Restriction map for
pF1KSDA0963
(Linear) (Six-base) MAPSORT of: KIAA0963.seq Check: 6526 from: 1 to: 4098
KIAA0963.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 896 3060 3096 4098
Size: 896 2164 36 1002
Fragments arranged by size:
2164 1002 896 36
AatII G_ACGT'C
Cuts at: 0 3554 4098
Size: 3554 544
AccI GT'mk_AC
Cuts at: 0 1250 1505 4098
Size: 1250 255 2593
Fragments arranged by size:
2593 1250 255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1820 2161 3163 3607 4098
Size: 1820 341 1002 444 491
Fragments arranged by size:
1820 1002 491 444 341
AfeI AGC'GCT
Cuts at: 0 1624 2359 4098
Size: 1624 735 1739
Fragments arranged by size:
1739 1624 735
AflIII A'CryG_T
Cuts at: 0 1544 4098
Size: 1544 2554
AgeI A'CCGG_T
Cuts at: 0 2525 4098
Size: 2525 1573
AhdI GACnn_n'nnGTC
Cuts at: 0 339 4098
Size: 339 3759
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1003 1225 3586 4098
Size: 1003 222 2361 512
Fragments arranged by size:
2361 1003 512 222
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2575 2607 4098
Size: 2575 32 1491
Fragments arranged by size:
2575 1491 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1116 2151 2322 2823 3422 3474 3660 4098
Size: 1116 1035 171 501 599 52 186 438
Fragments arranged by size:
1116 1035 599 501 438 186 171 52
ApaI G_GGCC'C
Cuts at: 0 17 738 2634 2715 3187 4098
Size: 17 721 1896 81 472 911
Fragments arranged by size:
1896 911 721 472 81 17
ApaLI G'TGCA_C
Cuts at: 0 988 2461 2929 4098
Size: 988 1473 468 1169
Fragments arranged by size:
1473 1169 988 468
AvaI C'yCGr_G
Cuts at: 0 1679 1892 2011 2113 2409 2708 3085 3628
Size: 1679 213 119 102 296 299 377 543
Cuts at: 3628 4039 4098
Size: 411 59
Fragments arranged by size:
1679 543 411 377 299 296 213 119
102 59
BamHI G'GATC_C
Cuts at: 0 2077 4098
Size: 2077 2021
BanI G'GyrC_C
Cuts at: 0 1267 1381 1772 2453 2704 3089 3485 3696
Size: 1267 114 391 681 251 385 396 211
Cuts at: 3696 3831 3874 4081 4098
Size: 135 43 207 17
Fragments arranged by size:
1267 681 396 391 385 251 211 207
135 114 43 17
BanII G_rGCy'C
Cuts at: 0 17 181 738 2060 2210 2634 2715 3187
Size: 17 164 557 1322 150 424 81 472
Cuts at: 3187 3374 4098
Size: 187 724
Fragments arranged by size:
1322 724 557 472 424 187 164 150
81 17
BbeI G_GCGC'C
Cuts at: 0 1385 2457 3700 3835 4085 4098
Size: 1385 1072 1243 135 250 13
Fragments arranged by size:
1385 1243 1072 250 135 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 2550 3105 3595 4098
Size: 2550 555 490 503
Fragments arranged by size:
2550 555 503 490
BbvCI CC'TCA_GC
Cuts at: 0 563 2674 4098
Size: 563 2111 1424
Fragments arranged by size:
2111 1424 563
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 400 434 1081 1115 1410 1444 1685 1719
Size: 400 34 647 34 295 34 241 34
Cuts at: 1719 4098
Size: 2379
Fragments arranged by size:
2379 647 400 295 241 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2770 4098
Size: 2770 1328
BglI GCCn_nnn'nGGC
Cuts at: 0 232 2174 3544 4098
Size: 232 1942 1370 554
Fragments arranged by size:
1942 1370 554 232
BglII A'GATC_T
Cuts at: 0 3050 4098
Size: 3050 1048
BlpI GC'TnA_GC
Cuts at: 0 451 997 1441 4098
Size: 451 546 444 2657
Fragments arranged by size:
2657 546 451 444
Bme1580I G_kGCm'C
Cuts at: 0 17 593 738 992 1327 1775 2465 2634
Size: 17 576 145 254 335 448 690 169
Cuts at: 2634 2715 2771 2933 3092 3187 3877 4098
Size: 81 56 162 159 95 690 221
Fragments arranged by size:
690 690 576 448 335 254 221 169
162 159 145 95 81 56 17
BmgBI CAC'GTC
Cuts at: 0 2874 3681 4098
Size: 2874 807 417
Fragments arranged by size:
2874 807 417
BmrI ACTGGGnnnn_n'
Cuts at: 0 2057 4098
Size: 2057 2041
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 318 4098
Size: 318 3780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 228 898 1597 2143 2388 2638 2804
Size: 138 90 670 699 546 245 250 166
Cuts at: 2804 2944 3331 3967 4098
Size: 140 387 636 131
Fragments arranged by size:
699 670 636 546 387 250 245 166
140 138 131 90
Bpu10I CC'TnA_GC
Cuts at: 0 275 563 2674 2740 4098
Size: 275 288 2111 66 1358
Fragments arranged by size:
2111 1358 288 275 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 4098
Size: 919 3179
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 658 2093 2544 2639 4098
Size: 592 66 1435 451 95 1459
Fragments arranged by size:
1459 1435 592 451 95 66
BsaAI yAC'GTr
Cuts at: 0 615 2337 4098
Size: 615 1722 1761
Fragments arranged by size:
1761 1722 615
BsaHI Gr'CG_yC
Cuts at: 0 1037 1145 1382 1454 1940 2454 3155 3551
Size: 1037 108 237 72 486 514 701 396
Cuts at: 3551 3697 3832 3848 3860 3884 4082 4098
Size: 146 135 16 12 24 198 16
Fragments arranged by size:
1037 701 514 486 396 237 198 146
135 108 72 24 16 16 12
BsaWI w'CCGG_w
Cuts at: 0 1334 2525 4098
Size: 1334 1191 1573
Fragments arranged by size:
1573 1334 1191
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1987 2017 2467 2497 2524 2554 2575 2605
Size: 1987 30 450 30 27 30 21 30
Cuts at: 2605 4098
Size: 1493
Fragments arranged by size:
1987 1493 450 30 30 30 30 27
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 273 312 562 565 580 583 586 595
Size: 273 39 250 3 15 3 3 9
Cuts at: 595 1360 1486 1699 1877 1995 3061 3073 3373
Size: 765 126 213 178 118 1066 12 300
Cuts at: 3373 4082 4098
Size: 709 16
Fragments arranged by size:
1066 765 709 300 273 250 213 178
126 118 39 16 15 12 9 3
3 3
BseYI C'CCAG_C
Cuts at: 0 585 799 1488 1755 2172 2220 2231 3232
Size: 585 214 689 267 417 48 11 1001
Cuts at: 3232 3870 3996 4098
Size: 638 126 102
Fragments arranged by size:
1001 689 638 585 417 267 214 126
102 48 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 553 1339 2701 3952 4098
Size: 88 465 786 1362 1251 146
Fragments arranged by size:
1362 1251 786 465 146 88
BsiEI CG_ry'CG
Cuts at: 0 897 1562 1796 2073 2433 2496 2517 3297
Size: 897 665 234 277 360 63 21 780
Cuts at: 3297 3794 4098
Size: 497 304
Fragments arranged by size:
897 780 665 497 360 304 277 234
63 21
BsiHKAI G_wGCw'C
Cuts at: 0 181 992 2210 2465 2933 4098
Size: 181 811 1218 255 468 1165
Fragments arranged by size:
1218 1165 811 468 255 181
BsmI GAATG_Cn'
Cuts at: 0 1185 2946 4098
Size: 1185 1761 1152
Fragments arranged by size:
1761 1185 1152
BsmBI CGTCTCn'nnnn_
Cuts at: 0 927 1794 4098
Size: 927 867 2304
Fragments arranged by size:
2304 927 867
Bsp1286I G_dGCh'C
Cuts at: 0 17 181 593 738 992 1327 1775 2060
Size: 17 164 412 145 254 335 448 285
Cuts at: 2060 2210 2465 2634 2715 2771 2933 3092 3187
Size: 150 255 169 81 56 162 159 95
Cuts at: 3187 3374 3877 4098
Size: 187 503 221
Fragments arranged by size:
503 448 412 335 285 255 254 221
187 169 164 162 159 150 145 95
81 56 17
BspEI T'CCGG_A
Cuts at: 0 1334 4098
Size: 1334 2764
BspHI T'CATG_A
Cuts at: 0 173 2363 4098
Size: 173 2190 1735
Fragments arranged by size:
2190 1735 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 271 896 965 1226 3060 3096 3578 4098
Size: 271 625 69 261 1834 36 482 520
Fragments arranged by size:
1834 625 520 482 271 261 69 36
BsrBI CCG'CTC
Cuts at: 0 1867 2127 2136 2369 4044 4098
Size: 1867 260 9 233 1675 54
Fragments arranged by size:
1867 1675 260 233 54 9
BsrFI r'CCGG_y
Cuts at: 0 54 372 838 1081 1183 1661 2168 2251
Size: 54 318 466 243 102 478 507 83
Cuts at: 2251 2525 2588 3296 3428 3437 3850 4098
Size: 274 63 708 132 9 413 248
Fragments arranged by size:
708 507 478 466 413 318 274 248
243 132 102 83 63 54 9
BsrGI T'GTAC_A
Cuts at: 0 71 1424 3278 4098
Size: 71 1353 1854 820
Fragments arranged by size:
1854 1353 820 71
BssHII G'CGCG_C
Cuts at: 0 810 1738 3506 3992 4098
Size: 810 928 1768 486 106
Fragments arranged by size:
1768 928 810 486 106
BssSI C'ACGA_G
Cuts at: 0 784 2380 4098
Size: 784 1596 1718
Fragments arranged by size:
1718 1596 784
BstAPI GCAn_nnn'nTGC
Cuts at: 0 531 2151 2823 3474 3579 3660 4098
Size: 531 1620 672 651 105 81 438
Fragments arranged by size:
1620 672 651 531 438 105 81
BstEII G'GTnAC_C
Cuts at: 0 3348 4098
Size: 3348 750
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1084 1197 2616 3171 4098
Size: 1084 113 1419 555 927
Fragments arranged by size:
1419 1084 927 555 113
BstYI r'GATC_y
Cuts at: 0 1112 1475 2077 2148 3050 3623 4098
Size: 1112 363 602 71 902 573 475
Fragments arranged by size:
1112 902 602 573 475 363 71
Bsu36I CC'TnA_GG
Cuts at: 0 2344 4098
Size: 2344 1754
BtgI C'CryG_G
Cuts at: 0 20 230 329 974 1385 1400 1727 2482
Size: 20 210 99 645 411 15 327 755
Cuts at: 2482 2640 2657 3135 3219 3258 3353 4098
Size: 158 17 478 84 39 95 745
Fragments arranged by size:
755 745 645 478 411 327 210 158
99 95 84 39 20 17 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 845 966 1413 1629 3516 4098
Size: 845 121 447 216 1887 582
Fragments arranged by size:
1887 845 582 447 216 121
BtsI GCAGTG_nn'
Cuts at: 0 14 105 3405 3432 4098
Size: 14 91 3300 27 666
Fragments arranged by size:
3300 666 91 27 14
DrdI GACnn_nn'nnGTC
Cuts at: 0 1411 2035 2437 4098
Size: 1411 624 402 1661
Fragments arranged by size:
1661 1411 624 402
EaeI y'GGCC_r
Cuts at: 0 233 863 894 1086 1397 1559 1793 2384
Size: 233 630 31 192 311 162 234 591
Cuts at: 2384 2514 2576 2606 2844 3261 3294 3545 3791
Size: 130 62 30 238 417 33 251 246
Cuts at: 3791 4098
Size: 307
Fragments arranged by size:
630 591 417 311 307 251 246 238
234 233 192 162 130 62 33 31
30
EagI C'GGCC_G
Cuts at: 0 894 1559 1793 2514 3294 3791 4098
Size: 894 665 234 721 780 497 307
Fragments arranged by size:
894 780 721 665 497 307 234
EarI CTCTTCn'nnn_
Cuts at: 0 1048 1363 4098
Size: 1048 315 2735
Fragments arranged by size:
2735 1048 315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 171 592 1047 2257 3262 3319 4098
Size: 171 421 455 1210 1005 57 779
Fragments arranged by size:
1210 1005 779 455 421 171 57
EcoICRI GAG'CTC
Cuts at: 0 179 2208 4098
Size: 179 2029 1890
Fragments arranged by size:
2029 1890 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 228 898 1597 1820 2143 2161 2388
Size: 138 90 670 699 223 323 18 227
Cuts at: 2388 2638 2804 2944 3163 3331 3607 3967 4098
Size: 250 166 140 219 168 276 360 131
Fragments arranged by size:
699 670 360 323 276 250 227 223
219 168 166 140 138 131 90 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 388 4098
Size: 388 3710
EcoO109I rG'GnC_Cy
Cuts at: 0 13 734 735 789 1536 2118 2630 2631
Size: 13 721 1 54 747 582 512 1
Cuts at: 2631 2712 2790 2820 3184 3315 4098
Size: 81 78 30 364 131 783
Fragments arranged by size:
783 747 721 582 512 364 131 81
78 54 30 13 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2376 2703 4098
Size: 2376 327 1395
Fragments arranged by size:
2376 1395 327
HaeII r_GCGC'y
Cuts at: 0 98 1262 1385 1626 2361 2457 2492 2589
Size: 98 1164 123 241 735 96 35 97
Cuts at: 2589 2616 3178 3422 3700 3745 3835 4085 4098
Size: 27 562 244 278 45 90 250 13
Fragments arranged by size:
1164 735 562 278 250 244 241 123
98 97 96 90 45 35 27 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 1987 2019 2062 2094 4098
Size: 325 32 1630 32 43 32 2004
Fragments arranged by size:
2004 1630 325 43 32 32 32
HincII GTy'rAC
Cuts at: 0 2193 4098
Size: 2193 1905
KasI G'GCGC_C
Cuts at: 0 1381 2453 3696 3831 4081 4098
Size: 1381 1072 1243 135 250 17
Fragments arranged by size:
1381 1243 1072 250 135 17
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 329 950 1955 2555 4098
Size: 323 6 621 1005 600 1543
Fragments arranged by size:
1543 1005 621 600 323 6
MscI TGG'CCA
Cuts at: 0 235 1088 1399 2386 2608 4098
Size: 235 853 311 987 222 1490
Fragments arranged by size:
1490 987 853 311 235 222
MslI CAynn'nnrTG
Cuts at: 0 985 1195 1405 2986 4098
Size: 985 210 210 1581 1112
Fragments arranged by size:
1581 1112 985 210 210
MspA1I CmG'CkG
Cuts at: 0 731 803 1418 1482 1798 1936 2220 2235
Size: 731 72 615 64 316 138 284 15
Cuts at: 2235 2451 3137 3221 3236 3260 3309 3355 3738
Size: 216 686 84 15 24 49 46 383
Cuts at: 3738 3837 3870 4098
Size: 99 33 228
Fragments arranged by size:
731 686 615 383 316 284 228 216
138 99 84 72 64 49 46 33
24 15 15
NaeI GCC'GGC
Cuts at: 0 56 374 840 1185 1663 2170 3298 3439
Size: 56 318 466 345 478 507 1128 141
Cuts at: 3439 3852 4098
Size: 413 246
Fragments arranged by size:
1128 507 478 466 413 345 318 246
141 56
NarI GG'CG_CC
Cuts at: 0 1382 2454 3697 3832 4082 4098
Size: 1382 1072 1243 135 250 16
Fragments arranged by size:
1382 1243 1072 250 135 16
NcoI C'CATG_G
Cuts at: 0 20 1385 1400 4098
Size: 20 1365 15 2698
Fragments arranged by size:
2698 1365 20 15
NgoMIV G'CCGG_C
Cuts at: 0 54 372 838 1183 1661 2168 3296 3437
Size: 54 318 466 345 478 507 1128 141
Cuts at: 3437 3850 4098
Size: 413 248
Fragments arranged by size:
1128 507 478 466 413 345 318 248
141 54
NspI r_CATG'y
Cuts at: 0 1425 3498 3957 4098
Size: 1425 2073 459 141
Fragments arranged by size:
2073 1425 459 141
PasI CC'CwG_GG
Cuts at: 0 2772 3374 3705 4098
Size: 2772 602 331 393
Fragments arranged by size:
2772 602 393 331
PflMI CCAn_nnn'nTGG
Cuts at: 0 404 2238 4098
Size: 404 1834 1860
Fragments arranged by size:
1860 1834 404
PfoI T'CCnGG_A
Cuts at: 0 875 1115 2120 2804 2854 3023 4098
Size: 875 240 1005 684 50 169 1075
Fragments arranged by size:
1075 1005 875 684 240 169 50
PmlI CAC'GTG
Cuts at: 0 2337 4098
Size: 2337 1761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 2575 2607 4098
Size: 112 32 2431 32 1491
Fragments arranged by size:
2431 1491 112 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 789 2118 2790 4098
Size: 789 1329 672 1308
Fragments arranged by size:
1329 1308 789 672
PshAI GACnn'nnGTC
Cuts at: 0 1163 4098
Size: 1163 2935
PspOMI G'GGCC_C
Cuts at: 0 13 734 2630 2711 3183 4098
Size: 13 721 1896 81 472 915
Fragments arranged by size:
1896 915 721 472 81 13
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1276 1308 4098
Size: 1276 32 2790
Fragments arranged by size:
2790 1276 32
PstI C_TGCA'G
Cuts at: 0 92 752 1223 1724 2096 3412 3470 3575
Size: 92 660 471 501 372 1316 58 105
Cuts at: 3575 3656 4098
Size: 81 442
Fragments arranged by size:
1316 660 501 471 442 372 105 92
81 58
PvuII CAG'CTG
Cuts at: 0 2220 3236 3309 4098
Size: 2220 1016 73 789
Fragments arranged by size:
2220 1016 789 73
SacI G_AGCT'C
Cuts at: 0 181 2210 4098
Size: 181 2029 1888
Fragments arranged by size:
2029 1888 181
SacII CC_GC'GG
Cuts at: 0 3138 3222 3261 3356 4098
Size: 3138 84 39 95 742
Fragments arranged by size:
3138 742 95 84 39
SanDI GG'GwC_CC
Cuts at: 0 2790 4098
Size: 2790 1308
SbfI CC_TGCA'GG
Cuts at: 0 3470 4098
Size: 3470 628
ScaI AGT'ACT
Cuts at: 0 2956 4098
Size: 2956 1142
SexAI A'CCwGG_T
Cuts at: 0 389 2534 4098
Size: 389 2145 1564
Fragments arranged by size:
2145 1564 389
SfcI C'TryA_G
Cuts at: 0 88 748 1219 1720 2092 3408 3466 3571
Size: 88 660 471 501 372 1316 58 105
Cuts at: 3571 3652 3780 4098
Size: 81 128 318
Fragments arranged by size:
1316 660 501 471 372 318 128 105
88 81 58
SfoI GGC'GCC
Cuts at: 0 1383 2455 3698 3833 4083 4098
Size: 1383 1072 1243 135 250 15
Fragments arranged by size:
1383 1243 1072 250 135 15
SgrAI Cr'CCGG_yG
Cuts at: 0 54 4098
Size: 54 4044
SmaI CCC'GGG
Cuts at: 0 2115 2710 3087 4098
Size: 2115 595 377 1011
Fragments arranged by size:
2115 1011 595 377
SmlI C'TyrA_G
Cuts at: 0 934 4098
Size: 934 3164
StuI AGG'CCT
Cuts at: 0 1135 2401 3799 4098
Size: 1135 1266 1398 299
Fragments arranged by size:
1398 1266 1135 299
StyI C'CwwG_G
Cuts at: 0 20 1385 1400 1652 2471 3365 3593 4098
Size: 20 1365 15 252 819 894 228 505
Fragments arranged by size:
1365 894 819 505 252 228 20 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 662 2536 4098
Size: 445 217 1874 1562
Fragments arranged by size:
1874 1562 445 217
TatI w'GTAC_w
Cuts at: 0 71 1424 2681 2954 3278 4098
Size: 71 1353 1257 273 324 820
Fragments arranged by size:
1353 1257 820 324 273 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1822 1854 2467 2499 4098
Size: 1822 32 613 32 1599
Fragments arranged by size:
1822 1599 613 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2035 4098
Size: 2035 2063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1392 2779 3240 4098
Size: 1392 1387 461 858
Fragments arranged by size:
1392 1387 858 461
XmaI C'CCGG_G
Cuts at: 0 2113 2708 3085 4098
Size: 2113 595 377 1013
Fragments arranged by size:
2113 1013 595 377
XmnI GAAnn'nnTTC
Cuts at: 0 124 1546 4098
Size: 124 1422 2552
Fragments arranged by size:
2552 1422 124
ZraI GAC'GTC
Cuts at: 0 3552 4098
Size: 3552 546
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI
SacII SanDI SbfI ScaI SexAI SfcI SfoI SgrAI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AleI ApoI AscI AseI AsiSI
AvrII BaeI BclI BfrBI BmtI BsaBI BsiWI BsrDI
BstBI BstZ17I ClaI CspCI DraI DraIII EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PsiI PspXI PvuI RsrII SalI SapI SfiI SgfI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI