Restriction map for
pF1KA0900
(Linear) (Six-base) MAPSORT of: KIAA0900.seq Check: 2070 from: 1 to: 1506
KIAA0900.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 164 463 1506
Size: 77 87 299 1043
Fragments arranged by size:
1043 299 87 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1310 1506
Size: 1310 196
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1483 1506
Size: 1483 23
AlwNI CAG_nnn'CTG
Cuts at: 0 1273 1506
Size: 1273 233
ApaI G_GGCC'C
Cuts at: 0 1198 1506
Size: 1198 308
ApoI r'AATT_y
Cuts at: 0 198 1352 1506
Size: 198 1154 154
Fragments arranged by size:
1154 198 154
AvaI C'yCGr_G
Cuts at: 0 1191 1228 1506
Size: 1191 37 278
Fragments arranged by size:
1191 278 37
BamHI G'GATC_C
Cuts at: 0 694 1506
Size: 694 812
BanI G'GyrC_C
Cuts at: 0 559 602 1506
Size: 559 43 904
Fragments arranged by size:
904 559 43
BanII G_rGCy'C
Cuts at: 0 29 51 1169 1198 1506
Size: 29 22 1118 29 308
Fragments arranged by size:
1118 308 29 29 22
BbeI G_GCGC'C
Cuts at: 0 606 1506
Size: 606 900
BbsI GAAGACnn'nnnn_
Cuts at: 0 152 515 677 1226 1506
Size: 152 363 162 549 280
Fragments arranged by size:
549 363 280 162 152
BbvCI CC'TCA_GC
Cuts at: 0 902 1059 1274 1506
Size: 902 157 215 232
Fragments arranged by size:
902 232 215 157
BfrBI ATG'CAT
Cuts at: 0 724 1257 1506
Size: 724 533 249
Fragments arranged by size:
724 533 249
BlpI GC'TnA_GC
Cuts at: 0 121 1506
Size: 121 1385
Bme1580I G_kGCm'C
Cuts at: 0 1198 1506
Size: 1198 308
BmgBI CAC'GTC
Cuts at: 0 813 1419 1506
Size: 813 606 87
Fragments arranged by size:
813 606 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 1261 1506
Size: 1261 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 1072 1506
Size: 953 119 434
Fragments arranged by size:
953 434 119
Bpu10I CC'TnA_GC
Cuts at: 0 902 1059 1274 1506
Size: 902 157 215 232
Fragments arranged by size:
902 232 215 157
BsaI GGTCTCn'nnnn_
Cuts at: 0 273 1506
Size: 273 1233
BsaHI Gr'CG_yC
Cuts at: 0 531 603 1506
Size: 531 72 903
Fragments arranged by size:
903 531 72
BsaWI w'CCGG_w
Cuts at: 0 1021 1506
Size: 1021 485
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 838 868 1141 1171 1506
Size: 838 30 273 30 335
Fragments arranged by size:
838 335 273 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1022 1034 1133 1136 1321 1506
Size: 1022 12 99 3 185 185
Fragments arranged by size:
1022 185 185 99 12 3
BseYI C'CCAG_C
Cuts at: 0 242 779 990 1506
Size: 242 537 211 516
Fragments arranged by size:
537 516 242 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 941 1280 1390 1506
Size: 757 184 339 110 116
Fragments arranged by size:
757 339 184 116 110
BsiHKAI G_wGCw'C
Cuts at: 0 51 1506
Size: 51 1455
BsmI GAATG_Cn'
Cuts at: 0 347 1506
Size: 347 1159
Bsp1286I G_dGCh'C
Cuts at: 0 29 51 1169 1198 1506
Size: 29 22 1118 29 308
Fragments arranged by size:
1118 308 29 29 22
BspEI T'CCGG_A
Cuts at: 0 1021 1506
Size: 1021 485
BsrDI GCAATG_nn'
Cuts at: 0 1442 1506
Size: 1442 64
BsrFI r'CCGG_y
Cuts at: 0 857 1506
Size: 857 649
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1069 1506
Size: 1069 437
BstBI TT'CG_AA
Cuts at: 0 1344 1506
Size: 1344 162
BstYI r'GATC_y
Cuts at: 0 694 1506
Size: 694 812
Bsu36I CC'TnA_GG
Cuts at: 0 929 1506
Size: 929 577
BtgI C'CryG_G
Cuts at: 0 675 1448 1506
Size: 675 773 58
Fragments arranged by size:
773 675 58
EaeI y'GGCC_r
Cuts at: 0 1421 1506
Size: 1421 85
EarI CTCTTCn'nnn_
Cuts at: 0 7 209 257 1506
Size: 7 202 48 1249
Fragments arranged by size:
1249 202 48 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1025 1506
Size: 1025 481
EcoICRI GAG'CTC
Cuts at: 0 49 1506
Size: 49 1457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 164 463 953 1072 1506
Size: 77 87 299 490 119 434
Fragments arranged by size:
490 434 299 119 87 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 876 1506
Size: 876 630
EcoO109I rG'GnC_Cy
Cuts at: 0 933 944 1216 1506
Size: 933 11 272 290
Fragments arranged by size:
933 290 272 11
EcoRI G'AATT_C
Cuts at: 0 1352 1506
Size: 1352 154
EcoRV GAT'ATC
Cuts at: 0 282 1506
Size: 282 1224
HaeII r_GCGC'y
Cuts at: 0 606 1506
Size: 606 900
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 700 732 1459 1491 1506
Size: 700 32 727 32 15
Fragments arranged by size:
727 700 32 32 15
HindIII A'AGCT_T
Cuts at: 0 296 1506
Size: 296 1210
KasI G'GCGC_C
Cuts at: 0 602 1506
Size: 602 904
MscI TGG'CCA
Cuts at: 0 1423 1506
Size: 1423 83
MslI CAynn'nnrTG
Cuts at: 0 1447 1506
Size: 1447 59
MspA1I CmG'CkG
Cuts at: 0 120 246 805 1380 1506
Size: 120 126 559 575 126
Fragments arranged by size:
575 559 126 126 120
NarI GG'CG_CC
Cuts at: 0 603 1506
Size: 603 903
NcoI C'CATG_G
Cuts at: 0 675 1506
Size: 675 831
NdeI CA'TA_TG
Cuts at: 0 627 1506
Size: 627 879
NsiI A_TGCA'T
Cuts at: 0 726 1259 1506
Size: 726 533 247
Fragments arranged by size:
726 533 247
NspI r_CATG'y
Cuts at: 0 142 724 1506
Size: 142 582 782
Fragments arranged by size:
782 582 142
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 1258 1506
Size: 330 928 248
Fragments arranged by size:
928 330 248
PpuMI rG'GwC_Cy
Cuts at: 0 944 1506
Size: 944 562
PspOMI G'GGCC_C
Cuts at: 0 1194 1506
Size: 1194 312
PvuII CAG'CTG
Cuts at: 0 120 246 805 1506
Size: 120 126 559 701
Fragments arranged by size:
701 559 126 120
SacI G_AGCT'C
Cuts at: 0 51 1506
Size: 51 1455
SanDI GG'GwC_CC
Cuts at: 0 944 1506
Size: 944 562
ScaI AGT'ACT
Cuts at: 0 96 1506
Size: 96 1410
SfcI C'TryA_G
Cuts at: 0 274 496 1506
Size: 274 222 1010
Fragments arranged by size:
1010 274 222
SfoI GGC'GCC
Cuts at: 0 604 1506
Size: 604 902
SmaI CCC'GGG
Cuts at: 0 1193 1230 1506
Size: 1193 37 276
Fragments arranged by size:
1193 276 37
StuI AGG'CCT
Cuts at: 0 952 1506
Size: 952 554
StyI C'CwwG_G
Cuts at: 0 675 1506
Size: 675 831
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 256 1166 1506
Size: 256 910 340
Fragments arranged by size:
910 340 256
TatI w'GTAC_w
Cuts at: 0 94 1454 1506
Size: 94 1360 52
Fragments arranged by size:
1360 94 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1141 1173 1506
Size: 1141 32 333
Fragments arranged by size:
1141 333 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1167 1506
Size: 1167 339
XmaI C'CCGG_G
Cuts at: 0 1191 1228 1506
Size: 1191 37 278
Fragments arranged by size:
1191 278 37
Enzymes that do cut:
AcuI AlfI AloI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BfrBI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI
BsrFI BstAPI BstBI BstYI Bsu36I BtgI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HindIII KasI MscI MslI MspA1I NarI NcoI
NdeI NsiI NspI PflMI PpuMI PspOMI PvuII SacI
SanDI ScaI SfcI SfoI SmaI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaLI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BglI BglII BmtI BplI
BpuEI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI FalI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
MmeI NaeI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SapI SbfI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI