Restriction map for
pF1KSDA0908
(Linear) (Six-base) MAPSORT of: KIAA0908.seq Check: 9340 from: 1 to: 1515
KIAA0908.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 887 1515
Size: 887 628
AclI AA'CG_TT
Cuts at: 0 300 1515
Size: 300 1215
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 510 1515
Size: 125 385 1005
Fragments arranged by size:
1005 385 125
AflIII A'CryG_T
Cuts at: 0 458 473 1515
Size: 458 15 1042
Fragments arranged by size:
1042 458 15
ApoI r'AATT_y
Cuts at: 0 166 1003 1090 1250 1515
Size: 166 837 87 160 265
Fragments arranged by size:
837 265 166 160 87
AvaI C'yCGr_G
Cuts at: 0 73 1515
Size: 73 1442
BanI G'GyrC_C
Cuts at: 0 79 1515
Size: 79 1436
BanII G_rGCy'C
Cuts at: 0 23 1515
Size: 23 1492
BbeI G_GCGC'C
Cuts at: 0 83 1515
Size: 83 1432
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 107 1515
Size: 73 34 1408
Fragments arranged by size:
1408 73 34
BclI T'GATC_A
Cuts at: 0 1476 1515
Size: 1476 39
BglI GCCn_nnn'nGGC
Cuts at: 0 77 1515
Size: 77 1438
BglII A'GATC_T
Cuts at: 0 1217 1515
Size: 1217 298
Bme1580I G_kGCm'C
Cuts at: 0 1160 1463 1515
Size: 1160 303 52
Fragments arranged by size:
1160 303 52
BmgBI CAC'GTC
Cuts at: 0 474 1515
Size: 474 1041
BmrI ACTGGGnnnn_n'
Cuts at: 0 361 1515
Size: 361 1154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 1515
Size: 42 1473
Bpu10I CC'TnA_GC
Cuts at: 0 774 1184 1515
Size: 774 410 331
Fragments arranged by size:
774 410 331
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 1515
Size: 325 1190
BsaHI Gr'CG_yC
Cuts at: 0 80 1515
Size: 80 1435
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 254 284 1515
Size: 254 30 1231
Fragments arranged by size:
1231 254 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 400 575 847 1515
Size: 18 382 175 272 668
Fragments arranged by size:
668 382 272 175 18
BseYI C'CCAG_C
Cuts at: 0 36 1515
Size: 36 1479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 259 274 1406 1415 1515
Size: 78 181 15 1132 9 100
Fragments arranged by size:
1132 181 100 78 15 9
BsiHKAI G_wGCw'C
Cuts at: 0 23 96 1515
Size: 23 73 1419
Fragments arranged by size:
1419 73 23
BsmI GAATG_Cn'
Cuts at: 0 584 1515
Size: 584 931
BsmBI CGTCTCn'nnnn_
Cuts at: 0 30 1515
Size: 30 1485
Bsp1286I G_dGCh'C
Cuts at: 0 23 96 1160 1463 1515
Size: 23 73 1064 303 52
Fragments arranged by size:
1064 303 73 52 23
BspHI T'CATG_A
Cuts at: 0 488 513 1515
Size: 488 25 1002
Fragments arranged by size:
1002 488 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 1515
Size: 164 1351
BsrDI GCAATG_nn'
Cuts at: 0 1412 1515
Size: 1412 103
BsrFI r'CCGG_y
Cuts at: 0 616 1515
Size: 616 899
BsrGI T'GTAC_A
Cuts at: 0 824 1515
Size: 824 691
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1393 1438 1515
Size: 1393 45 77
Fragments arranged by size:
1393 77 45
BstYI r'GATC_y
Cuts at: 0 494 1179 1217 1515
Size: 494 685 38 298
Fragments arranged by size:
685 494 298 38
BtgI C'CryG_G
Cuts at: 0 1294 1397 1515
Size: 1294 103 118
Fragments arranged by size:
1294 118 103
DraI TTT'AAA
Cuts at: 0 284 1256 1515
Size: 284 972 259
Fragments arranged by size:
972 284 259
EaeI y'GGCC_r
Cuts at: 0 675 938 1515
Size: 675 263 577
Fragments arranged by size:
675 577 263
EarI CTCTTCn'nnn_
Cuts at: 0 200 1515
Size: 200 1315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 86 1515
Size: 86 1429
EcoICRI GAG'CTC
Cuts at: 0 21 1515
Size: 21 1494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 510 1515
Size: 125 385 1005
Fragments arranged by size:
1005 385 125
EcoRI G'AATT_C
Cuts at: 0 1003 1515
Size: 1003 512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 636 1252 1515
Size: 636 616 263
Fragments arranged by size:
636 616 263
HaeII r_GCGC'y
Cuts at: 0 83 432 1515
Size: 83 349 1083
Fragments arranged by size:
1083 349 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 194 226 254 286 1515
Size: 194 32 28 32 1229
Fragments arranged by size:
1229 194 32 32 28
KasI G'GCGC_C
Cuts at: 0 79 1515
Size: 79 1436
MfeI C'AATT_G
Cuts at: 0 995 1515
Size: 995 520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1515
Size: 889 626
MscI TGG'CCA
Cuts at: 0 677 940 1515
Size: 677 263 575
Fragments arranged by size:
677 575 263
MslI CAynn'nnrTG
Cuts at: 0 235 518 1402 1468 1515
Size: 235 283 884 66 47
Fragments arranged by size:
884 283 235 66 47
MspA1I CmG'CkG
Cuts at: 0 62 1451 1515
Size: 62 1389 64
Fragments arranged by size:
1389 64 62
NaeI GCC'GGC
Cuts at: 0 618 1515
Size: 618 897
NarI GG'CG_CC
Cuts at: 0 80 1515
Size: 80 1435
NcoI C'CATG_G
Cuts at: 0 1294 1397 1515
Size: 1294 103 118
Fragments arranged by size:
1294 118 103
NdeI CA'TA_TG
Cuts at: 0 1206 1515
Size: 1206 309
NgoMIV G'CCGG_C
Cuts at: 0 616 1515
Size: 616 899
NruI TCG'CGA
Cuts at: 0 112 1515
Size: 112 1403
NspI r_CATG'y
Cuts at: 0 462 1515
Size: 462 1053
PciI A'CATG_T
Cuts at: 0 458 1515
Size: 458 1057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 891 923 1515
Size: 891 32 592
Fragments arranged by size:
891 592 32
PshAI GACnn'nnGTC
Cuts at: 0 317 1515
Size: 317 1198
PstI C_TGCA'G
Cuts at: 0 788 1515
Size: 788 727
PvuII CAG'CTG
Cuts at: 0 62 1515
Size: 62 1453
SacI G_AGCT'C
Cuts at: 0 23 1515
Size: 23 1492
SfcI C'TryA_G
Cuts at: 0 784 1515
Size: 784 731
SfoI GGC'GCC
Cuts at: 0 81 1515
Size: 81 1434
SspI AAT'ATT
Cuts at: 0 969 1015 1515
Size: 969 46 500
Fragments arranged by size:
969 500 46
StuI AGG'CCT
Cuts at: 0 337 1515
Size: 337 1178
StyI C'CwwG_G
Cuts at: 0 573 1294 1397 1515
Size: 573 721 103 118
Fragments arranged by size:
721 573 118 103
TatI w'GTAC_w
Cuts at: 0 824 851 1515
Size: 824 27 664
Fragments arranged by size:
824 664 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 846 878 1515
Size: 846 32 637
Fragments arranged by size:
846 637 32
Tth111I GACn'n_nGTC
Cuts at: 0 928 1515
Size: 928 587
XbaI T'CTAG_A
Cuts at: 0 1075 1515
Size: 1075 440
Enzymes that do cut:
AccI AclI AcuI AflIII ApoI AvaI BanI BanII
BbeI BcgI BclI BglI BglII Bme1580I BmgBI BmrI
BplI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BstAPI BstYI BtgI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoRI FalI HaeII Hin4I KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NspI PciI PpiI PshAI PstI PvuII
SacI SfcI SfoI SspI StuI StyI TatI TstI
Tth111I XbaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BfrBI BlpI
BmtI BpmI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI
BspEI BsrBI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EcoNI EcoO109I EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MluI NheI NotI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI TaqII XcmI XhoI XmaI
XmnI ZraI