Restriction map for
pF1KA0947
(Linear) (Six-base) MAPSORT of: KIAA0947.seq Check: 5814 from: 1 to: 6798
KIAA0947.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 668 6798
Size: 668 6130
AatII G_ACGT'C
Cuts at: 0 3460 6798
Size: 3460 3338
AccI GT'mk_AC
Cuts at: 0 5518 6478 6633 6798
Size: 5518 960 155 165
Fragments arranged by size:
5518 960 165 155
Acc65I G'GTAC_C
Cuts at: 0 3274 6798
Size: 3274 3524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1658 3200 3842 3929 4694 5093 5384
Size: 643 1015 1542 642 87 765 399 291
Cuts at: 5384 5654 5776 6798
Size: 270 122 1022
Fragments arranged by size:
1542 1022 1015 765 643 642 399 291
270 122 87
AflIII A'CryG_T
Cuts at: 0 4302 6101 6798
Size: 4302 1799 697
Fragments arranged by size:
4302 1799 697
AgeI A'CCGG_T
Cuts at: 0 2686 6798
Size: 2686 4112
AhdI GACnn_n'nnGTC
Cuts at: 0 5556 6798
Size: 5556 1242
AleI CACnn'nnGTG
Cuts at: 0 2070 2378 2749 3019 6798
Size: 2070 308 371 270 3779
Fragments arranged by size:
3779 2070 371 308 270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 443 3019 3360 3871 5165 6173 6798
Size: 443 2576 341 511 1294 1008 625
Fragments arranged by size:
2576 1294 1008 625 511 443 341
AlwNI CAG_nnn'CTG
Cuts at: 0 1744 3412 4096 4947 5410 6798
Size: 1744 1668 684 851 463 1388
Fragments arranged by size:
1744 1668 1388 851 684 463
ApaI G_GGCC'C
Cuts at: 0 5289 6798
Size: 5289 1509
ApoI r'AATT_y
Cuts at: 0 84 242 490 520 569 952 1158 2536
Size: 84 158 248 30 49 383 206 1378
Cuts at: 2536 2841 3393 3816 3891 5238 5352 5872 5936
Size: 305 552 423 75 1347 114 520 64
Cuts at: 5936 6292 6310 6689 6798
Size: 356 18 379 109
Fragments arranged by size:
1378 1347 552 520 423 383 379 356
305 248 206 158 114 109 84 75
64 49 30 18
AseI AT'TA_AT
Cuts at: 0 109 1706 2311 6137 6798
Size: 109 1597 605 3826 661
Fragments arranged by size:
3826 1597 661 605 109
AvaI C'yCGr_G
Cuts at: 0 31 1071 1324 1514 6798
Size: 31 1040 253 190 5284
Fragments arranged by size:
5284 1040 253 190 31
BanI G'GyrC_C
Cuts at: 0 27 2372 3193 3274 6798
Size: 27 2345 821 81 3524
Fragments arranged by size:
3524 2345 821 81 27
BanII G_rGCy'C
Cuts at: 0 859 2122 2282 5289 6030 6798
Size: 859 1263 160 3007 741 768
Fragments arranged by size:
3007 1263 859 768 741 160
BbeI G_GCGC'C
Cuts at: 0 31 6798
Size: 31 6767
BbsI GAAGACnn'nnnn_
Cuts at: 0 505 1079 1501 1931 2446 4088 6326 6798
Size: 505 574 422 430 515 1642 2238 472
Fragments arranged by size:
2238 1642 574 515 505 472 430 422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1505 1539 6798
Size: 1505 34 5259
Fragments arranged by size:
5259 1505 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 324 3243 5276 6798
Size: 324 2919 2033 1522
Fragments arranged by size:
2919 2033 1522 324
BclI T'GATC_A
Cuts at: 0 960 966 2148 2333 4578 4611 6798
Size: 960 6 1182 185 2245 33 2187
Fragments arranged by size:
2245 2187 1182 960 185 33 6
BfrBI ATG'CAT
Cuts at: 0 1526 6798
Size: 1526 5272
BglII A'GATC_T
Cuts at: 0 984 2970 3076 6798
Size: 984 1986 106 3722
Fragments arranged by size:
3722 1986 984 106
Bme1580I G_kGCm'C
Cuts at: 0 1043 5289 6777 6798
Size: 1043 4246 1488 21
Fragments arranged by size:
4246 1488 1043 21
BmgBI CAC'GTC
Cuts at: 0 5068 6798
Size: 5068 1730
BmrI ACTGGGnnnn_n'
Cuts at: 0 283 1929 4381 5020 5435 5564 6798
Size: 283 1646 2452 639 415 129 1234
Fragments arranged by size:
2452 1646 1234 639 415 283 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 398 4005 6798
Size: 398 3607 2793
Fragments arranged by size:
3607 2793 398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 843 2801 2964 4355 5063 5612 6798
Size: 702 141 1958 163 1391 708 549 1186
Fragments arranged by size:
1958 1391 1186 708 702 549 163 141
Bpu10I CC'TnA_GC
Cuts at: 0 404 6778 6798
Size: 404 6374 20
Fragments arranged by size:
6374 404 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1781 2136 2652 3556 6216 6687 6798
Size: 931 850 355 516 904 2660 471 111
Fragments arranged by size:
2660 931 904 850 516 471 355 111
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 3423 6798
Size: 7 3416 3375
Fragments arranged by size:
3416 3375 7
BsaBI GATnn'nnATC
Cuts at: 0 335 965 983 4241 6555 6798
Size: 335 630 18 3258 2314 243
Fragments arranged by size:
3258 2314 630 335 243 18
BsaHI Gr'CG_yC
Cuts at: 0 28 3457 6798
Size: 28 3429 3341
Fragments arranged by size:
3429 3341 28
BsaWI w'CCGG_w
Cuts at: 0 2067 2686 6066 6798
Size: 2067 619 3380 732
Fragments arranged by size:
3380 2067 732 619
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 732 762 3066 3096 4818 4848 6798
Size: 732 30 2304 30 1722 30 1950
Fragments arranged by size:
2304 1950 1722 732 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 239 1013 3921 4901 5066 5126 6798
Size: 239 774 2908 980 165 60 1672
Fragments arranged by size:
2908 1672 980 774 239 165 60
BseYI C'CCAG_C
Cuts at: 0 6673 6798
Size: 6673 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 1535 2950 5705 6798
Size: 805 730 1415 2755 1093
Fragments arranged by size:
2755 1415 1093 805 730
BsiHKAI G_wGCw'C
Cuts at: 0 859 2282 4120 4507 6030 6784 6798
Size: 859 1423 1838 387 1523 754 14
Fragments arranged by size:
1838 1523 1423 859 754 387 14
BsmI GAATG_Cn'
Cuts at: 0 370 3296 6228 6798
Size: 370 2926 2932 570
Fragments arranged by size:
2932 2926 570 370
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3305 5138 6798
Size: 3305 1833 1660
Fragments arranged by size:
3305 1833 1660
Bsp1286I G_dGCh'C
Cuts at: 0 859 1043 2122 2282 4120 4507 5289 6030
Size: 859 184 1079 160 1838 387 782 741
Cuts at: 6030 6777 6784 6798
Size: 747 7 14
Fragments arranged by size:
1838 1079 859 782 747 741 387 184
160 14 7
BspEI T'CCGG_A
Cuts at: 0 6066 6798
Size: 6066 732
BspHI T'CATG_A
Cuts at: 0 963 6798
Size: 963 5835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 668 705 4094 5373 6798
Size: 668 37 3389 1279 1425
Fragments arranged by size:
3389 1425 1279 668 37
BsrDI GCAATG_nn'
Cuts at: 0 2257 2471 3837 6152 6470 6798
Size: 2257 214 1366 2315 318 328
Fragments arranged by size:
2315 2257 1366 328 318 214
BsrFI r'CCGG_y
Cuts at: 0 2686 6798
Size: 2686 4112
BssSI C'ACGA_G
Cuts at: 0 350 5327 6798
Size: 350 4977 1471
Fragments arranged by size:
4977 1471 350
BstAPI GCAn_nnn'nTGC
Cuts at: 0 5410 6472 6798
Size: 5410 1062 326
Fragments arranged by size:
5410 1062 326
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2973 6702 6798
Size: 2973 3729 96
Fragments arranged by size:
3729 2973 96
BstYI r'GATC_y
Cuts at: 0 277 984 2265 2970 3076 5082 6386 6698
Size: 277 707 1281 705 106 2006 1304 312
Cuts at: 6698 6798
Size: 100
Fragments arranged by size:
2006 1304 1281 707 705 312 277 106
100
BstZ17I GTA'TAC
Cuts at: 0 6479 6634 6798
Size: 6479 155 164
Fragments arranged by size:
6479 164 155
Bsu36I CC'TnA_GG
Cuts at: 0 2960 5231 6798
Size: 2960 2271 1567
Fragments arranged by size:
2960 2271 1567
BtgI C'CryG_G
Cuts at: 0 809 5047 6625 6798
Size: 809 4238 1578 173
Fragments arranged by size:
4238 1578 809 173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 69 6798
Size: 69 6729
BtsI GCAGTG_nn'
Cuts at: 0 2166 2546 2933 3003 3354 4500 5585 6798
Size: 2166 380 387 70 351 1146 1085 1213
Fragments arranged by size:
2166 1213 1146 1085 387 380 351 70
ClaI AT'CG_AT
Cuts at: 0 4662 6798
Size: 4662 2136
DraI TTT'AAA
Cuts at: 0 284 317 2039 3896 6798
Size: 284 33 1722 1857 2902
Fragments arranged by size:
2902 1857 1722 284 33
DraIII CAC_nnn'GTG
Cuts at: 0 222 3758 4322 4324 5200 6798
Size: 222 3536 564 2 876 1598
Fragments arranged by size:
3536 1598 876 564 222 2
EaeI y'GGCC_r
Cuts at: 0 1746 4998 5050 5153 5184 5208 6798
Size: 1746 3252 52 103 31 24 1590
Fragments arranged by size:
3252 1746 1590 103 52 31 24
EarI CTCTTCn'nnn_
Cuts at: 0 189 221 293 367 1031 1902 2149 3692
Size: 189 32 72 74 664 871 247 1543
Cuts at: 3692 6798
Size: 3106
Fragments arranged by size:
3106 1543 871 664 247 189 74 72
32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 5324 6729 6798
Size: 58 5266 1405 69
Fragments arranged by size:
5266 1405 69 58
EcoICRI GAG'CTC
Cuts at: 0 857 2280 6028 6798
Size: 857 1423 3748 770
Fragments arranged by size:
3748 1423 857 770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 702 843 1658 2801 2964 3200 3842
Size: 643 59 141 815 1143 163 236 642
Cuts at: 3842 3929 4355 4694 5063 5093 5384 5612 5654
Size: 87 426 339 369 30 291 228 42
Cuts at: 5654 5776 6798
Size: 122 1022
Fragments arranged by size:
1143 1022 815 643 642 426 369 339
291 236 228 163 141 122 87 59
42 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 668 6798
Size: 668 6130
EcoO109I rG'GnC_Cy
Cuts at: 0 773 3090 4885 5058 5227 5285 5362 5558
Size: 773 2317 1795 173 169 58 77 196
Cuts at: 5558 6798
Size: 1240
Fragments arranged by size:
2317 1795 1240 773 196 173 169 77
58
EcoRI G'AATT_C
Cuts at: 0 2536 5238 6798
Size: 2536 2702 1560
Fragments arranged by size:
2702 2536 1560
EcoRV GAT'ATC
Cuts at: 0 4961 5948 6798
Size: 4961 987 850
Fragments arranged by size:
4961 987 850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 250 843 3700 6798
Size: 250 593 2857 3098
Fragments arranged by size:
3098 2857 593 250
HaeII r_GCGC'y
Cuts at: 0 31 6798
Size: 31 6767
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1126 1158 1522 1554 1825 1857 2290 2322
Size: 1126 32 364 32 271 32 433 32
Cuts at: 2322 2433 2465 2962 2994 3447 3479 4551 4583
Size: 111 32 497 32 453 32 1072 32
Cuts at: 4583 4956 4988 5542 5574 6798
Size: 373 32 554 32 1224
Fragments arranged by size:
1224 1126 1072 554 497 453 433 373
364 271 111 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 152 2696 5513 6798
Size: 152 2544 2817 1285
Fragments arranged by size:
2817 2544 1285 152
HindIII A'AGCT_T
Cuts at: 0 6707 6798
Size: 6707 91
HpaI GTT'AAC
Cuts at: 0 152 6798
Size: 152 6646
KasI G'GCGC_C
Cuts at: 0 27 6798
Size: 27 6771
KpnI G_GTAC'C
Cuts at: 0 3278 6798
Size: 3278 3520
MfeI C'AATT_G
Cuts at: 0 998 6532 6798
Size: 998 5534 266
Fragments arranged by size:
5534 998 266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 6798
Size: 775 6023
MscI TGG'CCA
Cuts at: 0 1748 5000 5052 5210 6798
Size: 1748 3252 52 158 1588
Fragments arranged by size:
3252 1748 1588 158 52
MslI CAynn'nnrTG
Cuts at: 0 2070 2378 2749 2996 3019 4182 4387 4739
Size: 2070 308 371 247 23 1163 205 352
Cuts at: 4739 5810 6798
Size: 1071 988
Fragments arranged by size:
2070 1163 1071 988 371 352 308 247
205 23
MspA1I CmG'CkG
Cuts at: 0 3059 3967 4001 5632 6755 6798
Size: 3059 908 34 1631 1123 43
Fragments arranged by size:
3059 1631 1123 908 43 34
NarI GG'CG_CC
Cuts at: 0 28 6798
Size: 28 6770
NcoI C'CATG_G
Cuts at: 0 809 5047 6625 6798
Size: 809 4238 1578 173
Fragments arranged by size:
4238 1578 809 173
NdeI CA'TA_TG
Cuts at: 0 585 6798
Size: 585 6213
NruI TCG'CGA
Cuts at: 0 54 6798
Size: 54 6744
NsiI A_TGCA'T
Cuts at: 0 1528 6798
Size: 1528 5270
NspI r_CATG'y
Cuts at: 0 794 3943 4306 6105 6466 6607 6798
Size: 794 3149 363 1799 361 141 191
Fragments arranged by size:
3149 1799 794 363 361 191 141
PasI CC'CwG_GG
Cuts at: 0 1573 5614 6798
Size: 1573 4041 1184
Fragments arranged by size:
4041 1573 1184
PciI A'CATG_T
Cuts at: 0 4302 6101 6798
Size: 4302 1799 697
Fragments arranged by size:
4302 1799 697
PfoI T'CCnGG_A
Cuts at: 0 4332 6701 6798
Size: 4332 2369 97
Fragments arranged by size:
4332 2369 97
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 620 652 6798
Size: 620 32 6146
Fragments arranged by size:
6146 620 32
PpuMI rG'GwC_Cy
Cuts at: 0 773 5058 5362 5558 6798
Size: 773 4285 304 196 1240
Fragments arranged by size:
4285 1240 773 304 196
PshAI GACnn'nnGTC
Cuts at: 0 6668 6798
Size: 6668 130
PsiI TTA'TAA
Cuts at: 0 4216 4228 4625 6798
Size: 4216 12 397 2173
Fragments arranged by size:
4216 2173 397 12
PspOMI G'GGCC_C
Cuts at: 0 5285 6798
Size: 5285 1513
PspXI vC'TCGA_Gb
Cuts at: 0 1514 6798
Size: 1514 5284
PstI C_TGCA'G
Cuts at: 0 80 185 2057 2928 3349 5106 5144 5592
Size: 80 105 1872 871 421 1757 38 448
Cuts at: 5592 5983 6798
Size: 391 815
Fragments arranged by size:
1872 1757 871 815 448 421 391 105
80 38
PvuII CAG'CTG
Cuts at: 0 3059 3967 5632 6755 6798
Size: 3059 908 1665 1123 43
Fragments arranged by size:
3059 1665 1123 908 43
SacI G_AGCT'C
Cuts at: 0 859 2282 6030 6798
Size: 859 1423 3748 768
Fragments arranged by size:
3748 1423 859 768
SanDI GG'GwC_CC
Cuts at: 0 5558 6798
Size: 5558 1240
SapI GCTCTTCn'nnn_
Cuts at: 0 2149 6798
Size: 2149 4649
ScaI AGT'ACT
Cuts at: 0 948 2364 3486 6798
Size: 948 1416 1122 3312
Fragments arranged by size:
3312 1416 1122 948
SexAI A'CCwGG_T
Cuts at: 0 663 4310 6798
Size: 663 3647 2488
Fragments arranged by size:
3647 2488 663
SfcI C'TryA_G
Cuts at: 0 76 181 253 2053 2078 2531 2699 2924
Size: 76 105 72 1800 25 453 168 225
Cuts at: 2924 3345 3416 4838 5102 5140 5588 5979 6042
Size: 421 71 1422 264 38 448 391 63
Cuts at: 6042 6798
Size: 756
Fragments arranged by size:
1800 1422 756 453 448 421 391 264
225 168 105 76 72 71 63 38
25
SfoI GGC'GCC
Cuts at: 0 29 6798
Size: 29 6769
SmaI CCC'GGG
Cuts at: 0 33 1073 6798
Size: 33 1040 5725
Fragments arranged by size:
5725 1040 33
SmlI C'TyrA_G
Cuts at: 0 910 1324 1514 1796 2115 2631 3535 6195
Size: 910 414 190 282 319 516 904 2660
Cuts at: 6195 6666 6798
Size: 471 132
Fragments arranged by size:
2660 910 904 516 471 414 319 282
190 132
SphI G_CATG'C
Cuts at: 0 6607 6798
Size: 6607 191
SspI AAT'ATT
Cuts at: 0 5316 6573 6798
Size: 5316 1257 225
Fragments arranged by size:
5316 1257 225
StuI AGG'CCT
Cuts at: 0 6724 6798
Size: 6724 74
StyI C'CwwG_G
Cuts at: 0 809 1602 1904 3971 4930 5047 6625 6798
Size: 809 793 302 2067 959 117 1578 173
Fragments arranged by size:
2067 1578 959 809 793 302 173 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2207 2511 6798
Size: 2207 304 4287
Fragments arranged by size:
4287 2207 304
TatI w'GTAC_w
Cuts at: 0 921 946 1482 2362 3484 6798
Size: 921 25 536 880 1122 3314
Fragments arranged by size:
3314 1122 921 880 536 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1060 1092 3066 3098 5250 5282 5316 5348
Size: 1060 32 1974 32 2152 32 34 32
Cuts at: 5348 6798
Size: 1450
Fragments arranged by size:
2152 1974 1450 1060 34 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1144 3027 4312 6798
Size: 1144 1883 1285 2486
Fragments arranged by size:
2486 1883 1285 1144
XbaI T'CTAG_A
Cuts at: 0 1765 6798
Size: 1765 5033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1942 6798
Size: 1942 4856
XhoI C'TCGA_G
Cuts at: 0 1324 1514 6798
Size: 1324 190 5284
Fragments arranged by size:
5284 1324 190
XmaI C'CCGG_G
Cuts at: 0 31 1071 6798
Size: 31 1040 5727
Fragments arranged by size:
5727 1040 31
XmnI GAAnn'nnTTC
Cuts at: 0 986 2606 5664 6062 6551 6798
Size: 986 1620 3058 398 489 247
Fragments arranged by size:
3058 1620 986 489 398 247
ZraI GAC'GTC
Cuts at: 0 3458 6798
Size: 3458 3340
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApoI AseI AvaI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MmeI MscI
MslI MspA1I NarI NcoI NdeI NruI NsiI NspI
PasI PciI PfoI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuII SacI SanDI SapI ScaI SexAI
SfcI SfoI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AloI ApaLI AscI AsiSI AvrII
BaeI BamHI BbvCI BglI BlpI BmtI BsaAI BsiEI
BsiWI BsrBI BsrGI BssHII BstBI BstEII CspCI DrdI
EagI FseI FspI FspAI MluI NaeI NgoMIV NheI
NotI PacI PflMI PmeI PmlI PsrI PvuI RsrII
SacII SalI SbfI SfiI SgfI SgrAI SnaBI SpeI
SrfI SwaI