Restriction map for
pF1KA1009
(Linear) (Six-base) MAPSORT of: KIAA1009.seq Check: 1641 from: 1 to: 3981
KIAA1009.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 2954 3981
Size: 2954 1027
AccI GT'mk_AC
Cuts at: 0 1510 2222 3981
Size: 1510 712 1759
Fragments arranged by size:
1759 1510 712
Acc65I G'GTAC_C
Cuts at: 0 1227 3981
Size: 1227 2754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1085 1217 1352 2596 3088 3370 3383
Size: 955 130 132 135 1244 492 282 13
Cuts at: 3383 3632 3981
Size: 249 349
Fragments arranged by size:
1244 955 492 349 282 249 135 132
130 13
AflII C'TTAA_G
Cuts at: 0 40 63 3981
Size: 40 23 3918
Fragments arranged by size:
3918 40 23
AflIII A'CryG_T
Cuts at: 0 972 1086 3981
Size: 972 114 2895
Fragments arranged by size:
2895 972 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2822 3981
Size: 2822 1159
AlwNI CAG_nnn'CTG
Cuts at: 0 1543 2541 2629 3981
Size: 1543 998 88 1352
Fragments arranged by size:
1543 1352 998 88
ApaLI G'TGCA_C
Cuts at: 0 380 1250 3981
Size: 380 870 2731
Fragments arranged by size:
2731 870 380
ApoI r'AATT_y
Cuts at: 0 423 768 913 2118 2306 2531 2659 2995
Size: 423 345 145 1205 188 225 128 336
Cuts at: 2995 3492 3626 3981
Size: 497 134 355
Fragments arranged by size:
1205 497 423 355 345 336 225 188
145 134 128
AseI AT'TA_AT
Cuts at: 0 275 1166 3328 3981
Size: 275 891 2162 653
Fragments arranged by size:
2162 891 653 275
AvrII C'CTAG_G
Cuts at: 0 1423 3981
Size: 1423 2558
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1572 1605 3981
Size: 1572 33 2376
Fragments arranged by size:
2376 1572 33
BanI G'GyrC_C
Cuts at: 0 566 1227 3981
Size: 566 661 2754
Fragments arranged by size:
2754 661 566
BanII G_rGCy'C
Cuts at: 0 110 736 1309 2105 3981
Size: 110 626 573 796 1876
Fragments arranged by size:
1876 796 626 573 110
BbsI GAAGACnn'nnnn_
Cuts at: 0 695 2186 3981
Size: 695 1491 1795
Fragments arranged by size:
1795 1491 695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2050 2084 3981
Size: 2050 34 1897
Fragments arranged by size:
2050 1897 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1573 3981
Size: 1573 2408
BglI GCCn_nnn'nGGC
Cuts at: 0 3172 3981
Size: 3172 809
BglII A'GATC_T
Cuts at: 0 1180 1968 2127 3981
Size: 1180 788 159 1854
Fragments arranged by size:
1854 1180 788 159
Bme1580I G_kGCm'C
Cuts at: 0 384 452 1254 3981
Size: 384 68 802 2727
Fragments arranged by size:
2727 802 384 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 1319 3981
Size: 1319 2662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1889 2563 2719 3889 3981
Size: 1889 674 156 1170 92
Fragments arranged by size:
1889 1170 674 156 92
Bpu10I CC'TnA_GC
Cuts at: 0 557 1308 3981
Size: 557 751 2673
Fragments arranged by size:
2673 751 557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 737 944 1321 1416 2066 2464 3180
Size: 205 532 207 377 95 650 398 716
Cuts at: 3180 3521 3981
Size: 341 460
Fragments arranged by size:
716 650 532 460 398 377 341 207
205 95
BsaHI Gr'CG_yC
Cuts at: 0 2951 3981
Size: 2951 1030
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1990 2020 3923 3953 3981
Size: 1990 30 1903 30 28
Fragments arranged by size:
1990 1903 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 172 829 2011 3981
Size: 28 144 657 1182 1970
Fragments arranged by size:
1970 1182 657 144 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3904 3981
Size: 3904 77
BsiHKAI G_wGCw'C
Cuts at: 0 110 384 736 1254 1309 3981
Size: 110 274 352 518 55 2672
Fragments arranged by size:
2672 518 352 274 110 55
BsmI GAATG_Cn'
Cuts at: 0 2943 3972 3981
Size: 2943 1029 9
Fragments arranged by size:
2943 1029 9
Bsp1286I G_dGCh'C
Cuts at: 0 110 384 452 736 1254 1309 2105 3981
Size: 110 274 68 284 518 55 796 1876
Fragments arranged by size:
1876 796 518 284 274 110 68 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2610 3981
Size: 2610 1371
BsrDI GCAATG_nn'
Cuts at: 0 3425 3981
Size: 3425 556
BsrFI r'CCGG_y
Cuts at: 0 3923 3981
Size: 3923 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2521 3427 3981
Size: 2521 906 554
Fragments arranged by size:
2521 906 554
BstBI TT'CG_AA
Cuts at: 0 2228 3981
Size: 2228 1753
BstYI r'GATC_y
Cuts at: 0 1180 1968 2127 2538 3981
Size: 1180 788 159 411 1443
Fragments arranged by size:
1443 1180 788 411 159
Bsu36I CC'TnA_GG
Cuts at: 0 1575 3981
Size: 1575 2406
BtgI C'CryG_G
Cuts at: 0 2738 3981
Size: 2738 1243
BtsI GCAGTG_nn'
Cuts at: 0 54 3374 3981
Size: 54 3320 607
Fragments arranged by size:
3320 607 54
ClaI AT'CG_AT
Cuts at: 0 1402 3981
Size: 1402 2579
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3074 3109 3981
Size: 3074 35 872
Fragments arranged by size:
3074 872 35
DraI TTT'AAA
Cuts at: 0 261 1620 3981
Size: 261 1359 2361
Fragments arranged by size:
2361 1359 261
EaeI y'GGCC_r
Cuts at: 0 893 3981
Size: 893 3088
EarI CTCTTCn'nnn_
Cuts at: 0 5 398 758 1762 2572 3981
Size: 5 393 360 1004 810 1409
Fragments arranged by size:
1409 1004 810 393 360 5
EcoICRI GAG'CTC
Cuts at: 0 108 734 1307 3981
Size: 108 626 573 2674
Fragments arranged by size:
2674 626 573 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1085 1217 1352 1889 2563 2596 2719
Size: 955 130 132 135 537 674 33 123
Cuts at: 2719 3088 3370 3383 3632 3889 3981
Size: 369 282 13 249 257 92
Fragments arranged by size:
955 674 537 369 282 257 249 135
132 130 123 92 33 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2854 3981
Size: 2854 1127
EcoRI G'AATT_C
Cuts at: 0 423 768 2995 3981
Size: 423 345 2227 986
Fragments arranged by size:
2227 986 423 345
EcoRV GAT'ATC
Cuts at: 0 726 1930 2587 3981
Size: 726 1204 657 1394
Fragments arranged by size:
1394 1204 726 657
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1933 2416 2517 3562 3981
Size: 1933 483 101 1045 419
Fragments arranged by size:
1933 1045 483 419 101
FspI TGC'GCA
Cuts at: 0 1658 3981
Size: 1658 2323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 2571 2603 2971 3003 3145 3177
Size: 304 32 2235 32 368 32 142 32
Cuts at: 3177 3981
Size: 804
Fragments arranged by size:
2235 804 368 304 142 32 32 32
32
HincII GTy'rAC
Cuts at: 0 389 3981
Size: 389 3592
HindIII A'AGCT_T
