Restriction map for
pF1KSDA0977
(Linear) (Six-base) MAPSORT of: KIAA0977.seq Check: 7636 from: 1 to: 3498
KIAA0977.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 1172 2966 3037 3498
Size: 86 1086 1794 71 461
Fragments arranged by size:
1794 1086 461 86 71
AflII C'TTAA_G
Cuts at: 0 2046 2868 3498
Size: 2046 822 630
Fragments arranged by size:
2046 822 630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1280 3498
Size: 1280 2218
AlwNI CAG_nnn'CTG
Cuts at: 0 109 3498
Size: 109 3389
ApaI G_GGCC'C
Cuts at: 0 1657 3498
Size: 1657 1841
ApaLI G'TGCA_C
Cuts at: 0 263 1260 2147 3498
Size: 263 997 887 1351
Fragments arranged by size:
1351 997 887 263
ApoI r'AATT_y
Cuts at: 0 331 1059 2080 2196 2634 2668 3199 3264
Size: 331 728 1021 116 438 34 531 65
Cuts at: 3264 3498
Size: 234
Fragments arranged by size:
1021 728 531 438 331 234 116 65
34
AseI AT'TA_AT
Cuts at: 0 452 3498
Size: 452 3046
AvaI C'yCGr_G
Cuts at: 0 2678 3498
Size: 2678 820
BanI G'GyrC_C
Cuts at: 0 60 2095 3037 3498
Size: 60 2035 942 461
Fragments arranged by size:
2035 942 461 60
BanII G_rGCy'C
Cuts at: 0 885 1657 2554 3498
Size: 885 772 897 944
Fragments arranged by size:
944 897 885 772
BbsI GAAGACnn'nnnn_
Cuts at: 0 478 1715 3025 3187 3498
Size: 478 1237 1310 162 311
Fragments arranged by size:
1310 1237 478 311 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3365 3399 3498
Size: 3365 34 99
Fragments arranged by size:
3365 99 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3012 3498
Size: 3012 486
BclI T'GATC_A
Cuts at: 0 2385 3498
Size: 2385 1113
BfrBI ATG'CAT
Cuts at: 0 499 2397 3498
Size: 499 1898 1101
Fragments arranged by size:
1898 1101 499
BlpI GC'TnA_GC
Cuts at: 0 1039 3498
Size: 1039 2459
Bme1580I G_kGCm'C
Cuts at: 0 267 771 1264 1306 1657 2151 2540 3042
Size: 267 504 493 42 351 494 389 502
Cuts at: 3042 3498
Size: 456
Fragments arranged by size:
504 502 494 493 456 389 351 267
42
BmrI ACTGGGnnnn_n'
Cuts at: 0 1889 2534 3498
Size: 1889 645 964
Fragments arranged by size:
1889 964 645
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 899 1305 3498
Size: 899 406 2193
Fragments arranged by size:
2193 899 406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1160 3143 3498
Size: 56 1104 1983 355
Fragments arranged by size:
1983 1104 355 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2058 3039 3498
Size: 2058 981 459
Fragments arranged by size:
2058 981 459
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 746 1788 3498
Size: 73 673 1042 1710
Fragments arranged by size:
1710 1042 673 73
BsaHI Gr'CG_yC
Cuts at: 0 29 3498
Size: 29 3469
BsaWI w'CCGG_w
Cuts at: 0 2847 3498
Size: 2847 651
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2847 2877 3498
Size: 2847 30 621
Fragments arranged by size:
2847 621 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1222 2948 3498
Size: 1222 1726 550
Fragments arranged by size:
1726 1222 550
BseYI C'CCAG_C
Cuts at: 0 33 2704 3498
Size: 33 2671 794
Fragments arranged by size:
2671 794 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1919 3233 3498
Size: 1919 1314 265
Fragments arranged by size:
1919 1314 265
BsiEI CG_ry'CG
Cuts at: 0 9 3498
Size: 9 3489
BsiHKAI G_wGCw'C
Cuts at: 0 267 1264 2151 2554 3498
Size: 267 997 887 403 944
