Restriction map for
pF1KA0906
(Linear) (Six-base) MAPSORT of: KIAA0906.seq Check: 9362 from: 1 to: 5661
KIAA0906.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2313 3471 5028 5661
Size: 2313 1158 1557 633
Fragments arranged by size:
2313 1557 1158 633
AatII G_ACGT'C
Cuts at: 0 752 3656 5661
Size: 752 2904 2005
Fragments arranged by size:
2904 2005 752
AccI GT'mk_AC
Cuts at: 0 553 677 752 2270 5661
Size: 553 124 75 1518 3391
Fragments arranged by size:
3391 1518 553 124 75
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 430 838 1437 2388 3076 5043 5661
Size: 429 1 408 599 951 688 1967 618
Fragments arranged by size:
1967 951 688 618 599 429 408 1
AfeI AGC'GCT
Cuts at: 0 3369 5661
Size: 3369 2292
AflIII A'CryG_T
Cuts at: 0 917 1641 2291 2921 5661
Size: 917 724 650 630 2740
Fragments arranged by size:
2740 917 724 650 630
AleI CACnn'nnGTG
Cuts at: 0 1061 1279 1892 5661
Size: 1061 218 613 3769
Fragments arranged by size:
3769 1061 613 218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2371 3568 3628 5661
Size: 2371 1197 60 2033
Fragments arranged by size:
2371 2033 1197 60
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3580 3612 5661
Size: 3580 32 2049
Fragments arranged by size:
3580 2049 32
AlwNI CAG_nnn'CTG
Cuts at: 0 318 423 1492 1656 1804 2940 5661
Size: 318 105 1069 164 148 1136 2721
Fragments arranged by size:
2721 1136 1069 318 164 148 105
ApaI G_GGCC'C
Cuts at: 0 61 874 1434 4315 5375 5661
Size: 61 813 560 2881 1060 286
Fragments arranged by size:
2881 1060 813 560 286 61
ApaLI G'TGCA_C
Cuts at: 0 2836 4773 5661
Size: 2836 1937 888
Fragments arranged by size:
2836 1937 888
ApoI r'AATT_y
Cuts at: 0 1346 2029 5661
Size: 1346 683 3632
Fragments arranged by size:
3632 1346 683
AseI AT'TA_AT
Cuts at: 0 3895 5661
Size: 3895 1766
AvaI C'yCGr_G
Cuts at: 0 867 2017 2850 4389 4580 5501 5534 5661
Size: 867 1150 833 1539 191 921 33 127
Fragments arranged by size:
1539 1150 921 867 833 191 127 33
AvrII C'CTAG_G
Cuts at: 0 1041 5661
Size: 1041 4620
BamHI G'GATC_C
Cuts at: 0 1938 2012 2120 3512 4591 5661
Size: 1938 74 108 1392 1079 1070
Fragments arranged by size:
1938 1392 1079 1070 108 74
BanI G'GyrC_C
Cuts at: 0 1317 1408 2640 3544 3674 4622 5052 5137
Size: 1317 91 1232 904 130 948 430 85
Cuts at: 5137 5661
Size: 524
Fragments arranged by size:
1317 1232 948 904 524 430 130 91
85
BanII G_rGCy'C
Cuts at: 0 61 383 646 874 1434 1604 1849 1972
Size: 61 322 263 228 560 170 245 123
Cuts at: 1972 2666 2695 2741 2857 3422 4073 4147 4315
Size: 694 29 46 116 565 651 74 168
Cuts at: 4315 4528 5375 5644 5661
Size: 213 847 269 17
Fragments arranged by size:
847 694 651 565 560 322 269 263
245 228 213 170 168 123 116 74
61 46 29 17
BbeI G_GCGC'C
Cuts at: 0 1412 5661
Size: 1412 4249
BbsI GAAGACnn'nnnn_
Cuts at: 0 459 640 1454 2883 2983 4236 4990 5661
Size: 459 181 814 1429 100 1253 754 671
Fragments arranged by size:
1429 1253 814 754 671 459 181 100
BbvCI CC'TCA_GC
Cuts at: 0 2051 2602 3757 5661
Size: 2051 551 1155 1904
Fragments arranged by size:
2051 1904 1155 551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 270 304 1506 1540 4042 4076 5661
Size: 270 34 1202 34 2502 34 1585
Fragments arranged by size:
2502 1585 1202 270 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1030 1379 4122 5661
Size: 1030 349 2743 1539
Fragments arranged by size:
2743 1539 1030 349
BclI T'GATC_A
Cuts at: 0 5471 5661
Size: 5471 190
BfrBI ATG'CAT
Cuts at: 0 5596 5661
Size: 5596 65
BglI GCCn_nnn'nGGC
Cuts at: 0 73 4180 4505 5083 5191 5452 5661
Size: 73 4107 325 578 108 261 209
Fragments arranged by size:
4107 578 325 261 209 108 73
BlpI GC'TnA_GC
Cuts at: 0 2462 5661
Size: 2462 3199
Bme1580I G_kGCm'C
Cuts at: 0 61 874 1239 1434 2840 3547 4315 4625
Size: 61 813 365 195 1406 707 768 310
Cuts at: 4625 4777 5057 5142 5375 5661
Size: 152 280 85 233 286
Fragments arranged by size:
1406 813 768 707 365 310 286 280
233 195 152 85 61
BmgBI CAC'GTC
Cuts at: 0 920 1884 2682 4470 5661
Size: 920 964 798 1788 1191
Fragments arranged by size:
1788 1191 964 920 798
BmrI ACTGGGnnnn_n'
Cuts at: 0 2326 4833 5332 5661
Size: 2326 2507 499 329
Fragments arranged by size:
2507 2326 499 329
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1215 5410 5661
Size: 1215 4195 251
Fragments arranged by size:
4195 1215 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 409 424 1099 1678 4256 4542 4998
Size: 160 249 15 675 579 2578 286 456
Cuts at: 4998 5173 5289 5661
Size: 175 116 372
Fragments arranged by size:
2578 675 579 456 372 286 249 175
160 116 15
Bpu10I CC'TnA_GC
Cuts at: 0 2051 2602 2757 3499 3757 4972 5661
Size: 2051 551 155 742 258 1215 689
Fragments arranged by size:
2051 1215 742 689 551 258 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1737 1912 4856 5661
Size: 637 1100 175 2944 805
Fragments arranged by size:
2944 1100 805 637 175
BsaI GGTCTCn'nnnn_
Cuts at: 0 875 1075 3436 4810 5074 5661
Size: 875 200 2361 1374 264 587
Fragments arranged by size:
2361 1374 875 587 264 200
BsaAI yAC'GTr
Cuts at: 0 557 1023 1642 2922 5661
Size: 557 466 619 1280 2739
Fragments arranged by size:
2739 1280 619 557 466
BsaBI GATnn'nnATC
Cuts at: 0 3556 5661
Size: 3556 2105
BsaHI Gr'CG_yC
Cuts at: 0 749 1409 1820 3653 3685 5249 5661
Size: 749 660 411 1833 32 1564 412
Fragments arranged by size:
1833 1564 749 660 412 411 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 1972 2002 3580 3610 4882 4912
Size: 541 30 1401 30 1578 30 1272 30
Cuts at: 4912 5661
Size: 749
Fragments arranged by size:
1578 1401 1272 749 541 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1935 1962 4518 5661
Size: 1935 27 2556 1143
Fragments arranged by size:
2556 1935 1143 27
BseYI C'CCAG_C
Cuts at: 0 204 260 942 1372 2182 2282 2901 3311
Size: 204 56 682 430 810 100 619 410
Cuts at: 3311 3373 4618 5224 5455 5643 5661
Size: 62 1245 606 231 188 18
Fragments arranged by size:
1245 810 682 619 606 430 410 231
204 188 100 62 56 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 808 917 1576 2500 3445 3499 3962
Size: 265 543 109 659 924 945 54 463
Cuts at: 3962 4138 4318 5478 5661
Size: 176 180 1160 183
Fragments arranged by size:
1160 945 924 659 543 463 265 183
180 176 109 54
BsiEI CG_ry'CG
Cuts at: 0 181 895 1089 3735 4853 5186 5246 5661
Size: 181 714 194 2646 1118 333 60 415
Fragments arranged by size:
2646 1118 714 415 333 194 181 60
BsiHKAI G_wGCw'C
Cuts at: 0 229 383 646 1199 1811 2666 2741 2840
Size: 229 154 263 553 612 855 75 99
Cuts at: 2840 3731 4388 4475 4528 4777 5661
Size: 891 657 87 53 249 884
Fragments arranged by size:
891 884 855 657 612 553 263 249
229 154 99 87 75 53
BsmI GAATG_Cn'
Cuts at: 0 5194 5661
Size: 5194 467
BsmBI CGTCTCn'nnnn_
Cuts at: 0 369 4280 5256 5661
Size: 369 3911 976 405
Fragments arranged by size:
3911 976 405 369
Bsp1286I G_dGCh'C
Cuts at: 0 61 229 383 646 874 1199 1239 1434
Size: 61 168 154 263 228 325 40 195
Cuts at: 1434 1604 1811 1849 1972 2666 2695 2741 2840
Size: 170 207 38 123 694 29 46 99
Cuts at: 2840 2857 3422 3547 3731 4073 4147 4315 4388
Size: 17 565 125 184 342 74 168 73
Cuts at: 4388 4475 4528 4625 4777 5057 5142 5375 5644
Size: 87 53 97 152 280 85 233 269
Cuts at: 5644 5661
Size: 17
Fragments arranged by size:
694 565 342 325 280 269 263 233
228 207 195 184 170 168 168 154
152 125 123 99 97 87 85 74
73 61 53 46 40 38 29 17
17
BspHI T'CATG_A
Cuts at: 0 2867 5468 5661
Size: 2867 2601 193
Fragments arranged by size:
2867 2601 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1928 2142 2313 3279 3471 5028 5661
Size: 1928 214 171 966 192 1557 633
Fragments arranged by size:
1928 1557 966 633 214 192 171
BsrDI GCAATG_nn'
Cuts at: 0 1907 3351 3612 5327 5596 5661
Size: 1907 1444 261 1715 269 65
Fragments arranged by size:
1907 1715 1444 269 261 65
BsrFI r'CCGG_y
Cuts at: 0 894 1084 2390 3445 3568 4848 5661
Size: 894 190 1306 1055 123 1280 813
Fragments arranged by size:
1306 1280 1055 894 813 190 123
BsrGI T'GTAC_A
Cuts at: 0 2942 5661
Size: 2942 2719
BssSI C'ACGA_G
Cuts at: 0 2542 3421 3475 3679 5661
Size: 2542 879 54 204 1982
Fragments arranged by size:
2542 1982 879 204 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2523 2844 3436 5661
Size: 2523 321 592 2225
Fragments arranged by size:
2523 2225 592 321
BstEII G'GTnAC_C
Cuts at: 0 1001 1710 1959 2703 3291 5661
Size: 1001 709 249 744 588 2370
Fragments arranged by size:
2370 1001 744 709 588 249
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1707 2478 4461 5661
Size: 1707 771 1983 1200
Fragments arranged by size:
1983 1707 1200 771
BstYI r'GATC_y
Cuts at: 0 413 1938 2012 2120 3512 3830 4591 5505
Size: 413 1525 74 108 1392 318 761 914
Cuts at: 5505 5661
Size: 156
Fragments arranged by size:
1525 1392 914 761 413 318 156 108
74
Bsu36I CC'TnA_GG
Cuts at: 0 1669 4129 4627 5628 5661
Size: 1669 2460 498 1001 33
Fragments arranged by size:
2460 1669 1001 498 33
BtgI C'CryG_G
Cuts at: 0 1390 1434 2219 2831 3138 4454 4586 4739
Size: 1390 44 785 612 307 1316 132 153
Cuts at: 4739 4886 5261 5367 5661
Size: 147 375 106 294
Fragments arranged by size:
1390 1316 785 612 375 307 294 153
147 132 106 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1210 2142 5661
Size: 1210 932 3519
Fragments arranged by size:
