Restriction map for
pF1KSDA1024
(Linear) (Six-base) MAPSORT of: KIAA1024.seq Check: 3213 from: 1 to: 2748
KIAA1024.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2361 2748
Size: 2361 387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1795 2748
Size: 1795 953
AleI CACnn'nnGTG
Cuts at: 0 1030 1603 2748
Size: 1030 573 1145
Fragments arranged by size:
1145 1030 573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1813 2748
Size: 1813 935
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2359 2391 2748
Size: 2359 32 357
Fragments arranged by size:
2359 357 32
AlwNI CAG_nnn'CTG
Cuts at: 0 87 591 936 1058 2195 2748
Size: 87 504 345 122 1137 553
Fragments arranged by size:
1137 553 504 345 122 87
ApaI G_GGCC'C
Cuts at: 0 1025 2748
Size: 1025 1723
ApaLI G'TGCA_C
Cuts at: 0 1470 2748
Size: 1470 1278
ApoI r'AATT_y
Cuts at: 0 2647 2748
Size: 2647 101
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1600 1633 2748
Size: 1600 33 1115
Fragments arranged by size:
1600 1115 33
BamHI G'GATC_C
Cuts at: 0 1636 2748
Size: 1636 1112
BanI G'GyrC_C
Cuts at: 0 1229 1423 2748
Size: 1229 194 1325
Fragments arranged by size:
1325 1229 194
BanII G_rGCy'C
Cuts at: 0 407 1025 1304 1451 2748
Size: 407 618 279 147 1297
Fragments arranged by size:
1297 618 407 279 147
BbeI G_GCGC'C
Cuts at: 0 1233 2748
Size: 1233 1515
BbsI GAAGACnn'nnnn_
Cuts at: 0 1783 2603 2748
Size: 1783 820 145
Fragments arranged by size:
1783 820 145
BbvCI CC'TCA_GC
Cuts at: 0 617 2748
Size: 617 2131
BciVI GTATCCnnnnn_n'
Cuts at: 0 2608 2748
Size: 2608 140
BglII A'GATC_T
Cuts at: 0 38 380 1316 1932 2748
Size: 38 342 936 616 816
Fragments arranged by size:
936 816 616 342 38
BlpI GC'TnA_GC
Cuts at: 0 1399 2748
Size: 1399 1349
Bme1580I G_kGCm'C
Cuts at: 0 108 1025 1426 1474 2729 2748
Size: 108 917 401 48 1255 19
Fragments arranged by size:
1255 917 401 108 48 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 809 2748
Size: 809 1939
BmtI G_CTAG'C
Cuts at: 0 2060 2748
Size: 2060 688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1125 1357 1465 2594 2748
Size: 1125 232 108 1129 154
Fragments arranged by size:
1129 1125 232 154 108
Bpu10I CC'TnA_GC
Cuts at: 0 617 1679 1801 2748
Size: 617 1062 122 947
Fragments arranged by size:
1062 947 617 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2389 2748
Size: 2389 359
BsaI GGTCTCn'nnnn_
Cuts at: 0 709 1457 2748
Size: 709 748 1291
Fragments arranged by size:
1291 748 709
BsaAI yAC'GTr
Cuts at: 0 1440 2748
Size: 1440 1308
BsaHI Gr'CG_yC
Cuts at: 0 1230 2748
Size: 1230 1518
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 670 700 1990 2020 2055 2085 2359 2383
Size: 670 30 1290 30 35 30 274 24
Cuts at: 2383 2389 2413 2748
Size: 6 24 335
Fragments arranged by size:
1290 670 335 274 35 30 30 30
24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 748 1183 2155 2251 2254 2512 2748
Size: 748 435 972 96 3 258 236
Fragments arranged by size:
972 748 435 258 236 96 3
BseYI C'CCAG_C
Cuts at: 0 1215 2748
