Restriction map for
pF1KSDA0962
(Linear) (Six-base) MAPSORT of: KIAA0962.seq Check: 2662 from: 1 to: 2346
KIAA0962.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 1265 1934 2346
Size: 1265 669 412
Fragments arranged by size:
1265 669 412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1502 2346
Size: 1502 844
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1304 2346
Size: 1304 1042
ApoI r'AATT_y
Cuts at: 0 62 351 1423 1890 1918 2017 2346
Size: 62 289 1072 467 28 99 329
Fragments arranged by size:
1072 467 329 289 99 62 28
AvaI C'yCGr_G
Cuts at: 0 158 1025 2213 2346
Size: 158 867 1188 133
Fragments arranged by size:
1188 867 158 133
AvrII C'CTAG_G
Cuts at: 0 99 627 654 2346
Size: 99 528 27 1692
Fragments arranged by size:
1692 528 99 27
BamHI G'GATC_C
Cuts at: 0 2239 2346
Size: 2239 107
BanII G_rGCy'C
Cuts at: 0 2305 2346
Size: 2305 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 206 724 1400 1741 2346
Size: 206 518 676 341 605
Fragments arranged by size:
676 605 518 341 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 510 544 1495 1529 1644 1678 2346
Size: 510 34 951 34 115 34 668
Fragments arranged by size:
951 668 510 115 34 34 34
BclI T'GATC_A
Cuts at: 0 267 2346
Size: 267 2079
BglI GCCn_nnn'nGGC
Cuts at: 0 124 2346
Size: 124 2222
Bme1580I G_kGCm'C
Cuts at: 0 636 2346
Size: 636 1710
BmrI ACTGGGnnnn_n'
Cuts at: 0 1748 1955 2346
Size: 1748 207 391
Fragments arranged by size:
1748 391 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 305 1617 1694 2317 2346
Size: 212 93 1312 77 623 29
Fragments arranged by size:
1312 623 212 93 77 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 2009 2346
Size: 1223 786 337
Fragments arranged by size:
1223 786 337
BsaHI Gr'CG_yC
Cuts at: 0 612 2346
Size: 612 1734
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1401 1431 2346
Size: 1401 30 915
Fragments arranged by size:
1401 915 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2170 2346
Size: 2170 176
BseYI C'CCAG_C
Cuts at: 0 598 1571 2346
Size: 598 973 775
Fragments arranged by size:
973 775 598
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2196 2346
Size: 2196 150
BsiEI CG_ry'CG
Cuts at: 0 1138 2346
Size: 1138 1208
BsmI GAATG_Cn'
Cuts at: 0 521 2346
Size: 521 1825
BsmBI CGTCTCn'nnnn_
Cuts at: 0 272 603 2346
Size: 272 331 1743
Fragments arranged by size:
1743 331 272
Bsp1286I G_dGCh'C
Cuts at: 0 636 2305 2346
Size: 636 1669 41
Fragments arranged by size:
1669 636 41
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1842 2346
Size: 1842 504
BstYI r'GATC_y
Cuts at: 0 186 1461 2239 2346
Size: 186 1275 778 107
Fragments arranged by size:
1275 778 186 107
BtsI GCAGTG_nn'
Cuts at: 0 1493 2184 2346
Size: 1493 691 162
Fragments arranged by size:
1493 691 162
DraI TTT'AAA
Cuts at: 0 987 2346
Size: 987 1359
EaeI y'GGCC_r
Cuts at: 0 2106 2346
Size: 2106 240
EarI CTCTTCn'nnn_
Cuts at: 0 926 1475 1645 1654 2134 2147 2196 2346
Size: 926 549 170 9 480 13 49 150
Fragments arranged by size:
926 549 480 170 150 49 13 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 325 2346
Size: 325 2021
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 305 1502 1617 1694 2317 2346
Size: 212 93 1197 115 77 623 29
Fragments arranged by size:
1197 623 212 115 93 77 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1790 2296 2310 2346
Size: 1790 506 14 36
Fragments arranged by size:
1790 506 36 14
EcoRI G'AATT_C
Cuts at: 0 1890 2346
Size: 1890 456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1507 2136 2346
Size: 1507 629 210
Fragments arranged by size:
1507 629 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 877 909 1546 1578 1684 1716 2173 2205
Size: 877 32 637 32 106 32 457 32
Cuts at: 2205 2346
Size: 141
Fragments arranged by size:
877 637 457 141 106 32 32 32
32
HincII GTy'rAC
Cuts at: 0 866 2187 2346
Size: 866 1321 159
Fragments arranged by size:
1321 866 159
HindIII A'AGCT_T
Cuts at: 0 1755 2346
Size: 1755 591
MfeI C'AATT_G
Cuts at: 0 410 2346
Size: 410 1936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 2346
Size: 56 2290
MscI TGG'CCA
Cuts at: 0 2108 2346
Size: 2108 238
MslI CAynn'nnrTG
Cuts at: 0 344 1868 1870 2346
Size: 344 1524 2 476
Fragments arranged by size:
1524 476 344 2
MspA1I CmG'CkG
Cuts at: 0 1336 1452 1955 2346
Size: 1336 116 503 391
Fragments arranged by size:
1336 503 391 116
NspI r_CATG'y
Cuts at: 0 1264 2346
Size: 1264 1082
PfoI T'CCnGG_A
Cuts at: 0 189 2346
Size: 189 2157
PsiI TTA'TAA
Cuts at: 0 146 2346
Size: 146 2200
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 283 315 2346
Size: 283 32 2031
Fragments arranged by size:
2031 283 32
PvuII CAG'CTG
Cuts at: 0 1452 1955 2346
Size: 1452 503 391
Fragments arranged by size:
1452 503 391
SapI GCTCTTCn'nnn_
Cuts at: 0 1475 2346
Size: 1475 871
ScaI AGT'ACT
Cuts at: 0 1156 2346
Size: 1156 1190
SfcI C'TryA_G
Cuts at: 0 1266 1912 2346
Size: 1266 646 434
Fragments arranged by size:
1266 646 434
SmaI CCC'GGG
Cuts at: 0 160 2346
Size: 160 2186
SmlI C'TyrA_G
Cuts at: 0 1238 2024 2213 2346
Size: 1238 786 189 133
Fragments arranged by size:
1238 786 189 133
StuI AGG'CCT
Cuts at: 0 1005 2346
Size: 1005 1341
StyI C'CwwG_G
Cuts at: 0 99 627 654 1399 2235 2346
Size: 99 528 27 745 836 111
Fragments arranged by size:
836 745 528 111 99 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 562 1519 2194 2346
Size: 562 957 675 152
Fragments arranged by size:
957 675 562 152
TatI w'GTAC_w
Cuts at: 0 1154 2091 2346
Size: 1154 937 255
Fragments arranged by size:
1154 937 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 572 604 1143 1175 1361 1393 1395 1401
Size: 572 32 539 32 186 32 2 6
Cuts at: 1401 1427 1433 2346
Size: 26 6 913
Fragments arranged by size:
913 572 539 186 32 32 32 26
6 6 2
XbaI T'CTAG_A
Cuts at: 0 1980 2346
Size: 1980 366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1735 2346
Size: 1735 611
XhoI C'TCGA_G
Cuts at: 0 2213 2346
Size: 2213 133
XmaI C'CCGG_G
Cuts at: 0 158 2346
Size: 158 2188
XmnI GAAnn'nnTTC
Cuts at: 0 458 551 731 1108 2119 2346
Size: 458 93 180 377 1011 227
Fragments arranged by size:
1011 458 377 227 180 93
Enzymes that do cut:
AccI AcuI AlfI ApoI AvaI AvrII BamHI BanII
BbsI BcgI BclI BglI Bme1580I BmrI BpmI BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI BsmBI
Bsp1286I BstXI BstYI BtsI DraI EaeI EarI EciI
Eco57MI EcoNI EcoRI FalI Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NspI PfoI PsiI PsrI
PvuII SapI ScaI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BanI BbeI BbvCI BciVI BfrBI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaWI BsiHKAI BsiWI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EcoICRI EcoO109I EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I ZraI