Restriction map for
pF1KSDA0934
(Linear) (Six-base) MAPSORT of: KIAA0934.seq Check: 8820 from: 1 to: 4668
KIAA0934.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2761 4619 4668
Size: 2761 1858 49
Fragments arranged by size:
2761 1858 49
AatII G_ACGT'C
Cuts at: 0 272 584 1637 3314 4668
Size: 272 312 1053 1677 1354
Fragments arranged by size:
1677 1354 1053 312 272
AccI GT'mk_AC
Cuts at: 0 2465 2604 3098 3953 4668
Size: 2465 139 494 855 715
Fragments arranged by size:
2465 855 715 494 139
Acc65I G'GTAC_C
Cuts at: 0 4358 4668
Size: 4358 310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 3055 3399 4668
Size: 221 2834 344 1269
Fragments arranged by size:
2834 1269 344 221
AfeI AGC'GCT
Cuts at: 0 256 4668
Size: 256 4412
AflIII A'CryG_T
Cuts at: 0 1501 3683 4668
Size: 1501 2182 985
Fragments arranged by size:
2182 1501 985
AhdI GACnn_n'nnGTC
Cuts at: 0 1936 4668
Size: 1936 2732
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 46 4627 4668
Size: 46 4581 41
Fragments arranged by size:
4581 46 41
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1115 1147 1708 1740 4668
Size: 1115 32 561 32 2928
Fragments arranged by size:
2928 1115 561 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 906 1569 3937 4668
Size: 906 663 2368 731
Fragments arranged by size:
2368 906 731 663
ApaI G_GGCC'C
Cuts at: 0 900 4668
Size: 900 3768
ApaLI G'TGCA_C
Cuts at: 0 3714 4668
Size: 3714 954
ApoI r'AATT_y
Cuts at: 0 823 4437 4668
Size: 823 3614 231
Fragments arranged by size:
3614 823 231
AscI GG'CGCG_CC
Cuts at: 0 49 4668
Size: 49 4619
AvaI C'yCGr_G
Cuts at: 0 1439 2016 3773 4668
Size: 1439 577 1757 895
Fragments arranged by size:
1757 1439 895 577
BamHI G'GATC_C
Cuts at: 0 870 1730 2675 4006 4668
Size: 870 860 945 1331 662
Fragments arranged by size:
1331 945 870 860 662
BanI G'GyrC_C
Cuts at: 0 445 982 999 1242 2637 3572 3596 4358
Size: 445 537 17 243 1395 935 24 762
Cuts at: 4358 4668
Size: 310
Fragments arranged by size:
1395 935 762 537 445 310 243 24
17
BanII G_rGCy'C
Cuts at: 0 192 722 900 1643 2295 2716 3733 3972
Size: 192 530 178 743 652 421 1017 239
Cuts at: 3972 4277 4398 4668
Size: 305 121 270
Fragments arranged by size:
1017 743 652 530 421 305 270 239
192 178 121
BbeI G_GCGC'C
Cuts at: 0 1003 4668
Size: 1003 3665
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 1902 2352 4668
Size: 227 1675 450 2316
Fragments arranged by size:
2316 1675 450 227
BbvCI CC'TCA_GC
Cuts at: 0 2165 4668
Size: 2165 2503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1767 1801 3186 3220 3870 3900 3904 3934
Size: 1767 34 1385 34 650 30 4 30
Cuts at: 3934 4668
Size: 734
Fragments arranged by size:
1767 1385 734 650 34 34 30 30
4
BciVI GTATCCnnnnn_n'
Cuts at: 0 3330 4668
Size: 3330 1338
BclI T'GATC_A
Cuts at: 0 1685 4668
Size: 1685 2983
BfrBI ATG'CAT
Cuts at: 0 1680 1788 1897 2040 4668
Size: 1680 108 109 143 2628
Fragments arranged by size:
2628 1680 143 109 108
BglI GCCn_nnn'nGGC
Cuts at: 0 20 1189 2243 4668
Size: 20 1169 1054 2425
Fragments arranged by size:
2425 1169 1054 20
BglII A'GATC_T
Cuts at: 0 3371 4668
Size: 3371 1297
BlpI GC'TnA_GC
Cuts at: 0 2531 4668
Size: 2531 2137
Bme1580I G_kGCm'C
Cuts at: 0 448 900 985 1235 2224 2746 3599 3718
Size: 448 452 85 250 989 522 853 119
Cuts at: 3718 4137 4239 4326 4668
Size: 419 102 87 342
Fragments arranged by size:
989 853 522 452 448 419 342 250
119 102 87 85
BmgBI CAC'GTC
Cuts at: 0 518 1502 2225 3320 4668
Size: 518 984 723 1095 1348
Fragments arranged by size:
1348 1095 984 723 518
BmrI ACTGGGnnnn_n'
Cuts at: 0 3440 3565 4668
Size: 3440 125 1103
Fragments arranged by size:
3440 1103 125
BmtI G_CTAG'C
Cuts at: 0 3442 4668
Size: 3442 1226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 58 85 91 374 441 979 1160
Size: 37 21 27 6 283 67 538 181
Cuts at: 1160 2147 2728 4165 4540 4668
Size: 987 581 1437 375 128
Fragments arranged by size:
1437 987 581 538 375 283 181 128
67 37 27 21 6
Bpu10I CC'TnA_GC
Cuts at: 0 2165 2771 4668
Size: 2165 606 1897
Fragments arranged by size:
2165 1897 606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3733 4185 4668
Size: 3733 452 483
Fragments arranged by size:
3733 483 452
BsaI GGTCTCn'nnnn_
Cuts at: 0 660 721 2835 2858 4372 4668
Size: 660 61 2114 23 1514 296
Fragments arranged by size:
2114 1514 660 296 61 23
BsaAI yAC'GTr
Cuts at: 0 635 3087 3957 4314 4668
Size: 635 2452 870 357 354
Fragments arranged by size:
2452 870 635 357 354
BsaBI GATnn'nnATC
Cuts at: 0 1690 2521 4668
Size: 1690 831 2147
Fragments arranged by size:
2147 1690 831
BsaHI Gr'CG_yC
Cuts at: 0 269 377 581 1000 1328 1634 3311 4568
Size: 269 108 204 419 328 306 1677 1257
Cuts at: 4568 4668
Size: 100
Fragments arranged by size:
1677 1257 419 328 306 269 204 108
100
BsaWI w'CCGG_w
Cuts at: 0 867 1985 4668
Size: 867 1118 2683
Fragments arranged by size:
2683 1118 867
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 731 761 1133 1163 1295 1325 1708 1738
Size: 731 30 372 30 132 30 383 30
Cuts at: 1738 2342 2372 4535 4565 4668
Size: 604 30 2163 30 103
Fragments arranged by size:
2163 731 604 383 372 132 103 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 519 1824 4537 4668
Size: 519 1305 2713 131
Fragments arranged by size:
2713 1305 519 131
BseYI C'CCAG_C
Cuts at: 0 927 1521 2331 3726 4095 4668
Size: 927 594 810 1395 369 573
Fragments arranged by size:
1395 927 810 594 573 369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 448 599 2122 2193 2228 2488 2704
Size: 382 66 151 1523 71 35 260 216
Cuts at: 2704 3004 3034 4668
Size: 300 30 1634
Fragments arranged by size:
1634 1523 382 300 260 216 151 71
66 35 30
BsiEI CG_ry'CG
Cuts at: 0 802 4668
Size: 802 3866
BsiHKAI G_wGCw'C
Cuts at: 0 485 650 722 2096 2940 3718 4277 4529
Size: 485 165 72 1374 844 778 559 252
Cuts at: 4529 4668
Size: 139
Fragments arranged by size:
1374 844 778 559 485 252 165 139
72
BsmI GAATG_Cn'
Cuts at: 0 1094 1850 4668
Size: 1094 756 2818
Fragments arranged by size:
2818 1094 756
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4163 4610 4668
Size: 4163 447 58
Fragments arranged by size:
4163 447 58
Bsp1286I G_dGCh'C
Cuts at: 0 192 448 485 650 722 900 985 1235
Size: 192 256 37 165 72 178 85 250
Cuts at: 1235 1643 2096 2224 2295 2716 2746 2940 3599
Size: 408 453 128 71 421 30 194 659
Cuts at: 3599 3718 3733 3972 4137 4239 4277 4326 4398
Size: 119 15 239 165 102 38 49 72
Cuts at: 4398 4529 4668
Size: 131 139
Fragments arranged by size:
659 453 421 408 256 250 239 194
192 178 165 165 139 131 128 119
102 85 72 72 71 49 38 37
30 15
BspEI T'CCGG_A
Cuts at: 0 1985 4668
Size: 1985 2683
BspHI T'CATG_A
Cuts at: 0 1667 4668
Size: 1667 3001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 338 2761 3016 3793 4619 4668
Size: 338 2423 255 777 826 49
Fragments arranged by size:
2423 826 777 338 255 49
BsrBI CCG'CTC
Cuts at: 0 315 548 4668
Size: 315 233 4120
Fragments arranged by size:
4120 315 233
BsrDI GCAATG_nn'
Cuts at: 0 2736 4668
Size: 2736 1932
BssHII G'CGCG_C
Cuts at: 0 49 4668
Size: 49 4619
BssSI C'ACGA_G
Cuts at: 0 245 2116 4668
Size: 245 1871 2552
Fragments arranged by size:
2552 1871 245
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3383 4605 4668
Size: 3383 1222 63
Fragments arranged by size:
3383 1222 63
BstEII G'GTnAC_C
Cuts at: 0 2493 4503 4668
Size: 2493 2010 165
Fragments arranged by size:
2493 2010 165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2439 2857 3537 3726 4653 4668
Size: 2439 418 680 189 927 15
Fragments arranged by size:
2439 927 680 418 189 15
BstYI r'GATC_y
Cuts at: 0 870 1367 1730 2675 3371 4006 4668
Size: 870 497 363 945 696 635 662
Fragments arranged by size:
945 870 696 662 635 497 363
BstZ17I GTA'TAC
Cuts at: 0 2605 4668
Size: 2605 2063
Bsu36I CC'TnA_GG
Cuts at: 0 3930 4668
Size: 3930 738
BtgI C'CryG_G
Cuts at: 0 504 680 1016 1127 1994 2549 3868 4337
Size: 504 176 336 111 867 555 1319 469
Cuts at: 4337 4587 4668
Size: 250 81
Fragments arranged by size:
1319 867 555 504 469 336 250 176
111 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1907 3177 4668
Size: 1907 1270 1491
Fragments arranged by size:
1907 1491 1270
BtsI GCAGTG_nn'
Cuts at: 0 332 967 1478 1521 1557 2432 2935 3505
Size: 332 635 511 43 36 875 503 570
Cuts at: 3505 3588 4668
Size: 83 1080
Fragments arranged by size:
1080 875 635 570 511 503 332 83
43 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4569 4604 4668
Size: 4569 35 64
Fragments arranged by size:
4569 64 35
DraI TTT'AAA
Cuts at: 0 1380 4668
Size: 1380 3288
DrdI GACnn_nn'nnGTC
Cuts at: 0 2223 4567 4668
Size: 2223 2344 101
Fragments arranged by size:
2344 2223 101
EaeI y'GGCC_r
Cuts at: 0 56 299 908 946 1214 1387 1979 2429
Size: 56 243 609 38 268 173 592 450
Cuts at: 2429 2441 3527 4668
Size: 12 1086 1141
Fragments arranged by size:
1141 1086 609 592 450 268 243 173
56 38 12
EarI CTCTTCn'nnn_
Cuts at: 0 109 405 2072 2549 2833 3797 4668
Size: 109 296 1667 477 284 964 871
Fragments arranged by size:
1667 964 871 477 296 284 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 198 592 1873 3814 4668
Size: 19 179 394 1281 1941 854
Fragments arranged by size:
1941 1281 854 394 179 19
EcoICRI GAG'CTC
Cuts at: 0 720 4275 4668
Size: 720 3555 393
Fragments arranged by size:
3555 720 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 58 85 91 221 374 441 979
Size: 37 21 27 6 130 153 67 538
Cuts at: 979 1160 2147 2728 3055 3399 4165 4540 4668
Size: 181 987 581 327 344 766 375 128
Fragments arranged by size:
987 766 581 538 375 344 327 181
153 130 128 67 37 27 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1573 1930 2917 4668
Size: 1573 357 987 1751
Fragments arranged by size:
1751 1573 987 357
EcoO109I rG'GnC_Cy
Cuts at: 0 350 686 896 1566 1968 2344 3782 3852
Size: 350 336 210 670 402 376 1438 70
Cuts at: 3852 3934 4668
Size: 82 734
Fragments arranged by size:
1438 734 670 402 376 350 336 210
82 70
EcoRI G'AATT_C
Cuts at: 0 823 4668
Size: 823 3845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2277 4668
Size: 2277 2391
HaeII r_GCGC'y
Cuts at: 0 31 258 700 1003 4299 4668
Size: 31 227 442 303 3296 369
Fragments arranged by size:
3296 442 369 303 227 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 1922 1954 2071 2103 2342 2374
Size: 406 32 1484 32 117 32 239 32
Cuts at: 2374 3551 3583 4535 4567 4668
Size: 1177 32 952 32 101
Fragments arranged by size:
1484 1177 952 406 239 117 101 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 167 4668
Size: 167 4501
KasI G'GCGC_C
Cuts at: 0 999 4668
Size: 999 3669
KpnI G_GTAC'C
Cuts at: 0 4362 4668
Size: 4362 306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 2595 4451 4521 4668
Size: 59 2536 1856 70 147
Fragments arranged by size:
2536 1856 147 70 59
MscI TGG'CCA
Cuts at: 0 301 910 1389 1981 2431 3529 4668
Size: 301 609 479 592 450 1098 1139
Fragments arranged by size:
1139 1098 609 592 479 450 301
MslI CAynn'nnrTG
Cuts at: 0 475 1240 1672 2065 4651 4668
Size: 475 765 432 393 2586 17
Fragments arranged by size:
2586 765 475 432 393 17
MspA1I CmG'CkG
Cuts at: 0 557 927 2169 2399 3018 3264 3870 4095
Size: 557 370 1242 230 619 246 606 225
Cuts at: 4095 4148 4668
Size: 53 520
Fragments arranged by size:
1242 619 606 557 520 370 246 230
225 53
NarI GG'CG_CC
Cuts at: 0 1000 4668
Size: 1000 3668
NcoI C'CATG_G
Cuts at: 0 1016 1127 4337 4668
Size: 1016 111 3210 331
Fragments arranged by size:
3210 1016 331 111
NheI G'CTAG_C
Cuts at: 0 3438 4668
Size: 3438 1230
NruI TCG'CGA
Cuts at: 0 3196 4668
Size: 3196 1472
NsiI A_TGCA'T
Cuts at: 0 1682 1790 1899 2042 4668
Size: 1682 108 109 143 2626
Fragments arranged by size:
2626 1682 143 109 108
NspI r_CATG'y
Cuts at: 0 1684 4356 4608 4668
Size: 1684 2672 252 60
Fragments arranged by size:
2672 1684 252 60
PasI CC'CwG_GG
Cuts at: 0 441 4668
Size: 441 4227
PflMI CCAn_nnn'nTGG
Cuts at: 0 573 906 4668
Size: 573 333 3762
Fragments arranged by size:
3762 573 333
PfoI T'CCnGG_A
Cuts at: 0 688 1726 2346 3332 4517 4668
Size: 688 1038 620 986 1185 151
Fragments arranged by size:
1185 1038 986 688 620 151
PmlI CAC'GTG
Cuts at: 0 3087 4668
Size: 3087 1581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1708 1740 2815 2847 4668
Size: 1708 32 1075 32 1821
Fragments arranged by size:
1821 1708 1075 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 350 686 2344 3782 3852 3934 4668
Size: 350 336 1658 1438 70 82 734
Fragments arranged by size:
1658 1438 734 350 336 82 70
PshAI GACnn'nnGTC
Cuts at: 0 1663 2870 4384 4668
Size: 1663 1207 1514 284
Fragments arranged by size:
1663 1514 1207 284
PsiI TTA'TAA
Cuts at: 0 4066 4668
Size: 4066 602
PspOMI G'GGCC_C
Cuts at: 0 896 4668
Size: 896 3772
PstI C_TGCA'G
Cuts at: 0 974 2591 3500 3583 4668
Size: 974 1617 909 83 1085
Fragments arranged by size:
1617 1085 974 909 83
PvuII CAG'CTG
Cuts at: 0 927 2169 3018 3264 4668
Size: 927 1242 849 246 1404
Fragments arranged by size:
1404 1242 927 849 246
RsrII CG'GwC_CG
Cuts at: 0 6 4668
Size: 6 4662
SacI G_AGCT'C
Cuts at: 0 722 4277 4668
Size: 722 3555 391
Fragments arranged by size:
3555 722 391
SacII CC_GC'GG
Cuts at: 0 3871 4668
Size: 3871 797
SanDI GG'GwC_CC
Cuts at: 0 686 2344 4668
Size: 686 1658 2324
Fragments arranged by size:
2324 1658 686
SexAI A'CCwGG_T
Cuts at: 0 2825 4490 4668
Size: 2825 1665 178
Fragments arranged by size:
2825 1665 178
SfcI C'TryA_G
Cuts at: 0 970 1310 2587 3496 3579 4668
Size: 970 340 1277 909 83 1089
Fragments arranged by size:
1277 1089 970 909 340 83
SfoI GGC'GCC
Cuts at: 0 1001 4668
Size: 1001 3667
SmaI CCC'GGG
Cuts at: 0 2018 4668
Size: 2018 2650
SmlI C'TyrA_G
Cuts at: 0 3748 4200 4668
Size: 3748 452 468
Fragments arranged by size:
3748 468 452
SpeI A'CTAG_T
Cuts at: 0 1321 4668
Size: 1321 3347
SphI G_CATG'C
Cuts at: 0 4356 4608 4668
Size: 4356 252 60
Fragments arranged by size:
4356 252 60
StuI AGG'CCT
Cuts at: 0 1925 2354 2541 3810 4668
Size: 1925 429 187 1269 858
Fragments arranged by size:
1925 1269 858 429 187
StyI C'CwwG_G
Cuts at: 0 995 1016 1127 2633 3092 3719 4110 4337
Size: 995 21 111 1506 459 627 391 227
Cuts at: 4337 4484 4499 4668
Size: 147 15 169
Fragments arranged by size:
1506 995 627 459 391 227 169 147
111 21 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 251 816 1513 3197 4237 4374 4379 4668
Size: 251 565 697 1684 1040 137 5 289
Fragments arranged by size:
1684 1040 697 565 289 251 137 5
TatI w'GTAC_w
Cuts at: 0 1493 1748 3668 4668
Size: 1493 255 1920 1000
Fragments arranged by size:
1920 1493 1000 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1133 1145 1165 1177 2565 2597 2888 2920
Size: 1133 12 20 12 1388 32 291 32
Cuts at: 2920 4668
Size: 1748
Fragments arranged by size:
1748 1388 1133 291 32 32 20 12
12
Tth111I GACn'n_nGTC
Cuts at: 0 3985 4668
Size: 3985 683
XbaI T'CTAG_A
Cuts at: 0 3517 4668
Size: 3517 1151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1397 3954 4515 4668
Size: 1397 2557 561 153
Fragments arranged by size:
2557 1397 561 153
XmaI C'CCGG_G
Cuts at: 0 2016 4668
Size: 2016 2652
ZraI GAC'GTC
Cuts at: 0 270 582 1635 3312 4668
Size: 270 312 1053 1677 1356
Fragments arranged by size:
1677 1356 1053 312 270
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BssHII
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII KasI KpnI MmeI MscI MslI MspA1I NarI
NcoI NheI NruI NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII
RsrII SacI SacII SanDI SexAI SfcI SfoI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AclI AflII AgeI AleI AseI AsiSI AvrII BaeI
BplI BsiWI BsrFI BsrGI BstBI ClaI DraIII EagI
EcoRV FseI FspI FspAI HincII HpaI MfeI MluI
NaeI NdeI NgoMIV NotI PacI PciI PmeI PspXI
PsrI PvuI SalI SapI SbfI ScaI SfiI SgfI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI