Restriction map for
pF1KA1008
(Linear) (Six-base) MAPSORT of: KIAA1008.seq Check: 1901 from: 1 to: 2784
KIAA1008.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1041 2784
Size: 1041 1743
AccI GT'mk_AC
Cuts at: 0 1343 2784
Size: 1343 1441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1703 1789 1835 2784
Size: 575 1128 86 46 949
Fragments arranged by size:
1128 949 575 86 46
AflII C'TTAA_G
Cuts at: 0 609 898 2784
Size: 609 289 1886
Fragments arranged by size:
1886 609 289
AleI CACnn'nnGTG
Cuts at: 0 211 2784
Size: 211 2573
AlwNI CAG_nnn'CTG
Cuts at: 0 176 2784
Size: 176 2608
ApoI r'AATT_y
Cuts at: 0 1055 1131 1164 1404 1763 1887 1903 1940
Size: 1055 76 33 240 359 124 16 37
Cuts at: 1940 1951 2009 2037 2361 2784
Size: 11 58 28 324 423
Fragments arranged by size:
1055 423 359 324 240 124 76 58
37 33 28 16 11
AseI AT'TA_AT
Cuts at: 0 1655 2497 2784
Size: 1655 842 287
Fragments arranged by size:
1655 842 287
AvaI C'yCGr_G
Cuts at: 0 32 100 2784
Size: 32 68 2684
Fragments arranged by size:
2684 68 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 1023 1319 2784
Size: 1023 296 1465
Fragments arranged by size:
1465 1023 296
BglII A'GATC_T
Cuts at: 0 1857 2784
Size: 1857 927
BlpI GC'TnA_GC
Cuts at: 0 1540 2208 2784
Size: 1540 668 576
Fragments arranged by size:
1540 668 576
BmgBI CAC'GTC
Cuts at: 0 1572 2784
Size: 1572 1212
BmrI ACTGGGnnnn_n'
Cuts at: 0 2412 2784
Size: 2412 372
BmtI G_CTAG'C
Cuts at: 0 671 2784
Size: 671 2113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1067 2601 2784
Size: 1067 1534 183
Fragments arranged by size:
1534 1067 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 255 261 1299 2784
Size: 163 92 6 1038 1485
Fragments arranged by size:
1485 1038 163 92 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 963 2784
Size: 963 1821
BsaBI GATnn'nnATC
Cuts at: 0 52 2195 2784
Size: 52 2143 589
Fragments arranged by size:
2143 589 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 2784
Size: 205 2579
BsiHKAI G_wGCw'C
Cuts at: 0 68 2784
Size: 68 2716
BsmI GAATG_Cn'
Cuts at: 0 899 2487 2717 2784
Size: 899 1588 230 67
Fragments arranged by size:
1588 899 230 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 71 2219 2784
Size: 71 2148 565
Fragments arranged by size:
2148 565 71
Bsp1286I G_dGCh'C
Cuts at: 0 68 2784
Size: 68 2716
BspHI T'CATG_A
Cuts at: 0 1944 2784
Size: 1944 840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 1041 2784
Size: 80 961 1743
Fragments arranged by size:
1743 961 80
BsrFI r'CCGG_y
Cuts at: 0 135 2784
Size: 135 2649
BssSI C'ACGA_G
Cuts at: 0 127 2784
Size: 127 2657
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 176 195 2784
Size: 72 104 19 2589
Fragments arranged by size:
2589 104 72 19
BstBI TT'CG_AA
Cuts at: 0 1974 2784
Size: 1974 810
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2070 2784
Size: 2070 714
BstYI r'GATC_y
Cuts at: 0 1857 2675 2784
Size: 1857 818 109
Fragments arranged by size:
1857 818 109
Bsu36I CC'TnA_GG
Cuts at: 0 1318 2784
Size: 1318 1466
BtgI C'CryG_G
Cuts at: 0 1740 1892 2784
Size: 1740 152 892
Fragments arranged by size:
1740 892 152
DraI TTT'AAA
Cuts at: 0 758 2784
Size: 758 2026
DraIII CAC_nnn'GTG
Cuts at: 0 1158 2784
Size: 1158 1626
EarI CTCTTCn'nnn_
Cuts at: 0 875 1479 2784
Size: 875 604 1305
Fragments arranged by size:
1305 875 604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 255 261 575 1299 1703 1789 1835
Size: 163 92 6 314 724 404 86 46
Cuts at: 1835 2784
Size: 949
Fragments arranged by size:
949 724 404 314 163 92 86 46
6
EcoO109I rG'GnC_Cy
Cuts at: 0 159 251 2784
Size: 159 92 2533
Fragments arranged by size:
2533 159 92
EcoRI G'AATT_C
Cuts at: 0 1055 2784
Size: 1055 1729
EcoRV GAT'ATC
Cuts at: 0 1141 2784
Size: 1141 1643
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 36 2784
Size: 36 2748
FseI GG_CCGG'CC
Cuts at: 0 139 2784
Size: 139 2645
FspI TGC'GCA
Cuts at: 0 108 2784
Size: 108 2676
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 1459 1491 2784
Size: 68 32 1359 32 1293
Fragments arranged by size:
1359 1293 68 32 32
HincII GTy'rAC
Cuts at: 0 2321 2784
Size: 2321 463
HindIII A'AGCT_T
Cuts at: 0 2335 2773 2784
Size: 2335 438 11
Fragments arranged by size:
2335 438 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1158 2784
Size: 1158 1626
MslI CAynn'nnrTG
Cuts at: 0 211 1353 1590 2201 2784
Size: 211 1142 237 611 583
Fragments arranged by size:
1142 611 583 237 211
MspA1I CmG'CkG
Cuts at: 0 414 2784
Size: 414 2370
NaeI GCC'GGC
Cuts at: 0 137 2784
Size: 137 2647
NcoI C'CATG_G
Cuts at: 0 1892 2784
Size: 1892 892
NdeI CA'TA_TG
Cuts at: 0 1677 2784
Size: 1677 1107
NgoMIV G'CCGG_C
Cuts at: 0 135 2784
Size: 135 2649
NheI G'CTAG_C
Cuts at: 0 667 2784
Size: 667 2117
NspI r_CATG'y
Cuts at: 0 987 1966 2784
Size: 987 979 818
Fragments arranged by size:
987 979 818
PflMI CCAn_nnn'nTGG
Cuts at: 0 979 2509 2784
Size: 979 1530 275
Fragments arranged by size:
1530 979 275
PpuMI rG'GwC_Cy
Cuts at: 0 159 251 2784
Size: 159 92 2533
Fragments arranged by size:
2533 159 92
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2687 2719 2784
Size: 2687 32 65
Fragments arranged by size:
2687 65 32
PstI C_TGCA'G
Cuts at: 0 1865 2784
Size: 1865 919
PvuII CAG'CTG
Cuts at: 0 414 2784
Size: 414 2370
SfcI C'TryA_G
Cuts at: 0 619 692 932 1853 1861 2784
Size: 619 73 240 921 8 923
Fragments arranged by size:
923 921 619 240 73 8
SmaI CCC'GGG
Cuts at: 0 34 102 2784
Size: 34 68 2682
Fragments arranged by size:
2682 68 34
SmlI C'TyrA_G
Cuts at: 0 4 609 898 2784
Size: 4 605 289 1886
Fragments arranged by size:
1886 605 289 4
SpeI A'CTAG_T
Cuts at: 0 1732 2784
Size: 1732 1052
SphI G_CATG'C
Cuts at: 0 987 2784
Size: 987 1797
SspI AAT'ATT
Cuts at: 0 590 1923 2135 2784
Size: 590 1333 212 649
Fragments arranged by size:
1333 649 590 212
StyI C'CwwG_G
Cuts at: 0 1463 1868 1892 2784
Size: 1463 405 24 892
Fragments arranged by size:
1463 892 405 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2256 2784
Size: 2256 528
TatI w'GTAC_w
Cuts at: 0 1357 2165 2305 2784
Size: 1357 808 140 479
Fragments arranged by size:
1357 808 479 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1142 1174 1481 1513 2784
Size: 1142 32 307 32 1271
Fragments arranged by size:
1271 1142 307 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1274 2784
Size: 1274 1510
XmaI C'CCGG_G
Cuts at: 0 32 100 2784
Size: 32 68 2684
Fragments arranged by size:
2684 68 32
XmnI GAAnn'nnTTC
Cuts at: 0 1701 2784
Size: 1701 1083
Enzymes that do cut:
AarI AccI AcuI AflII AleI AlwNI ApoI AseI
AvaI BbsI BglII BlpI BmgBI BmrI BmtI BplI
BpmI BsaI BsaBI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI DraI DraIII EarI Eco57MI EcoO109I EcoRI
EcoRV FalI FseI FspI Hin4I HincII HindIII MmeI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NspI
PflMI PpuMI PsrI PstI PvuII SfcI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI Bme1580I Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiWI BspEI BsrBI BsrDI BsrGI
BssHII BstEII BstZ17I BtgZI BtsI ClaI CspCI DrdI
EaeI EagI EciI EcoICRI EcoNI FspAI HaeII HpaI
KasI KpnI MfeI MluI MscI NarI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SrfI StuI SwaI Tth111I XbaI
XhoI ZraI