Restriction map for
pF1KSDA0907
(Linear) (Six-base) MAPSORT of: KIAA0907.seq Check: 7444 from: 1 to: 1692
KIAA0907.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 241 335 1692
Size: 140 101 94 1357
Fragments arranged by size:
1357 140 101 94
AflIII A'CryG_T
Cuts at: 0 343 1692
Size: 343 1349
AlwNI CAG_nnn'CTG
Cuts at: 0 607 853 1585 1692
Size: 607 246 732 107
Fragments arranged by size:
732 607 246 107
AseI AT'TA_AT
Cuts at: 0 197 326 989 1692
Size: 197 129 663 703
Fragments arranged by size:
703 663 197 129
AvaI C'yCGr_G
Cuts at: 0 362 416 1692
Size: 362 54 1276
Fragments arranged by size:
1276 362 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 983 1016 1692
Size: 983 33 676
Fragments arranged by size:
983 676 33
BanI G'GyrC_C
Cuts at: 0 38 139 802 1692
Size: 38 101 663 890
Fragments arranged by size:
890 663 101 38
BbeI G_GCGC'C
Cuts at: 0 42 1692
Size: 42 1650
BbsI GAAGACnn'nnnn_
Cuts at: 0 804 1692
Size: 804 888
BbvCI CC'TCA_GC
Cuts at: 0 1109 1692
Size: 1109 583
BciVI GTATCCnnnnn_n'
Cuts at: 0 1426 1692
Size: 1426 266
BfrBI ATG'CAT
Cuts at: 0 684 1692
Size: 684 1008
Bme1580I G_kGCm'C
Cuts at: 0 142 1692
Size: 142 1550
BmtI G_CTAG'C
Cuts at: 0 1256 1692
Size: 1256 436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 50 629 1196 1220 1232 1464 1692
Size: 47 3 579 567 24 12 232 228
Fragments arranged by size:
579 567 232 228 47 24 12 3
Bpu10I CC'TnA_GC
Cuts at: 0 1109 1692
Size: 1109 583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 1692
Size: 359 1333
BsaHI Gr'CG_yC
Cuts at: 0 39 1692
Size: 39 1653
BsaWI w'CCGG_w
Cuts at: 0 527 1692
Size: 527 1165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 135 165 1629 1659 1692
Size: 135 30 1464 30 33
Fragments arranged by size:
1464 135 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1037 1097 1411 1692
Size: 1037 60 314 281
Fragments arranged by size:
1037 314 281 60
BseYI C'CCAG_C
Cuts at: 0 1328 1334 1340 1346 1660 1692
Size: 1328 6 6 6 314 32
Fragments arranged by size:
1328 314 32 6 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 1523 1692
Size: 1261 262 169
Fragments arranged by size:
1261 262 169
BsmI GAATG_Cn'
Cuts at: 0 246 1692
Size: 246 1446
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1377 1692
Size: 1377 315
Bsp1286I G_dGCh'C
Cuts at: 0 142 1692
Size: 142 1550
BspHI T'CATG_A
Cuts at: 0 428 1692
Size: 428 1264
BsrFI r'CCGG_y
Cuts at: 0 1185 1692
Size: 1185 507
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1560 1692
Size: 1560 132
BstEII G'GTnAC_C
Cuts at: 0 111 1087 1692
Size: 111 976 605
Fragments arranged by size:
976 605 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 555 1692
Size: 210 345 1137
Fragments arranged by size:
1137 345 210
Bsu36I CC'TnA_GG
Cuts at: 0 1527 1692
Size: 1527 165
BtgI C'CryG_G
Cuts at: 0 5 1641 1692
Size: 5 1636 51
Fragments arranged by size:
1636 51 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 615 1692
Size: 615 1077
BtsI GCAGTG_nn'
Cuts at: 0 126 1692
Size: 126 1566
EaeI y'GGCC_r
Cuts at: 0 855 1692
Size: 855 837
EarI CTCTTCn'nnn_
Cuts at: 0 85 1375 1692
Size: 85 1290 317
Fragments arranged by size:
1290 317 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 50 140 241 335 629 1196 1220
Size: 47 3 90 101 94 294 567 24
Cuts at: 1220 1232 1464 1692
Size: 12 232 228
Fragments arranged by size:
567 294 232 228 101 94 90 47
24 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 269 1007 1692
Size: 269 738 685
Fragments arranged by size:
738 685 269
EcoO109I rG'GnC_Cy
Cuts at: 0 1625 1692
Size: 1625 67
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 602 684 1692
Size: 602 82 1008
Fragments arranged by size:
1008 602 82
HaeII r_GCGC'y
Cuts at: 0 42 1692
Size: 42 1650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 835 867 1263 1295 1692
Size: 835 32 396 32 397
Fragments arranged by size:
835 397 396 32 32
HindIII A'AGCT_T
Cuts at: 0 253 863 928 1692
Size: 253 610 65 764
Fragments arranged by size:
764 610 253 65
KasI G'GCGC_C
Cuts at: 0 38 1692
Size: 38 1654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1692
Size: 607 1085
MslI CAynn'nnrTG
Cuts at: 0 208 1453 1692
Size: 208 1245 239
Fragments arranged by size:
1245 239 208
MspA1I CmG'CkG
Cuts at: 0 7 94 148 649 1159 1692
Size: 7 87 54 501 510 533
Fragments arranged by size:
533 510 501 87 54 7
NaeI GCC'GGC
Cuts at: 0 1187 1692
Size: 1187 505
NarI GG'CG_CC
Cuts at: 0 39 1692
Size: 39 1653
NcoI C'CATG_G
Cuts at: 0 1641 1692
Size: 1641 51
NdeI CA'TA_TG
Cuts at: 0 1454 1692
Size: 1454 238
NgoMIV G'CCGG_C
Cuts at: 0 1185 1692
Size: 1185 507
NheI G'CTAG_C
Cuts at: 0 1252 1692
Size: 1252 440
NsiI A_TGCA'T
Cuts at: 0 686 1692
Size: 686 1006
NspI r_CATG'y
Cuts at: 0 347 724 1692
Size: 347 377 968
Fragments arranged by size:
968 377 347
PciI A'CATG_T
Cuts at: 0 343 1692
Size: 343 1349
PflMI CCAn_nnn'nTGG
Cuts at: 0 853 1072 1585 1692
Size: 853 219 513 107
Fragments arranged by size:
853 513 219 107
PfoI T'CCnGG_A
Cuts at: 0 24 1692
Size: 24 1668
PpuMI rG'GwC_Cy
Cuts at: 0 1625 1692
Size: 1625 67
PspXI vC'TCGA_Gb
Cuts at: 0 362 416 1692
Size: 362 54 1276
Fragments arranged by size:
1276 362 54
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 710 742 1692
Size: 710 32 950
Fragments arranged by size:
950 710 32
PstI C_TGCA'G
Cuts at: 0 408 1692
Size: 408 1284
PvuII CAG'CTG
Cuts at: 0 148 649 1159 1692
Size: 148 501 510 533
Fragments arranged by size:
533 510 501 148
SacII CC_GC'GG
Cuts at: 0 8 1692
Size: 8 1684
SexAI A'CCwGG_T
Cuts at: 0 302 1692
Size: 302 1390
SfcI C'TryA_G
Cuts at: 0 404 1692
Size: 404 1288
SfoI GGC'GCC
Cuts at: 0 40 1692
Size: 40 1652
SmlI C'TyrA_G
Cuts at: 0 362 374 416 1692
Size: 362 12 42 1276
Fragments arranged by size:
1276 362 42 12
StuI AGG'CCT
Cuts at: 0 275 911 1692
Size: 275 636 781
Fragments arranged by size:
781 636 275
StyI C'CwwG_G
Cuts at: 0 164 1641 1692
Size: 164 1477 51
Fragments arranged by size:
1477 164 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 1264 1692
Size: 642 622 428
Fragments arranged by size:
642 622 428
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 135 167 1629 1661 1692
Size: 65 32 38 32 1462 32 31
Fragments arranged by size:
1462 65 38 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 1265 1692
Size: 1265 427
XbaI T'CTAG_A
Cuts at: 0 714 970 1692
Size: 714 256 722
Fragments arranged by size:
722 714 256
XhoI C'TCGA_G
Cuts at: 0 362 416 1692
Size: 362 54 1276
Fragments arranged by size:
1276 362 54
Enzymes that do cut:
AcuI AflIII AlwNI AseI AvaI BaeI BanI BbeI
BbsI BbvCI BciVI BfrBI Bme1580I BmtI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmI
BsmBI Bsp1286I BspHI BsrFI BstAPI BstEII BstXI Bsu36I
BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HindIII KasI MmeI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PpuMI PspXI PsrI PstI PvuII
SacII SexAI SfcI SfoI SmlI StuI StyI TaqII
TstI Tth111I XbaI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AsiSI AvrII BamHI BanII BcgI BclI BglI BglII
BlpI BmgBI BmrI BplI BsaI BsaAI BsaBI BsiEI
BsiHKAI BsiWI BspEI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstYI BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MscI NotI
NruI PacI PasI PmeI PmlI PpiI PshAI PsiI
PspOMI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI XcmI XmaI XmnI ZraI