Restriction map for
pF1KSDA0969
(Linear) (Six-base) MAPSORT of: KIAA0969.seq Check: 8431 from: 1 to: 3144
KIAA0969.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 2612 3144
Size: 2612 532
Acc65I G'GTAC_C
Cuts at: 0 981 3144
Size: 981 2163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 937 1013 3144
Size: 396 541 76 2131
Fragments arranged by size:
2131 541 396 76
AfeI AGC'GCT
Cuts at: 0 142 238 3144
Size: 142 96 2906
Fragments arranged by size:
2906 142 96
AflIII A'CryG_T
Cuts at: 0 371 3144
Size: 371 2773
AleI CACnn'nnGTG
Cuts at: 0 2495 3144
Size: 2495 649
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1984 2016 3144
Size: 1984 32 1128
Fragments arranged by size:
1984 1128 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1713 3144
Size: 1713 1431
ApaI G_GGCC'C
Cuts at: 0 769 1164 3144
Size: 769 395 1980
Fragments arranged by size:
1980 769 395
AvaI C'yCGr_G
Cuts at: 0 427 470 620 760 1858 2003 2678 3144
Size: 427 43 150 140 1098 145 675 466
Fragments arranged by size:
1098 675 466 427 150 145 140 43
BamHI G'GATC_C
Cuts at: 0 446 1268 1961 3144
Size: 446 822 693 1183
Fragments arranged by size:
1183 822 693 446
BanI G'GyrC_C
Cuts at: 0 981 2489 2898 3115 3144
Size: 981 1508 409 217 29
Fragments arranged by size:
1508 981 409 217 29
BanII G_rGCy'C
Cuts at: 0 427 769 940 1164 1235 1390 2116 2137
Size: 427 342 171 224 71 155 726 21
Cuts at: 2137 2174 2387 2720 2753 2821 3144
Size: 37 213 333 33 68 323
Fragments arranged by size:
726 427 342 333 323 224 213 171
155 71 68 37 33 21
BbeI G_GCGC'C
Cuts at: 0 3119 3144
Size: 3119 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1001 3144
Size: 1001 2143
BbvCI CC'TCA_GC
Cuts at: 0 1984 2046 2083 3144
Size: 1984 62 37 1061
Fragments arranged by size:
1984 1061 62 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 108 142 3144
Size: 108 34 3002
Fragments arranged by size:
3002 108 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 307 3144
Size: 307 2837
BglI GCCn_nnn'nGGC
Cuts at: 0 672 2259 2545 2641 2658 3144
Size: 672 1587 286 96 17 486
Fragments arranged by size:
1587 672 486 286 96 17
BglII A'GATC_T
Cuts at: 0 1952 3144
Size: 1952 1192
BlpI GC'TnA_GC
Cuts at: 0 383 3144
Size: 383 2761
Bme1580I G_kGCm'C
Cuts at: 0 506 769 1164 1370 1404 1437 2011 2255
Size: 506 263 395 206 34 33 574 244
Cuts at: 2255 2315 2903 2933 3144
Size: 60 588 30 211
Fragments arranged by size:
588 574 506 395 263 244 211 206
60 34 33 30
BmgBI CAC'GTC
Cuts at: 0 1971 2733 2928 3144
Size: 1971 762 195 216
Fragments arranged by size:
1971 762 216 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 884 1062 1424 2552 3144
Size: 884 178 362 1128 592
Fragments arranged by size:
1128 884 592 362 178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 753 2934 3144
Size: 753 2181 210
Fragments arranged by size:
2181 753 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 470 1186 1735 1977 2647 2744 2818
Size: 65 405 716 549 242 670 97 74
Cuts at: 2818 3065 3144
Size: 247 79
Fragments arranged by size:
716 670 549 405 247 242 97 79
74 65
Bpu10I CC'TnA_GC
Cuts at: 0 319 596 602 1984 2046 2083 2110 2815
Size: 319 277 6 1382 62 37 27 705
Cuts at: 2815 3144
Size: 329
Fragments arranged by size:
1382 705 329 319 277 62 37 27
6
BsaI GGTCTCn'nnnn_
Cuts at: 0 1875 3144
Size: 1875 1269
BsaBI GATnn'nnATC
Cuts at: 0 1082 2977 3144
Size: 1082 1895 167
Fragments arranged by size:
1895 1082 167
BsaHI Gr'CG_yC
Cuts at: 0 3116 3144
Size: 3116 28
BsaWI w'CCGG_w
Cuts at: 0 696 726 3144
Size: 696 30 2418
Fragments arranged by size:
2418 696 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1984 2014 3144
Size: 1984 30 1130
Fragments arranged by size:
1984 1130 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 1053 2045 2391 2479 2535 2662 2839
Size: 445 608 992 346 88 56 127 177
Cuts at: 2839 3068 3144
Size: 229 76
Fragments arranged by size:
992 608 445 346 229 177 127 88
76 56
BseYI C'CCAG_C
Cuts at: 0 91 556 717 800 926 1378 1599 2720
Size: 91 465 161 83 126 452 221 1121
Cuts at: 2720 3144
Size: 424
Fragments arranged by size:
1121 465 452 424 221 161 126 91
83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1672 1864 3144
Size: 361 1311 192 1280
Fragments arranged by size:
1311 1280 361 192
BsiEI CG_ry'CG
Cuts at: 0 1111 2999 3144
Size: 1111 1888 145
Fragments arranged by size:
1888 1111 145
BsiHKAI G_wGCw'C
Cuts at: 0 847 940 1856 2753 3144
Size: 847 93 916 897 391
Fragments arranged by size:
916 897 847 391 93
BsmI GAATG_Cn'
Cuts at: 0 2379 2486 3144
Size: 2379 107 658
Fragments arranged by size:
2379 658 107
BsmBI CGTCTCn'nnnn_
Cuts at: 0 863 3144
Size: 863 2281
Bsp1286I G_dGCh'C
Cuts at: 0 427 506 769 847 940 1164 1235 1370
Size: 427 79 263 78 93 224 71 135
Cuts at: 1370 1390 1404 1437 1856 2011 2116 2137 2174
Size: 20 14 33 419 155 105 21 37
Cuts at: 2174 2255 2315 2387 2720 2753 2821 2903 2933
Size: 81 60 72 333 33 68 82 30
Cuts at: 2933 3144
Size: 211
Fragments arranged by size:
427 419 333 263 224 211 155 135
105 93 82 81 79 78 72 71
68 60 37 33 33 30 21 20
14
BsrBI CCG'CTC
Cuts at: 0 87 1355 1373 1424 1446 3144
Size: 87 1268 18 51 22 1698
Fragments arranged by size:
1698 1268 87 51 22 18
BsrDI GCAATG_nn'
Cuts at: 0 1197 2215 2870 3040 3144
Size: 1197 1018 655 170 104
Fragments arranged by size:
1197 1018 655 170 104
BsrFI r'CCGG_y
Cuts at: 0 833 884 1131 1643 1934 3144
Size: 833 51 247 512 291 1210
Fragments arranged by size:
1210 833 512 291 247 51
BssSI C'ACGA_G
Cuts at: 0 2605 3144
Size: 2605 539
BstEII G'GTnAC_C
Cuts at: 0 2346 3144
Size: 2346 798
BstXI CCAn_nnnn'nTGG
Cuts at: 0 808 3144
Size: 808 2336
BstYI r'GATC_y
Cuts at: 0 446 479 1268 1952 1961 3144
Size: 446 33 789 684 9 1183
Fragments arranged by size:
1183 789 684 446 33 9
Bsu36I CC'TnA_GG
Cuts at: 0 2822 3144
Size: 2822 322
BtgI C'CryG_G
Cuts at: 0 455 1820 3109 3144
Size: 455 1365 1289 35
Fragments arranged by size:
1365 1289 455 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 111 3144
Size: 111 3033
BtsI GCAGTG_nn'
Cuts at: 0 232 344 964 1467 1771 2366 3144
Size: 232 112 620 503 304 595 778
Fragments arranged by size:
778 620 595 503 304 232 112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1179 1214 3144
Size: 1179 35 1930
Fragments arranged by size:
1930 1179 35
DraIII CAC_nnn'GTG
Cuts at: 0 1365 1432 3144
Size: 1365 67 1712
Fragments arranged by size:
1712 1365 67
EaeI y'GGCC_r
Cuts at: 0 636 835 1108 2072 2537 2566 3144
Size: 636 199 273 964 465 29 578
Fragments arranged by size:
964 636 578 465 273 199 29
EagI C'GGCC_G
Cuts at: 0 1108 3144
Size: 1108 2036
EarI CTCTTCn'nnn_
Cuts at: 0 202 271 284 3144
Size: 202 69 13 2860
Fragments arranged by size:
2860 202 69 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 903 3144
Size: 903 2241
EcoICRI GAG'CTC
Cuts at: 0 938 2751 3144
Size: 938 1813 393
Fragments arranged by size:
1813 938 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 396 470 937 1013 1186 1735 1977
Size: 65 331 74 467 76 173 549 242
Cuts at: 1977 2647 2744 2818 3065 3144
Size: 670 97 74 247 79
Fragments arranged by size:
670 549 467 331 247 242 173 97
79 76 74 74 65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3009 3144
Size: 3009 135
EcoO109I rG'GnC_Cy
Cuts at: 0 75 765 1038 1161 1524 1700 2144 3144
Size: 75 690 273 123 363 176 444 1000
Fragments arranged by size:
1000 690 444 363 273 176 123 75
HaeII r_GCGC'y
Cuts at: 0 144 240 1179 3119 3144
Size: 144 96 939 1940 25
Fragments arranged by size:
1940 939 144 96 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 464 496 1303 1335 3144
Size: 464 32 807 32 1809
Fragments arranged by size:
1809 807 464 32 32
KasI G'GCGC_C
Cuts at: 0 3115 3144
Size: 3115 29
KpnI G_GTAC'C
Cuts at: 0 985 3144
Size: 985 2159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2087 2189 2645 3144
Size: 2087 102 456 499
Fragments arranged by size:
2087 499 456 102
MslI CAynn'nnrTG
Cuts at: 0 524 1798 2495 3144
Size: 524 1274 697 649
Fragments arranged by size:
1274 697 649 524
MspA1I CmG'CkG
Cuts at: 0 721 1215 2952 3144
Size: 721 494 1737 192
Fragments arranged by size:
1737 721 494 192
NaeI GCC'GGC
Cuts at: 0 835 1133 3144
Size: 835 298 2011
Fragments arranged by size:
2011 835 298
NarI GG'CG_CC
Cuts at: 0 3116 3144
Size: 3116 28
NcoI C'CATG_G
Cuts at: 0 455 3144
Size: 455 2689
NgoMIV G'CCGG_C
Cuts at: 0 833 1131 3144
Size: 833 298 2013
Fragments arranged by size:
2013 833 298
NspI r_CATG'y
Cuts at: 0 391 2665 3024 3144
Size: 391 2274 359 120
Fragments arranged by size:
2274 391 359 120
PasI CC'CwG_GG
Cuts at: 0 1157 3144
Size: 1157 1987
PflMI CCAn_nnn'nTGG
Cuts at: 0 63 456 784 812 1265 1713 3144
Size: 63 393 328 28 453 448 1431
Fragments arranged by size:
1431 453 448 393 328 63 28
PfoI T'CCnGG_A
Cuts at: 0 1547 1964 3144
Size: 1547 417 1180
Fragments arranged by size:
1547 1180 417
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 1984 2016 3144
Size: 1107 32 845 32 1128
Fragments arranged by size:
1128 1107 845 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 75 1038 1524 1700 2144 3144
Size: 75 963 486 176 444 1000
Fragments arranged by size:
1000 963 486 444 176 75
PshAI GACnn'nnGTC
Cuts at: 0 2558 3144
Size: 2558 586
PspOMI G'GGCC_C
Cuts at: 0 765 1160 3144
Size: 765 395 1984
Fragments arranged by size:
1984 765 395
PspXI vC'TCGA_Gb
Cuts at: 0 470 620 1858 3144
Size: 470 150 1238 1286
Fragments arranged by size:
1286 1238 470 150
PstI C_TGCA'G
Cuts at: 0 1349 1778 2531 2957 3144
Size: 1349 429 753 426 187
Fragments arranged by size:
1349 753 429 426 187
PvuII CAG'CTG
Cuts at: 0 721 1215 2952 3144
Size: 721 494 1737 192
Fragments arranged by size:
1737 721 494 192
SacI G_AGCT'C
Cuts at: 0 940 2753 3144
Size: 940 1813 391
Fragments arranged by size:
1813 940 391
SanDI GG'GwC_CC
Cuts at: 0 1038 1700 2144 3144
Size: 1038 662 444 1000
Fragments arranged by size:
1038 1000 662 444
SapI GCTCTTCn'nnn_
Cuts at: 0 202 3144
Size: 202 2942
ScaI AGT'ACT
Cuts at: 0 1048 1090 3144
Size: 1048 42 2054
Fragments arranged by size:
2054 1048 42
SfcI C'TryA_G
Cuts at: 0 1345 1392 1774 2055 2527 2953 3144
Size: 1345 47 382 281 472 426 191
Fragments arranged by size:
1345 472 426 382 281 191 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 672 2545 3144
Size: 672 1873 599
Fragments arranged by size:
1873 672 599
SfoI GGC'GCC
Cuts at: 0 3117 3144
Size: 3117 27
SgrAI Cr'CCGG_yG
Cuts at: 0 884 3144
Size: 884 2260
SmaI CCC'GGG
Cuts at: 0 2005 2680 3144
Size: 2005 675 464
Fragments arranged by size:
2005 675 464
SmlI C'TyrA_G
Cuts at: 0 470 620 1858 3144
Size: 470 150 1238 1286
Fragments arranged by size:
1286 1238 470 150
SphI G_CATG'C
Cuts at: 0 391 2665 3024 3144
Size: 391 2274 359 120
Fragments arranged by size:
2274 391 359 120
StuI AGG'CCT
Cuts at: 0 442 666 3004 3144
Size: 442 224 2338 140
Fragments arranged by size:
2338 442 224 140
StyI C'CwwG_G
Cuts at: 0 182 455 1520 2174 3144
Size: 182 273 1065 654 970
Fragments arranged by size:
1065 970 654 273 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 570 951 1392 1443 1930 2492 3013 3144
Size: 570 381 441 51 487 562 521 131
Fragments arranged by size:
570 562 521 487 441 381 131 51
TatI w'GTAC_w
Cuts at: 0 474 1046 1088 3144
Size: 474 572 42 2056
Fragments arranged by size:
2056 572 474 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2364 2396 3144
Size: 2364 32 748
Fragments arranged by size:
2364 748 32
Tth111I GACn'n_nGTC
Cuts at: 0 2767 3144
Size: 2767 377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 3144
Size: 60 3084
XhoI C'TCGA_G
Cuts at: 0 470 620 1858 3144
Size: 470 150 1238 1286
Fragments arranged by size:
1286 1238 470 150
XmaI C'CCGG_G
Cuts at: 0 2003 2678 3144
Size: 2003 675 466
Fragments arranged by size:
2003 675 466
XmnI GAAnn'nnTTC
Cuts at: 0 998 3144
Size: 998 2146
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AloI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
KpnI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SanDI SapI ScaI SfcI
SfiI SfoI SgrAI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BclI BfrBI
BmtI BpuEI BsaAI BsiWI BspEI BspHI BspMI BsrGI
BssHII BstAPI BstBI BstZ17I ClaI DraI DrdI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI MscI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PsiI PsrI PvuI RsrII
SacII SalI SbfI SexAI SgfI SnaBI SpeI SrfI
SspI SwaI XbaI ZraI