Restriction map for
pF1KSDA0883
(Linear) (Six-base) MAPSORT of: KIAA0883.seq Check: 5881 from: 1 to: 1092
KIAA0883.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 784 964 1092
Size: 100 684 180 128
Fragments arranged by size:
684 180 128 100
AhdI GACnn_n'nnGTC
Cuts at: 0 675 1092
Size: 675 417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 197 1092
Size: 197 895
AlwNI CAG_nnn'CTG
Cuts at: 0 468 921 1092
Size: 468 453 171
Fragments arranged by size:
468 453 171
ApaI G_GGCC'C
Cuts at: 0 637 958 1092
Size: 637 321 134
Fragments arranged by size:
637 321 134
AvaI C'yCGr_G
Cuts at: 0 351 1092
Size: 351 741
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 206 239 1092
Size: 206 33 853
Fragments arranged by size:
853 206 33
BanI G'GyrC_C
Cuts at: 0 926 1092
Size: 926 166
BanII G_rGCy'C
Cuts at: 0 369 637 958 1092
Size: 369 268 321 134
Fragments arranged by size:
369 321 268 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 772 1092
Size: 772 320
BbvCI CC'TCA_GC
Cuts at: 0 461 1092
Size: 461 631
BciVI GTATCCnnnnn_n'
Cuts at: 0 68 1092
Size: 68 1024
BglI GCCn_nnn'nGGC
Cuts at: 0 632 1092
Size: 632 460
Bme1580I G_kGCm'C
Cuts at: 0 404 637 958 1092
Size: 404 233 321 134
Fragments arranged by size:
404 321 233 134
BmrI ACTGGGnnnn_n'
Cuts at: 0 230 1092
Size: 230 862
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 810 1092
Size: 810 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 904 1092
Size: 374 530 188
Fragments arranged by size:
530 374 188
Bpu10I CC'TnA_GC
Cuts at: 0 461 934 1092
Size: 461 473 158
Fragments arranged by size:
473 461 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1092
Size: 994 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 354 798 1092
Size: 354 444 294
Fragments arranged by size:
444 354 294
BsaWI w'CCGG_w
Cuts at: 0 167 485 827 1092
Size: 167 318 342 265
Fragments arranged by size:
342 318 265 167
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 776 806 1092
Size: 370 30 376 30 286
Fragments arranged by size:
376 370 286 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 1018 1092
Size: 106 912 74
Fragments arranged by size:
912 106 74
BseYI C'CCAG_C
Cuts at: 0 519 898 964 1092
Size: 519 379 66 128
Fragments arranged by size:
519 379 128 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 682 1092
Size: 48 634 410
Fragments arranged by size:
634 410 48
BsmI GAATG_Cn'
Cuts at: 0 186 1092
Size: 186 906
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1079 1092
Size: 1079 13
Bsp1286I G_dGCh'C
Cuts at: 0 369 404 637 958 1092
Size: 369 35 233 321 134
Fragments arranged by size:
369 321 233 134 35
BspEI T'CCGG_A
Cuts at: 0 167 827 1092
Size: 167 660 265
Fragments arranged by size:
660 265 167
BspHI T'CATG_A
Cuts at: 0 1077 1092
Size: 1077 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 879 1092
Size: 131 748 213
Fragments arranged by size:
748 213 131
BsrBI CCG'CTC
Cuts at: 0 835 1092
Size: 835 257
BsrDI GCAATG_nn'
Cuts at: 0 452 1092
Size: 452 640
BsrFI r'CCGG_y
Cuts at: 0 78 929 1092
Size: 78 851 163
Fragments arranged by size:
851 163 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 660 1092
Size: 660 432
BtgI C'CryG_G
Cuts at: 0 563 1092
Size: 563 529
DraI TTT'AAA
Cuts at: 0 128 1092
Size: 128 964
DraIII CAC_nnn'GTG
Cuts at: 0 660 1092
Size: 660 432
DrdI GACnn_nn'nnGTC
Cuts at: 0 129 1092
Size: 129 963
EaeI y'GGCC_r
Cuts at: 0 227 583 1065 1092
Size: 227 356 482 27
Fragments arranged by size:
482 356 227 27
EarI CTCTTCn'nnn_
Cuts at: 0 527 686 995 1004 1034 1092
Size: 527 159 309 9 30 58
Fragments arranged by size:
527 309 159 58 30 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 97 634 868 1092
Size: 97 537 234 224
Fragments arranged by size:
537 234 224 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 374 784 904 964 1092
Size: 100 274 410 120 60 128
Fragments arranged by size:
410 274 128 120 100 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 886 1092
Size: 373 513 206
Fragments arranged by size:
513 373 206
EcoO109I rG'GnC_Cy
Cuts at: 0 111 633 634 954 1092
Size: 111 522 1 320 138
Fragments arranged by size:
522 320 138 111 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 291 1092
Size: 291 801
HaeII r_GCGC'y
Cuts at: 0 7 1092
Size: 7 1085
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 273 305 370 402 520 552
Size: 40 32 201 32 65 32 118 32
Cuts at: 552 661 693 776 808 1092
Size: 109 32 83 32 284
Fragments arranged by size:
284 201 118 109 83 65 40 32
32 32 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1092
Size: 533 559
MscI TGG'CCA
Cuts at: 0 585 1092
Size: 585 507
MspA1I CmG'CkG
Cuts at: 0 1070 1092
Size: 1070 22
NaeI GCC'GGC
Cuts at: 0 931 1092
Size: 931 161
NgoMIV G'CCGG_C
Cuts at: 0 929 1092
Size: 929 163
NspI r_CATG'y
Cuts at: 0 457 1092
Size: 457 635
PasI CC'CwG_GG
Cuts at: 0 369 1092
Size: 369 723
PflMI CCAn_nnn'nTGG
Cuts at: 0 435 1092
Size: 435 657
PpuMI rG'GwC_Cy
Cuts at: 0 111 1092
Size: 111 981
PspOMI G'GGCC_C
Cuts at: 0 633 954 1092
Size: 633 321 138
Fragments arranged by size:
633 321 138
SapI GCTCTTCn'nnn_
Cuts at: 0 1034 1092
Size: 1034 58
SexAI A'CCwGG_T
Cuts at: 0 872 1092
Size: 872 220
SmlI C'TyrA_G
Cuts at: 0 973 1092
Size: 973 119
StuI AGG'CCT
Cuts at: 0 706 1015 1092
Size: 706 309 77
Fragments arranged by size:
706 309 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 24 56 1092
Size: 24 32 1036
Fragments arranged by size:
1036 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 874 1092
Size: 874 218
XbaI T'CTAG_A
Cuts at: 0 327 1092
Size: 327 765
Enzymes that do cut:
AcuI AhdI AlfI AlwNI ApaI AvaI BaeI BanI
BanII BbsI BbvCI BciVI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BtgI DraI DraIII DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
MmeI MscI MspA1I NaeI NgoMIV NspI PasI PflMI
PpuMI PspOMI SapI SexAI SmlI StuI TstI Tth111I
XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BcgI BclI BfrBI BglII BlpI
BmgBI BmtI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TatI XcmI XhoI
XmaI XmnI ZraI