Restriction map for
pF1KA0919
(Linear) (Six-base) MAPSORT of: KIAA0919.seq Check: 2211 from: 1 to: 2016
KIAA0919.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 360 894 1928 2016
Size: 360 534 1034 88
Fragments arranged by size:
1034 534 360 88
Acc65I G'GTAC_C
Cuts at: 0 846 2016
Size: 846 1170
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1219 1546 1580 1838 2016
Size: 370 849 327 34 258 178
Fragments arranged by size:
849 370 327 258 178 34
AleI CACnn'nnGTG
Cuts at: 0 1744 2016
Size: 1744 272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 488 1639 2016
Size: 488 1151 377
Fragments arranged by size:
1151 488 377
ApaI G_GGCC'C
Cuts at: 0 104 858 2016
Size: 104 754 1158
Fragments arranged by size:
1158 754 104
ApoI r'AATT_y
Cuts at: 0 809 1474 1507 1723 2016
Size: 809 665 33 216 293
Fragments arranged by size:
809 665 293 216 33
AseI AT'TA_AT
Cuts at: 0 400 2016
Size: 400 1616
AvaI C'yCGr_G
Cuts at: 0 920 2016
Size: 920 1096
BamHI G'GATC_C
Cuts at: 0 59 2016
Size: 59 1957
BanI G'GyrC_C
Cuts at: 0 846 916 1783 2016
Size: 846 70 867 233
Fragments arranged by size:
867 846 233 70
BanII G_rGCy'C
Cuts at: 0 104 858 2016
Size: 104 754 1158
Fragments arranged by size:
1158 754 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 403 830 2016
Size: 403 427 1186
Fragments arranged by size:
1186 427 403
BciVI GTATCCnnnnn_n'
Cuts at: 0 178 285 2016
Size: 178 107 1731
Fragments arranged by size:
1731 178 107
BfrBI ATG'CAT
Cuts at: 0 748 999 2016
Size: 748 251 1017
Fragments arranged by size:
1017 748 251
BglII A'GATC_T
Cuts at: 0 1539 2016
Size: 1539 477
Bme1580I G_kGCm'C
Cuts at: 0 104 858 921 2016
Size: 104 754 63 1095
Fragments arranged by size:
1095 754 104 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1637 2016
Size: 1637 379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 637 863 2016
Size: 55 582 226 1153
Fragments arranged by size:
1153 582 226 55
Bpu10I CC'TnA_GC
Cuts at: 0 109 2016
Size: 109 1907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 2016
Size: 500 1516
BsaI GGTCTCn'nnnn_
Cuts at: 0 1283 2016
Size: 1283 733
BsaBI GATnn'nnATC
Cuts at: 0 1544 1678 2016
Size: 1544 134 338
Fragments arranged by size:
1544 338 134
BsaWI w'CCGG_w
Cuts at: 0 1891 2016
Size: 1891 125
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 272 2016
Size: 272 1744
BseYI C'CCAG_C
Cuts at: 0 671 2016
Size: 671 1345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 2016
Size: 458 1558
BsiEI CG_ry'CG
Cuts at: 0 183 2016
Size: 183 1833
BsiHKAI G_wGCw'C
Cuts at: 0 53 82 2016
Size: 53 29 1934
Fragments arranged by size:
1934 53 29
Bsp1286I G_dGCh'C
Cuts at: 0 53 82 104 858 921 2016
Size: 53 29 22 754 63 1095
Fragments arranged by size:
1095 754 63 53 29 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 518 1315 2016
Size: 518 797 701
Fragments arranged by size:
797 701 518
BsrBI CCG'CTC
Cuts at: 0 6 57 2016
Size: 6 51 1959
Fragments arranged by size:
1959 51 6
BsrDI GCAATG_nn'
Cuts at: 0 344 1934 2016
Size: 344 1590 82
Fragments arranged by size:
1590 344 82
BsrGI T'GTAC_A
Cuts at: 0 428 1391 2016
Size: 428 963 625
Fragments arranged by size:
963 625 428
BssSI C'ACGA_G
Cuts at: 0 52 2016
Size: 52 1964
BstYI r'GATC_y
Cuts at: 0 59 1539 2016
Size: 59 1480 477
Fragments arranged by size:
1480 477 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1128 2016
Size: 1128 888
BtsI GCAGTG_nn'
Cuts at: 0 361 2016
Size: 361 1655
ClaI AT'CG_AT
Cuts at: 0 531 888 2016
Size: 531 357 1128
Fragments arranged by size:
1128 531 357
EarI CTCTTCn'nnn_
Cuts at: 0 338 836 1379 1817 2016
Size: 338 498 543 438 199
Fragments arranged by size:
543 498 438 338 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 40 2016
Size: 34 6 1976
Fragments arranged by size:
1976 34 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 370 637 863 1219 1546 1580 1838
Size: 55 315 267 226 356 327 34 258
Cuts at: 1838 2016
Size: 178
Fragments arranged by size:
356 327 315 267 258 226 178 55
34
EcoO109I rG'GnC_Cy
Cuts at: 0 854 2016
Size: 854 1162
EcoRI G'AATT_C
Cuts at: 0 809 2016
Size: 809 1207
HincII GTy'rAC
Cuts at: 0 361 2016
Size: 361 1655
HindIII A'AGCT_T
Cuts at: 0 154 565 2016
Size: 154 411 1451
Fragments arranged by size:
1451 411 154
KpnI G_GTAC'C
Cuts at: 0 850 2016
Size: 850 1166
MslI CAynn'nnrTG
Cuts at: 0 1744 2016
Size: 1744 272
MspA1I CmG'CkG
Cuts at: 0 43 1816 1869 2016
Size: 43 1773 53 147
Fragments arranged by size:
1773 147 53 43
NsiI A_TGCA'T
Cuts at: 0 750 1001 2016
Size: 750 251 1015
Fragments arranged by size:
1015 750 251
NspI r_CATG'y
Cuts at: 0 999 1275 1813 2016
Size: 999 276 538 203
Fragments arranged by size:
999 538 276 203
PasI CC'CwG_GG
Cuts at: 0 1083 2016
Size: 1083 933
PflMI CCAn_nnn'nTGG
Cuts at: 0 864 2016
Size: 864 1152
PspOMI G'GGCC_C
Cuts at: 0 100 854 2016
Size: 100 754 1162
Fragments arranged by size:
1162 754 100
PstI C_TGCA'G
Cuts at: 0 1628 2016
Size: 1628 388
PvuII CAG'CTG
Cuts at: 0 1816 1869 2016
Size: 1816 53 147
Fragments arranged by size:
1816 147 53
SalI G'TCGA_C
Cuts at: 0 359 2016
Size: 359 1657
SapI GCTCTTCn'nnn_
Cuts at: 0 338 2016
Size: 338 1678
ScaI AGT'ACT
Cuts at: 0 780 1714 2016
Size: 780 934 302
Fragments arranged by size:
934 780 302
SexAI A'CCwGG_T
Cuts at: 0 1922 2016
Size: 1922 94
SfcI C'TryA_G
Cuts at: 0 1017 1359 1624 1864 2016
Size: 1017 342 265 240 152
Fragments arranged by size:
1017 342 265 240 152
SmaI CCC'GGG
Cuts at: 0 922 2016
Size: 922 1094
SmlI C'TyrA_G
Cuts at: 0 515 2016
Size: 515 1501
SpeI A'CTAG_T
Cuts at: 0 781 2016
Size: 781 1235
SspI AAT'ATT
Cuts at: 0 378 1650 2016
Size: 378 1272 366
Fragments arranged by size:
1272 378 366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 197 904 2016
Size: 197 707 1112
Fragments arranged by size:
1112 707 197
TatI w'GTAC_w
Cuts at: 0 428 778 1391 1712 2016
Size: 428 350 613 321 304
Fragments arranged by size:
613 428 350 321 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1959 1991 2016
Size: 1959 32 25
Fragments arranged by size:
1959 32 25
XbaI T'CTAG_A
Cuts at: 0 495 2016
Size: 495 1521
XmaI C'CCGG_G
Cuts at: 0 920 2016
Size: 920 1096
XmnI GAAnn'nnTTC
Cuts at: 0 378 1541 2016
Size: 378 1163 475
Fragments arranged by size:
1163 475 378
Enzymes that do cut:
AccI Acc65I AcuI AleI AlfI ApaI ApoI AseI
AvaI BamHI BanI BanII BbsI BciVI BfrBI BglII
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrGI BssSI BstYI BtgZI BtsI ClaI EarI
EciI Eco57MI EcoO109I EcoRI HincII HindIII KpnI MslI
MspA1I NsiI NspI PasI PflMI PspOMI PstI PvuII
SalI SapI ScaI SexAI SfcI SmaI SmlI SpeI
SspI TaqII TatI TstI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI AscI AsiSI AvrII BaeI BbeI
BbvCI BcgI BclI BglI BlpI BmgBI BmrI BmtI
BsaAI BsaHI BsaXI BsiWI BsmI BsmBI BspEI BspHI
BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HpaI KasI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SanDI SbfI SfiI
SfoI SgfI SgrAI SnaBI SphI SrfI StuI StyI
SwaI Tth111I XcmI XhoI ZraI