Restriction map for
pF1KSDA1028
(Linear) (Six-base) MAPSORT of: KIAA1028.seq Check: 645 from: 1 to: 1506
KIAA1028.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1216 1506
Size: 1216 290
AccI GT'mk_AC
Cuts at: 0 104 1506
Size: 104 1402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1112 1316 1506
Size: 919 193 204 190
Fragments arranged by size:
919 204 193 190
AgeI A'CCGG_T
Cuts at: 0 1499 1506
Size: 1499 7
ApoI r'AATT_y
Cuts at: 0 1069 1506
Size: 1069 437
BamHI G'GATC_C
Cuts at: 0 570 954 993 1417 1506
Size: 570 384 39 424 89
Fragments arranged by size:
570 424 384 89 39
BanI G'GyrC_C
Cuts at: 0 88 1182 1252 1299 1506
Size: 88 1094 70 47 207
Fragments arranged by size:
1094 207 88 70 47
BanII G_rGCy'C
Cuts at: 0 603 1471 1506
Size: 603 868 35
Fragments arranged by size:
868 603 35
BbeI G_GCGC'C
Cuts at: 0 1186 1506
Size: 1186 320
BciVI GTATCCnnnnn_n'
Cuts at: 0 424 1506
Size: 424 1082
BclI T'GATC_A
Cuts at: 0 747 1506
Size: 747 759
BfrBI ATG'CAT
Cuts at: 0 148 1506
Size: 148 1358
BglII A'GATC_T
Cuts at: 0 373 1506
Size: 373 1133
Bme1580I G_kGCm'C
Cuts at: 0 1255 1506
Size: 1255 251
BmgBI CAC'GTC
Cuts at: 0 102 1506
Size: 102 1404
BmrI ACTGGGnnnn_n'
Cuts at: 0 807 1506
Size: 807 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1269 1365 1506
Size: 587 682 96 141
Fragments arranged by size:
682 587 141 96
BsaBI GATnn'nnATC
Cuts at: 0 569 1506
Size: 569 937
BsaHI Gr'CG_yC
Cuts at: 0 1183 1506
Size: 1183 323
BsaWI w'CCGG_w
Cuts at: 0 1255 1499 1506
Size: 1255 244 7
Fragments arranged by size:
1255 244 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 70 1137 1167 1506
Size: 40 30 1067 30 339
Fragments arranged by size:
1067 339 40 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 536 1506
Size: 536 970
BseYI C'CCAG_C
Cuts at: 0 1234 1506
Size: 1234 272
BsiHKAI G_wGCw'C
Cuts at: 0 1438 1471 1506
Size: 1438 33 35
Fragments arranged by size:
1438 35 33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1463 1506
Size: 1463 43
Bsp1286I G_dGCh'C
Cuts at: 0 603 1255 1438 1471 1506
Size: 603 652 183 33 35
Fragments arranged by size:
652 603 183 35 33
BspHI T'CATG_A
Cuts at: 0 744 1506
Size: 744 762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1216 1506
Size: 1216 290
BsrBI CCG'CTC
Cuts at: 0 36 42 1506
Size: 36 6 1464
Fragments arranged by size:
1464 36 6
BsrDI GCAATG_nn'
Cuts at: 0 238 1506
Size: 238 1268
BsrFI r'CCGG_y
Cuts at: 0 1039 1499 1506
Size: 1039 460 7
Fragments arranged by size:
1039 460 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 987 1506
Size: 987 519
BstYI r'GATC_y
Cuts at: 0 373 570 954 993 1417 1506
Size: 373 197 384 39 424 89
Fragments arranged by size:
424 384 373 197 89 39
BtgI C'CryG_G
Cuts at: 0 950 1318 1506
Size: 950 368 188
Fragments arranged by size:
950 368 188
EarI CTCTTCn'nnn_
Cuts at: 0 1455 1506
Size: 1455 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 132 1506
Size: 46 86 1374
Fragments arranged by size:
1374 86 46
EcoICRI GAG'CTC
Cuts at: 0 1469 1506
Size: 1469 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 919 1112 1269 1316 1365 1506
Size: 587 332 193 157 47 49 141
Fragments arranged by size:
587 332 193 157 141 49 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1294 1506
Size: 1294 212
EcoO109I rG'GnC_Cy
Cuts at: 0 487 1011 1327 1506
Size: 487 524 316 179
Fragments arranged by size:
524 487 316 179
EcoRI G'AATT_C
Cuts at: 0 1069 1506
Size: 1069 437
HaeII r_GCGC'y
Cuts at: 0 1186 1506
Size: 1186 320
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 939 971 1506
Size: 939 32 535
Fragments arranged by size:
939 535 32
HincII GTy'rAC
Cuts at: 0 683 1506
Size: 683 823
KasI G'GCGC_C
Cuts at: 0 1182 1506
Size: 1182 324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1506
Size: 982 524
MslI CAynn'nnrTG
Cuts at: 0 227 1506
Size: 227 1279
NaeI GCC'GGC
Cuts at: 0 1041 1506
Size: 1041 465
NarI GG'CG_CC
Cuts at: 0 1183 1506
Size: 1183 323
NcoI C'CATG_G
Cuts at: 0 950 1506
Size: 950 556
NgoMIV G'CCGG_C
Cuts at: 0 1039 1506
Size: 1039 467
NsiI A_TGCA'T
Cuts at: 0 150 1506
Size: 150 1356
PpuMI rG'GwC_Cy
Cuts at: 0 487 1011 1506
Size: 487 524 495
Fragments arranged by size:
524 495 487
PstI C_TGCA'G
Cuts at: 0 789 992 1506
Size: 789 203 514
Fragments arranged by size:
789 514 203
SacI G_AGCT'C
Cuts at: 0 1471 1506
Size: 1471 35
SexAI A'CCwGG_T
Cuts at: 0 1295 1506
Size: 1295 211
SfcI C'TryA_G
Cuts at: 0 785 988 1506
Size: 785 203 518
Fragments arranged by size:
785 518 203
SfoI GGC'GCC
Cuts at: 0 1184 1506
Size: 1184 322
StyI C'CwwG_G
Cuts at: 0 950 1506
Size: 950 556
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 426 1222 1496 1506
Size: 426 796 274 10
Fragments arranged by size:
796 426 274 10
TatI w'GTAC_w
Cuts at: 0 884 1506
Size: 884 622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 753 785 1137 1169 1212 1244 1506
Size: 753 32 352 32 43 32 262
Fragments arranged by size:
753 352 262 43 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 406 1438 1506
Size: 406 1032 68
Fragments arranged by size:
1032 406 68
XmnI GAAnn'nnTTC
Cuts at: 0 250 1506
Size: 250 1256
Enzymes that do cut:
AarI AccI AcuI AgeI ApoI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI
BpmI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstYI BtgI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII KasI MmeI MslI NaeI
NarI NcoI NgoMIV NsiI PpuMI PstI SacI SexAI
SfcI SfoI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BbvCI BcgI BglI BlpI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsgI BsiEI
BsiWI BsmI BspEI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MscI MspA1I
NdeI NheI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI ZraI