Restriction map for
pF1KA0930
(Linear) (Six-base) MAPSORT of: KIAA0930.seq Check: 9362 from: 1 to: 1239
KIAA0930.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 811 1239
Size: 811 428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 873 1138 1239
Size: 860 13 265 101
Fragments arranged by size:
860 265 101 13
AflIII A'CryG_T
Cuts at: 0 394 614 1239
Size: 394 220 625
Fragments arranged by size:
625 394 220
AlwNI CAG_nnn'CTG
Cuts at: 0 363 1239
Size: 363 876
ApaI G_GGCC'C
Cuts at: 0 769 1239
Size: 769 470
ApaLI G'TGCA_C
Cuts at: 0 390 1239
Size: 390 849
BanI G'GyrC_C
Cuts at: 0 957 1239
Size: 957 282
BanII G_rGCy'C
Cuts at: 0 162 643 769 1239
Size: 162 481 126 470
Fragments arranged by size:
481 470 162 126
BbsI GAAGACnn'nnnn_
Cuts at: 0 108 1239
Size: 108 1131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 242 1239
Size: 208 34 997
Fragments arranged by size:
997 208 34
BglI GCCn_nnn'nGGC
Cuts at: 0 51 703 773 793 1239
Size: 51 652 70 20 446
Fragments arranged by size:
652 446 70 51 20
BlpI GC'TnA_GC
Cuts at: 0 248 1239
Size: 248 991
Bme1580I G_kGCm'C
Cuts at: 0 394 769 962 1090 1239
Size: 394 375 193 128 149
Fragments arranged by size:
394 375 193 149 128
BmrI ACTGGGnnnn_n'
Cuts at: 0 333 452 1239
Size: 333 119 787
Fragments arranged by size:
787 333 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 1239
Size: 834 405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 931 1239
Size: 646 285 308
Fragments arranged by size:
646 308 285
BsaI GGTCTCn'nnnn_
Cuts at: 0 302 1076 1239
Size: 302 774 163
Fragments arranged by size:
774 302 163
BsaAI yAC'GTr
Cuts at: 0 397 1239
Size: 397 842
BsaWI w'CCGG_w
Cuts at: 0 968 1239
Size: 968 271
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 563 593 1239
Size: 563 30 646
Fragments arranged by size:
646 563 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 502 553 1027 1239
Size: 343 159 51 474 212
Fragments arranged by size:
474 343 212 159 51
BseYI C'CCAG_C
Cuts at: 0 855 1239
Size: 855 384
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1033 1239
Size: 248 785 206
Fragments arranged by size:
785 248 206
BsiHKAI G_wGCw'C
Cuts at: 0 394 1239
Size: 394 845
Bsp1286I G_dGCh'C
Cuts at: 0 162 394 643 769 962 1090 1239
Size: 162 232 249 126 193 128 149
Fragments arranged by size:
249 232 193 162 149 128 126
BspHI T'CATG_A
Cuts at: 0 521 1239
Size: 521 718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1073 1239
Size: 1073 166
BsrBI CCG'CTC
Cuts at: 0 876 1239
Size: 876 363
BsrFI r'CCGG_y
Cuts at: 0 275 920 1239
Size: 275 645 319
Fragments arranged by size:
645 319 275
BssSI C'ACGA_G
Cuts at: 0 871 1239
Size: 871 368
BstEII G'GTnAC_C
Cuts at: 0 375 879 1239
Size: 375 504 360
Fragments arranged by size:
504 375 360
Bsu36I CC'TnA_GG
Cuts at: 0 254 1239
Size: 254 985
BtgI C'CryG_G
Cuts at: 0 470 1239
Size: 470 769
DrdI GACnn_nn'nnGTC
Cuts at: 0 112 1239
Size: 112 1127
EaeI y'GGCC_r
Cuts at: 0 23 52 692 998 1239
Size: 23 29 640 306 241
Fragments arranged by size:
640 306 241 29 23
EarI CTCTTCn'nnn_
Cuts at: 0 836 1239
Size: 836 403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 860 873 1138 1239
Size: 834 26 13 265 101
Fragments arranged by size:
834 265 101 26 13
EcoO109I rG'GnC_Cy
Cuts at: 0 765 766 1109 1239
Size: 765 1 343 130
Fragments arranged by size:
765 343 130 1
FspI TGC'GCA
Cuts at: 0 757 1239
Size: 757 482
FspAI rTGC'GCAy
Cuts at: 0 757 1239
Size: 757 482
HaeII r_GCGC'y
Cuts at: 0 661 1239
Size: 661 578
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 563 595 1239
Size: 563 32 644
Fragments arranged by size:
644 563 32
MscI TGG'CCA
Cuts at: 0 25 1239
Size: 25 1214
MslI CAynn'nnrTG
Cuts at: 0 1231 1239
Size: 1231 8
MspA1I CmG'CkG
Cuts at: 0 247 1182 1239
Size: 247 935 57
Fragments arranged by size:
935 247 57
NcoI C'CATG_G
Cuts at: 0 470 1239
Size: 470 769
NspI r_CATG'y
Cuts at: 0 180 1239
Size: 180 1059
PasI CC'CwG_GG
Cuts at: 0 771 1239
Size: 771 468
PfoI T'CCnGG_A
Cuts at: 0 1022 1205 1239
Size: 1022 183 34
Fragments arranged by size:
1022 183 34
PmlI CAC'GTG
Cuts at: 0 397 1239
Size: 397 842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 1239
Size: 529 32 678
Fragments arranged by size:
678 529 32
PpuMI rG'GwC_Cy
Cuts at: 0 1109 1239
Size: 1109 130
PspOMI G'GGCC_C
Cuts at: 0 765 1239
Size: 765 474
PstI C_TGCA'G
Cuts at: 0 90 359 1239
Size: 90 269 880
Fragments arranged by size:
880 269 90
PvuII CAG'CTG
Cuts at: 0 247 1239
Size: 247 992
SfcI C'TryA_G
Cuts at: 0 86 355 812 1239
Size: 86 269 457 427
Fragments arranged by size:
457 427 269 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 51 1239
Size: 51 1188
SmlI C'TyrA_G
Cuts at: 0 661 946 1239
Size: 661 285 293
Fragments arranged by size:
661 293 285
StuI AGG'CCT
Cuts at: 0 302 1239
Size: 302 937
StyI C'CwwG_G
Cuts at: 0 26 470 1239
Size: 26 444 769
Fragments arranged by size:
769 444 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1064 1239
Size: 1064 175
TatI w'GTAC_w
Cuts at: 0 734 1239
Size: 734 505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 1239
Size: 144 1095
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApaLI BanI BanII
BbsI BcgI BglI BlpI Bme1580I BmrI BpmI BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstEII Bsu36I
BtgI DrdI EaeI EarI Eco57MI EcoO109I FspI FspAI
HaeII Hin4I MscI MslI MspA1I NcoI NspI PasI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SfcI
SfiI SmlI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglII BmgBI BmtI BplI Bpu10I BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BstAPI
BstBI BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI