Restriction map for
pF1KSDA1001
(Linear) (Six-base) MAPSORT of: KIAA1001.seq Check: 2320 from: 1 to: 1575
KIAA1001.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 256 1575
Size: 256 1319
AatII G_ACGT'C
Cuts at: 0 1172 1466 1575
Size: 1172 294 109
Fragments arranged by size:
1172 294 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1505 1575
Size: 1505 70
AgeI A'CCGG_T
Cuts at: 0 1300 1575
Size: 1300 275
AleI CACnn'nnGTG
Cuts at: 0 328 592 1575
Size: 328 264 983
Fragments arranged by size:
983 328 264
AlwNI CAG_nnn'CTG
Cuts at: 0 1393 1575
Size: 1393 182
AvaI C'yCGr_G
Cuts at: 0 260 1438 1575
Size: 260 1178 137
Fragments arranged by size:
1178 260 137
BanI G'GyrC_C
Cuts at: 0 1019 1575
Size: 1019 556
BanII G_rGCy'C
Cuts at: 0 818 921 1257 1575
Size: 818 103 336 318
Fragments arranged by size:
818 336 318 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 1382 1575
Size: 1382 193
BglI GCCn_nnn'nGGC
Cuts at: 0 287 1575
Size: 287 1288
Bme1580I G_kGCm'C
Cuts at: 0 758 1022 1208 1400 1575
Size: 758 264 186 192 175
Fragments arranged by size:
758 264 192 186 175
BmgBI CAC'GTC
Cuts at: 0 594 1575
Size: 594 981
BmrI ACTGGGnnnn_n'
Cuts at: 0 171 404 678 1575
Size: 171 233 274 897
Fragments arranged by size:
897 274 233 171
BmtI G_CTAG'C
Cuts at: 0 937 1575
Size: 937 638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1262 1294 1479 1575
Size: 494 768 32 185 96
Fragments arranged by size:
768 494 185 96 32
Bpu10I CC'TnA_GC
Cuts at: 0 1337 1575
Size: 1337 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 1127 1575
Size: 666 461 448
Fragments arranged by size:
666 461 448
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 1575
Size: 346 1229
BsaAI yAC'GTr
Cuts at: 0 753 1575
Size: 753 822
BsaHI Gr'CG_yC
Cuts at: 0 1169 1463 1575
Size: 1169 294 112
Fragments arranged by size:
1169 294 112
BsaWI w'CCGG_w
Cuts at: 0 1300 1575
Size: 1300 275
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 492 522 1477 1507 1575
Size: 492 30 955 30 68
Fragments arranged by size:
955 492 68 30 30
BseYI C'CCAG_C
Cuts at: 0 1029 1575
Size: 1029 546
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1575
Size: 830 745
BsiEI CG_ry'CG
Cuts at: 0 282 1575
Size: 282 1293
BsiHKAI G_wGCw'C
Cuts at: 0 1184 1330 1575
Size: 1184 146 245
Fragments arranged by size:
1184 245 146
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1176 1575
Size: 1176 399
Bsp1286I G_dGCh'C
Cuts at: 0 758 818 921 1022 1184 1208 1257 1330
Size: 758 60 103 101 162 24 49 73
Cuts at: 1330 1400 1575
Size: 70 175
Fragments arranged by size:
758 175 162 103 101 73 70 60
49 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 256 1575
Size: 256 1319
BsrBI CCG'CTC
Cuts at: 0 345 1575
Size: 345 1230
BsrDI GCAATG_nn'
Cuts at: 0 303 1475 1575
Size: 303 1172 100
Fragments arranged by size:
1172 303 100
BsrFI r'CCGG_y
Cuts at: 0 277 281 636 1190 1300 1575
Size: 277 4 355 554 110 275
Fragments arranged by size:
554 355 277 275 110 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1212 1575
Size: 1212 363
BstEII G'GTnAC_C
Cuts at: 0 145 1575
Size: 145 1430
BtgI C'CryG_G
Cuts at: 0 426 450 787 913 1575
Size: 426 24 337 126 662
Fragments arranged by size:
662 426 337 126 24
BtsI GCAGTG_nn'
Cuts at: 0 179 948 1076 1575
Size: 179 769 128 499
Fragments arranged by size:
769 499 179 128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 432 467 511 546 1575
Size: 432 35 44 35 1029
Fragments arranged by size:
1029 432 44 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 550 870 1212 1575
Size: 550 320 342 363
Fragments arranged by size:
550 363 342 320
EaeI y'GGCC_r
Cuts at: 0 125 279 1188 1575
Size: 125 154 909 387
Fragments arranged by size:
909 387 154 125
EagI C'GGCC_G
Cuts at: 0 279 1575
Size: 279 1296
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1258 1575
Size: 1258 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1262 1294 1479 1505 1575
Size: 494 768 32 185 26 70
Fragments arranged by size:
768 494 185 70 32 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1126 1575
Size: 1126 449
EcoO109I rG'GnC_Cy
Cuts at: 0 716 949 1026 1333 1515 1575
Size: 716 233 77 307 182 60
Fragments arranged by size:
716 307 233 182 77 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 126 1575
Size: 126 1449
HaeII r_GCGC'y
Cuts at: 0 787 1155 1575
Size: 787 368 420
Fragments arranged by size:
787 420 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 544 576 816 848 1477 1509 1575
Size: 544 32 240 32 629 32 66
Fragments arranged by size:
629 544 240 66 32 32 32
HincII GTy'rAC
Cuts at: 0 861 1575
Size: 861 714
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1575
Size: 1132 443
MslI CAynn'nnrTG
Cuts at: 0 328 592 751 1575
Size: 328 264 159 824
Fragments arranged by size:
824 328 264 159
MspA1I CmG'CkG
Cuts at: 0 238 710 1232 1240 1562 1575
Size: 238 472 522 8 322 13
Fragments arranged by size:
522 472 322 238 13 8
NaeI GCC'GGC
Cuts at: 0 283 1575
Size: 283 1292
NgoMIV G'CCGG_C
Cuts at: 0 281 1575
Size: 281 1294
NheI G'CTAG_C
Cuts at: 0 933 1575
Size: 933 642
NspI r_CATG'y
Cuts at: 0 750 1575
Size: 750 825
PflMI CCAn_nnn'nTGG
Cuts at: 0 492 1575
Size: 492 1083
PmlI CAC'GTG
Cuts at: 0 753 1575
Size: 753 822
PpuMI rG'GwC_Cy
Cuts at: 0 949 1026 1515 1575
Size: 949 77 489 60
Fragments arranged by size:
949 489 77 60
PstI C_TGCA'G
Cuts at: 0 377 1262 1346 1575
Size: 377 885 84 229
Fragments arranged by size:
885 377 229 84
PvuII CAG'CTG
Cuts at: 0 238 1240 1575
Size: 238 1002 335
Fragments arranged by size:
1002 335 238
SanDI GG'GwC_CC
Cuts at: 0 949 1026 1575
Size: 949 77 549
Fragments arranged by size:
949 549 77
SfcI C'TryA_G
Cuts at: 0 373 1258 1342 1575
Size: 373 885 84 233
Fragments arranged by size:
885 373 233 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 287 1575
Size: 287 1288
SmaI CCC'GGG
Cuts at: 0 262 1575
Size: 262 1313
SmlI C'TyrA_G
Cuts at: 0 645 1142 1575
Size: 645 497 433
Fragments arranged by size:
645 497 433
StuI AGG'CCT
Cuts at: 0 338 1288 1575
Size: 338 950 287
Fragments arranged by size:
950 338 287
StyI C'CwwG_G
Cuts at: 0 359 1575
Size: 359 1216
TatI w'GTAC_w
Cuts at: 0 499 1575
Size: 499 1076
XmaI C'CCGG_G
Cuts at: 0 260 1575
Size: 260 1315
ZraI GAC'GTC
Cuts at: 0 1170 1464 1575
Size: 1170 294 111
Fragments arranged by size:
1170 294 111
Enzymes that do cut:
AarI AatII AcuI AgeI AleI AlwNI AvaI BanI
BanII BbsI BglI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstEII BtgI BtsI CspCI DraIII EaeI
EagI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII MmeI MslI MspA1I NaeI NgoMIV NheI NspI
PflMI PmlI PpuMI PstI PvuII SanDI SfcI SfiI
SmaI SmlI StuI StyI TatI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BplI BsaBI BseRI BsiWI BsmI BspEI
BspHI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI DraI DrdI EarI EcoICRI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI