Restriction map for
pF1KSDA0888
(Linear) (Six-base) MAPSORT of: KIAA0888.seq Check: 5309 from: 1 to: 2010
KIAA0888.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 306 2010
Size: 306 1704
AccI GT'mk_AC
Cuts at: 0 664 1111 1473 1940 2010
Size: 664 447 362 467 70
Fragments arranged by size:
664 467 447 362 70
Acc65I G'GTAC_C
Cuts at: 0 743 2010
Size: 743 1267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 236 899 1865 2010
Size: 80 156 663 966 145
Fragments arranged by size:
966 663 156 145 80
AflII C'TTAA_G
Cuts at: 0 310 1306 2010
Size: 310 996 704
Fragments arranged by size:
996 704 310
AflIII A'CryG_T
Cuts at: 0 68 2010
Size: 68 1942
AgeI A'CCGG_T
Cuts at: 0 1662 2010
Size: 1662 348
AhdI GACnn_n'nnGTC
Cuts at: 0 888 2010
Size: 888 1122
ApoI r'AATT_y
Cuts at: 0 51 194 791 1036 1141 1177 1648 1674
Size: 51 143 597 245 105 36 471 26
Cuts at: 1674 1950 2010
Size: 276 60
Fragments arranged by size:
597 471 276 245 143 105 60 51
36 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 727 760 2010
Size: 727 33 1250
Fragments arranged by size:
1250 727 33
BanI G'GyrC_C
Cuts at: 0 743 2010
Size: 743 1267
BanII G_rGCy'C
Cuts at: 0 1135 1736 2010
Size: 1135 601 274
Fragments arranged by size:
1135 601 274
BbsI GAAGACnn'nnnn_
Cuts at: 0 644 887 1340 1400 1712 1796 2010
Size: 644 243 453 60 312 84 214
Fragments arranged by size:
644 453 312 243 214 84 60
BciVI GTATCCnnnnn_n'
Cuts at: 0 653 704 2010
Size: 653 51 1306
Fragments arranged by size:
1306 653 51
BclI T'GATC_A
Cuts at: 0 252 2010
Size: 252 1758
BlpI GC'TnA_GC
Cuts at: 0 2000 2010
Size: 2000 10
Bme1580I G_kGCm'C
Cuts at: 0 1526 2010
Size: 1526 484
BmgBI CAC'GTC
Cuts at: 0 1794 2010
Size: 1794 216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1353 2010
Size: 1353 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 854 2010
Size: 616 238 1156
Fragments arranged by size:
1156 616 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 2010
Size: 1034 976
BsaI GGTCTCn'nnnn_
Cuts at: 0 695 1671 2010
Size: 695 976 339
Fragments arranged by size:
976 695 339
BsaAI yAC'GTr
Cuts at: 0 1471 2010
Size: 1471 539
BsaWI w'CCGG_w
Cuts at: 0 1662 2010
Size: 1662 348
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1732 1849 2010
Size: 1732 117 161
Fragments arranged by size:
1732 161 117
BseYI C'CCAG_C
Cuts at: 0 1226 2010
Size: 1226 784
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 2010
Size: 1100 910
BsiHKAI G_wGCw'C
Cuts at: 0 325 1135 2010
Size: 325 810 875
Fragments arranged by size:
875 810 325
BsmI GAATG_Cn'
Cuts at: 0 4 1815 2010
Size: 4 1811 195
Fragments arranged by size:
1811 195 4
Bsp1286I G_dGCh'C
Cuts at: 0 325 1135 1526 1736 2010
Size: 325 810 391 210 274
Fragments arranged by size:
810 391 325 274 210
BspHI T'CATG_A
Cuts at: 0 954 2010
Size: 954 1056
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 306 1992 2010
Size: 306 1686 18
Fragments arranged by size:
1686 306 18
BsrFI r'CCGG_y
Cuts at: 0 1662 2010
Size: 1662 348
BstYI r'GATC_y
Cuts at: 0 398 2010
Size: 398 1612
BstZ17I GTA'TAC
Cuts at: 0 665 1474 2010
Size: 665 809 536
Fragments arranged by size:
809 665 536
Bsu36I CC'TnA_GG
Cuts at: 0 1442 2010
Size: 1442 568
BtsI GCAGTG_nn'
Cuts at: 0 1200 2010
Size: 1200 810
EaeI y'GGCC_r
Cuts at: 0 1003 1210 2010
Size: 1003 207 800
Fragments arranged by size:
1003 800 207
EarI CTCTTCn'nnn_
Cuts at: 0 122 1322 1526 1760 2010
Size: 122 1200 204 234 250
Fragments arranged by size:
1200 250 234 204 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1962 2010
Size: 1962 48
EcoICRI GAG'CTC
Cuts at: 0 1133 2010
Size: 1133 877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 236 616 854 899 1865 2010
Size: 80 156 380 238 45 966 145
Fragments arranged by size:
966 380 238 156 145 80 45
EcoO109I rG'GnC_Cy
Cuts at: 0 90 1981 2010
Size: 90 1891 29
Fragments arranged by size:
1891 90 29
EcoRI G'AATT_C
Cuts at: 0 1141 2010
Size: 1141 869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 646 699 1585 2010
Size: 646 53 886 425
Fragments arranged by size:
886 646 425 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 874 906 949 981 1525 1557
Size: 55 32 787 32 43 32 544 32
Cuts at: 1557 1663 1695 1711 1743 1825 1857 1891 1923
Size: 106 32 16 32 82 32 34 32
Cuts at: 1923 2010
Size: 87
Fragments arranged by size:
787 544 106 87 82 55 43 34
32 32 32 32 32 32 32 32
16
KpnI G_GTAC'C
Cuts at: 0 747 2010
Size: 747 1263
MfeI C'AATT_G
Cuts at: 0 526 2010
Size: 526 1484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 714 2010
Size: 714 1296
MslI CAynn'nnrTG
Cuts at: 0 408 2010
Size: 408 1602
MspA1I CmG'CkG
Cuts at: 0 232 2010
Size: 232 1778
NdeI CA'TA_TG
Cuts at: 0 496 2010
Size: 496 1514
NspI r_CATG'y
Cuts at: 0 72 594 2010
Size: 72 522 1416
Fragments arranged by size:
1416 522 72
PciI A'CATG_T
Cuts at: 0 68 2010
Size: 68 1942
PflMI CCAn_nnn'nTGG
Cuts at: 0 1012 2010
Size: 1012 998
PfoI T'CCnGG_A
Cuts at: 0 696 1145 2010
Size: 696 449 865
Fragments arranged by size:
865 696 449
PpuMI rG'GwC_Cy
Cuts at: 0 90 1981 2010
Size: 90 1891 29
Fragments arranged by size:
1891 90 29
PshAI GACnn'nnGTC
Cuts at: 0 828 2010
Size: 828 1182
PsiI TTA'TAA
Cuts at: 0 1321 2010
Size: 1321 689
PvuII CAG'CTG
Cuts at: 0 232 2010
Size: 232 1778
SacI G_AGCT'C
Cuts at: 0 1135 2010
Size: 1135 875
SanDI GG'GwC_CC
Cuts at: 0 90 2010
Size: 90 1920
SapI GCTCTTCn'nnn_
Cuts at: 0 1760 2010
Size: 1760 250
ScaI AGT'ACT
Cuts at: 0 423 2010
Size: 423 1587
SfcI C'TryA_G
Cuts at: 0 485 2010
Size: 485 1525
SmlI C'TyrA_G
Cuts at: 0 310 1049 1306 2010
Size: 310 739 257 704
Fragments arranged by size:
739 704 310 257
SpeI A'CTAG_T
Cuts at: 0 1342 2010
Size: 1342 668
SspI AAT'ATT
Cuts at: 0 126 2010
Size: 126 1884
TatI w'GTAC_w
Cuts at: 0 421 2010
Size: 421 1589
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AgeI AhdI
ApoI BaeI BanI BanII BbsI BciVI BclI BlpI
Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaAI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrFI BstYI BstZ17I Bsu36I BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I KpnI MfeI
MmeI MslI MspA1I NdeI NspI PciI PflMI PfoI
PpuMI PshAI PsiI PvuII SacI SanDI SapI ScaI
SfcI SmlI SpeI SspI TatI
Enzymes that do not cut:
AatII AclI AfeI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI
BbvCI BcgI BfrBI BglI BglII BmrI BmtI Bpu10I
BsaBI BsaHI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoNI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI StuI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI