 2521 2709
Size: 6 360 42 188
Fragments arranged by size:
772 415 360 228 198 189 188 142
97 72 42 6
BbeI G_GCGC'C
Cuts at: 0 742 2330 2365 2709
Size: 742 1588 35 344
Fragments arranged by size:
1588 742 344 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1043 1484 2709
Size: 1043 441 1225
Fragments arranged by size:
1225 1043 441
BbvCI CC'TCA_GC
Cuts at: 0 179 440 2709
Size: 179 261 2269
Fragments arranged by size:
2269 261 179
BciVI GTATCCnnnnn_n'
Cuts at: 0 1040 2709
Size: 1040 1669
BglI GCCn_nnn'nGGC
Cuts at: 0 988 1579 1978 2099 2538 2709
Size: 988 591 399 121 439 171
Fragments arranged by size:
988 591 439 399 171 121
BlpI GC'TnA_GC
Cuts at: 0 1442 2709
Size: 1442 1267
Bme1580I G_kGCm'C
Cuts at: 0 751 1799 2119 2709
Size: 751 1048 320 590
Fragments arranged by size:
1048 751 590 320
BmgBI CAC'GTC
Cuts at: 0 2577 2709
Size: 2577 132
BmrI ACTGGGnnnn_n'
Cuts at: 0 221 818 1276 2709
Size: 221 597 458 1433
Fragments arranged by size:
1433 597 458 221
BmtI G_CTAG'C
Cuts at: 0 26 2709
Size: 26 2683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1025 1374 1462 2709
Size: 27 998 349 88 1247
Fragments arranged by size:
1247 998 349 88 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 158 470 495 522 1120 1935 2597
Size: 61 97 312 25 27 598 815 662
Cuts at: 2597 2709
Size: 112
Fragments arranged by size:
815 662 598 312 112 97 61 27
25
Bpu10I CC'TnA_GC
Cuts at: 0 179 440 2024 2709
Size: 179 261 1584 685
Fragments arranged by size:
1584 685 261 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 2653 2709
Size: 1351 1302 56
Fragments arranged by size:
1351 1302 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 961 1010 1752 1967 2221 2709
Size: 961 49 742 215 254 488
Fragments arranged by size:
961 742 488 254 215 49
BsaBI GATnn'nnATC
Cuts at: 0 287 2709
Size: 287 2422
BsaHI Gr'CG_yC
Cuts at: 0 739 927 2065 2327 2362 2709
Size: 739 188 1138 262 35 347
Fragments arranged by size:
1138 739 347 262 188 35
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 73 457 780 998 1117 1264 1267
Size: 31 42 384 323 218 119 147 3
Cuts at: 1267 1351 1845 2709
Size: 84 494 864
Fragments arranged by size:
864 494 384 323 218 147 119 84
42 31 3
BseYI C'CCAG_C
Cuts at: 0 149 192 400 532 546 815 1033 1605
Size: 149 43 208 132 14 269 218 572
Cuts at: 1605 1777 2086 2102 2245 2709
Size: 172 309 16 143 464
Fragments arranged by size:
572 464 309 269 218 208 172 149
143 132 43 16 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 932 2487 2685 2709
Size: 109 823 1555 198 24
Fragments arranged by size:
1555 823 198 109 24
BsiEI CG_ry'CG
Cuts at: 0 2320 2709
Size: 2320 389
BsiHKAI G_wGCw'C
Cuts at: 0 1448 2709
Size: 1448 1261
BsmI GAATG_Cn'
Cuts at: 0 102 1094 2709
Size: 102 992 1615
Fragments arranged by size:
1615 992 102
BsmBI CGTCTCn'nnnn_
Cuts at: 0 777 918 2709
Size: 777 141 1791
Fragments arranged by size:
1791 777 141
Bsp1286I G_dGCh'C
Cuts at: 0 751 772 844 1259 1448 1676 1799 1818
Size: 751 21 72 415 189 228 123 19
Cuts at: 1818 1915 2113 2119 2479 2521 2709
Size: 97 198 6 360 42 188
Fragments arranged by size:
751 415 360 228 198 189 188 123
97 72 42 21 19 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1652 1811 2709
Size: 1652 159 898
Fragments arranged by size:
1652 898 159
BsrBI CCG'CTC
Cuts at: 0 2558 2709
Size: 2558 151
BsrFI r'CCGG_y
Cuts at: 0 162 300 435 2488 2709
Size: 162 138 135 2053 221
Fragments arranged by size:
2053 221 162 138 135
BssSI C'ACGA_G
Cuts at: 0 1307 2190 2709
Size: 1307 883 519
Fragments arranged by size:
1307 883 519
BstEII G'GTnAC_C
Cuts at: 0 1164 2709
Size: 1164 1545
BstXI CCAn_nnnn'nTGG
Cuts at: 0 759 1785 2709
Size: 759 1026 924
Fragments arranged by size:
1026 924 759
BstYI r'GATC_y
Cuts at: 0 731 1188 2709
Size: 731 457 1521
Fragments arranged by size:
1521 731 457
BtgI C'CryG_G
Cuts at: 0 878 1325 1635 2119 2314 2338 2470 2564
Size: 878 447 310 484 195 24 132 94
Cuts at: 2564 2643 2709
Size: 79 66
Fragments arranged by size:
878 484 447 310 195 132 94 79
66 24
BtsI GCAGTG_nn'
Cuts at: 0 103 944 1901 2709
Size: 103 841 957 808
Fragments arranged by size:
957 841 808 103
DraIII CAC_nnn'GTG
Cuts at: 0 410 1309 2709
Size: 410 899 1400
Fragments arranged by size:
1400 899 410
EaeI y'GGCC_r
Cuts at: 0 50 686 716 847 1881 2709
Size: 50 636 30 131 1034 828
Fragments arranged by size:
1034 828 636 131 50 30
EarI CTCTTCn'nnn_
Cuts at: 0 59 2709
Size: 59 2650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 260 1348 2709
Size: 260 1088 1361
Fragments arranged by size:
1361 1088 260
EcoICRI GAG'CTC
Cuts at: 0 1446 2709
Size: 1446 1263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 158 470 495 522 1081 1120 1935
Size: 61 97 312 25 27 559 39 815
Cuts at: 1935 2597 2709
Size: 662 112
Fragments arranged by size:
815 662 559 312 112 97 61 39
27 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 999 1420 1807 2709
Size: 43 956 421 387 902
Fragments arranged by size:
956 902 421 387 43
EcoO109I rG'GnC_Cy
Cuts at: 0 385 542 804 1580 1659 1980 1988 2540
Size: 385 157 262 776 79 321 8 552
Cuts at: 2540 2709
Size: 169
Fragments arranged by size:
776 552 385 321 262 169 157 79
8
EcoRI G'AATT_C
Cuts at: 0 348 2709
Size: 348 2361
HaeII r_GCGC'y
Cuts at: 0 742 2205 2266 2309 2330 2365 2709
Size: 742 1463 61 43 21 35 344
Fragments arranged by size:
1463 742 344 61 43 35 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 547 579 958 990 1033 1065
Size: 463 32 52 32 379 32 43 32
Cuts at: 1065 1158 1190 2007 2039 2709
Size: 93 32 817 32 670
Fragments arranged by size:
817 670 463 379 93 52 43 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 207 708 2709
Size: 207 501 2001
Fragments arranged by size:
2001 501 207
KasI G'GCGC_C
Cuts at: 0 738 2326 2361 2709
Size: 738 1588 35 348
Fragments arranged by size:
1588 738 348 35
KpnI G_GTAC'C
Cuts at: 0 73 1348 2709
Size: 73 1275 1361
Fragments arranged by size:
1361 1275 73
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1064 1307 2597 2709
Size: 366 698 243 1290 112
Fragments arranged by size:
1290 698 366 243 112
MscI TGG'CCA
Cuts at: 0 52 688 2709
Size: 52 636 2021
Fragments arranged by size:
2021 636 52
MslI CAynn'nnrTG
Cuts at: 0 601 1906 2709
Size: 601 1305 803
Fragments arranged by size:
1305 803 601
MspA1I CmG'CkG
Cuts at: 0 178 196 640 1204 1327 1520 1698 2163
Size: 178 18 444 564 123 193 178 465
Cuts at: 2163 2217 2256 2316 2340 2472 2523 2645 2709
Size: 54 39 60 24 132 51 122 64
Fragments arranged by size:
564 465 444 193 178 178 132 123
122 64 60 54 51 39 24 18
NarI GG'CG_CC
Cuts at: 0 739 2327 2362 2709
Size: 739 1588 35 347
Fragments arranged by size:
1588 739 347 35
NcoI C'CATG_G
Cuts at: 0 878 1635 2709
Size: 878 757 1074
Fragments arranged by size:
1074 878 757
NheI G'CTAG_C
Cuts at: 0 22 2709
Size: 22 2687
NspI r_CATG'y
Cuts at: 0 2406 2709
Size: 2406 303
PflMI CCAn_nnn'nTGG
Cuts at: 0 376 1958 2709
Size: 376 1582 751
Fragments arranged by size:
1582 751 376
PfoI T'CCnGG_A
Cuts at: 0 1552 2709
Size: 1552 1157
PpuMI rG'GwC_Cy
Cuts at: 0 1659 2709
Size: 1659 1050
PspOMI G'GGCC_C
Cuts at: 0 2115 2709
Size: 2115 594
PstI C_TGCA'G
Cuts at: 0 201 1898 2261 2709
Size: 201 1697 363 448
Fragments arranged by size:
1697 448 363 201
PvuII CAG'CTG
Cuts at: 0 178 196 640 1698 2256 2709
Size: 178 18 444 1058 558 453
Fragments arranged by size:
1058 558 453 444 178 18
SacI G_AGCT'C
Cuts at: 0 1448 2709
Size: 1448 1261
SacII CC_GC'GG
Cuts at: 0 1328 2317 2341 2473 2646 2709
Size: 1328 989 24 132 173 63
Fragments arranged by size:
1328 989 173 132 63 24
SexAI A'CCwGG_T
Cuts at: 0 1790 2709
Size: 1790 919
SfcI C'TryA_G
Cuts at: 0 197 663 1894 2257 2709
Size: 197 466 1231 363 452
Fragments arranged by size:
1231 466 452 363 197
SfoI GGC'GCC
Cuts at: 0 740 2328 2363 2709
Size: 740 1588 35 346
Fragments arranged by size:
1588 740 346 35
SmaI CCC'GGG
Cuts at: 0 1578 2114 2709
Size: 1578 536 595
Fragments arranged by size:
1578 595 536
SmlI C'TyrA_G
Cuts at: 0 1366 2668 2709
Size: 1366 1302 41
Fragments arranged by size:
1366 1302 41
SphI G_CATG'C
Cuts at: 0 2406 2709
Size: 2406 303
SrfI GCCC'GGGC
Cuts at: 0 2114 2709
Size: 2114 595
StuI AGG'CCT
Cuts at: 0 723 1279 1426 2695 2709
Size: 723 556 147 1269 14
Fragments arranged by size:
1269 723 556 147 14
StyI C'CwwG_G
Cuts at: 0 656 878 932 1160 1635 1742 1784 1808
Size: 656 222 54 228 475 107 42 24
Cuts at: 1808 2709
Size: 901
Fragments arranged by size:
901 656 475 228 222 107 54 42
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 987 2094 2245 2709
Size: 987 1107 151 464
Fragments arranged by size:
1107 987 464 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 850 882 1364 1396 2709
Size: 850 32 482 32 1313
Fragments arranged by size:
1313 850 482 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 972 2709
Size: 972 1737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 984 2709
Size: 600 384 1725
Fragments arranged by size:
1725 600 384
XmaI C'CCGG_G
Cuts at: 0 1576 2112 2709
Size: 1576 536 597
Fragments arranged by size:
1576 597 536
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssSI BstEII BstXI BstYI
BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NheI NspI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII
SexAI SfcI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AloI ApaLI AscI
AseI AsiSI AvrII BcgI BclI BfrBI BglII BsaAI
BsaWI BsaXI BsiWI BspEI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EcoRV FalI FseI FspI FspAI HindIII
HpaI MfeI MluI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SspI
SwaI TatI XbaI XhoI XmnI ZraI