Restriction map for
pF1KA1002
(Linear) (Six-base) MAPSORT of: KIAA1002.seq Check: 3238 from: 1 to: 2520
KIAA1002.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1566 2520
Size: 1566 954
Acc65I G'GTAC_C
Cuts at: 0 1530 2520
Size: 1530 990
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 624 971 2131 2520
Size: 624 347 1160 389
Fragments arranged by size:
1160 624 389 347
AhdI GACnn_n'nnGTC
Cuts at: 0 619 2520
Size: 619 1901
AleI CACnn'nnGTG
Cuts at: 0 2386 2520
Size: 2386 134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 851 2520
Size: 851 1669
AlwNI CAG_nnn'CTG
Cuts at: 0 589 1239 1344 1934 2307 2520
Size: 589 650 105 590 373 213
Fragments arranged by size:
650 590 589 373 213 105
ApaLI G'TGCA_C
Cuts at: 0 2089 2520
Size: 2089 431
ApoI r'AATT_y
Cuts at: 0 58 133 340 2520
Size: 58 75 207 2180
Fragments arranged by size:
2180 207 75 58
AseI AT'TA_AT
Cuts at: 0 140 2520
Size: 140 2380
AvaI C'yCGr_G
Cuts at: 0 662 2520
Size: 662 1858
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1522 1555 2520
Size: 1522 33 965
Fragments arranged by size:
1522 965 33
BamHI G'GATC_C
Cuts at: 0 2495 2520
Size: 2495 25
BanI G'GyrC_C
Cuts at: 0 733 1530 2272 2520
Size: 733 797 742 248
Fragments arranged by size:
797 742 733 248
BanII G_rGCy'C
Cuts at: 0 1582 2520
Size: 1582 938
BbeI G_GCGC'C
Cuts at: 0 2276 2520
Size: 2276 244
BbvCI CC'TCA_GC
Cuts at: 0 854 904 1007 1244 1924 2520
Size: 854 50 103 237 680 596
Fragments arranged by size:
854 680 596 237 103 50
BclI T'GATC_A
Cuts at: 0 243 2520
Size: 243 2277
BglII A'GATC_T
Cuts at: 0 2208 2520
Size: 2208 312
BlpI GC'TnA_GC
Cuts at: 0 1348 1562 2520
Size: 1348 214 958
Fragments arranged by size:
1348 958 214
Bme1580I G_kGCm'C
Cuts at: 0 738 2093 2520
Size: 738 1355 427
Fragments arranged by size:
1355 738 427
BmrI ACTGGGnnnn_n'
Cuts at: 0 260 762 1887 2520
Size: 260 502 1125 633
Fragments arranged by size:
1125 633 502 260
BmtI G_CTAG'C
Cuts at: 0 635 2520
Size: 635 1885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 696 1838 1883 2218 2333 2520
Size: 580 116 1142 45 335 115 187
Fragments arranged by size:
1142 580 335 187 116 115 45
Bpu10I CC'TnA_GC
Cuts at: 0 854 904 1007 1244 1924 2300 2520
Size: 854 50 103 237 680 376 220
Fragments arranged by size:
854 680 376 237 220 103 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 501 2275 2520
Size: 346 155 1774 245
Fragments arranged by size:
1774 346 245 155
BsaI GGTCTCn'nnnn_
Cuts at: 0 1814 2520
Size: 1814 706
BsaHI Gr'CG_yC
Cuts at: 0 2273 2520
Size: 2273 247
BsaWI w'CCGG_w
Cuts at: 0 191 602 2520
Size: 191 411 1918
Fragments arranged by size:
1918 411 191
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1764 1794 1881 1911 2520
Size: 1764 30 87 30 609
Fragments arranged by size:
1764 609 87 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1374 2520
Size: 1374 1146
BseYI C'CCAG_C
Cuts at: 0 800 998 1253 1518 1739 2401 2520
Size: 800 198 255 265 221 662 119
Fragments arranged by size:
800 662 265 255 221 198 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1920 2520
Size: 1920 600
BsiHKAI G_wGCw'C
Cuts at: 0 2093 2520
Size: 2093 427
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2440 2520
Size: 2440 80
Bsp1286I G_dGCh'C
Cuts at: 0 738 1582 2093 2520
Size: 738 844 511 427
Fragments arranged by size:
844 738 511 427
BspEI T'CCGG_A
Cuts at: 0 602 2520
Size: 602 1918
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1566 2520
Size: 1566 954
BsrGI T'GTAC_A
Cuts at: 0 1826 2378 2520
Size: 1826 552 142
Fragments arranged by size:
1826 552 142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 733 1101 1479 1934 2520
Size: 733 368 378 455 586
Fragments arranged by size:
733 586 455 378 368
BstBI TT'CG_AA
Cuts at: 0 2466 2520
Size: 2466 54
BstEII G'GTnAC_C
Cuts at: 0 756 2520
Size: 756 1764
BstXI CCAn_nnnn'nTGG
Cuts at: 0 572 1261 2163 2409 2520
Size: 572 689 902 246 111
Fragments arranged by size:
902 689 572 246 111
BstYI r'GATC_y
Cuts at: 0 707 1136 2208 2279 2495 2520
Size: 707 429 1072 71 216 25
Fragments arranged by size:
1072 707 429 216 71 25
Bsu36I CC'TnA_GG
Cuts at: 0 2216 2491 2520
Size: 2216 275 29
Fragments arranged by size:
2216 275 29
BtgI C'CryG_G
Cuts at: 0 1034 1760 2067 2520
Size: 1034 726 307 453
Fragments arranged by size:
1034 726 453 307
BtsI GCAGTG_nn'
Cuts at: 0 702 1408 1935 2520
Size: 702 706 527 585
Fragments arranged by size:
