(Linear) (Six-base) MAPSORT of: KIAA0926.seq Check: 4967 from: 1 to: 4287 KIAA0926.orf With 182 enzymes: SgfI * October 24, 2006 15:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1026 4287 Size: 1026 3261 AccI GT'mk_AC Cuts at: 0 1653 4287 Size: 1653 2634 AclI AA'CG_TT Cuts at: 0 2185 4287 Size: 2185 2102 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 82 1083 2284 2449 2791 2934 4231 Size: 67 15 1001 1201 165 342 143 1297 Cuts at: 4231 4287 Size: 56 Fragments arranged by size: 1297 1201 1001 342 165 143 67 56 15 AflIII A'CryG_T Cuts at: 0 2011 4287 Size: 2011 2276 AgeI A'CCGG_T Cuts at: 0 3156 4287 Size: 3156 1131 AhdI GACnn_n'nnGTC Cuts at: 0 498 4287 Size: 498 3789 AleI CACnn'nnGTG Cuts at: 0 343 541 3697 4287 Size: 343 198 3156 590 Fragments arranged by size: 3156 590 343 198 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1019 4287 Size: 1019 3268 AlwNI CAG_nnn'CTG Cuts at: 0 78 1194 2127 2598 2664 2694 2739 2910 Size: 78 1116 933 471 66 30 45 171 Cuts at: 2910 3405 4002 4089 4287 Size: 495 597 87 198 Fragments arranged by size: 1116 933 597 495 471 198 171 87 78 66 45 30 ApaI G_GGCC'C Cuts at: 0 1545 2919 3244 4287 Size: 1545 1374 325 1043 Fragments arranged by size: 1545 1374 1043 325 ApaLI G'TGCA_C Cuts at: 0 381 2259 3490 4287 Size: 381 1878 1231 797 Fragments arranged by size: 1878 1231 797 381 ApoI r'AATT_y Cuts at: 0 638 829 2275 3382 4287 Size: 638 191 1446 1107 905 Fragments arranged by size: 1446 1107 905 638 191 AvaI C'yCGr_G Cuts at: 0 1348 4040 4287 Size: 1348 2692 247 Fragments arranged by size: 2692 1348 247 BamHI G'GATC_C Cuts at: 0 359 559 4287 Size: 359 200 3728 Fragments arranged by size: 3728 359 200 BanII G_rGCy'C Cuts at: 0 109 394 1148 1277 1538 1545 2252 2341 Size: 109 285 754 129 261 7 707 89 Cuts at: 2341 2837 2919 3244 3794 4287 Size: 496 82 325 550 493 Fragments arranged by size: 754 707 550 496 493 325 285 261 129 109 89 82 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 145 1083 3821 4287 Size: 145 938 2738 466 Fragments arranged by size: 2738 938 466 145 BbvCI CC'TCA_GC Cuts at: 0 235 569 1727 2552 2936 4287 Size: 235 334 1158 825 384 1351 Fragments arranged by size: 1351 1158 825 384 334 235 BciVI GTATCCnnnnn_n' Cuts at: 0 960 2988 4287 Size: 960 2028 1299 Fragments arranged by size: 2028 1299 960 BclI T'GATC_A Cuts at: 0 1364 4287 Size: 1364 2923 BglI GCCn_nnn'nGGC Cuts at: 0 1174 1209 4287 Size: 1174 35 3078 Fragments arranged by size: 3078 1174 35 BglII A'GATC_T Cuts at: 0 3107 3927 4287 Size: 3107 820 360 Fragments arranged by size: 3107 820 360 BlpI GC'TnA_GC Cuts at: 0 128 1142 1682 2458 3467 4090 4287 Size: 128 1014 540 776 1009 623 197 Fragments arranged by size: 1014 1009 776 623 540 197 128 Bme1580I G_kGCm'C Cuts at: 0 385 1545 1559 2263 2919 3244 3494 4287 Size: 385 1160 14 704 656 325 250 793 Fragments arranged by size: 1160 793 704 656 385 325 250 14 BmgBI CAC'GTC Cuts at: 0 2720 3099 4287 Size: 2720 379 1188 Fragments arranged by size: 2720 1188 379 BmrI ACTGGGnnnn_n' Cuts at: 0 1113 2365 4287 Size: 1113 1252 1922 Fragments arranged by size: 1922 1252 1113 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 177 802 2519 3156 4287 Size: 177 625 1717 637 1131 Fragments arranged by size: 1717 1131 637 625 177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 462 1308 2167 2536 2660 2941 3494 Size: 84 378 846 859 369 124 281 553 Cuts at: 3494 3601 3808 3830 3941 4287 Size: 107 207 22 111 346 Fragments arranged by size: 859 846 553 378 369 346 281 207 124 111 107 84 22 Bpu10I CC'TnA_GC Cuts at: 0 235 569 1727 2552 2614 2836 2936 3074 Size: 235 334 1158 825 62 222 100 138 Cuts at: 3074 4287 Size: 1213 Fragments arranged by size: 1213 1158 825 334 235 222 138 100 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2395 3013 3176 4182 4287 Size: 2395 618 163 1006 105 Fragments arranged by size: 2395 1006 618 163 105 BsaI GGTCTCn'nnnn_ Cuts at: 0 323 1700 2512 3346 4210 4287 Size: 323 1377 812 834 864 77 Fragments arranged by size: 1377 864 834 812 323 77 BsaAI yAC'GTr Cuts at: 0 2289 4287 Size: 2289 1998 BsaHI Gr'CG_yC Cuts at: 0 435 2499 4287 Size: 435 2064 1788 Fragments arranged by size: 2064 1788 435 BsaWI w'CCGG_w Cuts at: 0 3156 4178 4287 Size: 3156 1022 109 Fragments arranged by size: 3156 1022 109 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1155 1161 1185 1191 4287 Size: 1155 6 24 6 3096 Fragments arranged by size: 3096 1155 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 70 454 928 3580 4287 Size: 70 384 474 2652 707 Fragments arranged by size: 2652 707 474 384 70 BseYI C'CCAG_C Cuts at: 0 322 546 554 574 1317 1329 1697 2764 Size: 322 224 8 20 743 12 368 1067 Cuts at: 2764 3628 4135 4287 Size: 864 507 152 Fragments arranged by size: 1067 864 743 507 368 322 224 152 20 12 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1267 1563 3988 4287 Size: 1267 296 2425 299 Fragments arranged by size: 2425 1267 299 296 BsiHKAI G_wGCw'C Cuts at: 0 109 385 1148 1277 1538 1838 2252 2263 Size: 109 276 763 129 261 300 414 11 Cuts at: 2263 2630 3494 3794 4287 Size: 367 864 300 493 Fragments arranged by size: 864 763 493 414 367 300 300 276 261 129 109 11 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2490 4287 Size: 2490 1797 Bsp1286I G_dGCh'C Cuts at: 0 109 385 394 1148 1277 1538 1545 1559 Size: 109 276 9 754 129 261 7 14 Cuts at: 1559 1838 2252 2263 2341 2630 2837 2919 3244 Size: 279 414 11 78 289 207 82 325 Cuts at: 3244 3494 3794 4287 Size: 250 300 493 Fragments arranged by size: 754 493 414 325 300 289 279 276 261 250 207 129 109 82 78 14 11 9 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1026 2792 3209 4287 Size: 1026 1766 417 1078 Fragments arranged by size: 1766 1078 1026 417 BsrBI CCG'CTC Cuts at: 0 127 1209 4287 Size: 127 1082 3078 Fragments arranged by size: 3078 1082 127 BsrDI GCAATG_nn' Cuts at: 0 1901 4287 Size: 1901 2386 BsrFI r'CCGG_y Cuts at: 0 372 1302 2075 3156 4287 Size: 372 930 773 1081 1131 Fragments arranged by size: 1131 1081 930 773 372 BssSI C'ACGA_G Cuts at: 0 4128 4287 Size: 4128 159 BstAPI GCAn_nnn'nTGC Cuts at: 0 2127 2267 4002 4287 Size: 2127 140 1735 285 Fragments arranged by size: 2127 1735 285 140 BstBI TT'CG_AA Cuts at: 0 2213 4287 Size: 2213 2074 BstEII G'GTnAC_C Cuts at: 0 2415 3366 4287 Size: 2415 951 921 Fragments arranged by size: 2415 951 921 BstXI CCAn_nnnn'nTGG Cuts at: 0 1069 4287 Size: 1069 3218 BstYI r'GATC_y Cuts at: 0 359 559 876 1190 3107 3927 4287 Size: 359 200 317 314 1917 820 360 Fragments arranged by size: 1917 820 360 359 317 314 200 Bsu36I CC'TnA_GG Cuts at: 0 1771 3728 4287 Size: 1771 1957 559 Fragments arranged by size: 1957 1771 559 BtgI C'CryG_G Cuts at: 0 697 760 2345 3245 4287 Size: 697 63 1585 900 1042 Fragments arranged by size: 1585 1042 900 697 63 BtsI GCAGTG_nn' Cuts at: 0 350 548 1312 2070 2110 2157 2477 4287 Size: 350 198 764 758 40 47 320 1810 Fragments arranged by size: 1810 764 758 350 320 198 47 40 DraI TTT'AAA Cuts at: 0 3561 4287 Size: 3561 726 DraIII CAC_nnn'GTG Cuts at: 0 3507 4011 4287 Size: 3507 504 276 Fragments arranged by size: 3507 504 276 EaeI y'GGCC_r Cuts at: 0 1028 3843 4287 Size: 1028 2815 444 Fragments arranged by size: 2815 1028 444 EarI CTCTTCn'nnn_ Cuts at: 0 114 482 1222 2404 2470 4287 Size: 114 368 740 1182 66 1817 Fragments arranged by size: 1817 1182 740 368 114 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 23 1321 4287 Size: 23 1298 2966 Fragments arranged by size: 2966 1298 23 EcoICRI GAG'CTC Cuts at: 0 107 1146 1275 1536 2250 3792 4287 Size: 107 1039 129 261 714 1542 495 Fragments arranged by size: 1542 1039 714 495 261 129 107 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 82 84 462 1083 1308 2167 2284 Size: 67 15 2 378 621 225 859 117 Cuts at: 2284 2449 2536 2660 2791 2934 2941 3494 3601 Size: 165 87 124 131 143 7 553 107 Cuts at: 3601 3808 3830 3941 4231 4287 Size: 207 22 111 290 56 Fragments arranged by size: 859 621 553 378 290 225 207 165 143 131 124 117 111 107 87 67 56 22 15 7 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 49 1666 2923 4287 Size: 49 1617 1257 1364 Fragments arranged by size: 1617 1364 1257 49 EcoO109I rG'GnC_Cy Cuts at: 0 201 608 692 1431 1542 1693 2109 2367 Size: 201 407 84 739 111 151 416 258 Cuts at: 2367 2565 2736 2915 2916 2988 3198 3240 3241 Size: 198 171 179 1 72 210 42 1 Cuts at: 3241 3250 3445 4287 Size: 9 195 842 Fragments arranged by size: 842 739 416 407 258 210 201 198 195 179 171 151 111 84 72 42 9 1 1 EcoRI G'AATT_C Cuts at: 0 3382 4287 Size: 3382 905 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 111 143 484 516 616 648 1155 1187 Size: 111 32 341 32 100 32 507 32 Cuts at: 1187 1356 1368 1388 1400 1685 1699 1717 1731 Size: 169 12 20 12 285 14 18 14 Cuts at: 1731 2728 2760 3845 3877 4209 4241 4287 Size: 997 32 1085 32 332 32 46 Fragments arranged by size: 1085 997 507 341 332 285 169 111 100 46 32 32 32 32 32 32 32 20 18 14 14 12 12 HincII GTy'rAC Cuts at: 0 1022 3273 4287 Size: 1022 2251 1014 Fragments arranged by size: 2251 1022 1014 HindIII A'AGCT_T Cuts at: 0 2296 4287 Size: 2296 1991 MfeI C'AATT_G Cuts at: 0 3116 4287 Size: 3116 1171 MscI TGG'CCA Cuts at: 0 1030 3845 4287 Size: 1030 2815 442 Fragments arranged by size: 2815 1030 442 MslI CAynn'nnrTG Cuts at: 0 154 343 541 3697 4287 Size: 154 189 198 3156 590 Fragments arranged by size: 3156 590 198 189 154 MspA1I CmG'CkG Cuts at: 0 192 417 440 706 840 1065 1103 2124 Size: 192 225 23 266 134 225 38 1021 Cuts at: 2124 2304 2551 2700 2708 2940 3326 3431 3822 Size: 180 247 149 8 232 386 105 391 Cuts at: 3822 4032 4287 Size: 210 255 Fragments arranged by size: 1021 391 386 266 255 247 232 225 225 210 192 180 149 134 105 38 23 8 NaeI GCC'GGC Cuts at: 0 374 1304 2077 4287 Size: 374 930 773 2210 Fragments arranged by size: 2210 930 773 374 NcoI C'CATG_G Cuts at: 0 697 760 2345 4287 Size: 697 63 1585 1942 Fragments arranged by size: 1942 1585 697 63 NdeI CA'TA_TG Cuts at: 0 1977 4287 Size: 1977 2310 NgoMIV G'CCGG_C Cuts at: 0 372 1302 2075 4287 Size: 372 930 773 2212 Fragments arranged by size: 2212 930 773 372 NspI r_CATG'y Cuts at: 0 1090 2229 4287 Size: 1090 1139 2058 Fragments arranged by size: 2058 1139 1090 PasI CC'CwG_GG Cuts at: 0 387 1559 4287 Size: 387 1172 2728 Fragments arranged by size: 2728 1172 387 PflMI CCAn_nnn'nTGG Cuts at: 0 2739 3405 4287 Size: 2739 666 882 Fragments arranged by size: 2739 882 666 PfoI T'CCnGG_A Cuts at: 0 100 2513 3092 3578 4287 Size: 100 2413 579 486 709 Fragments arranged by size: 2413 709 579 486 100 PmlI CAC'GTG Cuts at: 0 2289 4287 Size: 2289 1998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 774 806 3776 3808 4287 Size: 774 32 2970 32 479 Fragments arranged by size: 2970 774 479 32 32 PpuMI rG'GwC_Cy Cuts at: 0 201 608 1431 1693 2109 2367 2565 2736 Size: 201 407 823 262 416 258 198 171 Cuts at: 2736 3198 4287 Size: 462 1089 Fragments arranged by size: 1089 823 462 416 407 262 258 201 198 171 PspOMI G'GGCC_C Cuts at: 0 1541 2915 3240 4287 Size: 1541 1374 325 1047 Fragments arranged by size: 1541 1374 1047 325 PstI C_TGCA'G Cuts at: 0 998 1108 1391 2123 2135 2472 2699 2789 Size: 998 110 283 732 12 337 227 90 Cuts at: 2789 4287 Size: 1498 Fragments arranged by size: 1498 998 732 337 283 227 110 90 12 PvuII CAG'CTG Cuts at: 0 417 840 1065 1103 2124 2304 2551 2700 Size: 417 423 225 38 1021 180 247 149 Cuts at: 2700 2708 2940 3431 3822 4032 4287 Size: 8 232 491 391 210 255 Fragments arranged by size: 1021 491 423 417 391 255 247 232 225 210 180 149 38 8 SacI G_AGCT'C Cuts at: 0 109 1148 1277 1538 2252 3794 4287 Size: 109 1039 129 261 714 1542 493 Fragments arranged by size: 1542 1039 714 493 261 129 109 SanDI GG'GwC_CC Cuts at: 0 608 1693 2109 2367 4287 Size: 608 1085 416 258 1920 Fragments arranged by size: 1920 1085 608 416 258 SapI GCTCTTCn'nnn_ Cuts at: 0 114 482 1222 4287 Size: 114 368 740 3065 Fragments arranged by size: 3065 740 368 114 SexAI A'CCwGG_T Cuts at: 0 167 4287 Size: 167 4120 SfcI C'TryA_G Cuts at: 0 291 742 994 1104 1387 1470 1854 2119 Size: 291 451 252 110 283 83 384 265 Cuts at: 2119 2131 2468 2686 2695 2785 3308 4287 Size: 12 337 218 9 90 523 979 Fragments arranged by size: 979 523 451 384 337 291 283 265 252 218 110 90 83 12 9 SmlI C'TyrA_G Cuts at: 0 2410 3028 3191 4161 4287 Size: 2410 618 163 970 126 Fragments arranged by size: 2410 970 618 163 126 SspI AAT'ATT Cuts at: 0 1465 4287 Size: 1465 2822 StuI AGG'CCT Cuts at: 0 962 3910 4287 Size: 962 2948 377 Fragments arranged by size: 2948 962 377 StyI C'CwwG_G Cuts at: 0 697 760 1124 2345 3516 3641 3911 4287 Size: 697 63 364 1221 1171 125 270 376 Fragments arranged by size: 1221 1171 697 376 364 270 125 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 400 534 600 773 1551 2617 3397 4287 Size: 400 134 66 173 778 1066 780 890 Fragments arranged by size: 1066 890 780 778 400 173 134 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1161 1193 2952 2984 3154 3186 4168 4200 Size: 1161 32 1759 32 170 32 982 32 Cuts at: 4200 4287 Size: 87 Fragments arranged by size: 1759 1161 982 170 87 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 543 3252 3428 4287 Size: 543 2709 176 859 Fragments arranged by size: 2709 859 543 176 XmnI GAAnn'nnTTC Cuts at: 0 67 4153 4287 Size: 67 4086 134 Fragments arranged by size: 4086 134 67 Enzymes that do cut: AarI AccI AclI AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanII BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII MfeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI SexAI SfcI SmlI SspI StuI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AloI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BfrBI BmtI BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrGI BssHII BstZ17I BtgZI ClaI CspCI DrdI EagI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MluI MmeI NarI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI Tth111I XbaI XhoI XmaI ZraI