Restriction map for
pF1KA0985
(Linear) (Six-base) MAPSORT of: KIAA0985.seq Check: 7927 from: 1 to: 2082
KIAA0985.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1611 2082
Size: 1611 471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1531 2082
Size: 1276 255 551
Fragments arranged by size:
1276 551 255
AfeI AGC'GCT
Cuts at: 0 1990 2041 2082
Size: 1990 51 41
Fragments arranged by size:
1990 51 41
AflIII A'CryG_T
Cuts at: 0 2069 2082
Size: 2069 13
AleI CACnn'nnGTG
Cuts at: 0 110 2082
Size: 110 1972
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1748 2082
Size: 1748 334
AlwNI CAG_nnn'CTG
Cuts at: 0 652 664 2082
Size: 652 12 1418
Fragments arranged by size:
1418 652 12
ApaI G_GGCC'C
Cuts at: 0 634 2082
Size: 634 1448
ApaLI G'TGCA_C
Cuts at: 0 1230 1723 2082
Size: 1230 493 359
Fragments arranged by size:
1230 493 359
ApoI r'AATT_y
Cuts at: 0 1189 1459 1474 1530 1846 2082
Size: 1189 270 15 56 316 236
Fragments arranged by size:
1189 316 270 236 56 15
AvaI C'yCGr_G
Cuts at: 0 188 593 605 633 670 983 1592 2082
Size: 188 405 12 28 37 313 609 490
Fragments arranged by size:
609 490 405 313 188 37 28 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1995 2028 2082
Size: 1995 33 54
Fragments arranged by size:
1995 54 33
BanI G'GyrC_C
Cuts at: 0 1180 2045 2082
Size: 1180 865 37
Fragments arranged by size:
1180 865 37
BanII G_rGCy'C
Cuts at: 0 328 581 600 634 727 790 856 2082
Size: 328 253 19 34 93 63 66 1226
Fragments arranged by size:
1226 328 253 93 66 63 34 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 526 649 1432 2082
Size: 526 123 783 650
Fragments arranged by size:
783 650 526 123
BbvCI CC'TCA_GC
Cuts at: 0 866 2082
Size: 866 1216
BglI GCCn_nnn'nGGC
Cuts at: 0 126 712 2082
Size: 126 586 1370
Fragments arranged by size:
1370 586 126
Bme1580I G_kGCm'C
Cuts at: 0 634 1185 1234 1727 2082
Size: 634 551 49 493 355
Fragments arranged by size:
634 551 493 355 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 41 2082
Size: 41 2041
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 293 479 584 762 791 810 1567
Size: 70 223 186 105 178 29 19 757
Cuts at: 1567 2082
Size: 515
Fragments arranged by size:
757 515 223 186 178 105 70 29
19
Bpu10I CC'TnA_GC
Cuts at: 0 575 866 2082
Size: 575 291 1216
Fragments arranged by size:
1216 575 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1486 2082
Size: 1486 596
BsaI GGTCTCn'nnnn_
Cuts at: 0 379 1375 1480 1671 2082
Size: 379 996 105 191 411
Fragments arranged by size:
996 411 379 191 105
BsaAI yAC'GTr
Cuts at: 0 2070 2082
Size: 2070 12
BsaHI Gr'CG_yC
Cuts at: 0 231 2082
Size: 231 1851
BsaWI w'CCGG_w
Cuts at: 0 1057 1785 2082
Size: 1057 728 297
Fragments arranged by size:
1057 728 297
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 266 296 479 509 1204 1234 2082
Size: 266 30 183 30 695 30 848
Fragments arranged by size:
848 695 266 183 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 1003 1141 1144 1660 1870 2082
Size: 172 831 138 3 516 210 212
Fragments arranged by size:
831 516 212 210 172 138 3
BseYI C'CCAG_C
Cuts at: 0 312 321 530 657 749 789 855 1017
Size: 312 9 209 127 92 40 66 162
Cuts at: 1017 1091 1573 1965 2082
Size: 74 482 392 117
Fragments arranged by size:
482 392 312 209 162 127 117 92
74 66 40 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 868 1285 2082
Size: 351 517 417 797
Fragments arranged by size:
797 517 417 351
BsiHKAI G_wGCw'C
Cuts at: 0 727 1234 1727 2082
Size: 727 507 493 355
Fragments arranged by size:
727 507 493 355
BsmI GAATG_Cn'
Cuts at: 0 402 2082
Size: 402 1680
BsmBI CGTCTCn'nnnn_
Cuts at: 0 795 2082
Size: 795 1287
Bsp1286I G_dGCh'C
Cuts at: 0 328 581 600 634 727 790 856 1185
Size: 328 253 19 34 93 63 66 329
Cuts at: 1185 1234 1727 2082
Size: 49 493 355
Fragments arranged by size:
493 355 329 328 253 93 66 63
49 34 19
BspEI T'CCGG_A
Cuts at: 0 1057 2082
Size: 1057 1025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 784 840 1611 2082
Size: 784 56 771 471
Fragments arranged by size:
784 771 471 56
BsrFI r'CCGG_y
Cuts at: 0 53 2082
Size: 53 2029
BsrGI T'GTAC_A
Cuts at: 0 1676 2082
Size: 1676 406
BssSI C'ACGA_G
Cuts at: 0 1387 2082
Size: 1387 695
BstAPI GCAn_nnn'nTGC
Cuts at: 0 374 425 1308 2082
Size: 374 51 883 774
Fragments arranged by size:
883 774 374 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 112 2082
Size: 112 1970
BstYI r'GATC_y
Cuts at: 0 1436 1658 1970 2082
Size: 1436 222 312 112
Fragments arranged by size:
1436 312 222 112
Bsu36I CC'TnA_GG
Cuts at: 0 1432 2082
Size: 1432 650
BtgI C'CryG_G
Cuts at: 0 1262 1736 2082
Size: 1262 474 346
Fragments arranged by size:
1262 474 346
BtsI GCAGTG_nn'
Cuts at: 0 971 1234 2082
Size: 971 263 848
Fragments arranged by size:
971 848 263
DraIII CAC_nnn'GTG
Cuts at: 0 374 2082
Size: 374 1708
DrdI GACnn_nn'nnGTC
Cuts at: 0 1584 1765 1912 2082
Size: 1584 181 147 170
Fragments arranged by size:
1584 181 170 147
EaeI y'GGCC_r
Cuts at: 0 1464 2082
Size: 1464 618
EarI CTCTTCn'nnn_
Cuts at: 0 137 200 1130 1610 2082
Size: 137 63 930 480 472
Fragments arranged by size:
930 480 472 137 63
EcoICRI GAG'CTC
Cuts at: 0 725 2082
Size: 725 1357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 293 479 584 762 791 810 1276
Size: 70 223 186 105 178 29 19 466
Cuts at: 1276 1531 1567 2082
Size: 255 36 515
Fragments arranged by size:
515 466 255 223 186 178 105 70
36 29 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 836 1609 2082
Size: 836 773 473
Fragments arranged by size:
836 773 473
EcoO109I rG'GnC_Cy
Cuts at: 0 492 630 649 704 884 907 1251 2082
Size: 492 138 19 55 180 23 344 831
Fragments arranged by size:
831 492 344 180 138 55 23 19
EcoRI G'AATT_C
Cuts at: 0 1189 1846 2082
Size: 1189 657 236
Fragments arranged by size:
1189 657 236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1851 2082
Size: 1851 231
FspI TGC'GCA
Cuts at: 0 700 2082
Size: 700 1382
HaeII r_GCGC'y
Cuts at: 0 862 1992 2043 2082
Size: 862 1130 51 39
Fragments arranged by size:
1130 862 51 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 1204 1236 1484 1516 2082
Size: 646 32 526 32 248 32 566
Fragments arranged by size:
646 566 526 248 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1333 2082
Size: 1333 749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1005 1352 2082
Size: 16 989 347 730
Fragments arranged by size:
989 730 347 16
MscI TGG'CCA
