Restriction map for
pF1KSDA0967
(Linear) (Six-base) MAPSORT of: KIAA0967.seq Check: 3622 from: 1 to: 4734
KIAA0967.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 716 726 1732 1864 4734
Size: 716 10 1006 132 2870
Fragments arranged by size:
2870 1006 716 132 10
AatII G_ACGT'C
Cuts at: 0 1397 4734
Size: 1397 3337
AccI GT'mk_AC
Cuts at: 0 2588 4734
Size: 2588 2146
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 475 506 762 1372 3932 4734
Size: 344 131 31 256 610 2560 802
Fragments arranged by size:
2560 802 610 344 256 131 31
AfeI AGC'GCT
Cuts at: 0 883 4734
Size: 883 3851
AflIII A'CryG_T
Cuts at: 0 2594 4734
Size: 2594 2140
AleI CACnn'nnGTG
Cuts at: 0 604 4734
Size: 604 4130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 886 4734
Size: 886 3848
AlwNI CAG_nnn'CTG
Cuts at: 0 785 1565 1724 2941 3074 4488 4734
Size: 785 780 159 1217 133 1414 246
Fragments arranged by size:
1414 1217 785 780 246 159 133
ApaI G_GGCC'C
Cuts at: 0 128 1717 2360 4734
Size: 128 1589 643 2374
Fragments arranged by size:
2374 1589 643 128
ApaLI G'TGCA_C
Cuts at: 0 475 2633 4350 4671 4734
Size: 475 2158 1717 321 63
Fragments arranged by size:
2158 1717 475 321 63
ApoI r'AATT_y
Cuts at: 0 1098 2862 4734
Size: 1098 1764 1872
Fragments arranged by size:
1872 1764 1098
AvaI C'yCGr_G
Cuts at: 0 83 98 1812 2773 2807 3408 4148 4734
Size: 83 15 1714 961 34 601 740 586
Fragments arranged by size:
1714 961 740 601 586 83 34 15
AvrII C'CTAG_G
Cuts at: 0 3721 4734
Size: 3721 1013
BamHI G'GATC_C
Cuts at: 0 3795 4734
Size: 3795 939
BanI G'GyrC_C
Cuts at: 0 1625 1641 2055 2562 3361 3687 4734
Size: 1625 16 414 507 799 326 1047
Fragments arranged by size:
1625 1047 799 507 414 326 16
BanII G_rGCy'C
Cuts at: 0 128 1717 2199 2360 2618 2774 3131 3143
Size: 128 1589 482 161 258 156 357 12
Cuts at: 3143 4155 4396 4734
Size: 1012 241 338
Fragments arranged by size:
1589 1012 482 357 338 258 241 161
156 128 12
BbeI G_GCGC'C
Cuts at: 0 1629 4734
Size: 1629 3105
BbsI GAAGACnn'nnnn_
Cuts at: 0 332 463 830 2017 2463 2807 4067 4734
Size: 332 131 367 1187 446 344 1260 667
Fragments arranged by size:
1260 1187 667 446 367 344 332 131
BbvCI CC'TCA_GC
Cuts at: 0 2892 4734
Size: 2892 1842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 646 680 2014 2048 4160 4194 4734
Size: 646 34 1334 34 2112 34 540
Fragments arranged by size:
2112 1334 646 540 34 34 34
BclI T'GATC_A
Cuts at: 0 291 4427 4734
Size: 291 4136 307
Fragments arranged by size:
4136 307 291
BglI GCCn_nnn'nGGC
Cuts at: 0 2445 3079 4140 4684 4734
Size: 2445 634 1061 544 50
Fragments arranged by size:
2445 1061 634 544 50
BglII A'GATC_T
Cuts at: 0 974 4734
Size: 974 3760
Bme1580I G_kGCm'C
Cuts at: 0 128 479 963 1717 2058 2360 2637 4221
Size: 128 351 484 754 341 302 277 1584
Cuts at: 4221 4354 4675 4734
Size: 133 321 59
Fragments arranged by size:
1584 754 484 351 341 321 302 277
133 128 59
BmgBI CAC'GTC
Cuts at: 0 404 2595 4734
Size: 404 2191 2139
Fragments arranged by size:
2191 2139 404
BmrI ACTGGGnnnn_n'
Cuts at: 0 1011 1477 1995 2234 2378 4734
Size: 1011 466 518 239 144 2356
Fragments arranged by size:
2356 1011 518 466 239 144
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1602 4227 4734
Size: 1602 2625 507
Fragments arranged by size:
2625 1602 507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 181 859 1848 2599 2710 2736 3030
Size: 31 150 678 989 751 111 26 294
Cuts at: 3030 3115 3440 3650 4158 4197 4309 4734
Size: 85 325 210 508 39 112 425
Fragments arranged by size:
989 751 678 508 425 325 294 210
150 112 111 85 39 31 26
Bpu10I CC'TnA_GC
Cuts at: 0 2892 2945 3755 4734
Size: 2892 53 810 979
Fragments arranged by size:
2892 979 810 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1162 2963 3095 3527 3569 4734
Size: 532 630 1801 132 432 42 1165
Fragments arranged by size:
1801 1165 630 532 432 132 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 2553 2686 2722 2770 3530 4734
Size: 7 2546 133 36 48 760 1204
Fragments arranged by size:
2546 1204 760 133 48 36 7
BsaBI GATnn'nnATC
Cuts at: 0 3168 4734
Size: 3168 1566
BsaHI Gr'CG_yC
Cuts at: 0 1394 1626 1765 4734
Size: 1394 232 139 2969
Fragments arranged by size:
2969 1394 232 139
BsaWI w'CCGG_w
Cuts at: 0 2837 4734
Size: 2837 1897
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 625 655 679 709 1843 1873 2734 2764
Size: 625 30 24 30 1134 30 861 30
Cuts at: 2764 3113 3143 4734
Size: 349 30 1591
Fragments arranged by size:
1591 1134 861 625 349 30 30 30
30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 451 766 1594 1681 1851 1948 2708
Size: 184 267 315 828 87 170 97 760
Cuts at: 2708 2880 2894 3394 4734
Size: 172 14 500 1340
Fragments arranged by size:
1340 828 760 500 315 267 184 172
170 97 87 14
BseYI C'CCAG_C
Cuts at: 0 962 1139 1456 1717 2051 2234 2745 4734
Size: 962 177 317 261 334 183 511 1989
Fragments arranged by size:
1989 962 511 334 317 261 183 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 688 697 896 916 2251 4020 4146
Size: 384 304 9 199 20 1335 1769 126
Cuts at: 4146 4734
Size: 588
Fragments arranged by size:
1769 1335 588 384 304 199 126 20
9
BsiEI CG_ry'CG
Cuts at: 0 4688 4734
Size: 4688 46
BsiHKAI G_wGCw'C
Cuts at: 0 479 497 548 875 2071 2637 2740 3131
Size: 479 18 51 327 1196 566 103 391
Cuts at: 3131 4354 4675 4734
Size: 1223 321 59
Fragments arranged by size:
1223 1196 566 479 391 327 321 103
59 51 18
Bsp1286I G_dGCh'C
Cuts at: 0 128 479 497 548 875 963 1717 2058
Size: 128 351 18 51 327 88 754 341
Cuts at: 2058 2071 2199 2360 2618 2637 2740 2774 3131
Size: 13 128 161 258 19 103 34 357
Cuts at: 3131 3143 4155 4221 4354 4396 4675 4734
Size: 12 1012 66 133 42 279 59
Fragments arranged by size:
1012 754 357 351 341 327 279 258
161 133 128 128 103 88 66 59
51 42 34 19 18 13 12
BspEI T'CCGG_A
Cuts at: 0 2837 4734
Size: 2837 1897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 716 726 1732 1859 1864 4630 4734
Size: 716 10 1006 127 5 2766 104
Fragments arranged by size:
2766 1006 716 127 104 10 5
BsrDI GCAATG_nn'
Cuts at: 0 1238 3819 4734
Size: 1238 2581 915
Fragments arranged by size:
2581 1238 915
BsrFI r'CCGG_y
Cuts at: 0 4501 4734
Size: 4501 233
BsrGI T'GTAC_A
Cuts at: 0 3178 4583 4734
Size: 3178 1405 151
Fragments arranged by size:
3178 1405 151
BstAPI GCAn_nnn'nTGC
Cuts at: 0 711 1321 2667 4734
Size: 711 610 1346 2067
Fragments arranged by size:
2067 1346 711 610
BstEII G'GTnAC_C
Cuts at: 0 3052 4734
Size: 3052 1682
BstXI CCAn_nnnn'nTGG
Cuts at: 0 205 2703 2760 3945 4734
Size: 205 2498 57 1185 789
Fragments arranged by size:
2498 1185 789 205 57
BstYI r'GATC_y
Cuts at: 0 296 947 974 1163 3515 3795 4734
Size: 296 651 27 189 2352 280 939
Fragments arranged by size:
2352 939 651 296 280 189 27
Bsu36I CC'TnA_GG
Cuts at: 0 1697 2503 4256 4570 4734
Size: 1697 806 1753 314 164
Fragments arranged by size:
1753 1697 806 314 164
BtgI C'CryG_G
Cuts at: 0 830 1791 1823 2780 4032 4085 4724 4734
Size: 830 961 32 957 1252 53 639 10
Fragments arranged by size:
1252 961 957 830 639 53 32 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 881 4734
Size: 881 3853
BtsI GCAGTG_nn'
Cuts at: 0 1575 4734
Size: 1575 3159
DraI TTT'AAA
Cuts at: 0 983 1151 4734
Size: 983 168 3583
Fragments arranged by size:
3583 983 168
EaeI y'GGCC_r
Cuts at: 0 2144 3080 3935 4198 4499 4685 4734
Size: 2144 936 855 263 301 186 49
Fragments arranged by size:
2144 936 855 301 263 186 49
EagI C'GGCC_G
Cuts at: 0 4685 4734
Size: 4685 49
EarI CTCTTCn'nnn_
Cuts at: 0 436 674 713 781 1081 1346 1832 3299
Size: 436 238 39 68 300 265 486 1467
Cuts at: 3299 4445 4734
Size: 1146 289
Fragments arranged by size:
1467 1146 486 436 300 289 265 238
68 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4530 4734
Size: 4530 204
EcoICRI GAG'CTC
Cuts at: 0 3129 4734
Size: 3129 1605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 181 344 475 506 762 859 1372
Size: 31 150 163 131 31 256 97 513
Cuts at: 1372 1848 2599 2710 2736 3030 3115 3440 3650
Size: 476 751 111 26 294 85 325 210
Cuts at: 3650 3932 4158 4197 4309 4734
Size: 282 226 39 112 425
Fragments arranged by size:
751 513 476 425 325 294 282 256
226 210 163 150 131 112 111 97
85 39 31 31 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 196 433 1866 3500 4734
Size: 196 237 1433 1634 1234
Fragments arranged by size:
1634 1433 1234 237 196
EcoO109I rG'GnC_Cy
Cuts at: 0 801 1562 1713 1714 2344 2356 4734
Size: 801 761 151 1 630 12 2378
Fragments arranged by size:
2378 801 761 630 151 12 1
EcoRI G'AATT_C
Cuts at: 0 2862 4734
Size: 2862 1872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 394 495 573 1402 2534 4734
Size: 394 101 78 829 1132 2200
Fragments arranged by size:
2200 1132 829 394 101 78
FseI GG_CCGG'CC
Cuts at: 0 4505 4734
Size: 4505 229
HaeII r_GCGC'y
Cuts at: 0 81 885 1629 4734
Size: 81 804 744 3105
Fragments arranged by size:
3105 804 744 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 625 657 1216 1248 1843 1875 2281 2313
Size: 625 32 559 32 595 32 406 32
Cuts at: 2313 2524 2556 3436 3468 3960 3992 4734
Size: 211 32 880 32 492 32 742
Fragments arranged by size:
880 742 625 595 559 492 406 211
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1479 4734
Size: 1479 3255
HindIII A'AGCT_T
Cuts at: 0 277 578 2394 4734
Size: 277 301 1816 2340
Fragments arranged by size:
2340 1816 301 277
KasI G'GCGC_C
Cuts at: 0 1625 4734
Size: 1625 3109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 942 1051 1233 1448 1793 3116 3134 3590
Size: 942 109 182 215 345 1323 18 456
Cuts at: 3590 4293 4305 4734
Size: 703 12 429
Fragments arranged by size:
1323 942 703 456 429 345 215 182
109 18 12
MscI TGG'CCA
Cuts at: 0 3082 3937 4200 4734
Size: 3082 855 263 534
Fragments arranged by size:
3082 855 534 263
MslI CAynn'nnrTG
Cuts at: 0 157 604 2764 3780 4734
Size: 157 447 2160 1016 954
Fragments arranged by size:
2160 1016 954 447 157
MspA1I CmG'CkG
Cuts at: 0 118 770 1793 3460 4004 4160 4230 4497
Size: 118 652 1023 1667 544 156 70 267
Cuts at: 4497 4535 4561 4734
Size: 38 26 173
Fragments arranged by size:
1667 1023 652 544 267 173 156 118
70 38 26
NaeI GCC'GGC
Cuts at: 0 4503 4734
Size: 4503 231
NarI GG'CG_CC
Cuts at: 0 1626 4734
Size: 1626 3108
NcoI C'CATG_G
Cuts at: 0 1823 2780 4085 4734
Size: 1823 957 1305 649
Fragments arranged by size:
1823 1305 957 649
NdeI CA'TA_TG
Cuts at: 0 1185 4734
Size: 1185 3549
NgoMIV G'CCGG_C
Cuts at: 0 4501 4734
Size: 4501 233
NspI r_CATG'y
Cuts at: 0 2670 4028 4426 4734
Size: 2670 1358 398 308
Fragments arranged by size:
2670 1358 398 308
PasI CC'CwG_GG
Cuts at: 0 3565 4734
Size: 3565 1169
PflMI CCAn_nnn'nTGG
Cuts at: 0 738 4275 4488 4734
Size: 738 3537 213 246
Fragments arranged by size:
3537 738 246 213
PfoI T'CCnGG_A
Cuts at: 0 197 875 938 1973 3270 4734
Size: 197 678 63 1035 1297 1464
Fragments arranged by size:
1464 1297 1035 678 197 63
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2148 2180 4734
Size: 2148 32 2554
Fragments arranged by size:
2554 2148 32
PpuMI rG'GwC_Cy
Cuts at: 0 801 4734
Size: 801 3933
PspOMI G'GGCC_C
Cuts at: 0 124 1713 2356 4734
Size: 124 1589 643 2378
Fragments arranged by size:
2378 1589 643 124
PspXI vC'TCGA_Gb
Cuts at: 0 1812 4734
Size: 1812 2922
PstI C_TGCA'G
Cuts at: 0 117 857 865 1542 1570 1651 2379 2663
Size: 117 740 8 677 28 81 728 284
Cuts at: 2663 4734
Size: 2071
Fragments arranged by size:
2071 740 728 677 284 117 81 28
8
PvuII CAG'CTG
Cuts at: 0 118 3460 4004 4160 4230 4497 4734
Size: 118 3342 544 156 70 267 237
Fragments arranged by size:
3342 544 267 237 156 118 70
SacI G_AGCT'C
Cuts at: 0 3131 4734
Size: 3131 1603
SacII CC_GC'GG
Cuts at: 0 1794 4734
Size: 1794 2940
SapI GCTCTTCn'nnn_
Cuts at: 0 674 3299 4734
Size: 674 2625 1435
Fragments arranged by size:
2625 1435 674
ScaI AGT'ACT
Cuts at: 0 661 4207 4734
Size: 661 3546 527
Fragments arranged by size:
3546 661 527
SexAI A'CCwGG_T
Cuts at: 0 4487 4734
Size: 4487 247
SfcI C'TryA_G
Cuts at: 0 113 853 861 890 1159 1464 1538 1566
Size: 113 740 8 29 269 305 74 28
Cuts at: 1566 1647 1797 2118 2375 2524 2659 4527 4734
Size: 81 150 321 257 149 135 1868 207
Fragments arranged by size:
1868 740 321 305 269 257 207 150
149 135 113 81 74 29 28 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4684 4734
Size: 4684 50
SfoI GGC'GCC
Cuts at: 0 1627 4734
Size: 1627 3107
SmaI CCC'GGG
Cuts at: 0 85 100 3410 4734
Size: 85 15 3310 1324
Fragments arranged by size:
3310 1324 85 15
SmlI C'TyrA_G
Cuts at: 0 547 1177 1812 2807 2978 3110 3542 3584
Size: 547 630 635 995 171 132 432 42
Cuts at: 3584 4734
Size: 1150
Fragments arranged by size:
1150 995 635 630 547 432 171 132
42
SrfI GCCC'GGGC
Cuts at: 0 100 4734
Size: 100 4634
StuI AGG'CCT
Cuts at: 0 1901 2701 2955 4143 4734
Size: 1901 800 254 1188 591
Fragments arranged by size:
1901 1188 800 591 254
StyI C'CwwG_G
Cuts at: 0 797 1823 2628 2696 2702 2780 3721 4085
Size: 797 1026 805 68 6 78 941 364
Cuts at: 4085 4734
Size: 649
Fragments arranged by size:
1026 941 805 797 649 364 78 68
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 448 2285 2545 3523 4734
Size: 448 1837 260 978 1211
Fragments arranged by size:
1837 1211 978 448 260
TatI w'GTAC_w
Cuts at: 0 659 686 2173 3178 4205 4583 4734
Size: 659 27 1487 1005 1027 378 151
Fragments arranged by size:
1487 1027 1005 659 378 151 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3113 3145 3644 3676 4109 4141 4734
Size: 3113 32 499 32 433 32 593
Fragments arranged by size:
3113 593 499 433 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 804 1698 1830 2247 3930 3987 4734
Size: 804 894 132 417 1683 57 747
Fragments arranged by size:
1683 894 804 747 417 132 57
XhoI C'TCGA_G
Cuts at: 0 1812 2807 4734
Size: 1812 995 1927
Fragments arranged by size:
1927 1812 995
XmaI C'CCGG_G
Cuts at: 0 83 98 3408 4734
Size: 83 15 3310 1326
Fragments arranged by size:
3310 1326 83 15
ZraI GAC'GTC
Cuts at: 0 1395 4734
Size: 1395 3339
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SacII SapI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AloI AscI AseI
AsiSI BaeI BciVI BfrBI BlpI BmtI BsaAI BsiWI
BsmI BsmBI BspHI BsrBI BssHII BssSI BstBI BstZ17I
ClaI CspCI DraIII DrdI EcoRV FspI FspAI HpaI
KpnI MfeI MluI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PshAI PsiI PsrI PvuI RsrII
SalI SanDI SbfI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XmnI