Fragments arranged by size:
997 944 887 403 267
Bsp1286I G_dGCh'C
Cuts at: 0 267 771 885 1264 1306 1657 2151 2540
Size: 267 504 114 379 42 351 494 389
Cuts at: 2540 2554 3042 3498
Size: 14 488 456
Fragments arranged by size:
504 494 488 456 389 379 351 267
114 42 14
BspEI T'CCGG_A
Cuts at: 0 2847 3498
Size: 2847 651
BspHI T'CATG_A
Cuts at: 0 2421 3498
Size: 2421 1077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1015 2595 3498
Size: 1015 1580 903
Fragments arranged by size:
1580 1015 903
BsrDI GCAATG_nn'
Cuts at: 0 1515 1614 3498
Size: 1515 99 1884
Fragments arranged by size:
1884 1515 99
BssSI C'ACGA_G
Cuts at: 0 2279 3498
Size: 2279 1219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2535 3498
Size: 2535 963
BstEII G'GTnAC_C
Cuts at: 0 3414 3498
Size: 3414 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 978 1278 2337 3147 3498
Size: 978 300 1059 810 351
Fragments arranged by size:
1059 978 810 351 300
BstYI r'GATC_y
Cuts at: 0 2118 2136 3498
Size: 2118 18 1362
Fragments arranged by size:
2118 1362 18
Bsu36I CC'TnA_GG
Cuts at: 0 1205 1316 3498
Size: 1205 111 2182
Fragments arranged by size:
2182 1205 111
BtgI C'CryG_G
Cuts at: 0 1538 2567 3498
Size: 1538 1029 931
Fragments arranged by size:
1538 1029 931
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 485 658 3498
Size: 485 173 2840
Fragments arranged by size:
2840 485 173
BtsI GCAGTG_nn'
Cuts at: 0 125 886 1030 1262 1264 2201 2488 3498
Size: 125 761 144 232 2 937 287 1010
Fragments arranged by size:
1010 937 761 287 232 144 125 2
ClaI AT'CG_AT
Cuts at: 0 299 3498
Size: 299 3199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1497 1532 3498
Size: 1497 35 1966
Fragments arranged by size:
1966 1497 35
DraI TTT'AAA
Cuts at: 0 383 2673 3498
Size: 383 2290 825
Fragments arranged by size:
2290 825 383
DraIII CAC_nnn'GTG
Cuts at: 0 1588 2751 3136 3498
Size: 1588 1163 385 362
Fragments arranged by size:
1588 1163 385 362
EaeI y'GGCC_r
Cuts at: 0 6 3492 3498
Size: 6 3486 6
Fragments arranged by size:
3486 6 6
EagI C'GGCC_G
Cuts at: 0 6 3498
Size: 6 3492
EarI CTCTTCn'nnn_
Cuts at: 0 105 868 1008 1364 2477 2725 3498
Size: 105 763 140 356 1113 248 773
Fragments arranged by size:
1113 773 763 356 248 140 105
EcoICRI GAG'CTC
Cuts at: 0 2552 3498
Size: 2552 946
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 86 1160 1172 2966 3037 3143 3498
Size: 56 30 1074 12 1794 71 106 355
Fragments arranged by size:
1794 1074 355 106 71 56 30 12
EcoRI G'AATT_C
Cuts at: 0 1059 2080 3264 3498
Size: 1059 1021 1184 234
Fragments arranged by size:
1184 1059 1021 234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2140 3498
Size: 2140 1358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 474 506 565 594 597 626
Size: 292 32 150 32 59 29 3 29
Cuts at: 626 1198 1230 1480 1512 1783 1815 2344 2376
Size: 572 32 250 32 271 32 529 32
Cuts at: 2376 2847 2879 3280 3312 3498
Size: 471 32 401 32 186
Fragments arranged by size:
572 529 471 401 292 271 250 186
150 59 32 32 32 32 32 32
32 32 29 29 3
HincII GTy'rAC
Cuts at: 0 654 1626 3498
Size: 654 972 1872
Fragments arranged by size:
1872 972 654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 377 839 869 3177 3307 3498
Size: 341 36 462 30 2308 130 191
Fragments arranged by size:
2308 462 341 191 130 36 30
MslI CAynn'nnrTG
Cuts at: 0 976 1124 1780 2156 2420 2426 3307 3498
Size: 976 148 656 376 264 6 881 191
Fragments arranged by size:
976 881 656 376 264 191 148 6
MspA1I CmG'CkG
Cuts at: 0 76 106 313 2764 3498
Size: 76 30 207 2451 734
Fragments arranged by size:
2451 734 207 76 30
NcoI C'CATG_G
Cuts at: 0 1538 2567 3498
Size: 1538 1029 931
Fragments arranged by size:
1538 1029 931
NsiI A_TGCA'T
Cuts at: 0 501 2399 3498
Size: 501 1898 1099
Fragments arranged by size:
1898 1099 501
NspI r_CATG'y
Cuts at: 0 499 1050 2397 2401 3498
Size: 499 551 1347 4 1097
Fragments arranged by size:
1347 1097 551 499 4
PasI CC'CwG_GG
Cuts at: 0 1237 1306 3498
Size: 1237 69 2192
Fragments arranged by size:
2192 1237 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 1165 3220 3289 3498
Size: 1165 2055 69 209
Fragments arranged by size:
2055 1165 209 69
PfoI T'CCnGG_A
Cuts at: 0 938 3498
Size: 938 2560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1011 1043 3303 3335 3498
Size: 1011 32 2260 32 163
Fragments arranged by size:
2260 1011 163 32 32
PsiI TTA'TAA
Cuts at: 0 2315 3498
Size: 2315 1183
PspOMI G'GGCC_C
Cuts at: 0 1653 3498
Size: 1653 1845
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1154 1186 3498
Size: 1154 32 2312
Fragments arranged by size:
2312 1154 32
PstI C_TGCA'G
Cuts at: 0 1037 1056 2763 3498
Size: 1037 19 1707 735
Fragments arranged by size:
1707 1037 735 19
PvuII CAG'CTG
Cuts at: 0 76 106 313 2764 3498
Size: 76 30 207 2451 734
Fragments arranged by size:
2451 734 207 76 30
SacI G_AGCT'C
Cuts at: 0 2554 3498
Size: 2554 944
SapI GCTCTTCn'nnn_
Cuts at: 0 2477 3498
Size: 2477 1021
SfcI C'TryA_G
Cuts at: 0 116 1033 1052 2177 2759 3086 3498
Size: 116 917 19 1125 582 327 412
Fragments arranged by size:
1125 917 582 412 327 116 19
SmlI C'TyrA_G
Cuts at: 0 2046 2073 2868 3054 3498
Size: 2046 27 795 186 444
Fragments arranged by size:
2046 795 444 186 27
SphI G_CATG'C
Cuts at: 0 2401 3498
Size: 2401 1097
SspI AAT'ATT
Cuts at: 0 1425 2655 3498
Size: 1425 1230 843
Fragments arranged by size:
1425 1230 843
StuI AGG'CCT
Cuts at: 0 948 3498
Size: 948 2550
StyI C'CwwG_G
Cuts at: 0 56 1503 1538 2336 2567 3498
Size: 56 1447 35 798 231 931
Fragments arranged by size:
1447 931 798 231 56 35
SwaI ATTT'AAAT
Cuts at: 0 2673 3498
Size: 2673 825
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 882 2920 3498
Size: 882 2038 578
Fragments arranged by size:
2038 882 578
XmnI GAAnn'nnTTC
Cuts at: 0 1229 3498
Size: 1229 2269
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApaI ApaLI ApoI AseI
AvaI BanI BanII BbsI BcgI BciVI BclI BfrBI
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrDI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII EaeI EagI EarI EcoICRI Eco57MI EcoRI FalI
Hin4I HincII MmeI MslI MspA1I NcoI NsiI NspI
PasI PflMI PfoI PpiI PsiI PspOMI PsrI PstI
PvuII SacI SapI SfcI SmlI SphI SspI StuI
StyI SwaI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BglI BglII BmgBI BmtI Bpu10I BsaAI
BsaBI BsiWI BsmI BsmBI BsrBI BsrFI BsrGI BssHII
BstBI BstZ17I DrdI EciI EcoNI EcoO109I EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PpuMI PshAI PspXI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI