Restriction map for
pF1KA0937
(Linear) (Six-base) MAPSORT of: KIAA0937.seq Check: 8281 from: 1 to: 1857
KIAA0937.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 756 1857
Size: 756 1101
AccI GT'mk_AC
Cuts at: 0 1328 1857
Size: 1328 529
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1651 1857
Size: 769 882 206
Fragments arranged by size:
882 769 206
AhdI GACnn_n'nnGTC
Cuts at: 0 180 1857
Size: 180 1677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 676 1857
Size: 148 528 1181
Fragments arranged by size:
1181 528 148
AlwNI CAG_nnn'CTG
Cuts at: 0 703 897 1857
Size: 703 194 960
Fragments arranged by size:
960 703 194
ApaI G_GGCC'C
Cuts at: 0 632 1273 1857
Size: 632 641 584
Fragments arranged by size:
641 632 584
AvaI C'yCGr_G
Cuts at: 0 914 1574 1857
Size: 914 660 283
Fragments arranged by size:
914 660 283
BamHI G'GATC_C
Cuts at: 0 1007 1857
Size: 1007 850
BanI G'GyrC_C
Cuts at: 0 464 730 1205 1426 1678 1857
Size: 464 266 475 221 252 179
Fragments arranged by size:
475 464 266 252 221 179
BanII G_rGCy'C
Cuts at: 0 632 817 1273 1857
Size: 632 185 456 584
Fragments arranged by size:
632 584 456 185
BbeI G_GCGC'C
Cuts at: 0 468 1857
Size: 468 1389
BbsI GAAGACnn'nnnn_
Cuts at: 0 757 1426 1857
Size: 757 669 431
Fragments arranged by size:
757 669 431
Bme1580I G_kGCm'C
Cuts at: 0 632 1273 1857
Size: 632 641 584
Fragments arranged by size:
641 632 584
BmgBI CAC'GTC
Cuts at: 0 1326 1857
Size: 1326 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 217 570 635 809 903 943 1013 1857
Size: 217 353 65 174 94 40 70 844
Fragments arranged by size:
844 353 217 174 94 70 65 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1764 1857
Size: 1764 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 771 1421 1668 1857
Size: 692 79 650 247 189
Fragments arranged by size:
692 650 247 189 79
BsaAI yAC'GTr
Cuts at: 0 399 1857
Size: 399 1458
BsaHI Gr'CG_yC
Cuts at: 0 57 307 465 1857
Size: 57 250 158 1392
Fragments arranged by size:
1392 250 158 57
BsaWI w'CCGG_w
Cuts at: 0 773 850 1502 1539 1602 1857
Size: 773 77 652 37 63 255
Fragments arranged by size:
773 652 255 77 63 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 283 313 1419 1449 1857
Size: 232 30 21 30 1106 30 408
Fragments arranged by size:
1106 408 232 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 240 1857
Size: 240 1617
BseYI C'CCAG_C
Cuts at: 0 69 135 440 1857
Size: 69 66 305 1417
Fragments arranged by size:
1417 305 69 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1209 1857
Size: 1040 169 648
Fragments arranged by size:
1040 648 169
BsiEI CG_ry'CG
Cuts at: 0 17 51 1857
Size: 17 34 1806
Fragments arranged by size:
1806 34 17
BsiHKAI G_wGCw'C
Cuts at: 0 47 1857
Size: 47 1810
BsmI GAATG_Cn'
Cuts at: 0 1529 1857
Size: 1529 328
BsmBI CGTCTCn'nnnn_
Cuts at: 0 160 1857
Size: 160 1697
Bsp1286I G_dGCh'C
Cuts at: 0 47 632 817 1273 1857
Size: 47 585 185 456 584
Fragments arranged by size:
585 584 456 185 47
BspEI T'CCGG_A
Cuts at: 0 773 1502 1602 1857
Size: 773 729 100 255
Fragments arranged by size:
773 729 255 100
BspHI T'CATG_A
Cuts at: 0 1013 1857
Size: 1013 844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 756 896 1405 1857
Size: 185 571 140 509 452
Fragments arranged by size:
571 509 452 185 140
BsrDI GCAATG_nn'
Cuts at: 0 902 1857
Size: 902 955
BsrFI r'CCGG_y
Cuts at: 0 106 461 1857
Size: 106 355 1396
Fragments arranged by size:
1396 355 106
BsrGI T'GTAC_A
Cuts at: 0 1355 1857
Size: 1355 502
BssSI C'ACGA_G
Cuts at: 0 1642 1857
Size: 1642 215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 717 1236 1788 1857
Size: 717 519 552 69
Fragments arranged by size:
717 552 519 69
BstYI r'GATC_y
Cuts at: 0 1007 1857
Size: 1007 850
Bsu36I CC'TnA_GG
Cuts at: 0 506 1257 1857
Size: 506 751 600
Fragments arranged by size:
751 600 506
BtgI C'CryG_G
Cuts at: 0 17 416 485 608 809 1764 1857
Size: 17 399 69 123 201 955 93
Fragments arranged by size:
955 399 201 123 93 69 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 746 1857
Size: 746 1111
BtsI GCAGTG_nn'
Cuts at: 0 691 1393 1838 1857
Size: 691 702 445 19
Fragments arranged by size:
702 691 445 19
EaeI y'GGCC_r
Cuts at: 0 14 48 520 866 899 1320 1479 1761
Size: 14 34 472 346 33 421 159 282
Cuts at: 1761 1857
Size: 96
Fragments arranged by size:
472 421 346 282 159 96 34 33
14
EagI C'GGCC_G
Cuts at: 0 14 48 1857
Size: 14 34 1809
Fragments arranged by size:
1809 34 14
EarI CTCTTCn'nnn_
Cuts at: 0 1555 1857
Size: 1555 302
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 187 208 448 1857
Size: 187 21 240 1409
Fragments arranged by size:
1409 240 187 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 769 771 1421 1651 1668 1857
Size: 692 77 2 650 230 17 189
Fragments arranged by size:
692 650 230 189 77 17 2
EcoO109I rG'GnC_Cy
Cuts at: 0 497 629 835 936 1269 1857
Size: 497 132 206 101 333 588
Fragments arranged by size:
588 497 333 206 132 101
HaeII r_GCGC'y
Cuts at: 0 447 468 1574 1857
Size: 447 21 1106 283
Fragments arranged by size:
1106 447 283 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 235 267 283 315 417 449
Size: 166 32 37 32 16 32 102 32
Cuts at: 449 1857
Size: 1408
Fragments arranged by size:
1408 166 102 37 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 981 1857
Size: 981 876
KasI G'GCGC_C
Cuts at: 0 464 1857
Size: 464 1393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1142 1417 1857
Size: 1142 275 440
Fragments arranged by size:
1142 440 275
MscI TGG'CCA
Cuts at: 0 522 868 901 1481 1763 1857
Size: 522 346 33 580 282 94
Fragments arranged by size:
580 522 346 282 94 33
MslI CAynn'nnrTG
Cuts at: 0 322 347 1234 1857
Size: 322 25 887 623
Fragments arranged by size:
887 623 322 25
MspA1I CmG'CkG
Cuts at: 0 53 73 639 1024 1857
Size: 53 20 566 385 833
Fragments arranged by size:
833 566 385 53 20
NaeI GCC'GGC
Cuts at: 0 108 463 1857
Size: 108 355 1394
Fragments arranged by size:
1394 355 108
NarI GG'CG_CC
Cuts at: 0 465 1857
Size: 465 1392
NcoI C'CATG_G
Cuts at: 0 416 485 809 1764 1857
Size: 416 69 324 955 93
Fragments arranged by size:
955 416 324 93 69
NgoMIV G'CCGG_C
Cuts at: 0 106 461 1857
Size: 106 355 1396
Fragments arranged by size:
1396 355 106
NspI r_CATG'y
Cuts at: 0 346 663 795 1857
Size: 346 317 132 1062
Fragments arranged by size:
1062 346 317 132
PasI CC'CwG_GG
Cuts at: 0 259 532 1108 1818 1857
Size: 259 273 576 710 39
Fragments arranged by size:
710 576 273 259 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 703 1857
Size: 703 1154
PfoI T'CCnGG_A
Cuts at: 0 301 669 1857
Size: 301 368 1188
Fragments arranged by size:
1188 368 301
PpuMI rG'GwC_Cy
Cuts at: 0 497 835 936 1857
Size: 497 338 101 921
Fragments arranged by size:
921 497 338 101
PshAI GACnn'nnGTC
Cuts at: 0 152 1857
Size: 152 1705
PspOMI G'GGCC_C
Cuts at: 0 628 1269 1857
Size: 628 641 588
Fragments arranged by size:
641 628 588
PstI C_TGCA'G
Cuts at: 0 182 893 1857
Size: 182 711 964
Fragments arranged by size:
964 711 182
PvuII CAG'CTG
Cuts at: 0 639 1857
Size: 639 1218
SanDI GG'GwC_CC
Cuts at: 0 936 1857
Size: 936 921
SexAI A'CCwGG_T
Cuts at: 0 1295 1857
Size: 1295 562
SfcI C'TryA_G
Cuts at: 0 63 178 634 889 1192 1512 1857
Size: 63 115 456 255 303 320 345
Fragments arranged by size:
456 345 320 303 255 115 63
SfoI GGC'GCC
Cuts at: 0 466 1857
Size: 466 1391
SgrAI Cr'CCGG_yG
Cuts at: 0 461 1857
Size: 461 1396
SmaI CCC'GGG
Cuts at: 0 916 1857
Size: 916 941
SnaBI TAC'GTA
Cuts at: 0 399 1857
Size: 399 1458
SphI G_CATG'C
Cuts at: 0 346 795 1857
Size: 346 449 1062
Fragments arranged by size:
1062 449 346
StyI C'CwwG_G
Cuts at: 0 416 485 809 989 1764 1857
Size: 416 69 324 180 775 93
Fragments arranged by size:
775 416 324 180 93 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1085 1223 1444 1499 1857
Size: 1085 138 221 55 358
Fragments arranged by size:
1085 358 221 138 55
TatI w'GTAC_w
Cuts at: 0 1355 1857
Size: 1355 502
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1419 1451 1857
Size: 1419 32 406
Fragments arranged by size:
1419 406 32
Tth111I GACn'n_nGTC
Cuts at: 0 1708 1857
Size: 1708 149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1474 1857
Size: 1474 383
XmaI C'CCGG_G
Cuts at: 0 914 1857
Size: 914 943
XmnI GAAnn'nnTTC
Cuts at: 0 193 1564 1857
Size: 193 1371 293
Fragments arranged by size:
1371 293 193
Enzymes that do cut:
AarI AccI AcuI AhdI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI Bme1580I BmgBI BmrI
BplI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI
EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SanDI SexAI
SfcI SfoI SgrAI SmaI SnaBI SphI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmtI Bpu10I BpuEI BsaI BsaBI BsiWI BsrBI BssHII
BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SgfI SmlI SpeI SrfI
SspI StuI SwaI XbaI XhoI ZraI