Restriction map for
pF1KSDA0885
(Linear) (Six-base) MAPSORT of: KIAA0885.seq Check: 5592 from: 1 to: 2394
KIAA0885.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
Acc65I G'GTAC_C
Cuts at: 0 44 2394
Size: 44 2350
AclI AA'CG_TT
Cuts at: 0 446 2394
Size: 446 1948
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 308 449 1110 1597 2394
Size: 44 264 141 661 487 797
Fragments arranged by size:
797 661 487 264 141 44
AleI CACnn'nnGTG
Cuts at: 0 638 1127 2394
Size: 638 489 1267
Fragments arranged by size:
1267 638 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 544 2394
Size: 544 1850
AlwNI CAG_nnn'CTG
Cuts at: 0 2195 2394
Size: 2195 199
ApaI G_GGCC'C
Cuts at: 0 2224 2394
Size: 2224 170
ApoI r'AATT_y
Cuts at: 0 208 643 688 1133 1468 2394
Size: 208 435 45 445 335 926
Fragments arranged by size:
926 445 435 335 208 45
AvaI C'yCGr_G
Cuts at: 0 357 1040 2394
Size: 357 683 1354
Fragments arranged by size:
1354 683 357
BanI G'GyrC_C
Cuts at: 0 44 2394
Size: 44 2350
BanII G_rGCy'C
Cuts at: 0 2224 2394
Size: 2224 170
BbvCI CC'TCA_GC
Cuts at: 0 1361 2394
Size: 1361 1033
Bme1580I G_kGCm'C
Cuts at: 0 1249 1291 2224 2394
Size: 1249 42 933 170
Fragments arranged by size:
1249 933 170 42
BmgBI CAC'GTC
Cuts at: 0 973 2394
Size: 973 1421
BmrI ACTGGGnnnn_n'
Cuts at: 0 89 245 818 1681 2394
Size: 89 156 573 863 713
Fragments arranged by size:
863 713 573 156 89
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 362 2394
Size: 362 2032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 482 2394
Size: 332 150 1912
Fragments arranged by size:
1912 332 150
Bpu10I CC'TnA_GC
Cuts at: 0 1361 2394
Size: 1361 1033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 2394
Size: 731 1663
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 188 218 1448 1478 2132 2162 2394
Size: 188 30 1230 30 654 30 232
Fragments arranged by size:
1230 654 232 188 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1436 1830 1882 2231 2291 2394
Size: 1436 394 52 349 60 103
Fragments arranged by size:
1436 394 349 103 60 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1745 2208 2378 2394
Size: 1745 463 170 16
Fragments arranged by size:
1745 463 170 16
BsiHKAI G_wGCw'C
Cuts at: 0 519 2301 2394
Size: 519 1782 93
Fragments arranged by size:
1782 519 93
Bsp1286I G_dGCh'C
Cuts at: 0 519 1249 1291 2224 2301 2394
Size: 519 730 42 933 77 93
Fragments arranged by size:
933 730 519 93 77 42
BspHI T'CATG_A
Cuts at: 0 1611 2394
Size: 1611 783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 1494 2394
Size: 185 1309 900
Fragments arranged by size:
1309 900 185
BsrDI GCAATG_nn'
Cuts at: 0 245 844 2394
Size: 245 599 1550
Fragments arranged by size:
1550 599 245
BstEII G'GTnAC_C
Cuts at: 0 921 937 2394
Size: 921 16 1457
Fragments arranged by size:
1457 921 16
BstXI CCAn_nnnn'nTGG
Cuts at: 0 31 1428 2394
Size: 31 1397 966
Fragments arranged by size:
1397 966 31
BstYI r'GATC_y
Cuts at: 0 1435 2369 2394
Size: 1435 934 25
Fragments arranged by size:
1435 934 25
Bsu36I CC'TnA_GG
Cuts at: 0 1813 2394
Size: 1813 581
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 692 2394
Size: 692 1702
BtsI GCAGTG_nn'
Cuts at: 0 67 2394
Size: 67 2327
DraIII CAC_nnn'GTG
Cuts at: 0 73 1752 2394
Size: 73 1679 642
Fragments arranged by size:
1679 642 73
DrdI GACnn_nn'nnGTC
Cuts at: 0 1529 2394
Size: 1529 865
EaeI y'GGCC_r
Cuts at: 0 1916 2394
Size: 1916 478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 308 332 449 482 1110 1597 2394
Size: 44 264 24 117 33 628 487 797
Fragments arranged by size:
797 628 487 264 117 44 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1451 2394
Size: 1451 943
EcoO109I rG'GnC_Cy
Cuts at: 0 2220 2221 2394
Size: 2220 1 173
Fragments arranged by size:
2220 173 1
EcoRI G'AATT_C
Cuts at: 0 688 2394
Size: 688 1706
EcoRV GAT'ATC
Cuts at: 0 771 2394
Size: 771 1623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 160 672 1572 1641 2109 2394
Size: 160 512 900 69 468 285
Fragments arranged by size:
900 512 468 285 160 69
FspI TGC'GCA
Cuts at: 0 2020 2394
Size: 2020 374
HaeII r_GCGC'y
Cuts at: 0 2195 2394
Size: 2195 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 328 360 898 930 1183 1215 1809
Size: 26 302 32 538 32 253 32 594
Cuts at: 1809 1841 2154 2186 2394
Size: 32 313 32 208
Fragments arranged by size:
594 538 313 302 253 208 32 32
32 32 32 26
HincII GTy'rAC
Cuts at: 0 101 867 2394
Size: 101 766 1527
Fragments arranged by size:
1527 766 101
HpaI GTT'AAC
Cuts at: 0 101 2394
Size: 101 2293
KpnI G_GTAC'C
Cuts at: 0 48 2394
Size: 48 2346
MfeI C'AATT_G
Cuts at: 0 1963 2394
Size: 1963 431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1852 2394
Size: 394 1458 542
Fragments arranged by size:
1458 542 394
MscI TGG'CCA
Cuts at: 0 1918 2394
Size: 1918 476
MslI CAynn'nnrTG
Cuts at: 0 29 503 638 1127 1637 2394
Size: 29 474 135 489 510 757
Fragments arranged by size:
757 510 489 474 135 29
MspA1I CmG'CkG
Cuts at: 0 352 453 554 2394
Size: 352 101 101 1840
Fragments arranged by size:
1840 352 101 101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2137 2169 2394
Size: 2137 32 225
Fragments arranged by size:
2137 225 32
PspOMI G'GGCC_C
Cuts at: 0 2220 2394
Size: 2220 174
PstI C_TGCA'G
Cuts at: 0 1940 2394
Size: 1940 454
PvuII CAG'CTG
Cuts at: 0 352 453 2394
Size: 352 101 1941
Fragments arranged by size:
1941 352 101
RsrII CG'GwC_CG
Cuts at: 0 225 2394
Size: 225 2169
SfcI C'TryA_G
Cuts at: 0 636 1936 2131 2394
Size: 636 1300 195 263
Fragments arranged by size:
1300 636 263 195
SmaI CCC'GGG
Cuts at: 0 359 2394
Size: 359 2035
SmlI C'TyrA_G
Cuts at: 0 746 2394
Size: 746 1648
SspI AAT'ATT
Cuts at: 0 957 2394
Size: 957 1437
StuI AGG'CCT
Cuts at: 0 1494 2394
Size: 1494 900
StyI C'CwwG_G
Cuts at: 0 917 1241 1421 1845 2225 2326 2394
Size: 917 324 180 424 380 101 68
Fragments arranged by size:
917 424 380 324 180 101 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 242 983 1039 2394
Size: 242 741 56 1355
Fragments arranged by size:
1355 741 242 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 296 328 2132 2164 2394
Size: 296 32 1804 32 230
Fragments arranged by size:
1804 296 230 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1681 2394
Size: 1681 713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1554 2394
Size: 1554 840
XmaI C'CCGG_G
Cuts at: 0 357 2394
Size: 357 2037
Enzymes that do cut:
Acc65I AclI AcuI AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbvCI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BstEII BstXI BstYI Bsu36I BtgZI
BtsI DraIII DrdI EaeI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KpnI
MfeI MmeI MscI MslI MspA1I PpiI PspOMI PstI
PvuII RsrII SfcI SmaI SmlI SspI StuI StyI
TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I BtgI ClaI CspCI
DraI EagI EarI EciI EcoICRI FseI FspAI HindIII
KasI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI
PvuI SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI TatI XbaI XhoI XmnI ZraI