Cuts at: 0 263 653 3981
Size: 263 390 3328
Fragments arranged by size:
3328 390 263
KpnI G_GTAC'C
Cuts at: 0 1231 3981
Size: 1231 2750
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1703 3981
Size: 1703 2278
MslI CAynn'nnrTG
Cuts at: 0 314 2936 3981
Size: 314 2622 1045
Fragments arranged by size:
2622 1045 314
MspA1I CmG'CkG
Cuts at: 0 1049 1855 2629 3981
Size: 1049 806 774 1352
Fragments arranged by size:
1352 1049 806 774
NcoI C'CATG_G
Cuts at: 0 2738 3981
Size: 2738 1243
NspI r_CATG'y
Cuts at: 0 480 976 1090 3751 3981
Size: 480 496 114 2661 230
Fragments arranged by size:
2661 496 480 230 114
PciI A'CATG_T
Cuts at: 0 972 1086 3981
Size: 972 114 2895
Fragments arranged by size:
2895 972 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 678 3981
Size: 678 3303
PsiI TTA'TAA
Cuts at: 0 1381 3550 3981
Size: 1381 2169 431
Fragments arranged by size:
2169 1381 431
PstI C_TGCA'G
Cuts at: 0 1470 3420 3584 3981
Size: 1470 1950 164 397
Fragments arranged by size:
1950 1470 397 164
PvuII CAG'CTG
Cuts at: 0 1049 1855 2629 3981
Size: 1049 806 774 1352
Fragments arranged by size:
1352 1049 806 774
SacI G_AGCT'C
Cuts at: 0 110 736 1309 3981
Size: 110 626 573 2672
Fragments arranged by size:
2672 626 573 110
ScaI AGT'ACT
Cuts at: 0 69 3981
Size: 69 3912
SfcI C'TryA_G
Cuts at: 0 1466 2990 3300 3416 3580 3631 3981
Size: 1466 1524 310 116 164 51 350
Fragments arranged by size:
1524 1466 350 310 164 116 51
SmlI C'TyrA_G
Cuts at: 0 40 63 184 752 959 1336 1395 2081
Size: 40 23 121 568 207 377 59 686
Cuts at: 2081 2443 3195 3536 3981
Size: 362 752 341 445
Fragments arranged by size:
752 686 568 445 377 362 341 207
121 59 40 23
SpeI A'CTAG_T
Cuts at: 0 93 3981
Size: 93 3888
SphI G_CATG'C
Cuts at: 0 480 3981
Size: 480 3501
SspI AAT'ATT
Cuts at: 0 951 1110 2612 3899 3981
Size: 951 159 1502 1287 82
Fragments arranged by size:
1502 1287 951 159 82
StyI C'CwwG_G
Cuts at: 0 562 1423 2738 3981
Size: 562 861 1315 1243
Fragments arranged by size:
1315 1243 861 562
TatI w'GTAC_w
Cuts at: 0 67 3981
Size: 67 3914
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3415 3447 3923 3955 3981
Size: 3415 32 476 32 26
Fragments arranged by size:
3415 476 32 32 26
XmnI GAAnn'nnTTC
Cuts at: 0 29 840 2570 2981 3626 3703 3981
Size: 29 811 1730 411 645 77 278
Fragments arranged by size:
1730 811 645 411 278 77 29
ZraI GAC'GTC
Cuts at: 0 2952 3981
Size: 2952 1029
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI AvrII BaeI BanI BanII BbsI
BcgI BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrFI BstAPI BstBI BstYI Bsu36I BtgI
BtsI ClaI CspCI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FalI FspI Hin4I HincII HindIII
KpnI MmeI MslI MspA1I NcoI NspI PciI PflMI
PsiI PstI PvuII SacI ScaI SfcI SmlI SpeI
SphI SspI StyI TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AhdI AleI AloI ApaI
AscI AsiSI AvaI BamHI BbeI BbvCI BclI BfrBI
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstEII BstXI BstZ17I BtgZI DraIII DrdI
EagI EciI EcoO109I FseI FspAI HaeII HpaI KasI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI