Restriction map for pF1KA0926
 (Linear) (Six-base) MAPSORT of: KIAA0926.seq  Check: 4967  from: 1  to: 4287



KIAA0926.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1026    4287 
   Size:       1026    3261
 
AccI GT'mk_AC

Cuts at:      0    1653    4287 
   Size:       1653    2634
 
AclI AA'CG_TT

Cuts at:      0    2185    4287 
   Size:       2185    2102
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67      82    1083    2284    2449    2791    2934    4231 
   Size:         67      15    1001    1201     165     342     143    1297

Cuts at:   4231    4287 
   Size:         56

  Fragments arranged by size: 

                1297    1201    1001     342     165     143      67      56
                  15
 
AflIII A'CryG_T

Cuts at:      0    2011    4287 
   Size:       2011    2276
 
AgeI A'CCGG_T

Cuts at:      0    3156    4287 
   Size:       3156    1131
 
AhdI GACnn_n'nnGTC

Cuts at:      0     498    4287 
   Size:        498    3789
 
AleI CACnn'nnGTG

Cuts at:      0     343     541    3697    4287 
   Size:        343     198    3156     590

  Fragments arranged by size: 

                3156     590     343     198
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1019    4287 
   Size:       1019    3268
 
AlwNI CAG_nnn'CTG

Cuts at:      0      78    1194    2127    2598    2664    2694    2739    2910 
   Size:         78    1116     933     471      66      30      45     171

Cuts at:   2910    3405    4002    4089    4287 
   Size:        495     597      87     198

  Fragments arranged by size: 

                1116     933     597     495     471     198     171      87
                  78      66      45      30
 
ApaI G_GGCC'C

Cuts at:      0    1545    2919    3244    4287 
   Size:       1545    1374     325    1043

  Fragments arranged by size: 

                1545    1374    1043     325
 
ApaLI G'TGCA_C

Cuts at:      0     381    2259    3490    4287 
   Size:        381    1878    1231     797

  Fragments arranged by size: 

                1878    1231     797     381
 
ApoI r'AATT_y

Cuts at:      0     638     829    2275    3382    4287 
   Size:        638     191    1446    1107     905

  Fragments arranged by size: 

                1446    1107     905     638     191
 
AvaI C'yCGr_G

Cuts at:      0    1348    4040    4287 
   Size:       1348    2692     247

  Fragments arranged by size: 

                2692    1348     247
 
BamHI G'GATC_C

Cuts at:      0     359     559    4287 
   Size:        359     200    3728

  Fragments arranged by size: 

                3728     359     200
 
BanII G_rGCy'C

Cuts at:      0     109     394    1148    1277    1538    1545    2252    2341 
   Size:        109     285     754     129     261       7     707      89

Cuts at:   2341    2837    2919    3244    3794    4287 
   Size:        496      82     325     550     493

  Fragments arranged by size: 

                 754     707     550     496     493     325     285     261
                 129     109      89      82       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     145    1083    3821    4287 
   Size:        145     938    2738     466

  Fragments arranged by size: 

                2738     938     466     145
 
BbvCI CC'TCA_GC

Cuts at:      0     235     569    1727    2552    2936    4287 
   Size:        235     334    1158     825     384    1351

  Fragments arranged by size: 

                1351    1158     825     384     334     235
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     960    2988    4287 
   Size:        960    2028    1299

  Fragments arranged by size: 

                2028    1299     960
 
BclI T'GATC_A

Cuts at:      0    1364    4287 
   Size:       1364    2923
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1174    1209    4287 
   Size:       1174      35    3078

  Fragments arranged by size: 

                3078    1174      35
 
BglII A'GATC_T

Cuts at:      0    3107    3927    4287 
   Size:       3107     820     360

  Fragments arranged by size: 

                3107     820     360
 
BlpI GC'TnA_GC

Cuts at:      0     128    1142    1682    2458    3467    4090    4287 
   Size:        128    1014     540     776    1009     623     197

  Fragments arranged by size: 

                1014    1009     776     623     540     197     128
 
Bme1580I G_kGCm'C

Cuts at:      0     385    1545    1559    2263    2919    3244    3494    4287 
   Size:        385    1160      14     704     656     325     250     793

  Fragments arranged by size: 

                1160     793     704     656     385     325     250      14
            
 
BmgBI CAC'GTC

Cuts at:      0    2720    3099    4287 
   Size:       2720     379    1188

  Fragments arranged by size: 

                2720    1188     379
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1113    2365    4287 
   Size:       1113    1252    1922

  Fragments arranged by size: 

                1922    1252    1113
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     177     802    2519    3156    4287 
   Size:        177     625    1717     637    1131

  Fragments arranged by size: 

                1717    1131     637     625     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     462    1308    2167    2536    2660    2941    3494 
   Size:         84     378     846     859     369     124     281     553

Cuts at:   3494    3601    3808    3830    3941    4287 
   Size:        107     207      22     111     346

  Fragments arranged by size: 

                 859     846     553     378     369     346     281     207
                 124     111     107      84      22
 
Bpu10I CC'TnA_GC

Cuts at:      0     235     569    1727    2552    2614    2836    2936    3074 
   Size:        235     334    1158     825      62     222     100     138

Cuts at:   3074    4287 
   Size:       1213

  Fragments arranged by size: 

                1213    1158     825     334     235     222     138     100
                  62
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2395    3013    3176    4182    4287 
   Size:       2395     618     163    1006     105

  Fragments arranged by size: 

                2395    1006     618     163     105
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     323    1700    2512    3346    4210    4287 
   Size:        323    1377     812     834     864      77

  Fragments arranged by size: 

                1377     864     834     812     323      77
 
BsaAI yAC'GTr

Cuts at:      0    2289    4287 
   Size:       2289    1998
 
BsaHI Gr'CG_yC

Cuts at:      0     435    2499    4287 
   Size:        435    2064    1788

  Fragments arranged by size: 

                2064    1788     435
 
BsaWI w'CCGG_w

Cuts at:      0    3156    4178    4287 
   Size:       3156    1022     109

  Fragments arranged by size: 

                3156    1022     109
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1155    1161    1185    1191    4287 
   Size:       1155       6      24       6    3096

  Fragments arranged by size: 

                3096    1155      24       6       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     454     928    3580    4287 
   Size:         70     384     474    2652     707

  Fragments arranged by size: 

                2652     707     474     384      70
 
BseYI C'CCAG_C

Cuts at:      0     322     546     554     574    1317    1329    1697    2764 
   Size:        322     224       8      20     743      12     368    1067

Cuts at:   2764    3628    4135    4287 
   Size:        864     507     152

  Fragments arranged by size: 

                1067     864     743     507     368     322     224     152
                  20      12       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1267    1563    3988    4287 
   Size:       1267     296    2425     299

  Fragments arranged by size: 

                2425    1267     299     296
 
BsiHKAI G_wGCw'C

Cuts at:      0     109     385    1148    1277    1538    1838    2252    2263 
   Size:        109     276     763     129     261     300     414      11

Cuts at:   2263    2630    3494    3794    4287 
   Size:        367     864     300     493

  Fragments arranged by size: 

                 864     763     493     414     367     300     300     276
                 261     129     109      11
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2490    4287 
   Size:       2490    1797
 
Bsp1286I G_dGCh'C

Cuts at:      0     109     385     394    1148    1277    1538    1545    1559 
   Size:        109     276       9     754     129     261       7      14

Cuts at:   1559    1838    2252    2263    2341    2630    2837    2919    3244 
   Size:        279     414      11      78     289     207      82     325

Cuts at:   3244    3494    3794    4287 
   Size:        250     300     493

  Fragments arranged by size: 

                 754     493     414     325     300     289     279     276
                 261     250     207     129     109      82      78      14
                  11       9       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1026    2792    3209    4287 
   Size:       1026    1766     417    1078

  Fragments arranged by size: 

                1766    1078    1026     417
 
BsrBI CCG'CTC

Cuts at:      0     127    1209    4287 
   Size:        127    1082    3078

  Fragments arranged by size: 

                3078    1082     127
 
BsrDI GCAATG_nn'

Cuts at:      0    1901    4287 
   Size:       1901    2386
 
BsrFI r'CCGG_y

Cuts at:      0     372    1302    2075    3156    4287 
   Size:        372     930     773    1081    1131

  Fragments arranged by size: 

                1131    1081     930     773     372
 
BssSI C'ACGA_G

Cuts at:      0    4128    4287 
   Size:       4128     159
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2127    2267    4002    4287 
   Size:       2127     140    1735     285

  Fragments arranged by size: 

                2127    1735     285     140
 
BstBI TT'CG_AA

Cuts at:      0    2213    4287 
   Size:       2213    2074
 
BstEII G'GTnAC_C

Cuts at:      0    2415    3366    4287 
   Size:       2415     951     921

  Fragments arranged by size: 

                2415     951     921
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1069    4287 
   Size:       1069    3218
 
BstYI r'GATC_y

Cuts at:      0     359     559     876    1190    3107    3927    4287 
   Size:        359     200     317     314    1917     820     360

  Fragments arranged by size: 

                1917     820     360     359     317     314     200
 
Bsu36I CC'TnA_GG

Cuts at:      0    1771    3728    4287 
   Size:       1771    1957     559

  Fragments arranged by size: 

                1957    1771     559
 
BtgI C'CryG_G

Cuts at:      0     697     760    2345    3245    4287 
   Size:        697      63    1585     900    1042

  Fragments arranged by size: 

                1585    1042     900     697      63
 
BtsI GCAGTG_nn'

Cuts at:      0     350     548    1312    2070    2110    2157    2477    4287 
   Size:        350     198     764     758      40      47     320    1810

  Fragments arranged by size: 

                1810     764     758     350     320     198      47      40
            
 
DraI TTT'AAA

Cuts at:      0    3561    4287 
   Size:       3561     726
 
DraIII CAC_nnn'GTG

Cuts at:      0    3507    4011    4287 
   Size:       3507     504     276

  Fragments arranged by size: 

                3507     504     276
 
EaeI y'GGCC_r

Cuts at:      0    1028    3843    4287 
   Size:       1028    2815     444

  Fragments arranged by size: 

                2815    1028     444
 
EarI CTCTTCn'nnn_

Cuts at:      0     114     482    1222    2404    2470    4287 
   Size:        114     368     740    1182      66    1817

  Fragments arranged by size: 

                1817    1182     740     368     114      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      23    1321    4287 
   Size:         23    1298    2966

  Fragments arranged by size: 

                2966    1298      23
 
EcoICRI GAG'CTC

Cuts at:      0     107    1146    1275    1536    2250    3792    4287 
   Size:        107    1039     129     261     714    1542     495

  Fragments arranged by size: 

                1542    1039     714     495     261     129     107
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67      82      84     462    1083    1308    2167    2284 
   Size:         67      15       2     378     621     225     859     117

Cuts at:   2284    2449    2536    2660    2791    2934    2941    3494    3601 
   Size:        165      87     124     131     143       7     553     107

Cuts at:   3601    3808    3830    3941    4231    4287 
   Size:        207      22     111     290      56

  Fragments arranged by size: 

                 859     621     553     378     290     225     207     165
                 143     131     124     117     111     107      87      67
                  56      22      15       7       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      49    1666    2923    4287 
   Size:         49    1617    1257    1364

  Fragments arranged by size: 

                1617    1364    1257      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0     201     608     692    1431    1542    1693    2109    2367 
   Size:        201     407      84     739     111     151     416     258

Cuts at:   2367    2565    2736    2915    2916    2988    3198    3240    3241 
   Size:        198     171     179       1      72     210      42       1

Cuts at:   3241    3250    3445    4287 
   Size:          9     195     842

  Fragments arranged by size: 

                 842     739     416     407     258     210     201     198
                 195     179     171     151     111      84      72      42
                   9       1       1
 
EcoRI G'AATT_C

Cuts at:      0    3382    4287 
   Size:       3382     905
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     111     143     484     516     616     648    1155    1187 
   Size:        111      32     341      32     100      32     507      32

Cuts at:   1187    1356    1368    1388    1400    1685    1699    1717    1731 
   Size:        169      12      20      12     285      14      18      14

Cuts at:   1731    2728    2760    3845    3877    4209    4241    4287 
   Size:        997      32    1085      32     332      32      46

  Fragments arranged by size: 

                1085     997     507     341     332     285     169     111
                 100      46      32      32      32      32      32      32
                  32      20      18      14      14      12      12
 
HincII GTy'rAC

Cuts at:      0    1022    3273    4287 
   Size:       1022    2251    1014

  Fragments arranged by size: 

                2251    1022    1014
 
HindIII A'AGCT_T

Cuts at:      0    2296    4287 
   Size:       2296    1991
 
MfeI C'AATT_G

Cuts at:      0    3116    4287 
   Size:       3116    1171
 
MscI TGG'CCA

Cuts at:      0    1030    3845    4287 
   Size:       1030    2815     442

  Fragments arranged by size: 

                2815    1030     442
 
MslI CAynn'nnrTG

Cuts at:      0     154     343     541    3697    4287 
   Size:        154     189     198    3156     590

  Fragments arranged by size: 

                3156     590     198     189     154
 
MspA1I CmG'CkG

Cuts at:      0     192     417     440     706     840    1065    1103    2124 
   Size:        192     225      23     266     134     225      38    1021

Cuts at:   2124    2304    2551    2700    2708    2940    3326    3431    3822 
   Size:        180     247     149       8     232     386     105     391

Cuts at:   3822    4032    4287 
   Size:        210     255

  Fragments arranged by size: 

                1021     391     386     266     255     247     232     225
                 225     210     192     180     149     134     105      38
                  23       8
 
NaeI GCC'GGC

Cuts at:      0     374    1304    2077    4287 
   Size:        374     930     773    2210

  Fragments arranged by size: 

                2210     930     773     374
 
NcoI C'CATG_G

Cuts at:      0     697     760    2345    4287 
   Size:        697      63    1585    1942

  Fragments arranged by size: 

                1942    1585     697      63
 
NdeI CA'TA_TG

Cuts at:      0    1977    4287 
   Size:       1977    2310
 
NgoMIV G'CCGG_C

Cuts at:      0     372    1302    2075    4287 
   Size:        372     930     773    2212

  Fragments arranged by size: 

                2212     930     773     372
 
NspI r_CATG'y

Cuts at:      0    1090    2229    4287 
   Size:       1090    1139    2058

  Fragments arranged by size: 

                2058    1139    1090
 
PasI CC'CwG_GG

Cuts at:      0     387    1559    4287 
   Size:        387    1172    2728

  Fragments arranged by size: 

                2728    1172     387
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2739    3405    4287 
   Size:       2739     666     882

  Fragments arranged by size: 

                2739     882     666
 
PfoI T'CCnGG_A

Cuts at:      0     100    2513    3092    3578    4287 
   Size:        100    2413     579     486     709

  Fragments arranged by size: 

                2413     709     579     486     100
 
PmlI CAC'GTG

Cuts at:      0    2289    4287 
   Size:       2289    1998
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     774     806    3776    3808    4287 
   Size:        774      32    2970      32     479

  Fragments arranged by size: 

                2970     774     479      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     201     608    1431    1693    2109    2367    2565    2736 
   Size:        201     407     823     262     416     258     198     171

Cuts at:   2736    3198    4287 
   Size:        462    1089

  Fragments arranged by size: 

                1089     823     462     416     407     262     258     201
                 198     171
 
PspOMI G'GGCC_C

Cuts at:      0    1541    2915    3240    4287 
   Size:       1541    1374     325    1047

  Fragments arranged by size: 

                1541    1374    1047     325
 
PstI C_TGCA'G

Cuts at:      0     998    1108    1391    2123    2135    2472    2699    2789 
   Size:        998     110     283     732      12     337     227      90

Cuts at:   2789    4287 
   Size:       1498

  Fragments arranged by size: 

                1498     998     732     337     283     227     110      90
                  12
 
PvuII CAG'CTG

Cuts at:      0     417     840    1065    1103    2124    2304    2551    2700 
   Size:        417     423     225      38    1021     180     247     149

Cuts at:   2700    2708    2940    3431    3822    4032    4287 
   Size:          8     232     491     391     210     255

  Fragments arranged by size: 

                1021     491     423     417     391     255     247     232
                 225     210     180     149      38       8
 
SacI G_AGCT'C

Cuts at:      0     109    1148    1277    1538    2252    3794    4287 
   Size:        109    1039     129     261     714    1542     493

  Fragments arranged by size: 

                1542    1039     714     493     261     129     109
 
SanDI GG'GwC_CC

Cuts at:      0     608    1693    2109    2367    4287 
   Size:        608    1085     416     258    1920

  Fragments arranged by size: 

                1920    1085     608     416     258
 
SapI GCTCTTCn'nnn_

Cuts at:      0     114     482    1222    4287 
   Size:        114     368     740    3065

  Fragments arranged by size: 

                3065     740     368     114
 
SexAI A'CCwGG_T

Cuts at:      0     167    4287 
   Size:        167    4120
 
SfcI C'TryA_G

Cuts at:      0     291     742     994    1104    1387    1470    1854    2119 
   Size:        291     451     252     110     283      83     384     265

Cuts at:   2119    2131    2468    2686    2695    2785    3308    4287 
   Size:         12     337     218       9      90     523     979

  Fragments arranged by size: 

                 979     523     451     384     337     291     283     265
                 252     218     110      90      83      12       9
 
SmlI C'TyrA_G

Cuts at:      0    2410    3028    3191    4161    4287 
   Size:       2410     618     163     970     126

  Fragments arranged by size: 

                2410     970     618     163     126
 
SspI AAT'ATT

Cuts at:      0    1465    4287 
   Size:       1465    2822
 
StuI AGG'CCT

Cuts at:      0     962    3910    4287 
   Size:        962    2948     377

  Fragments arranged by size: 

                2948     962     377
 
StyI C'CwwG_G

Cuts at:      0     697     760    1124    2345    3516    3641    3911    4287 
   Size:        697      63     364    1221    1171     125     270     376

  Fragments arranged by size: 

                1221    1171     697     376     364     270     125      63
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     400     534     600     773    1551    2617    3397    4287 
   Size:        400     134      66     173     778    1066     780     890

  Fragments arranged by size: 

                1066     890     780     778     400     173     134      66
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1161    1193    2952    2984    3154    3186    4168    4200 
   Size:       1161      32    1759      32     170      32     982      32

Cuts at:   4200    4287 
   Size:         87

  Fragments arranged by size: 

                1759    1161     982     170      87      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     543    3252    3428    4287 
   Size:        543    2709     176     859

  Fragments arranged by size: 

                2709     859     543     176
 
XmnI GAAnn'nnTTC

Cuts at:      0      67    4153    4287 
   Size:         67    4086     134

  Fragments arranged by size: 

                4086     134      67
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI   AflIII     AgeI     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI    BanII
     BbsI    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    Hin4I   HincII  HindIII     MfeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SapI
    SexAI     SfcI     SmlI     SspI     StuI     StyI    TaqII     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII     AloI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI     BbeI     BcgI    BfrBI     BmtI    BsaBI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrGI   BssHII  BstZ17I
    BtgZI     ClaI    CspCI     DrdI     EagI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI     MmeI
     NarI     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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