3519 1210 932
BtsI GCAGTG_nn'
Cuts at: 0 229 245 929 934 1449 1524 1809 2567
Size: 229 16 684 5 515 75 285 758
Cuts at: 2567 2573 3265 3437 4360 4904 5661
Size: 6 692 172 923 544 757
Fragments arranged by size:
923 758 757 692 684 544 515 285
229 172 75 16 6 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1842 1877 5661
Size: 1842 35 3784
Fragments arranged by size:
3784 1842 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1744 5661
Size: 1744 3917
EaeI y'GGCC_r
Cuts at: 0 74 178 188 892 966 1086 1256 1878
Size: 74 104 10 704 74 120 170 622
Cuts at: 1878 2447 2502 3146 3153 3732 3809 4181 4481
Size: 569 55 644 7 579 77 372 300
Cuts at: 4481 4532 4850 4914 5183 5661
Size: 51 318 64 269 478
Fragments arranged by size:
704 644 622 579 569 478 372 318
300 269 170 120 104 77 74 74
64 55 51 10 7
EagI C'GGCC_G
Cuts at: 0 178 892 1086 3732 4850 5183 5661
Size: 178 714 194 2646 1118 333 478
Fragments arranged by size:
2646 1118 714 478 333 194 178
EarI CTCTTCn'nnn_
Cuts at: 0 2693 5077 5401 5446 5661
Size: 2693 2384 324 45 215
Fragments arranged by size:
2693 2384 324 215 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 54 514 5661
Size: 54 460 5147
Fragments arranged by size:
5147 460 54
EcoICRI GAG'CTC
Cuts at: 0 381 644 2664 2739 4526 5661
Size: 381 263 2020 75 1787 1135
Fragments arranged by size:
2020 1787 1135 381 263 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 409 424 429 430 838 1099 1437
Size: 160 249 15 5 1 408 261 338
Cuts at: 1437 1678 2388 3076 4256 4542 4998 5043 5173
Size: 241 710 688 1180 286 456 45 130
Cuts at: 5173 5289 5661
Size: 116 372
Fragments arranged by size:
1180 710 688 456 408 372 338 286
261 249 241 160 130 116 45 15
5 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2674 3478 4128 5627 5661
Size: 2674 804 650 1499 34
Fragments arranged by size:
2674 1499 804 650 34
EcoO109I rG'GnC_Cy
Cuts at: 0 58 315 420 871 1430 1653 1999 3387
Size: 58 257 105 451 559 223 346 1388
Cuts at: 3387 3471 4173 4311 4312 4631 5177 5278 5661
Size: 84 702 138 1 319 546 101 383
Fragments arranged by size:
1388 702 559 546 451 383 346 319
257 223 138 105 101 84 58 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3039 5661
Size: 3039 2622
HaeII r_GCGC'y
Cuts at: 0 1412 3371 5661
Size: 1412 1959 2290
Fragments arranged by size:
2290 1959 1412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 1930 1962 1997 2029 3185 3217
Size: 493 32 1405 32 35 32 1156 32
Cuts at: 3217 3772 3804 4738 4770 4852 4884 5155 5187
Size: 555 32 934 32 82 32 271 32
Cuts at: 5187 5661
Size: 474
Fragments arranged by size:
1405 1156 934 555 493 474 271 82
35 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 129 2958 5661
Size: 129 2829 2703
Fragments arranged by size:
2829 2703 129
HpaI GTT'AAC
Cuts at: 0 129 5661
Size: 129 5532
KasI G'GCGC_C
Cuts at: 0 1408 5661
Size: 1408 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2906 3347 3717 5661
Size: 2906 441 370 1944
Fragments arranged by size:
2906 1944 441 370
MscI TGG'CCA
Cuts at: 0 190 968 2449 2504 3148 3811 4483 5661
Size: 190 778 1481 55 644 663 672 1178
Fragments arranged by size:
1481 1178 778 672 663 644 190 55
MslI CAynn'nnrTG
Cuts at: 0 619 922 1061 1279 1389 1574 1892 2671
Size: 619 303 139 218 110 185 318 779
Cuts at: 2671 2866 2950 3532 3550 3724 4001 4054 4459
Size: 195 84 582 18 174 277 53 405
Cuts at: 4459 5338 5661
Size: 879 323
Fragments arranged by size:
879 779 619 582 405 323 318 303
277 218 195 185 174 139 110 84
53 18
MspA1I CmG'CkG
Cuts at: 0 70 164 204 942 1457 1817 2050 2286
Size: 70 94 40 738 515 360 233 236
Cuts at: 2286 2304 2478 3140 3798 3861 4829 4962 5263
Size: 18 174 662 658 63 968 133 301
Cuts at: 5263 5464 5661
Size: 201 197
Fragments arranged by size:
968 738 662 658 515 360 301 236
233 201 197 174 133 94 70 63
40 18
NaeI GCC'GGC
Cuts at: 0 4850 5661
Size: 4850 811
NarI GG'CG_CC
Cuts at: 0 1409 5661
Size: 1409 4252
NcoI C'CATG_G
Cuts at: 0 2831 4454 5661
Size: 2831 1623 1207
Fragments arranged by size:
2831 1623 1207
NgoMIV G'CCGG_C
Cuts at: 0 4848 5661
Size: 4848 813
NsiI A_TGCA'T
Cuts at: 0 5598 5661
Size: 5598 63
NspI r_CATG'y
Cuts at: 0 987 2295 4000 5661
Size: 987 1308 1705 1661
Fragments arranged by size:
1705 1661 1308 987
PasI CC'CwG_GG
Cuts at: 0 1783 1842 1965 5661
Size: 1783 59 123 3696
Fragments arranged by size:
3696 1783 123 59
PciI A'CATG_T
Cuts at: 0 2291 5661
Size: 2291 3370
PflMI CCAn_nnn'nTGG
Cuts at: 0 423 1611 1656 2009 5661
Size: 423 1188 45 353 3652
Fragments arranged by size:
3652 1188 423 353 45
PfoI T'CCnGG_A
Cuts at: 0 665 2098 3656 3965 4706 5408 5661
Size: 665 1433 1558 309 741 702 253
Fragments arranged by size:
1558 1433 741 702 665 309 253
PmlI CAC'GTG
Cuts at: 0 1642 5661
Size: 1642 4019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3580 3612 5661
Size: 3580 32 2049
Fragments arranged by size:
3580 2049 32
PpuMI rG'GwC_Cy
Cuts at: 0 315 1999 3471 4631 5177 5661
Size: 315 1684 1472 1160 546 484
Fragments arranged by size:
1684 1472 1160 546 484 315
PshAI GACnn'nnGTC
Cuts at: 0 3699 5464 5661
Size: 3699 1765 197
Fragments arranged by size:
3699 1765 197
PspOMI G'GGCC_C
Cuts at: 0 57 870 1430 4311 5371 5661
Size: 57 813 560 2881 1060 290
Fragments arranged by size:
2881 1060 813 560 290 57
PspXI vC'TCGA_Gb
Cuts at: 0 2017 5534 5661
Size: 2017 3517 127
Fragments arranged by size:
3517 2017 127
PstI C_TGCA'G
Cuts at: 0 699 941 1816 2312 3860 3926 5523 5661
Size: 699 242 875 496 1548 66 1597 138
Fragments arranged by size:
1597 1548 875 699 496 242 138 66
PvuII CAG'CTG
Cuts at: 0 942 1457 2286 2478 3798 3861 4829 5661
Size: 942 515 829 192 1320 63 968 832
Fragments arranged by size:
1320 968 942 832 829 515 192 63
RsrII CG'GwC_CG
Cuts at: 0 3749 5661
Size: 3749 1912
SacI G_AGCT'C
Cuts at: 0 383 646 2666 2741 4528 5661
Size: 383 263 2020 75 1787 1133
Fragments arranged by size:
2020 1787 1133 383 263 75
SacII CC_GC'GG
Cuts at: 0 3141 5264 5661
Size: 3141 2123 397
Fragments arranged by size:
3141 2123 397
SanDI GG'GwC_CC
Cuts at: 0 5177 5661
Size: 5177 484
SapI GCTCTTCn'nnn_
Cuts at: 0 5446 5661
Size: 5446 215
ScaI AGT'ACT
Cuts at: 0 4921 5661
Size: 4921 740
SexAI A'CCwGG_T
Cuts at: 0 1475 5420 5661
Size: 1475 3945 241
Fragments arranged by size:
3945 1475 241
SfcI C'TryA_G
Cuts at: 0 695 937 1312 1812 1869 2308 3856 3922
Size: 695 242 375 500 57 439 1548 66
Cuts at: 3922 4423 4715 5519 5661
Size: 501 292 804 142
Fragments arranged by size:
1548 804 695 501 500 439 375 292
242 142 66 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4180 5661
Size: 4180 1481
SfoI GGC'GCC
Cuts at: 0 1410 5661
Size: 1410 4251
SmaI CCC'GGG
Cuts at: 0 869 2852 4391 4582 5503 5661
Size: 869 1983 1539 191 921 158
Fragments arranged by size:
1983 1539 921 869 191 158
SmlI C'TyrA_G
Cuts at: 0 652 1716 1927 2017 4871 5534 5661
Size: 652 1064 211 90 2854 663 127
Fragments arranged by size:
2854 1064 663 652 211 127 90
SnaBI TAC'GTA
Cuts at: 0 557 5661
Size: 557 5104
SphI G_CATG'C
Cuts at: 0 987 5661
Size: 987 4674
SrfI GCCC'GGGC
Cuts at: 0 4582 5661
Size: 4582 1079
SspI AAT'ATT
Cuts at: 0 3893 5661
Size: 3893 1768
StuI AGG'CCT
Cuts at: 0 145 2140 3620 4508 4804 5661
Size: 145 1995 1480 888 296 857
Fragments arranged by size:
1995 1480 888 857 296 145
StyI C'CwwG_G
Cuts at: 0 963 1041 1955 2008 2141 2831 2906 4454
Size: 963 78 914 53 133 690 75 1548
Cuts at: 4454 5274 5661
Size: 820 387
Fragments arranged by size:
1548 963 914 820 690 387 133 78
75 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1062 2134 3562 4174 4251 4798 4826 5207
Size: 1062 1072 1428 612 77 547 28 381
Cuts at: 5207 5595 5661
Size: 388 66
Fragments arranged by size:
1428 1072 1062 612 547 388 381 77
66 28
TatI w'GTAC_w
Cuts at: 0 1418 2942 3707 4919 5661
Size: 1418 1524 765 1212 742
Fragments arranged by size:
1524 1418 1212 765 742
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1353 1385 4187 4219 5661
Size: 1353 32 2802 32 1442
Fragments arranged by size:
2802 1442 1353 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 36 5661
Size: 36 5625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1441 2283 2442 4231 4566 5037 5661
Size: 1441 842 159 1789 335 471 624
Fragments arranged by size:
1789 1441 842 624 471 335 159
XhoI C'TCGA_G
Cuts at: 0 2017 5534 5661
Size: 2017 3517 127
Fragments arranged by size:
3517 2017 127
XmaI C'CCGG_G
Cuts at: 0 867 2850 4389 4580 5501 5661
Size: 867 1983 1539 191 921 160
Fragments arranged by size:
1983 1539 921 867 191 160
XmnI GAAnn'nnTTC
Cuts at: 0 442 3022 3230 3592 3983 5661
Size: 442 2580 208 362 391 1678
Fragments arranged by size:
2580 1678 442 391 362 208
ZraI GAC'GTC
Cuts at: 0 750 3654 5661
Size: 750 2904 2007
Fragments arranged by size:
2904 2007 750
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HpaI KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII RsrII SacI SacII SanDI
SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SnaBI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AscI AsiSI BaeI
BglII BmtI BsaWI BsiWI BspEI BsrBI BssHII BstBI
BstZ17I ClaI DraI DraIII EcoRI EcoRV FseI FspI
FspAI HindIII KpnI MfeI MluI NdeI NheI NotI
NruI PacI PmeI PsiI PsrI PvuI SalI SbfI
SgfI SgrAI SpeI SwaI XbaI