Restriction map for
pF1KSDA0997
(Linear) (Six-base) MAPSORT of: KIAA0997.seq Check: 5503 from: 1 to: 1932
KIAA0997.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1115 1372 1932
Size: 16 1099 257 560
Fragments arranged by size:
1099 560 257 16
AflII C'TTAA_G
Cuts at: 0 1776 1932
Size: 1776 156
AflIII A'CryG_T
Cuts at: 0 618 1828 1932
Size: 618 1210 104
Fragments arranged by size:
1210 618 104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 194 1932
Size: 194 1738
ApoI r'AATT_y
Cuts at: 0 799 1932
Size: 799 1133
BanII G_rGCy'C
Cuts at: 0 1418 1932
Size: 1418 514
BbsI GAAGACnn'nnnn_
Cuts at: 0 347 1932
Size: 347 1585
BciVI GTATCCnnnnn_n'
Cuts at: 0 1570 1932
Size: 1570 362
BfrBI ATG'CAT
Cuts at: 0 1261 1932
Size: 1261 671
BglII A'GATC_T
Cuts at: 0 742 1422 1932
Size: 742 680 510
Fragments arranged by size:
742 680 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1932
Size: 258 1674
Bpu10I CC'TnA_GC
Cuts at: 0 557 1932
Size: 557 1375
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1288 1318 1932
Size: 1288 30 614
Fragments arranged by size:
1288 614 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 869 1309 1468 1932
Size: 869 440 159 464
Fragments arranged by size:
869 464 440 159
BseYI C'CCAG_C
Cuts at: 0 10 1932
Size: 10 1922
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 233 500 818 1852 1932
Size: 164 69 267 318 1034 80
Fragments arranged by size:
1034 318 267 164 80 69
Bsp1286I G_dGCh'C
Cuts at: 0 1418 1932
Size: 1418 514
BspHI T'CATG_A
Cuts at: 0 158 1932
Size: 158 1774
BsrDI GCAATG_nn'
Cuts at: 0 305 465 1932
Size: 305 160 1467
Fragments arranged by size:
1467 305 160
BstYI r'GATC_y
Cuts at: 0 742 1422 1932
Size: 742 680 510
Fragments arranged by size:
742 680 510
DraI TTT'AAA
Cuts at: 0 291 1581 1932
Size: 291 1290 351
Fragments arranged by size:
1290 351 291
EaeI y'GGCC_r
Cuts at: 0 1617 1892 1932
Size: 1617 275 40
Fragments arranged by size:
1617 275 40
EarI CTCTTCn'nnn_
Cuts at: 0 387 524 935 1091 1466 1932
Size: 387 137 411 156 375 466
Fragments arranged by size:
466 411 387 375 156 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 654 1932
Size: 654 1278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 258 1115 1372 1932
Size: 16 242 857 257 560
Fragments arranged by size:
857 560 257 242 16
EcoO109I rG'GnC_Cy
Cuts at: 0 1217 1299 1909 1932
Size: 1217 82 610 23
Fragments arranged by size:
1217 610 82 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 1288 1291 1320 1323 1474 1506
Size: 517 32 739 3 29 3 151 32
Cuts at: 1506 1932
Size: 426
Fragments arranged by size:
739 517 426 151 32 32 29 3
3
MfeI C'AATT_G
Cuts at: 0 86 1932
Size: 86 1846
MscI TGG'CCA
Cuts at: 0 1619 1894 1932
Size: 1619 275 38
Fragments arranged by size:
1619 275 38
MspA1I CmG'CkG
Cuts at: 0 1767 1932
Size: 1767 165
NdeI CA'TA_TG
Cuts at: 0 1655 1932
Size: 1655 277
NsiI A_TGCA'T
Cuts at: 0 1263 1932
Size: 1263 669
NspI r_CATG'y
Cuts at: 0 703 1349 1399 1832 1932
Size: 703 646 50 433 100
Fragments arranged by size:
703 646 433 100 50
PciI A'CATG_T
Cuts at: 0 1828 1932
Size: 1828 104
PflMI CCAn_nnn'nTGG
Cuts at: 0 473 1932
Size: 473 1459
PfoI T'CCnGG_A
Cuts at: 0 347 1932
Size: 347 1585
PpuMI rG'GwC_Cy
Cuts at: 0 1299 1932
Size: 1299 633
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 570 602 1932
Size: 570 32 1330
Fragments arranged by size:
1330 570 32
SapI GCTCTTCn'nnn_
Cuts at: 0 1091 1932
Size: 1091 841
ScaI AGT'ACT
Cuts at: 0 177 1932
Size: 177 1755
SfcI C'TryA_G
Cuts at: 0 132 310 1004 1932
Size: 132 178 694 928
Fragments arranged by size:
928 694 178 132
SmlI C'TyrA_G
Cuts at: 0 1776 1932
Size: 1776 156
TatI w'GTAC_w
Cuts at: 0 175 712 1239 1932
Size: 175 537 527 693
Fragments arranged by size:
693 537 527 175
XmnI GAAnn'nnTTC
Cuts at: 0 998 1932
Size: 998 934
Enzymes that do cut:
AcuI AflII AflIII AlfI ApoI BanII BbsI BciVI
BfrBI BglII BpmI Bpu10I BsaXI BseRI BseYI BsgI
Bsp1286I BspHI BsrDI BstYI DraI EaeI EarI EciI
Eco57MI EcoO109I Hin4I MfeI MscI MspA1I NdeI NsiI
NspI PciI PflMI PfoI PpuMI PsrI SapI ScaI
SfcI SmlI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MmeI
MslI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI