Restriction map for
pF1KA0966
(Linear) (Six-base) MAPSORT of: KIAA0966.seq Check: 8874 from: 1 to: 3396
KIAA0966.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 1133 3396
Size: 1133 2263
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 520 1195 1585 2152 2165 2540 2738
Size: 412 108 675 390 567 13 375 198
Cuts at: 2738 3161 3396
Size: 423 235
Fragments arranged by size:
675 567 423 412 390 375 235 198
108 13
AflIII A'CryG_T
Cuts at: 0 2549 3396
Size: 2549 847
AleI CACnn'nnGTG
Cuts at: 0 1396 2512 3396
Size: 1396 1116 884
Fragments arranged by size:
1396 1116 884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2095 3116 3396
Size: 2095 1021 280
Fragments arranged by size:
2095 1021 280
AlwNI CAG_nnn'CTG
Cuts at: 0 1440 2539 3319 3396
Size: 1440 1099 780 77
Fragments arranged by size:
1440 1099 780 77
ApaI G_GGCC'C
Cuts at: 0 1866 3396
Size: 1866 1530
ApoI r'AATT_y
Cuts at: 0 161 2322 2338 2584 2595 3396
Size: 161 2161 16 246 11 801
Fragments arranged by size:
2161 801 246 161 16 11
AseI AT'TA_AT
Cuts at: 0 3329 3396
Size: 3329 67
BanII G_rGCy'C
Cuts at: 0 8 317 1866 3396
Size: 8 309 1549 1530
Fragments arranged by size:
1549 1530 309 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 400 2276 2546 2618 3396
Size: 400 1876 270 72 778
Fragments arranged by size:
1876 778 400 270 72
BbvCI CC'TCA_GC
Cuts at: 0 2688 3396
Size: 2688 708
BglI GCCn_nnn'nGGC
Cuts at: 0 74 603 3396
Size: 74 529 2793
Fragments arranged by size:
2793 529 74
BglII A'GATC_T
Cuts at: 0 177 651 1716 2932 3396
Size: 177 474 1065 1216 464
Fragments arranged by size:
1216 1065 474 464 177
Bme1580I G_kGCm'C
Cuts at: 0 1080 1866 3396
Size: 1080 786 1530
Fragments arranged by size:
1530 1080 786
BmgBI CAC'GTC
Cuts at: 0 1911 3396
Size: 1911 1485
BmrI ACTGGGnnnn_n'
Cuts at: 0 587 2709 3396
Size: 587 2122 687
Fragments arranged by size:
2122 687 587
BmtI G_CTAG'C
Cuts at: 0 2626 3396
Size: 2626 770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 656 3296 3396
Size: 67 589 2640 100
Fragments arranged by size:
2640 589 100 67
Bpu10I CC'TnA_GC
Cuts at: 0 2688 3396
Size: 2688 708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 2263 3166 3396
Size: 325 1938 903 230
Fragments arranged by size:
1938 903 325 230
BsaI GGTCTCn'nnnn_
Cuts at: 0 230 772 2294 2920 3396
Size: 230 542 1522 626 476
Fragments arranged by size:
1522 626 542 476 230
BsaAI yAC'GTr
Cuts at: 0 1950 3396
Size: 1950 1446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3156 3186 3396
Size: 3156 30 210
Fragments arranged by size:
3156 210 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 492 902 3396
Size: 492 410 2494
Fragments arranged by size:
2494 492 410
BseYI C'CCAG_C
Cuts at: 0 1330 1549 1859 3396
Size: 1330 219 310 1537
Fragments arranged by size:
1537 1330 310 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 583 736 2065 3396
Size: 81 502 153 1329 1331
Fragments arranged by size:
1331 1329 502 153 81
BsiEI CG_ry'CG
Cuts at: 0 594 3396
Size: 594 2802
BsiHKAI G_wGCw'C
Cuts at: 0 8 275 317 577 1238 3396
Size: 8 267 42 260 661 2158
Fragments arranged by size:
2158 661 267 260 42 8
BsmI GAATG_Cn'
Cuts at: 0 875 1700 3093 3396
Size: 875 825 1393 303
Fragments arranged by size:
1393 875 825 303
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1901 3396
Size: 1901 1495
Bsp1286I G_dGCh'C
Cuts at: 0 8 275 317 577 1080 1238 1866 3396
Size: 8 267 42 260 503 158 628 1530
Fragments arranged by size:
1530 628 503 267 260 158 42 8
BsrDI GCAATG_nn'
Cuts at: 0 2119 2174 3396
Size: 2119 55 1222
Fragments arranged by size:
2119 1222 55
BssHII G'CGCG_C
Cuts at: 0 48 50 3396
Size: 48 2 3346
Fragments arranged by size:
3346 48 2
BssSI C'ACGA_G
Cuts at: 0 977 3396
Size: 977 2419
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1185 3396
Size: 1185 2211
BstBI TT'CG_AA
Cuts at: 0 1085 3222 3396
Size: 1085 2137 174
Fragments arranged by size:
2137 1085 174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 921 2865 3396
Size: 132 789 1944 531
Fragments arranged by size:
1944 789 531 132
BstYI r'GATC_y
Cuts at: 0 177 300 651 1716 2932 3396
Size: 177 123 351 1065 1216 464
Fragments arranged by size:
1216 1065 464 351 177 123
Bsu36I CC'TnA_GG
Cuts at: 0 296 788 3396
Size: 296 492 2608
Fragments arranged by size:
2608 492 296
BtgI C'CryG_G
Cuts at: 0 184 1033 1851 2207 3230 3270 3396
Size: 184 849 818 356 1023 40 126
Fragments arranged by size:
1023 849 818 356 184 126 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1178 1398 3017 3396
Size: 1178 220 1619 379
Fragments arranged by size:
1619 1178 379 220
BtsI GCAGTG_nn'
Cuts at: 0 1236 3293 3396
Size: 1236 2057 103
Fragments arranged by size:
2057 1236 103
ClaI AT'CG_AT
Cuts at: 0 1017 3396
Size: 1017 2379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2031 2066 3056 3091 3396
Size: 2031 35 990 35 305
Fragments arranged by size:
2031 990 305 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 244 3396
Size: 244 3152
EaeI y'GGCC_r
Cuts at: 0 3028 3396
Size: 3028 368
EarI CTCTTCn'nnn_
Cuts at: 0 13 485 879 1140 2141 2245 3298 3396
Size: 13 472 394 261 1001 104 1053 98
Fragments arranged by size:
1053 1001 472 394 261 104 98 13
EcoICRI GAG'CTC
Cuts at: 0 6 315 3396
Size: 6 309 3081
Fragments arranged by size:
3081 309 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 412 520 656 1195 1585 2152 2165
Size: 67 345 108 136 539 390 567 13
Cuts at: 2165 2540 2738 3161 3296 3396
Size: 375 198 423 135 100
Fragments arranged by size:
567 539 423 390 375 345 198 136
135 108 100 67 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 3396
Size: 37 3359
EcoO109I rG'GnC_Cy
Cuts at: 0 1863 2032 3396
Size: 1863 169 1364
Fragments arranged by size:
1863 1364 169
EcoRI G'AATT_C
Cuts at: 0 2584 3396
Size: 2584 812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1204 1590 2182 2485 3396
Size: 1204 386 592 303 911
Fragments arranged by size:
1204 911 592 386 303
HaeII r_GCGC'y
Cuts at: 0 2695 3396
Size: 2695 701
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 576 608 636 654 668 686
Size: 505 32 39 32 28 18 14 18
Cuts at: 686 771 803 1545 1577 1631 1663 1701 1733
Size: 85 32 742 32 54 32 38 32
Cuts at: 1733 2551 2583 2917 2949 3009 3041 3396
Size: 818 32 334 32 60 32 355
Fragments arranged by size:
818 742 505 355 334 85 60 54
39 38 32 32 32 32 32 32
32 32 32 28 18 18 14
HincII GTy'rAC
Cuts at: 0 2439 3396
Size: 2439 957
HindIII A'AGCT_T
Cuts at: 0 1754 3396
Size: 1754 1642
HpaI GTT'AAC
Cuts at: 0 2439 3396
Size: 2439 957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 452 3396
Size: 114 338 2944
Fragments arranged by size:
2944 338 114
MslI CAynn'nnrTG
Cuts at: 0 1396 1705 2512 2579 2777 3396
Size: 1396 309 807 67 198 619
Fragments arranged by size:
1396 807 619 309 198 67
MspA1I CmG'CkG
Cuts at: 0 1035 1440 1558 3396
Size: 1035 405 118 1838
Fragments arranged by size:
1838 1035 405 118
NcoI C'CATG_G
Cuts at: 0 184 1851 2207 3230 3270 3396
Size: 184 1667 356 1023 40 126
Fragments arranged by size:
1667 1023 356 184 126 40
NheI G'CTAG_C
Cuts at: 0 2622 3396
Size: 2622 774
NruI TCG'CGA
Cuts at: 0 1417 3396
Size: 1417 1979
NspI r_CATG'y
Cuts at: 0 2076 2553 3138 3396
Size: 2076 477 585 258
Fragments arranged by size:
2076 585 477 258
PasI CC'CwG_GG
Cuts at: 0 2313 3396
Size: 2313 1083
PciI A'CATG_T
Cuts at: 0 2549 3396
Size: 2549 847
PspOMI G'GGCC_C
Cuts at: 0 1862 3396
Size: 1862 1534
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1460 1492 3396
Size: 1460 32 1904
Fragments arranged by size:
1904 1460 32
PstI C_TGCA'G
Cuts at: 0 38 2192 3396
Size: 38 2154 1204
Fragments arranged by size:
2154 1204 38
PvuII CAG'CTG
Cuts at: 0 1440 1558 3396
Size: 1440 118 1838
Fragments arranged by size:
1838 1440 118
SacI G_AGCT'C
Cuts at: 0 8 317 3396
Size: 8 309 3079
Fragments arranged by size:
3079 309 8
SacII CC_GC'GG
Cuts at: 0 1036 3396
Size: 1036 2360
SapI GCTCTTCn'nnn_
Cuts at: 0 13 2245 3396
Size: 13 2232 1151
Fragments arranged by size:
2232 1151 13
SfcI C'TryA_G
Cuts at: 0 34 2085 2188 3396
Size: 34 2051 103 1208
Fragments arranged by size:
2051 1208 103 34
SmlI C'TyrA_G
Cuts at: 0 304 2242 3145 3396
Size: 304 1938 903 251
Fragments arranged by size:
1938 903 304 251
SphI G_CATG'C
Cuts at: 0 2076 3138 3396
Size: 2076 1062 258
Fragments arranged by size:
2076 1062 258
SspI AAT'ATT
Cuts at: 0 2388 3396
Size: 2388 1008
StyI C'CwwG_G
Cuts at: 0 17 184 705 1736 1851 2207 2298 3230
Size: 17 167 521 1031 115 356 91 932
Cuts at: 3230 3270 3396
Size: 40 126
Fragments arranged by size:
1031 932 521 356 167 126 115 91
40 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 635 833 2699 3396
Size: 635 198 1866 697
Fragments arranged by size:
1866 697 635 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1347 1379 3396
Size: 1347 32 2017
Fragments arranged by size:
2017 1347 32
Tth111I GACn'n_nGTC
Cuts at: 0 2000 3396
Size: 2000 1396
XbaI T'CTAG_A
Cuts at: 0 2004 3396
Size: 2004 1392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1419 2205 3396
Size: 1419 786 1191
Fragments arranged by size:
1419 1191 786
XmnI GAAnn'nnTTC
Cuts at: 0 505 3396
Size: 505 2891
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI ApoI
AseI BanII BbsI BbvCI BglI BglII Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BsrDI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII HpaI MmeI MslI MspA1I NcoI NheI
NruI NspI PasI PciI PspOMI PsrI PstI PvuII
SacI SacII SapI SfcI SmlI SphI SspI StyI
TaqII TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BclI BfrBI BlpI BplI
BsaBI BsaHI BsaWI BsiWI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BstEII BstZ17I DraI DraIII EagI EciI
EcoRV FseI FspI FspAI KasI KpnI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NotI NsiI PacI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI TatI XhoI XmaI ZraI