Restriction map for
pF1KSDA0979
(Linear) (Six-base) MAPSORT of: KIAA0979.seq Check: 7536 from: 1 to: 4341
KIAA0979.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AccI GT'mk_AC
Cuts at: 0 533 4341
Size: 533 3808
Acc65I G'GTAC_C
Cuts at: 0 609 4341
Size: 609 3732
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 176 299 685 1748 2938 3752 4341
Size: 167 9 123 386 1063 1190 814 589
Fragments arranged by size:
1190 1063 814 589 386 167 123 9
AflII C'TTAA_G
Cuts at: 0 1957 2550 4341
Size: 1957 593 1791
Fragments arranged by size:
1957 1791 593
AleI CACnn'nnGTG
Cuts at: 0 1651 4341
Size: 1651 2690
AlwNI CAG_nnn'CTG
Cuts at: 0 720 1273 2059 3775 4341
Size: 720 553 786 1716 566
Fragments arranged by size:
1716 786 720 566 553
ApaLI G'TGCA_C
Cuts at: 0 1831 4341
Size: 1831 2510
ApoI r'AATT_y
Cuts at: 0 455 624 832 1003 1241 1602 1642 2069
Size: 455 169 208 171 238 361 40 427
Cuts at: 2069 2474 3309 4341
Size: 405 835 1032
Fragments arranged by size:
1032 835 455 427 405 361 238 208
171 169 40
AseI AT'TA_AT
Cuts at: 0 3082 4341
Size: 3082 1259
BanI G'GyrC_C
Cuts at: 0 609 4341
Size: 609 3732
BanII G_rGCy'C
Cuts at: 0 367 547 785 2868 4341
Size: 367 180 238 2083 1473
Fragments arranged by size:
2083 1473 367 238 180
BbsI GAAGACnn'nnnn_
Cuts at: 0 3560 4134 4341
Size: 3560 574 207
Fragments arranged by size:
3560 574 207
BciVI GTATCCnnnnn_n'
Cuts at: 0 348 582 2206 4341
Size: 348 234 1624 2135
Fragments arranged by size:
2135 1624 348 234
BclI T'GATC_A
Cuts at: 0 1158 1799 1926 2334 4341
Size: 1158 641 127 408 2007
Fragments arranged by size:
2007 1158 641 408 127
BglI GCCn_nnn'nGGC
Cuts at: 0 942 4341
Size: 942 3399
BglII A'GATC_T
Cuts at: 0 2385 4341
Size: 2385 1956
Bme1580I G_kGCm'C
Cuts at: 0 1835 2832 4341
Size: 1835 997 1509
Fragments arranged by size:
1835 1509 997
BmgBI CAC'GTC
Cuts at: 0 2629 4341
Size: 2629 1712
BmrI ACTGGGnnnn_n'
Cuts at: 0 2220 4341
Size: 2220 2121
BmtI G_CTAG'C
Cuts at: 0 1013 4341
Size: 1013 3328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 692 2882 4341
Size: 692 2190 1459
Fragments arranged by size:
2190 1459 692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3947 4341
Size: 3947 394
Bpu10I CC'TnA_GC
Cuts at: 0 1625 4341
Size: 1625 2716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 415 662 2830 4341
Size: 190 225 247 2168 1511
Fragments arranged by size:
2168 1511 247 225 190
BsaI GGTCTCn'nnnn_
Cuts at: 0 2215 4341
Size: 2215 2126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 874 3691 3988 3991 4297 4300 4341
Size: 100 774 2817 297 3 306 3 41
Fragments arranged by size:
2817 774 306 297 100 41 3 3
BseYI C'CCAG_C
Cuts at: 0 464 824 2644 4341
Size: 464 360 1820 1697
Fragments arranged by size:
1820 1697 464 360
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2045 4341
Size: 2045 2296
BsiHKAI G_wGCw'C
Cuts at: 0 547 785 1835 1860 2868 4056 4341
Size: 547 238 1050 25 1008 1188 285
Fragments arranged by size:
1188 1050 1008 547 285 238 25
BsmI GAATG_Cn'
Cuts at: 0 2746 4341
Size: 2746 1595
BsmBI CGTCTCn'nnnn_
Cuts at: 0 90 3596 4341
Size: 90 3506 745
Fragments arranged by size:
3506 745 90
Bsp1286I G_dGCh'C
Cuts at: 0 367 547 785 1835 1860 2832 2868 4056
Size: 367 180 238 1050 25 972 36 1188
Cuts at: 4056 4341
Size: 285
Fragments arranged by size:
1188 1050 972 367 285 238 180 36
25
BspHI T'CATG_A
Cuts at: 0 1022 1634 2393 4341
Size: 1022 612 759 1948
Fragments arranged by size:
1948 1022 759 612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 621 950 1933 4341
Size: 621 329 983 2408
Fragments arranged by size:
2408 983 621 329
BsrDI GCAATG_nn'
Cuts at: 0 401 456 855 2884 3474 4341
Size: 401 55 399 2029 590 867
Fragments arranged by size:
2029 867 590 401 399 55
BsrGI T'GTAC_A
Cuts at: 0 3194 4341
Size: 3194 1147
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2059 2827 4341
Size: 2059 768 1514
Fragments arranged by size:
2059 1514 768
BstYI r'GATC_y
Cuts at: 0 1370 2265 2385 2844 4341
Size: 1370 895 120 459 1497
Fragments arranged by size:
1497 1370 895 459 120
Bsu36I CC'TnA_GG
Cuts at: 0 1085 3794 4341
Size: 1085 2709 547
Fragments arranged by size:
2709 1085 547
BtsI GCAGTG_nn'
Cuts at: 0 952 3793 4341
Size: 952 2841 548
Fragments arranged by size:
2841 952 548
DraI TTT'AAA
Cuts at: 0 170 629 837 1457 4341
Size: 170 459 208 620 2884
Fragments arranged by size:
2884 620 459 208 170
DraIII CAC_nnn'GTG
Cuts at: 0 3203 4041 4341
Size: 3203 838 300
Fragments arranged by size:
3203 838 300
DrdI GACnn_nn'nnGTC
Cuts at: 0 2168 4341
Size: 2168 2173
EaeI y'GGCC_r
Cuts at: 0 501 4341
Size: 501 3840
EarI CTCTTCn'nnn_
Cuts at: 0 661 2093 2856 2960 3905 4341
Size: 661 1432 763 104 945 436
Fragments arranged by size:
1432 945 763 661 436 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 35 976 3839 4341
Size: 35 941 2863 502
Fragments arranged by size:
2863 941 502 35
EcoICRI GAG'CTC
Cuts at: 0 545 783 2866 4341
Size: 545 238 2083 1475
Fragments arranged by size:
2083 1475 545 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 176 299 685 1748 2938 3752 3947
Size: 167 9 123 386 1063 1190 814 195
Cuts at: 3947 4341
Size: 394
Fragments arranged by size:
1190 1063 814 394 386 195 167 123
9
EcoO109I rG'GnC_Cy
Cuts at: 0 2161 4341
Size: 2161 2180
EcoRV GAT'ATC
Cuts at: 0 972 4341
Size: 972 3369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1827 3076 4341
Size: 1827 1249 1265
Fragments arranged by size:
1827 1265 1249
HaeII r_GCGC'y
Cuts at: 0 867 4341
Size: 867 3474
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 127 159 258 290 556 588
Size: 61 32 34 32 99 32 266 32
Cuts at: 588 1362 1394 1831 1863 2250 2282 4341
Size: 774 32 437 32 387 32 2059
Fragments arranged by size:
2059 774 437 387 266 99 61 34
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3402 4341
Size: 3402 939
HpaI GTT'AAC
Cuts at: 0 3402 4341
Size: 3402 939
KpnI G_GTAC'C
Cuts at: 0 613 4341
Size: 613 3728
MfeI C'AATT_G
Cuts at: 0 4088 4341
Size: 4088 253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1530 1729 1945 3727 4341
Size: 1530 199 216 1782 614
Fragments arranged by size:
1782 1530 614 216 199
MscI TGG'CCA
Cuts at: 0 503 4341
Size: 503 3838
MslI CAynn'nnrTG
Cuts at: 0 1651 2579 4341
Size: 1651 928 1762
Fragments arranged by size:
1762 1651 928
MspA1I CmG'CkG
Cuts at: 0 1129 1273 2932 4341
Size: 1129 144 1659 1409
Fragments arranged by size:
1659 1409 1129 144
NdeI CA'TA_TG
Cuts at: 0 427 521 642 4341
Size: 427 94 121 3699
Fragments arranged by size:
3699 427 121 94
NheI G'CTAG_C
Cuts at: 0 1009 4341
Size: 1009 3332
NspI r_CATG'y
Cuts at: 0 763 1712 2929 4341
Size: 763 949 1217 1412
Fragments arranged by size:
1412 1217 949 763
PfoI T'CCnGG_A
Cuts at: 0 1790 3363 3498 4341
Size: 1790 1573 135 843
Fragments arranged by size:
1790 1573 843 135
PpuMI rG'GwC_Cy
Cuts at: 0 2161 4341
Size: 2161 2180
PshAI GACnn'nnGTC
Cuts at: 0 2467 4341
Size: 2467 1874
PsiI TTA'TAA
Cuts at: 0 328 493 1247 4341
Size: 328 165 754 3094
Fragments arranged by size:
3094 754 328 165
PvuII CAG'CTG
Cuts at: 0 1129 1273 2932 4341
Size: 1129 144 1659 1409
Fragments arranged by size:
1659 1409 1129 144
SacI G_AGCT'C
Cuts at: 0 547 785 2868 4341
Size: 547 238 2083 1473
Fragments arranged by size:
2083 1473 547 238
SapI GCTCTTCn'nnn_
Cuts at: 0 2856 3905 4341
Size: 2856 1049 436
Fragments arranged by size:
2856 1049 436
ScaI AGT'ACT
Cuts at: 0 1694 3240 4341
Size: 1694 1546 1101
Fragments arranged by size:
1694 1546 1101
SfcI C'TryA_G
Cuts at: 0 1409 4298 4341
Size: 1409 2889 43
Fragments arranged by size:
2889 1409 43
SmlI C'TyrA_G
Cuts at: 0 205 394 677 1957 2550 2809 4341
Size: 205 189 283 1280 593 259 1532
Fragments arranged by size:
1532 1280 593 283 259 205 189
SphI G_CATG'C
Cuts at: 0 2929 4341
Size: 2929 1412
SspI AAT'ATT
Cuts at: 0 792 2570 3080 3138 4341
Size: 792 1778 510 58 1203
Fragments arranged by size:
1778 1203 792 510 58
StuI AGG'CCT
Cuts at: 0 2786 4341
Size: 2786 1555
StyI C'CwwG_G
Cuts at: 0 3159 4341
Size: 3159 1182
SwaI ATTT'AAAT
Cuts at: 0 837 1457 4341
Size: 837 620 2884
Fragments arranged by size:
2884 837 620
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 478 4341
Size: 478 3863
TatI w'GTAC_w
Cuts at: 0 1692 3021 3194 3238 4341
Size: 1692 1329 173 44 1103
Fragments arranged by size:
1692 1329 1103 173 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3576 3608 4341
Size: 3576 32 733
Fragments arranged by size:
3576 733 32
XmnI GAAnn'nnTTC
Cuts at: 0 1642 4341
Size: 1642 2699
Enzymes that do cut:
AccI Acc65I AcuI AflII AleI AlwNI ApaLI ApoI
AseI BanI BanII BbsI BciVI BclI BglI BglII
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrGI BstAPI BstYI Bsu36I BtsI
DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I HincII HpaI KpnI
MfeI MmeI MscI MslI MspA1I NdeI NheI NspI
PfoI PpuMI PshAI PsiI PvuII SacI SapI ScaI
SfcI SmlI SphI SspI StuI StyI SwaI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BfrBI BlpI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BspEI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI EagI EcoNI EcoRI FseI FspI FspAI HindIII
KasI MluI NaeI NarI NcoI NgoMIV NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI Tth111I XbaI XcmI XhoI XmaI
ZraI