Size: 1215 1533
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1368 1945 2718 2748
Size: 1368 577 773 30
Fragments arranged by size:
1368 773 577 30
BsiHKAI G_wGCw'C
Cuts at: 0 149 854 1439 1474 1685 1802 2748
Size: 149 705 585 35 211 117 946
Fragments arranged by size:
946 705 585 211 149 117 35
BsmI GAATG_Cn'
Cuts at: 0 2517 2748
Size: 2517 231
Bsp1286I G_dGCh'C
Cuts at: 0 108 149 407 854 1025 1304 1426 1439
Size: 108 41 258 447 171 279 122 13
Cuts at: 1439 1451 1474 1685 1802 2729 2748
Size: 12 23 211 117 927 19
Fragments arranged by size:
927 447 279 258 211 171 122 117
108 41 23 19 13 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2361 2555 2748
Size: 2361 194 193
Fragments arranged by size:
2361 194 193
BsrDI GCAATG_nn'
Cuts at: 0 393 1274 2748
Size: 393 881 1474
Fragments arranged by size:
1474 881 393
BsrFI r'CCGG_y
Cuts at: 0 1739 2748
Size: 1739 1009
BsrGI T'GTAC_A
Cuts at: 0 2432 2748
Size: 2432 316
BstAPI GCAn_nnn'nTGC
Cuts at: 0 494 1382 2334 2512 2748
Size: 494 888 952 178 236
Fragments arranged by size:
952 888 494 236 178
BstXI CCAn_nnnn'nTGG
Cuts at: 0 289 1419 2223 2748
Size: 289 1130 804 525
Fragments arranged by size:
1130 804 525 289
BstYI r'GATC_y
Cuts at: 0 38 380 1316 1636 1932 2748
Size: 38 342 936 320 296 816
Fragments arranged by size:
936 816 342 320 296 38
BtgI C'CryG_G
Cuts at: 0 317 1598 2010 2748
Size: 317 1281 412 738
Fragments arranged by size:
1281 738 412 317
BtsI GCAGTG_nn'
Cuts at: 0 812 2748
Size: 812 1936
DraI TTT'AAA
Cuts at: 0 1365 2567 2748
Size: 1365 1202 181
Fragments arranged by size:
1365 1202 181
DraIII CAC_nnn'GTG
Cuts at: 0 2002 2748
Size: 2002 746
EaeI y'GGCC_r
Cuts at: 0 1090 2459 2748
Size: 1090 1369 289
Fragments arranged by size:
1369 1090 289
EarI CTCTTCn'nnn_
Cuts at: 0 31 154 1763 2113 2413 2748
Size: 31 123 1609 350 300 335
Fragments arranged by size:
1609 350 335 300 123 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1125 1357 1465 1795 2594 2748
Size: 1125 232 108 330 799 154
Fragments arranged by size:
1125 799 330 232 154 108
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2137 2233 2748
Size: 2137 96 515
Fragments arranged by size:
2137 515 96
EcoO109I rG'GnC_Cy
Cuts at: 0 84 588 638 1710 2129 2748
Size: 84 504 50 1072 419 619
Fragments arranged by size:
1072 619 504 419 84 50
EcoRI G'AATT_C
Cuts at: 0 2647 2748
Size: 2647 101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 909 2748
Size: 735 174 1839
Fragments arranged by size:
1839 735 174
HaeII r_GCGC'y
Cuts at: 0 1059 1233 2748
Size: 1059 174 1515
Fragments arranged by size:
1515 1059 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 600 632 670 702 1306 1338 1513 1545
Size: 600 32 38 32 604 32 175 32
Cuts at: 1545 1843 1875 1924 1945 1956 1960 1977 1992
Size: 298 32 49 21 11 4 17 15
Cuts at: 1992 2648 2664 2680 2696 2748
Size: 656 16 16 16 52
Fragments arranged by size:
656 604 600 298 175 52 49 38
32 32 32 32 32 21 17 16
16 16 15 11 4
HincII GTy'rAC
Cuts at: 0 1332 2748
Size: 1332 1416
HindIII A'AGCT_T
Cuts at: 0 987 2748
Size: 987 1761
KasI G'GCGC_C
Cuts at: 0 1229 2748
Size: 1229 1519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 2204 2748
Size: 350 1854 544
Fragments arranged by size:
1854 544 350
MscI TGG'CCA
Cuts at: 0 1092 2748
Size: 1092 1656
MslI CAynn'nnrTG
Cuts at: 0 1030 1603 2440 2748
Size: 1030 573 837 308
Fragments arranged by size:
1030 837 573 308
MspA1I CmG'CkG
Cuts at: 0 394 2748
Size: 394 2354
NarI GG'CG_CC
Cuts at: 0 1230 2748
Size: 1230 1518
NheI G'CTAG_C
Cuts at: 0 2056 2748
Size: 2056 692
NspI r_CATG'y
Cuts at: 0 2445 2702 2748
Size: 2445 257 46
Fragments arranged by size:
2445 257 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 1103 1869 2195 2748
Size: 1103 766 326 553
Fragments arranged by size:
1103 766 553 326
PfoI T'CCnGG_A
Cuts at: 0 2610 2748
Size: 2610 138
PmlI CAC'GTG
Cuts at: 0 1440 2748
Size: 1440 1308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2359 2391 2748
Size: 2359 32 357
Fragments arranged by size:
2359 357 32
PpuMI rG'GwC_Cy
Cuts at: 0 84 638 1710 2129 2748
Size: 84 554 1072 419 619
Fragments arranged by size:
1072 619 554 419 84
PspOMI G'GGCC_C
Cuts at: 0 1021 2748
Size: 1021 1727
PstI C_TGCA'G
Cuts at: 0 2552 2748
Size: 2552 196
PvuII CAG'CTG
Cuts at: 0 394 2748
Size: 394 2354
SanDI GG'GwC_CC
Cuts at: 0 1710 2748
Size: 1710 1038
SapI GCTCTTCn'nnn_
Cuts at: 0 154 1763 2413 2748
Size: 154 1609 650 335
Fragments arranged by size:
1609 650 335 154
ScaI AGT'ACT
Cuts at: 0 2587 2748
Size: 2587 161
SfcI C'TryA_G
Cuts at: 0 2548 2748
Size: 2548 200
SfoI GGC'GCC
Cuts at: 0 1231 2748
Size: 1231 1517
SmlI C'TyrA_G
Cuts at: 0 2404 2748
Size: 2404 344
SphI G_CATG'C
Cuts at: 0 2702 2748
Size: 2702 46
StuI AGG'CCT
Cuts at: 0 2092 2748
Size: 2092 656
StyI C'CwwG_G
Cuts at: 0 302 2660 2748
Size: 302 2358 88
Fragments arranged by size:
2358 302 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1441 1768 2467 2748
Size: 1441 327 699 281
Fragments arranged by size:
1441 699 327 281
TatI w'GTAC_w
Cuts at: 0 1490 2432 2585 2748
Size: 1490 942 153 163
Fragments arranged by size:
1490 942 163 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 1990 2022 2055 2087 2748
Size: 491 32 1467 32 33 32 661
Fragments arranged by size:
1467 661 491 33 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1032 1100 1999 2748
Size: 1032 68 899 749
Fragments arranged by size:
1032 899 749 68
XmnI GAAnn'nnTTC
Cuts at: 0 775 2748
Size: 775 1973
Enzymes that do cut:
AarI AcuI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI BtgI BtsI DraI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NarI NheI
NspI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SanDI SapI ScaI SfcI SfoI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AscI AseI AsiSI AvaI AvrII BcgI BclI
BfrBI BglI BmgBI BplI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NotI NruI NsiI
PacI PasI PciI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI ZraI