Restriction map for
pF1KA0944
(Linear) (Six-base) MAPSORT of: KIAA0944.seq Check: 3654 from: 1 to: 12072
KIAA0944.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4207 7999 11203 12072
Size: 4207 3792 3204 869
Fragments arranged by size:
4207 3792 3204 869
AatII G_ACGT'C
Cuts at: 0 1295 12072
Size: 1295 10777
AccI GT'mk_AC
Cuts at: 0 6370 11449 12072
Size: 6370 5079 623
Fragments arranged by size:
6370 5079 623
Acc65I G'GTAC_C
Cuts at: 0 2150 4362 8854 11315 12072
Size: 2150 2212 4492 2461 757
Fragments arranged by size:
4492 2461 2212 2150 757
AclI AA'CG_TT
Cuts at: 0 264 12072
Size: 264 11808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2074 2119 2243 2902 4351 4564 5024 7009
Size: 2074 45 124 659 1449 213 460 1985
Cuts at: 7009 7535 8480 9748 10127 10444 11407 12072
Size: 526 945 1268 379 317 963 665
Fragments arranged by size:
2074 1985 1449 1268 963 945 665 659
526 460 379 317 213 124 45
AflII C'TTAA_G
Cuts at: 0 7149 8512 12072
Size: 7149 1363 3560
Fragments arranged by size:
7149 3560 1363
AflIII A'CryG_T
Cuts at: 0 558 3084 3634 4267 5264 6719 7376 7595
Size: 558 2526 550 633 997 1455 657 219
Cuts at: 7595 9184 10856 12072
Size: 1589 1672 1216
Fragments arranged by size:
2526 1672 1589 1455 1216 997 657 633
558 550 219
AgeI A'CCGG_T
Cuts at: 0 11656 12072
Size: 11656 416
AhdI GACnn_n'nnGTC
Cuts at: 0 2997 12072
Size: 2997 9075
AleI CACnn'nnGTG
Cuts at: 0 3271 3667 6604 12072
Size: 3271 396 2937 5468
Fragments arranged by size:
5468 3271 2937 396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 422 2234 4769 7420 12072
Size: 422 1812 2535 2651 4652
Fragments arranged by size:
4652 2651 2535 1812 422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3530 3562 12072
Size: 3530 32 8510
Fragments arranged by size:
8510 3530 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1177 2558 4222 5938 6680 9342 10274 11218
Size: 1177 1381 1664 1716 742 2662 932 944
Cuts at: 11218 11863 12072
Size: 645 209
Fragments arranged by size:
2662 1716 1664 1381 1177 944 932 742
645 209
ApaI G_GGCC'C
Cuts at: 0 8212 10406 12072
Size: 8212 2194 1666
Fragments arranged by size:
8212 2194 1666
ApaLI G'TGCA_C
Cuts at: 0 10280 12072
Size: 10280 1792
ApoI r'AATT_y
Cuts at: 0 749 895 1574 1765 1994 2113 2323 2746
Size: 749 146 679 191 229 119 210 423
Cuts at: 2746 2804 3319 4093 4574 4615 4740 5338 5383
Size: 58 515 774 481 41 125 598 45
Cuts at: 5383 5602 5961 6418 6430 6704 6890 6974 9729
Size: 219 359 457 12 274 186 84 2755
Cuts at: 9729 9746 10195 10336 10578 10720 10780 10981 11007
Size: 17 449 141 242 142 60 201 26
Cuts at: 11007 11492 11575 11843 11982 12072
Size: 485 83 268 139 90
Fragments arranged by size:
2755 774 749 679 598 515 485 481
457 449 423 359 274 268 242 229
219 210 201 191 186 146 142 141
139 125 119 90 84 83 60 58
45 41 26 17 12
AseI AT'TA_AT
Cuts at: 0 1666 4211 5881 7696 9890 12072
Size: 1666 2545 1670 1815 2194 2182
Fragments arranged by size:
2545 2194 2182 1815 1670 1666
AvaI C'yCGr_G
Cuts at: 0 689 6149 12072
Size: 689 5460 5923
Fragments arranged by size:
5923 5460 689
AvrII C'CTAG_G
Cuts at: 0 3895 10791 12072
Size: 3895 6896 1281
Fragments arranged by size:
6896 3895 1281
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 7470 7503 9083 9116 12072
Size: 7470 33 1580 33 2956
Fragments arranged by size:
7470 2956 1580 33 33
BamHI G'GATC_C
Cuts at: 0 6983 12072
Size: 6983 5089
BanI G'GyrC_C
Cuts at: 0 108 1455 2150 4362 8011 8854 11315 11659
Size: 108 1347 695 2212 3649 843 2461 344
Cuts at: 11659 12072
Size: 413
Fragments arranged by size:
3649 2461 2212 1347 843 695 413 344
108
BanII G_rGCy'C
Cuts at: 0 2975 3155 3951 6168 8212 9674 10406 12072
Size: 2975 180 796 2217 2044 1462 732 1666
Fragments arranged by size:
2975 2217 2044 1666 1462 796 732 180
BbsI GAAGACnn'nnnn_
Cuts at: 0 585 718 4006 7176 9449 11395 11607 11738
Size: 585 133 3288 3170 2273 1946 212 131
Cuts at: 11738 12072
Size: 334
Fragments arranged by size:
3288 3170 2273 1946 585 334 212 133
131
BciVI GTATCCnnnnn_n'
Cuts at: 0 3567 6831 9199 12072
Size: 3567 3264 2368 2873
Fragments arranged by size:
3567 3264 2873 2368
BclI T'GATC_A
Cuts at: 0 3848 7386 11061 12072
Size: 3848 3538 3675 1011
Fragments arranged by size:
3848 3675 3538 1011
BfrBI ATG'CAT
Cuts at: 0 916 2458 7441 8135 12072
Size: 916 1542 4983 694 3937
Fragments arranged by size:
4983 3937 1542 916 694
BglI GCCn_nnn'nGGC
Cuts at: 0 159 7069 12072
Size: 159 6910 5003
Fragments arranged by size:
6910 5003 159
BglII A'GATC_T
Cuts at: 0 2130 4588 10827 11454 12072
Size: 2130 2458 6239 627 618
Fragments arranged by size:
6239 2458 2130 627 618
BlpI GC'TnA_GC
Cuts at: 0 1514 2099 12072
Size: 1514 585 9973
Fragments arranged by size:
9973 1514 585
Bme1580I G_kGCm'C
Cuts at: 0 8212 10284 10406 11664 12072
Size: 8212 2072 122 1258 408
Fragments arranged by size:
8212 2072 1258 408 122
BmgBI CAC'GTC
Cuts at: 0 10219 12072
Size: 10219 1853
BmrI ACTGGGnnnn_n'
Cuts at: 0 2568 2783 3528 4004 5074 5948 8723 10956
Size: 2568 215 745 476 1070 874 2775 2233
Cuts at: 10956 12072
Size: 1116
Fragments arranged by size:
2775 2568 2233 1116 1070 874 745 476
215
BmtI G_CTAG'C
Cuts at: 0 2687 7859 12072
Size: 2687 5172 4213
Fragments arranged by size:
5172 4213 2687
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3763 12072
Size: 3763 8309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1922 2430 2449 2972 2989 3497 4889 5033
Size: 1922 508 19 523 17 508 1392 144
Cuts at: 5033 5295 6266 6494 7510 8267 8704 10328 11594
Size: 262 971 228 1016 757 437 1624 1266
Cuts at: 11594 12072
Size: 478
Fragments arranged by size:
1922 1624 1392 1266 1016 971 757 523
508 508 478 437 262 228 144 19
17
Bpu10I CC'TnA_GC
Cuts at: 0 3808 12072
Size: 3808 8264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 3259 3637 5554 7784 9757 10182 10519
Size: 504 2755 378 1917 2230 1973 425 337
Cuts at: 10519 11013 12072
Size: 494 1059
Fragments arranged by size:
2755 2230 1973 1917 1059 504 494 425
378 337
BsaI GGTCTCn'nnnn_
Cuts at: 0 5271 12072
Size: 5271 6801
BsaAI yAC'GTr
Cuts at: 0 561 2957 4451 12072
Size: 561 2396 1494 7621
Fragments arranged by size:
7621 2396 1494 561
BsaBI GATnn'nnATC
Cuts at: 0 4604 5591 12072
Size: 4604 987 6481
Fragments arranged by size:
6481 4604 987
BsaHI Gr'CG_yC
Cuts at: 0 1292 12072
Size: 1292 10780
BsaWI w'CCGG_w
Cuts at: 0 5438 8734 10686 11656 12072
Size: 5438 3296 1952 970 416
Fragments arranged by size:
5438 3296 1952 970 416
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2554 2584 3356 3386 3412 3442 3701 3731
Size: 2554 30 772 30 26 30 259 30
Cuts at: 3731 8855 8885 9171 9201 10835 10865 10919 10949
Size: 5124 30 286 30 1634 30 54 30
Cuts at: 10949 12072
Size: 1123
Fragments arranged by size:
5124 2554 1634 1123 772 286 259 54
30 30 30 30 30 30 30 30
26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1790 2610 3983 5798 6299 10528 11325 11597
Size: 1790 820 1373 1815 501 4229 797 272
Cuts at: 11597 11966 12072
Size: 369 106
Fragments arranged by size:
4229 1815 1790 1373 820 797 501 369
272 106
BseYI C'CCAG_C
Cuts at: 0 4303 4534 7252 8211 11106 11663 12072
Size: 4303 231 2718 959 2895 557 409
Fragments arranged by size:
4303 2895 2718 959 557 409 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 1079 8045 12072
Size: 1024 55 6966 4027
Fragments arranged by size:
6966 4027 1024 55
BsiEI CG_ry'CG
Cuts at: 0 458 8369 12072
Size: 458 7911 3703
Fragments arranged by size:
7911 3703 458
BsiHKAI G_wGCw'C
Cuts at: 0 1469 3155 3714 3975 4875 6965 10284 10301
Size: 1469 1686 559 261 900 2090 3319 17
Cuts at: 10301 12072
Size: 1771
Fragments arranged by size:
3319 2090 1771 1686 1469 900 559 261
17
BsmI GAATG_Cn'
Cuts at: 0 819 3119 4756 8952 9332 10222 10921 10970
Size: 819 2300 1637 4196 380 890 699 49
Cuts at: 10970 11136 12072
Size: 166 936
Fragments arranged by size:
4196 2300 1637 936 890 819 699 380
166 49
Bsp1286I G_dGCh'C
Cuts at: 0 1469 2975 3155 3714 3951 3975 4875 6168
Size: 1469 1506 180 559 237 24 900 1293
Cuts at: 6168 6965 8212 9674 10284 10301 10406 11664 12072
Size: 797 1247 1462 610 17 105 1258 408
Fragments arranged by size:
1506 1469 1462 1293 1258 1247 900 797
610 559 408 237 180 105 24 17
BspEI T'CCGG_A
Cuts at: 0 8734 10686 12072
Size: 8734 1952 1386
Fragments arranged by size:
8734 1952 1386
BspHI T'CATG_A
Cuts at: 0 947 1208 1295 2442 6627 6735 8256 9308
Size: 947 261 87 1147 4185 108 1521 1052
Cuts at: 9308 12072
Size: 2764
Fragments arranged by size:
4185 2764 1521 1147 1052 947 261 108
87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4207 5319 7999 11203 12072
Size: 4207 1112 2680 3204 869
Fragments arranged by size:
4207 3204 2680 1112 869
BsrBI CCG'CTC
Cuts at: 0 9882 11946 12072
Size: 9882 2064 126
Fragments arranged by size:
9882 2064 126
BsrDI GCAATG_nn'
Cuts at: 0 824 2471 4361 6494 7389 8402 10406 11593
Size: 824 1647 1890 2133 895 1013 2004 1187
Cuts at: 11593 12072
Size: 479
Fragments arranged by size:
2133 2004 1890 1647 1187 1013 895 824
479
BsrFI r'CCGG_y
Cuts at: 0 9812 10952 11656 12072
Size: 9812 1140 704 416
Fragments arranged by size:
9812 1140 704 416
BsrGI T'GTAC_A
Cuts at: 0 5581 8699 9958 12072
Size: 5581 3118 1259 2114
Fragments arranged by size:
5581 3118 2114 1259
BstBI TT'CG_AA
Cuts at: 0 6876 8880 12072
Size: 6876 2004 3192
Fragments arranged by size:
6876 3192 2004
BstEII G'GTnAC_C
Cuts at: 0 2584 12072
Size: 2584 9488
BstXI CCAn_nnnn'nTGG
Cuts at: 0 764 834 1978 4020 9531 9600 10357 11504
Size: 764 70 1144 2042 5511 69 757 1147
Cuts at: 11504 11618 12072
Size: 114 454
Fragments arranged by size:
5511 2042 1147 1144 764 757 454 114
70 69
BstYI r'GATC_y
Cuts at: 0 684 2130 2854 3261 4588 6983 8973 9129
Size: 684 1446 724 407 1327 2395 1990 156
Cuts at: 9129 10314 10827 11454 12072
Size: 1185 513 627 618
Fragments arranged by size:
2395 1990 1446 1327 1185 724 684 627
618 513 407 156
BstZ17I GTA'TAC
Cuts at: 0 6371 12072
Size: 6371 5701
Bsu36I CC'TnA_GG
Cuts at: 0 3011 3977 9320 10715 11756 12072
Size: 3011 966 5343 1395 1041 316
Fragments arranged by size:
5343 3011 1395 1041 966 316
BtgI C'CryG_G
Cuts at: 0 507 4952 5782 8480 10319 12072
Size: 507 4445 830 2698 1839 1753
Fragments arranged by size:
4445 2698 1839 1753 830 507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1775 2725 3791 8009 12072
Size: 1775 950 1066 4218 4063
Fragments arranged by size:
4218 4063 1775 1066 950
BtsI GCAGTG_nn'
Cuts at: 0 12 1065 2747 4415 5096 12072
Size: 12 1053 1682 1668 681 6976
Fragments arranged by size:
6976 1682 1668 1053 681 12
ClaI AT'CG_AT
Cuts at: 0 5595 12072
Size: 5595 6477
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2938 2973 5270 5305 11967 12002 12072
Size: 2938 35 2297 35 6662 35 70
Fragments arranged by size:
6662 2938 2297 70 35 35 35
DraI TTT'AAA
Cuts at: 0 680 867 4934 5667 5972 7139 12072
Size: 680 187 4067 733 305 1167 4933
Fragments arranged by size:
4933 4067 1167 733 680 305 187
DrdI GACnn_nn'nnGTC
Cuts at: 0 424 2614 5365 12072
Size: 424 2190 2751 6707
Fragments arranged by size:
6707 2751 2190 424
EaeI y'GGCC_r
Cuts at: 0 23 8039 8366 8534 11908 11970 12072
Size: 23 8016 327 168 3374 62 102
Fragments arranged by size:
8016 3374 327 168 102 62 23
EagI C'GGCC_G
Cuts at: 0 8366 12072
Size: 8366 3706
EarI CTCTTCn'nnn_
Cuts at: 0 2219 3346 5043 7208 8086 8811 9152 9563
Size: 2219 1127 1697 2165 878 725 341 411
Cuts at: 9563 12008 12072
Size: 2445 64
Fragments arranged by size:
2445 2219 2165 1697 1127 878 725 411
341 64
EcoICRI GAG'CTC
Cuts at: 0 3153 12072
Size: 3153 8919
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1922 2074 2119 2243 2430 2449 2902 2972
Size: 1922 152 45 124 187 19 453 70
Cuts at: 2972 2989 3497 4351 4564 4889 5024 5033 5295
Size: 17 508 854 213 325 135 9 262
Cuts at: 5295 6266 6494 7009 7510 7535 8267 8480 8704
Size: 971 228 515 501 25 732 213 224
Cuts at: 8704 9748 10127 10328 10444 11407 11594 12072
Size: 1044 379 201 116 963 187 478
Fragments arranged by size:
1922 1044 971 963 854 732 515 508
501 478 453 379 325 262 228 224
213 213 201 187 187 152 135 124
116 70 45 25 19 17 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2158 3014 5726 11886 12072
Size: 2158 856 2712 6160 186
Fragments arranged by size:
6160 2712 2158 856 186
EcoO109I rG'GnC_Cy
Cuts at: 0 1119 5007 6277 6633 8208 10402 12072
Size: 1119 3888 1270 356 1575 2194 1670
Fragments arranged by size:
3888 2194 1670 1575 1270 1119 356
EcoRI G'AATT_C
Cuts at: 0 5338 10720 12072
Size: 5338 5382 1352
Fragments arranged by size:
5382 5338 1352
EcoRV GAT'ATC
Cuts at: 0 888 1272 8721 8823 11145 12072
Size: 888 384 7449 102 2322 927
Fragments arranged by size:
7449 2322 927 888 384 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1509 4356 6516 8485 8569 9355 9411 9798
Size: 1509 2847 2160 1969 84 786 56 387
Cuts at: 9798 9858 12072
Size: 60 2214
Fragments arranged by size:
2847 2214 2160 1969 1509 786 387 84
60 56
FspI TGC'GCA
Cuts at: 0 10966 12072
Size: 10966 1106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 2520 2552 2554 2586 2983 3015
Size: 403 32 2085 32 2 32 397 32
Cuts at: 3015 3232 3246 3264 3278 3597 3629 3733 3765
Size: 217 14 18 14 319 32 104 32
Cuts at: 3765 4459 4491 4788 4820 4969 5001 5496 5528
Size: 694 32 297 32 149 32 495 32
Cuts at: 5528 5677 5709 5886 5918 6600 6632 7251 7283
Size: 149 32 177 32 682 32 619 32
Cuts at: 7283 7882 7914 8827 8859 8958 8961 8990 8993
Size: 599 32 913 32 99 3 29 3
Cuts at: 8993 10649 10663 10668 10681 10695 10700 12072
Size: 1656 14 5 13 14 5 1372
Fragments arranged by size:
2085 1656 1372 913 694 682 619 599
495 403 397 319 297 217 177 149
149 104 99 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 29 18 14 14 14
14 13 5 5 3 3 2
HincII GTy'rAC
Cuts at: 0 1929 7479 8598 10532 12061 12072
Size: 1929 5550 1119 1934 1529 11
Fragments arranged by size:
5550 1934 1929 1529 1119 11
HindIII A'AGCT_T
Cuts at: 0 7605 8113 12072
Size: 7605 508 3959
Fragments arranged by size:
7605 3959 508
HpaI GTT'AAC
Cuts at: 0 7479 12072
Size: 7479 4593
KpnI G_GTAC'C
Cuts at: 0 2154 4366 8858 11319 12072
Size: 2154 2212 4492 2461 753
Fragments arranged by size:
4492 2461 2212 2154 753
MfeI C'AATT_G
Cuts at: 0 1039 4725 9209 12072
Size: 1039 3686 4484 2863
Fragments arranged by size:
4484 3686 2863 1039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 2165 2798 4052 4803 7005 7805 12072
Size: 294 1871 633 1254 751 2202 800 4267
Fragments arranged by size:
4267 2202 1871 1254 800 751 633 294
MscI TGG'CCA
Cuts at: 0 8536 11972 12072
Size: 8536 3436 100
Fragments arranged by size:
8536 3436 100
MslI CAynn'nnrTG
Cuts at: 0 188 812 946 1553 2524 3271 3667 6604
Size: 188 624 134 607 971 747 396 2937
Cuts at: 6604 8261 8395 9598 10411 12054 12072
Size: 1657 134 1203 813 1643 18
Fragments arranged by size:
2937 1657 1643 1203 971 813 747 624
607 396 188 134 134 18
MspA1I CmG'CkG
Cuts at: 0 69 132 424 1113 2148 3034 3988 4473
Size: 69 63 292 689 1035 886 954 485
Cuts at: 4473 7329 8365 8458 8697 10703 11172 12072
Size: 2856 1036 93 239 2006 469 900
Fragments arranged by size:
2856 2006 1036 1035 954 900 886 689
485 469 292 239 93 69 63
NcoI C'CATG_G
Cuts at: 0 507 4952 5782 8480 10319 12072
Size: 507 4445 830 2698 1839 1753
Fragments arranged by size:
4445 2698 1839 1753 830 507
NdeI CA'TA_TG
Cuts at: 0 2454 8406 12072
Size: 2454 5952 3666
Fragments arranged by size:
5952 3666 2454
NheI G'CTAG_C
Cuts at: 0 2683 7855 12072
Size: 2683 5172 4217
Fragments arranged by size:
5172 4217 2683
NsiI A_TGCA'T
Cuts at: 0 918 2460 7443 8137 12072
Size: 918 1542 4983 694 3935
Fragments arranged by size:
4983 3935 1542 918 694
NspI r_CATG'y
Cuts at: 0 3088 3638 4271 5268 6321 6723 7380 7411
Size: 3088 550 633 997 1053 402 657 31
Cuts at: 7411 7599 9188 10860 12072
Size: 188 1589 1672 1212
Fragments arranged by size:
3088 1672 1589 1212 1053 997 657 633
550 402 188 31
PasI CC'CwG_GG
Cuts at: 0 4294 8859 12072
Size: 4294 4565 3213
Fragments arranged by size:
4565 4294 3213
PciI A'CATG_T
Cuts at: 0 3084 3634 4267 5264 6719 7376 7595 9184
Size: 3084 550 633 997 1455 657 219 1589
Cuts at: 9184 10856 12072
Size: 1672 1216
Fragments arranged by size:
3084 1672 1589 1455 1216 997 657 633
550 219
PflMI CCAn_nnn'nTGG
Cuts at: 0 2558 6289 7496 10484 11364 12072
Size: 2558 3731 1207 2988 880 708
Fragments arranged by size:
3731 2988 2558 1207 880 708
PfoI T'CCnGG_A
Cuts at: 0 6979 10305 11800 12072
Size: 6979 3326 1495 272
Fragments arranged by size:
6979 3326 1495 272
PmlI CAC'GTG
Cuts at: 0 2957 12072
Size: 2957 9115
PpuMI rG'GwC_Cy
Cuts at: 0 1119 5007 6277 6633 12072
Size: 1119 3888 1270 356 5439
Fragments arranged by size:
5439 3888 1270 1119 356
PshAI GACnn'nnGTC
Cuts at: 0 4428 12072
Size: 4428 7644
PsiI TTA'TAA
Cuts at: 0 4282 6337 6416 10201 12072
Size: 4282 2055 79 3785 1871
Fragments arranged by size:
4282 3785 2055 1871 79
PspOMI G'GGCC_C
Cuts at: 0 8208 10402 12072
Size: 8208 2194 1670
Fragments arranged by size:
8208 2194 1670
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5643 5675 9134 9166 12072
Size: 5643 32 3459 32 2906
Fragments arranged by size:
5643 3459 2906 32 32
PstI C_TGCA'G
Cuts at: 0 1118 2199 8249 8457 10601 12072
Size: 1118 1081 6050 208 2144 1471
Fragments arranged by size:
6050 2144 1471 1118 1081 208
PvuII CAG'CTG
Cuts at: 0 69 132 424 1113 3034 3988 4473 8458
Size: 69 63 292 689 1921 954 485 3985
Cuts at: 8458 8697 10703 11172 12072
Size: 239 2006 469 900
Fragments arranged by size:
3985 2006 1921 954 900 689 485 469
292 239 69 63
SacI G_AGCT'C
Cuts at: 0 3155 12072
Size: 3155 8917
SapI GCTCTTCn'nnn_
Cuts at: 0 3346 12072
Size: 3346 8726
ScaI AGT'ACT
Cuts at: 0 378 4166 10595 12072
Size: 378 3788 6429 1477
Fragments arranged by size:
6429 3788 1477 378
SexAI A'CCwGG_T
Cuts at: 0 8591 9694 12072
Size: 8591 1103 2378
Fragments arranged by size:
8591 2378 1103
SfcI C'TryA_G
Cuts at: 0 467 1114 2195 2337 4031 6029 6846 7622
Size: 467 647 1081 142 1694 1998 817 776
Cuts at: 7622 7901 8245 8360 8453 8772 10597 11450 12072
Size: 279 344 115 93 319 1825 853 622
Fragments arranged by size:
1998 1825 1694 1081 853 817 776 647
622 467 344 319 279 142 115 93
SmlI C'TyrA_G
Cuts at: 0 483 689 3238 3616 5533 7149 7799 8512
Size: 483 206 2549 378 1917 1616 650 713
Cuts at: 8512 9772 10161 10498 11028 12072
Size: 1260 389 337 530 1044
Fragments arranged by size:
2549 1917 1616 1260 1044 713 650 530
483 389 378 337 206
SnaBI TAC'GTA
Cuts at: 0 4451 12072
Size: 4451 7621
SpeI A'CTAG_T
Cuts at: 0 4846 6246 12072
Size: 4846 1400 5826
Fragments arranged by size:
5826 4846 1400
SspI AAT'ATT
Cuts at: 0 1011 2695 3178 3771 5918 6051 6333 8024
Size: 1011 1684 483 593 2147 133 282 1691
Cuts at: 8024 8277 8892 10803 11415 12072
Size: 253 615 1911 612 657
Fragments arranged by size:
2147 1911 1691 1684 1011 657 615 612
593 483 282 253 133
StuI AGG'CCT
Cuts at: 0 8645 9529 12072
Size: 8645 884 2543
Fragments arranged by size:
8645 2543 884
StyI C'CwwG_G
Cuts at: 0 507 763 1020 3895 3963 4013 4952 5782
Size: 507 256 257 2875 68 50 939 830
Cuts at: 5782 8480 8646 9524 9530 10253 10319 10398 10791
Size: 2698 166 878 6 723 66 79 393
Cuts at: 10791 12020 12072
Size: 1229 52
Fragments arranged by size:
2875 2698 1229 939 878 830 723 507
393 257 256 166 79 68 66 52
50 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2672 5286 5914 10954 12072
Size: 2672 2614 628 5040 1118
Fragments arranged by size:
5040 2672 2614 1118 628
TatI w'GTAC_w
Cuts at: 0 145 376 840 1610 3582 4164 5581 8699
Size: 145 231 464 770 1972 582 1417 3118
Cuts at: 8699 9181 9958 10593 11349 12072
Size: 482 777 635 756 723
Fragments arranged by size:
3118 1972 1417 777 770 756 723 635
582 482 464 231 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3701 3733 5696 5728 8855 8887 9212 9244
Size: 3701 32 1963 32 3127 32 325 32
Cuts at: 9244 9338 9370 10919 10951 11633 11665 12072
Size: 94 32 1549 32 682 32 407
Fragments arranged by size:
3701 3127 1963 1549 682 407 325 94
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 9058 12072
Size: 9058 3014
XbaI T'CTAG_A
Cuts at: 0 6382 6435 12072
Size: 6382 53 5637
Fragments arranged by size:
6382 5637 53
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3414 3472 5057 7815 12072
Size: 3414 58 1585 2758 4257
Fragments arranged by size:
4257 3414 2758 1585 58
XhoI C'TCGA_G
Cuts at: 0 689 12072
Size: 689 11383
XmnI GAAnn'nnTTC
Cuts at: 0 592 4653 6731 8355 9146 11557 12072
Size: 592 4061 2078 1624 791 2411 515
Fragments arranged by size:
4061 2411 2078 1624 791 592 515
ZraI GAC'GTC
Cuts at: 0 1293 12072
Size: 1293 10779
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AvaI AvrII BaeI BamHI BanI BanII
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI Hin4I HincII HindIII HpaI KpnI
MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI
NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuII SacI SapI
ScaI SexAI SfcI SmlI SnaBI SpeI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AsiSI BbeI BbvCI BcgI BsiWI BsmBI
BssHII BssSI BstAPI DraIII EciI FseI FspAI HaeII
KasI MluI NaeI NarI NgoMIV NotI NruI PacI
PmeI PpiI PspXI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SphI SrfI
SwaI XmaI