Cuts at: 0 1466 2082
Size: 1466 616
MslI CAynn'nnrTG
Cuts at: 0 110 2082
Size: 110 1972
MspA1I CmG'CkG
Cuts at: 0 290 312 1084 1965 2082
Size: 290 22 772 881 117
Fragments arranged by size:
881 772 290 117 22
NcoI C'CATG_G
Cuts at: 0 1262 1736 2082
Size: 1262 474 346
Fragments arranged by size:
1262 474 346
NspI r_CATG'y
Cuts at: 0 1419 2082
Size: 1419 663
PasI CC'CwG_GG
Cuts at: 0 964 1176 2082
Size: 964 212 906
Fragments arranged by size:
964 906 212
PflMI CCAn_nnn'nTGG
Cuts at: 0 1024 2082
Size: 1024 1058
PmlI CAC'GTG
Cuts at: 0 2070 2082
Size: 2070 12
PpuMI rG'GwC_Cy
Cuts at: 0 492 884 907 2082
Size: 492 392 23 1175
Fragments arranged by size:
1175 492 392 23
PspOMI G'GGCC_C
Cuts at: 0 630 2082
Size: 630 1452
PspXI vC'TCGA_Gb
Cuts at: 0 188 605 2082
Size: 188 417 1477
Fragments arranged by size:
1477 417 188
PstI C_TGCA'G
Cuts at: 0 1083 1104 1229 2082
Size: 1083 21 125 853
Fragments arranged by size:
1083 853 125 21
PvuII CAG'CTG
Cuts at: 0 312 1084 1965 2082
Size: 312 772 881 117
Fragments arranged by size:
881 772 312 117
SacI G_AGCT'C
Cuts at: 0 727 2082
Size: 727 1355
SanDI GG'GwC_CC
Cuts at: 0 884 2082
Size: 884 1198
SapI GCTCTTCn'nnn_
Cuts at: 0 137 1610 2082
Size: 137 1473 472
Fragments arranged by size:
1473 472 137
SfcI C'TryA_G
Cuts at: 0 1079 1100 1225 2053 2082
Size: 1079 21 125 828 29
Fragments arranged by size:
1079 828 125 29 21
SmaI CCC'GGG
Cuts at: 0 595 635 672 2082
Size: 595 40 37 1410
Fragments arranged by size:
1410 595 40 37
SmlI C'TyrA_G
Cuts at: 0 188 605 1501 2082
Size: 188 417 896 581
Fragments arranged by size:
896 581 417 188
SrfI GCCC'GGGC
Cuts at: 0 595 2082
Size: 595 1487
StuI AGG'CCT
Cuts at: 0 823 1697 2082
Size: 823 874 385
Fragments arranged by size:
874 823 385
StyI C'CwwG_G
Cuts at: 0 1247 1262 1736 1979 2082
Size: 1247 15 474 243 103
Fragments arranged by size:
1247 474 243 103 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1055 1168 2082
Size: 1055 113 914
Fragments arranged by size:
1055 914 113
TatI w'GTAC_w
Cuts at: 0 1676 2082
Size: 1676 406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 221 253 266 298 2082
Size: 221 32 13 32 1784
Fragments arranged by size:
1784 221 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 0 905 2082
Size: 905 1177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 2082
Size: 378 1704
XhoI C'TCGA_G
Cuts at: 0 188 605 2082
Size: 188 417 1477
Fragments arranged by size:
1477 417 188
XmaI C'CCGG_G
Cuts at: 0 593 633 670 2082
Size: 593 40 37 1412
Fragments arranged by size:
1412 593 40 37
XmnI GAAnn'nnTTC
Cuts at: 0 1531 2082
Size: 1531 551
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BanI BanII BbsI BbvCI
BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrFI BsrGI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI
NspI PasI PflMI PmlI PpuMI PspOMI PspXI PstI
PvuII SacI SanDI SapI SfcI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaBI
BsiEI BsiWI BspHI BsrBI BsrDI BssHII BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI EagI EciI EcoRV
FseI FspAI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PfoI PmeI PpiI PshAI PsiI PsrI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
XbaI ZraI