Restriction map for
pF1KA0983
(Linear) (Six-base) MAPSORT of: KIAA0983.seq Check: 7348 from: 1 to: 2049
KIAA0983.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 181 797 1162 2049
Size: 181 616 365 887
Fragments arranged by size:
887 616 365 181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 235 1042 1075 1456 1968 2022 2049
Size: 83 152 807 33 381 512 54 27
Fragments arranged by size:
807 512 381 152 83 54 33 27
AhdI GACnn_n'nnGTC
Cuts at: 0 1182 2049
Size: 1182 867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1033 2049
Size: 1033 1016
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 248 280 2049
Size: 248 32 1769
Fragments arranged by size:
1769 248 32
AlwNI CAG_nnn'CTG
Cuts at: 0 192 396 1338 1884 2049
Size: 192 204 942 546 165
Fragments arranged by size:
942 546 204 192 165
ApaI G_GGCC'C
Cuts at: 0 1946 2049
Size: 1946 103
ApoI r'AATT_y
Cuts at: 0 1951 2049
Size: 1951 98
BamHI G'GATC_C
Cuts at: 0 263 2049
Size: 263 1786
BanI G'GyrC_C
Cuts at: 0 1028 2049
Size: 1028 1021
BanII G_rGCy'C
Cuts at: 0 57 1946 2049
Size: 57 1889 103
Fragments arranged by size:
1889 103 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 1444 2049
Size: 1444 605
BciVI GTATCCnnnnn_n'
Cuts at: 0 1223 2049
Size: 1223 826
BclI T'GATC_A
Cuts at: 0 1154 2049
Size: 1154 895
BlpI GC'TnA_GC
Cuts at: 0 356 1399 2049
Size: 356 1043 650
Fragments arranged by size:
1043 650 356
Bme1580I G_kGCm'C
Cuts at: 0 1678 1946 2049
Size: 1678 268 103
Fragments arranged by size:
1678 268 103
BmgBI CAC'GTC
Cuts at: 0 568 2049
Size: 568 1481
BmrI ACTGGGnnnn_n'
Cuts at: 0 585 994 2049
Size: 585 409 1055
Fragments arranged by size:
1055 585 409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 954 2049
Size: 954 1095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 604 670 893 1930 2049
Size: 373 231 66 223 1037 119
Fragments arranged by size:
1037 373 231 223 119 66
Bpu10I CC'TnA_GC
Cuts at: 0 676 2049
Size: 676 1373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1324 2049
Size: 631 693 725
Fragments arranged by size:
725 693 631
BsaI GGTCTCn'nnnn_
Cuts at: 0 536 969 1411 1906 2049
Size: 536 433 442 495 143
Fragments arranged by size:
536 495 442 433 143
BsaBI GATnn'nnATC
Cuts at: 0 374 2049
Size: 374 1675
BsaWI w'CCGG_w
Cuts at: 0 723 2049
Size: 723 1326
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 724 754 1954 1984 2049
Size: 436 30 258 30 1200 30 65
Fragments arranged by size:
1200 436 258 65 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 502 964 967 1710 2049
Size: 130 372 462 3 743 339
Fragments arranged by size:
743 462 372 339 130 3
BseYI C'CCAG_C
Cuts at: 0 57 1338 1370 1633 1849 2049
Size: 57 1281 32 263 216 200
Fragments arranged by size:
1281 263 216 200 57 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1190 1351 1468 1582 2049
Size: 748 442 161 117 114 467
Fragments arranged by size:
748 467 442 161 117 114
Bsp1286I G_dGCh'C
Cuts at: 0 57 1678 1946 2049
Size: 57 1621 268 103
Fragments arranged by size:
1621 268 103 57
BspHI T'CATG_A
Cuts at: 0 284 1100 1115 2049
Size: 284 816 15 934
Fragments arranged by size:
934 816 284 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 181 797 1162 2049
Size: 181 616 365 887
Fragments arranged by size:
887 616 365 181
BssSI C'ACGA_G
Cuts at: 0 349 2049
Size: 349 1700
BstEII G'GTnAC_C
Cuts at: 0 1312 2049
Size: 1312 737
BstXI CCAn_nnnn'nTGG
Cuts at: 0 441 1413 2049
Size: 441 972 636
Fragments arranged by size:
972 636 441
BstYI r'GATC_y
Cuts at: 0 129 203 263 2049
Size: 129 74 60 1786
Fragments arranged by size:
1786 129 74 60
Bsu36I CC'TnA_GG
Cuts at: 0 2027 2049
Size: 2027 22
BtgI C'CryG_G
Cuts at: 0 536 1145 1799 2049
Size: 536 609 654 250
Fragments arranged by size:
654 609 536 250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 62 2029 2049
Size: 62 1967 20
Fragments arranged by size:
1967 62 20
BtsI GCAGTG_nn'
Cuts at: 0 824 882 2049
Size: 824 58 1167
Fragments arranged by size:
1167 824 58
ClaI AT'CG_AT
Cuts at: 0 78 2049
Size: 78 1971
DraI TTT'AAA
Cuts at: 0 2013 2049
Size: 2013 36
EarI CTCTTCn'nnn_
Cuts at: 0 211 330 1018 1534 1987 2049
Size: 211 119 688 516 453 62
Fragments arranged by size:
688 516 453 211 119 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 235 373 604 670 893 1042 1075
Size: 83 152 138 231 66 223 149 33
Cuts at: 1075 1456 1930 1968 2022 2049
Size: 381 474 38 54 27
Fragments arranged by size:
474 381 231 223 152 149 138 83
66 54 38 33 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 421 2049
Size: 421 1628
EcoO109I rG'GnC_Cy
Cuts at: 0 138 1942 1990 2049
Size: 138 1804 48 59
Fragments arranged by size:
1804 138 59 48
EcoRV GAT'ATC
Cuts at: 0 516 2049
Size: 516 1533
HaeII r_GCGC'y
Cuts at: 0 972 1406 1663 2049
Size: 972 434 257 386
Fragments arranged by size:
972 434 386 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 610 642 1168 1200 2049
Size: 436 32 142 32 526 32 849
Fragments arranged by size:
849 526 436 142 32 32 32
HincII GTy'rAC
Cuts at: 0 1865 2049
Size: 1865 184
HindIII A'AGCT_T
Cuts at: 0 298 2049
Size: 298 1751
MfeI C'AATT_G
Cuts at: 0 1571 2049
Size: 1571 478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 2049
Size: 977 1072
MspA1I CmG'CkG
Cuts at: 0 61 591 1696 1860 1875 1881 2049
Size: 61 530 1105 164 15 6 168
Fragments arranged by size:
1105 530 168 164 61 15 6
NcoI C'CATG_G
Cuts at: 0 536 1145 1799 2049
Size: 536 609 654 250
Fragments arranged by size:
654 609 536 250
PpuMI rG'GwC_Cy
Cuts at: 0 138 1990 2049
Size: 138 1852 59
Fragments arranged by size:
1852 138 59
PsiI TTA'TAA
Cuts at: 0 497 2049
Size: 497 1552
PspOMI G'GGCC_C
Cuts at: 0 1942 2049
Size: 1942 107
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1699 1731 2049
Size: 1699 32 318
Fragments arranged by size:
1699 318 32
PstI C_TGCA'G
Cuts at: 0 386 1691 1880 2049
Size: 386 1305 189 169
Fragments arranged by size:
1305 386 189 169
PvuII CAG'CTG
Cuts at: 0 61 1860 1875 2049
Size: 61 1799 15 174
Fragments arranged by size:
1799 174 61 15
SanDI GG'GwC_CC
Cuts at: 0 138 2049
Size: 138 1911
SapI GCTCTTCn'nnn_
Cuts at: 0 211 1987 2049
Size: 211 1776 62
Fragments arranged by size:
1776 211 62
SfcI C'TryA_G
Cuts at: 0 108 184 382 418 1687 1876 2049
Size: 108 76 198 36 1269 189 173
Fragments arranged by size:
1269 198 189 173 108 76 36
SmlI C'TyrA_G
Cuts at: 0 646 1303 2049
Size: 646 657 746
Fragments arranged by size:
746 657 646
StuI AGG'CCT
Cuts at: 0 841 2049
Size: 841 1208
StyI C'CwwG_G
Cuts at: 0 536 890 1145 1538 1799 1843 1946 2049
Size: 536 354 255 393 261 44 103 103
Fragments arranged by size:
536 393 354 261 255 103 103 44
SwaI ATTT'AAAT
Cuts at: 0 2013 2049
Size: 2013 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1046 1317 1330 1554 1930 2049
Size: 1046 271 13 224 376 119
Fragments arranged by size:
1046 376 271 224 119 13
TatI w'GTAC_w
Cuts at: 0 167 1299 2049
Size: 167 1132 750
Fragments arranged by size:
1132 750 167
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1721 1753 1954 1986 2049
Size: 1721 32 201 32 63
Fragments arranged by size:
1721 201 63 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1195 2049
Size: 1195 854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1614 2049
Size: 1614 435
Enzymes that do cut:
AarI AcuI AhdI AlfI AloI AlwNI ApaI ApoI
BamHI BanI BanII BbsI BciVI BclI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspHI BspMI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI EarI Eco57MI EcoNI EcoO109I EcoRV HaeII
Hin4I HincII HindIII MfeI MmeI MspA1I NcoI PpuMI
PsiI PspOMI PsrI PstI PvuII SanDI SapI SfcI
SmlI StuI StyI SwaI TaqII TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BfrBI BglI BglII BmtI BsaAI
BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoRI FalI
FseI FspI FspAI HpaI KasI KpnI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspXI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI XbaI XhoI
XmaI XmnI ZraI