706 702 585 527
DraIII CAC_nnn'GTG
Cuts at: 0 733 1209 1999 2520
Size: 733 476 790 521
Fragments arranged by size:
790 733 521 476
EaeI y'GGCC_r
Cuts at: 0 405 2070 2352 2471 2520
Size: 405 1665 282 119 49
Fragments arranged by size:
1665 405 282 119 49
EarI CTCTTCn'nnn_
Cuts at: 0 2254 2520
Size: 2254 266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2254 2520
Size: 2254 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 624 696 971 1838 1883 2131 2218
Size: 580 44 72 275 867 45 248 87
Cuts at: 2218 2333 2520
Size: 115 187
Fragments arranged by size:
867 580 275 248 187 115 87 72
45 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1586 2520
Size: 1586 934
EcoO109I rG'GnC_Cy
Cuts at: 0 1341 2520
Size: 1341 1179
EcoRV GAT'ATC
Cuts at: 0 418 2520
Size: 418 2102
HaeII r_GCGC'y
Cuts at: 0 2010 2276 2520
Size: 2010 266 244
Fragments arranged by size:
2010 266 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 171 203 605 637 918 950 2126 2158
Size: 171 32 402 32 281 32 1176 32
Cuts at: 2158 2520
Size: 362
Fragments arranged by size:
1176 402 362 281 171 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2078 2520
Size: 2078 442
KasI G'GCGC_C
Cuts at: 0 2272 2520
Size: 2272 248
KpnI G_GTAC'C
Cuts at: 0 1534 2520
Size: 1534 986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1202 2520
Size: 1082 120 1318
Fragments arranged by size:
1318 1082 120
MscI TGG'CCA
Cuts at: 0 407 2072 2473 2520
Size: 407 1665 401 47
Fragments arranged by size:
1665 407 401 47
MslI CAynn'nnrTG
Cuts at: 0 1861 2386 2520
Size: 1861 525 134
Fragments arranged by size:
1861 525 134
MspA1I CmG'CkG
Cuts at: 0 958 1239 1525 1719 1803 2520
Size: 958 281 286 194 84 717
Fragments arranged by size:
958 717 286 281 194 84
NarI GG'CG_CC
Cuts at: 0 2273 2520
Size: 2273 247
NcoI C'CATG_G
Cuts at: 0 1760 2067 2520
Size: 1760 307 453
Fragments arranged by size:
1760 453 307
NheI G'CTAG_C
Cuts at: 0 631 2520
Size: 631 1889
NspI r_CATG'y
Cuts at: 0 612 1122 2520
Size: 612 510 1398
Fragments arranged by size:
1398 612 510
PasI CC'CwG_GG
Cuts at: 0 1110 2520
Size: 1110 1410
PfoI T'CCnGG_A
Cuts at: 0 2498 2520
Size: 2498 22
PstI C_TGCA'G
Cuts at: 0 972 1651 2520
Size: 972 679 869
Fragments arranged by size:
972 869 679
PvuII CAG'CTG
Cuts at: 0 958 1239 1719 2520
Size: 958 281 480 801
Fragments arranged by size:
958 801 480 281
ScaI AGT'ACT
Cuts at: 0 1675 2520
Size: 1675 845
SexAI A'CCwGG_T
Cuts at: 0 752 1698 2520
Size: 752 946 822
Fragments arranged by size:
946 822 752
SfcI C'TryA_G
Cuts at: 0 968 1215 1542 1586 1647 1788 2520
Size: 968 247 327 44 61 141 732
Fragments arranged by size:
968 732 327 247 141 61 44
SfoI GGC'GCC
Cuts at: 0 2274 2520
Size: 2274 246
SmlI C'TyrA_G
Cuts at: 0 361 516 2290 2520
Size: 361 155 1774 230
Fragments arranged by size:
1774 361 230 155
SpeI A'CTAG_T
Cuts at: 0 277 2520
Size: 277 2243
SphI G_CATG'C
Cuts at: 0 612 2520
Size: 612 1908
StuI AGG'CCT
Cuts at: 0 1388 1514 2520
Size: 1388 126 1006
Fragments arranged by size:
1388 1006 126
StyI C'CwwG_G
Cuts at: 0 88 571 939 1305 1760 2067 2520
Size: 88 483 368 366 455 307 453
Fragments arranged by size:
483 455 453 368 366 307 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 401 861 2520
Size: 401 460 1659
Fragments arranged by size:
1659 460 401
TatI w'GTAC_w
Cuts at: 0 793 1400 1538 1673 1826 2036 2378 2520
Size: 793 607 138 135 153 210 342 142
Fragments arranged by size:
793 607 342 210 153 142 138 135
Tth111I GACn'n_nGTC
Cuts at: 0 1946 2520
Size: 1946 574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 720 1312 2101 2520
Size: 720 592 789 419
Fragments arranged by size:
789 720 592 419
Enzymes that do cut:
AarI Acc65I AcuI AhdI AleI AlfI AlwNI ApaLI
ApoI AseI AvaI BaeI BamHI BanI BanII BbeI
BbvCI BclI BglII BlpI Bme1580I BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrGI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NheI NspI PasI PfoI PstI PvuII
ScaI SexAI SfcI SfoI SmlI SpeI SphI StuI
StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AloI
ApaI AscI AsiSI AvrII BbsI BcgI BciVI BfrBI
BglI BmgBI BplI BsaAI BsaBI BsiEI BsiWI BsmI
BspHI BsrBI BsrDI BsrFI BssHII BssSI BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EcoICRI EcoRI FalI
FseI FspI FspAI HindIII HpaI MfeI MluI NaeI
NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SfiI SgfI SgrAI SmaI SnaBI SrfI SspI
SwaI TstI XbaI XhoI XmaI XmnI ZraI