Restriction map for
pF1KSDA0865
(Linear) (Six-base) MAPSORT of: KIAA0865.seq Check: 5447 from: 1 to: 5574
KIAA0865.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1552 3506 4510 5574
Size: 1552 1954 1004 1064
Fragments arranged by size:
1954 1552 1064 1004
AccI GT'mk_AC
Cuts at: 0 3333 5574
Size: 3333 2241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 259 568 1532 1660 1835 1944 3076
Size: 163 96 309 964 128 175 109 1132
Cuts at: 3076 3487 3596 5285 5533 5574
Size: 411 109 1689 248 41
Fragments arranged by size:
1689 1132 964 411 309 248 175 163
128 109 109 96 41
AfeI AGC'GCT
Cuts at: 0 1270 5574
Size: 1270 4304
AflIII A'CryG_T
Cuts at: 0 1590 3170 4421 5574
Size: 1590 1580 1251 1153
Fragments arranged by size:
1590 1580 1251 1153
AgeI A'CCGG_T
Cuts at: 0 1856 5574
Size: 1856 3718
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5560 5574
Size: 5560 14
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1380 1412 1969 2001 3221 3253 5574
Size: 1380 32 557 32 1220 32 2321
Fragments arranged by size:
2321 1380 1220 557 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2079 3498 4766 5454 5574
Size: 2079 1419 1268 688 120
Fragments arranged by size:
2079 1419 1268 688 120
ApaI G_GGCC'C
Cuts at: 0 4412 4883 5574
Size: 4412 471 691
Fragments arranged by size:
4412 691 471
ApaLI G'TGCA_C
Cuts at: 0 1850 3099 5574
Size: 1850 1249 2475
Fragments arranged by size:
2475 1850 1249
ApoI r'AATT_y
Cuts at: 0 329 1715 2268 2365 2592 2890 5192 5241
Size: 329 1386 553 97 227 298 2302 49
Cuts at: 5241 5574
Size: 333
Fragments arranged by size:
2302 1386 553 333 329 298 227 97
49
AvaI C'yCGr_G
Cuts at: 0 273 1174 3623 4111 4137 4165 4279 4332
Size: 273 901 2449 488 26 28 114 53
Cuts at: 4332 4738 5040 5574
Size: 406 302 534
Fragments arranged by size:
2449 901 534 488 406 302 273 114
53 28 26
BamHI G'GATC_C
Cuts at: 0 2860 5574
Size: 2860 2714
BanI G'GyrC_C
Cuts at: 0 1555 4132 4197 4836 5475 5574
Size: 1555 2577 65 639 639 99
Fragments arranged by size:
2577 1555 639 639 99 65
BanII G_rGCy'C
Cuts at: 0 986 1235 1944 4345 4412 4609 4796 4883
Size: 986 249 709 2401 67 197 187 87
Cuts at: 4883 5024 5471 5558 5574
Size: 141 447 87 16
Fragments arranged by size:
2401 986 709 447 249 197 187 141
87 87 67 16
BbeI G_GCGC'C
Cuts at: 0 1559 4136 4201 4840 5574
Size: 1559 2577 65 639 734
Fragments arranged by size:
2577 1559 734 639 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 377 5574
Size: 377 5197
BbvCI CC'TCA_GC
Cuts at: 0 5362 5574
Size: 5362 212
BciVI GTATCCnnnnn_n'
Cuts at: 0 3223 5269 5574
Size: 3223 2046 305
Fragments arranged by size:
3223 2046 305
BclI T'GATC_A
Cuts at: 0 756 2822 5574
Size: 756 2066 2752
Fragments arranged by size:
2752 2066 756
BfrBI ATG'CAT
Cuts at: 0 828 1888 5574
Size: 828 1060 3686
Fragments arranged by size:
3686 1060 828
BglI GCCn_nnn'nGGC
Cuts at: 0 1564 3803 4141 4464 4640 4761 4873 4930
Size: 1564 2239 338 323 176 121 112 57
Cuts at: 4930 4945 5574
Size: 15 629
Fragments arranged by size:
2239 1564 629 338 323 176 121 112
57 15
BglII A'GATC_T
Cuts at: 0 1286 3215 5574
Size: 1286 1929 2359
Fragments arranged by size:
2359 1929 1286
BlpI GC'TnA_GC
Cuts at: 0 1225 3179 4075 5465 5574
Size: 1225 1954 896 1390 109
Fragments arranged by size:
1954 1390 1225 896 109
Bme1580I G_kGCm'C
Cuts at: 0 1854 3103 4253 4412 4883 5574
Size: 1854 1249 1150 159 471 691
Fragments arranged by size:
1854 1249 1150 691 471 159
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2067 5574
Size: 2067 3507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 464 653 2056 3223 3328 4561 4585
Size: 445 19 189 1403 1167 105 1233 24
Cuts at: 4585 4938 4977 5430 5574
Size: 353 39 453 144
Fragments arranged by size:
1403 1233 1167 453 445 353 189 144
105 39 24 19
Bpu10I CC'TnA_GC
Cuts at: 0 969 5362 5574
Size: 969 4393 212
Fragments arranged by size:
4393 969 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 4561 5150 5574
Size: 959 3602 589 424
Fragments arranged by size:
3602 959 589 424
BsaI GGTCTCn'nnnn_
Cuts at: 0 582 731 2204 3824 4561 5574
Size: 582 149 1473 1620 737 1013
Fragments arranged by size:
1620 1473 1013 737 582 149
BsaAI yAC'GTr
Cuts at: 0 3171 5574
Size: 3171 2403
BsaHI Gr'CG_yC
Cuts at: 0 382 871 1556 4133 4198 4837 4937 5574
Size: 382 489 685 2577 65 639 100 637
Fragments arranged by size:
2577 685 639 637 489 382 100 65
BsaWI w'CCGG_w
Cuts at: 0 1705 1856 5574
Size: 1705 151 3718
Fragments arranged by size:
3718 1705 151
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 338 368 437 467 626 656 697 727
Size: 338 30 69 30 159 30 41 30
Cuts at: 727 856 886 1405 1435 1969 1999 2600 2630
Size: 129 30 519 30 534 30 601 30
Cuts at: 2630 3023 3053 3326 3356 3739 3769 4276 4291
Size: 393 30 273 30 383 30 507 15
Cuts at: 4291 4306 4321 5574
Size: 15 15 1253
Fragments arranged by size:
1253 601 534 519 507 393 383 338
273 159 129 69 41 30 30 30
30 30 30 30 30 30 30 30
15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 261 359 877 1371 1400 3044 4031 4105
Size: 261 98 518 494 29 1644 987 74
Cuts at: 4105 4312 5061 5427 5574
Size: 207 749 366 147
Fragments arranged by size:
1644 987 749 518 494 366 261 207
147 98 74 29
BseYI C'CCAG_C
Cuts at: 0 1364 3751 4245 4987 5503 5574
Size: 1364 2387 494 742 516 71
Fragments arranged by size:
2387 1364 742 516 494 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3814 4609 5440 5574
Size: 3814 795 831 134
Fragments arranged by size:
3814 831 795 134
BsiEI CG_ry'CG
Cuts at: 0 3776 4468 4695 5574
Size: 3776 692 227 879
Fragments arranged by size:
3776 879 692 227
BsiHKAI G_wGCw'C
Cuts at: 0 303 1235 1854 3103 5024 5558 5574
Size: 303 932 619 1249 1921 534 16
Fragments arranged by size:
1921 1249 932 619 534 303 16
BsmI GAATG_Cn'
Cuts at: 0 3612 5574
Size: 3612 1962
BsmBI CGTCTCn'nnnn_
Cuts at: 0 273 3875 5574
Size: 273 3602 1699
Fragments arranged by size:
3602 1699 273
Bsp1286I G_dGCh'C
Cuts at: 0 303 986 1235 1854 1944 3103 4253 4345
Size: 303 683 249 619 90 1159 1150 92
Cuts at: 4345 4412 4609 4796 4883 5024 5471 5558 5574
Size: 67 197 187 87 141 447 87 16
Fragments arranged by size:
1159 1150 683 619 447 303 249 197
187 141 92 90 87 87 67 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1170 1552 3466 3506 4460 4510 5574
Size: 1170 382 1914 40 954 50 1064
Fragments arranged by size:
1914 1170 1064 954 382 50 40
BsrBI CCG'CTC
Cuts at: 0 1160 5574
Size: 1160 4414
BsrDI GCAATG_nn'
Cuts at: 0 320 392 3117 3617 5574
Size: 320 72 2725 500 1957
Fragments arranged by size:
2725 1957 500 320 72
BsrFI r'CCGG_y
Cuts at: 0 1856 3635 4463 4948 5574
Size: 1856 1779 828 485 626
Fragments arranged by size:
1856 1779 828 626 485
BsrGI T'GTAC_A
Cuts at: 0 2231 2454 4232 5574
Size: 2231 223 1778 1342
Fragments arranged by size:
2231 1778 1342 223
BssHII G'CGCG_C
Cuts at: 0 4150 4181 4385 4769 5574
Size: 4150 31 204 384 805
Fragments arranged by size:
4150 805 384 204 31
BssSI C'ACGA_G
Cuts at: 0 847 2437 5574
Size: 847 1590 3137
Fragments arranged by size:
3137 1590 847
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2488 5454 5574
Size: 2488 2966 120
Fragments arranged by size:
2966 2488 120
BstEII G'GTnAC_C
Cuts at: 0 2494 5574
Size: 2494 3080
BstYI r'GATC_y
Cuts at: 0 1286 2860 3033 3215 4901 5450 5574
Size: 1286 1574 173 182 1686 549 124
Fragments arranged by size:
1686 1574 1286 549 182 173 124
Bsu36I CC'TnA_GG
Cuts at: 0 1383 5574
Size: 1383 4191
BtgI C'CryG_G
Cuts at: 0 1355 2471 2691 3776 3874 3959 4127 4356
Size: 1355 1116 220 1085 98 85 168 229
Cuts at: 4356 4871 5066 5187 5574
Size: 515 195 121 387
Fragments arranged by size:
1355 1116 1085 515 387 229 220 195
168 121 98 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3342 4349 5574
Size: 3342 1007 1225
Fragments arranged by size:
3342 1225 1007
BtsI GCAGTG_nn'
Cuts at: 0 565 3355 4183 5574
Size: 565 2790 828 1391
Fragments arranged by size:
2790 1391 828 565
DraI TTT'AAA
Cuts at: 0 2157 2889 5574
Size: 2157 732 2685
Fragments arranged by size:
2685 2157 732
DraIII CAC_nnn'GTG
Cuts at: 0 849 3837 5574
Size: 849 2988 1737
Fragments arranged by size:
2988 1737 849
DrdI GACnn_nn'nnGTC
Cuts at: 0 346 5574
Size: 346 5228
EaeI y'GGCC_r
Cuts at: 0 39 795 1764 2219 3773 3962 3974 4256
Size: 39 756 969 455 1554 189 12 282
Cuts at: 4256 4465 4692 4874 5574
Size: 209 227 182 700
Fragments arranged by size:
1554 969 756 700 455 282 227 209
189 182 39 12
EagI C'GGCC_G
Cuts at: 0 3773 4465 4692 5574
Size: 3773 692 227 882
Fragments arranged by size:
3773 882 692 227
EarI CTCTTCn'nnn_
Cuts at: 0 974 986 1423 1456 2342 5038 5574
Size: 974 12 437 33 886 2696 536
Fragments arranged by size:
2696 974 886 536 437 33 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2043 4948 5574
Size: 2043 2905 626
Fragments arranged by size:
2905 2043 626
EcoICRI GAG'CTC
Cuts at: 0 1233 5022 5556 5574
Size: 1233 3789 534 18
Fragments arranged by size:
3789 1233 534 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 259 445 464 568 653 1532 1660
Size: 163 96 186 19 104 85 879 128
Cuts at: 1660 1835 1944 2056 3076 3223 3328 3487 3596
Size: 175 109 112 1020 147 105 159 109
Cuts at: 3596 4561 4585 4938 4977 5285 5430 5533 5574
Size: 965 24 353 39 308 145 103 41
Fragments arranged by size:
1020 965 879 353 308 186 175 163
159 147 145 128 112 109 109 105
104 103 96 85 41 39 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 241 460 2041 5538 5574
Size: 241 219 1581 3497 36
Fragments arranged by size:
3497 1581 241 219 36
EcoO109I rG'GnC_Cy
Cuts at: 0 281 3198 3906 4103 4408 4409 4763 4880
Size: 281 2917 708 197 305 1 354 117
Cuts at: 4880 5528 5574
Size: 648 46
Fragments arranged by size:
2917 708 648 354 305 281 197 117
46 1
EcoRI G'AATT_C
Cuts at: 0 2268 5192 5574
Size: 2268 2924 382
Fragments arranged by size:
2924 2268 382
EcoRV GAT'ATC
Cuts at: 0 4097 5574
Size: 4097 1477
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 244 2791 5574
Size: 244 2547 2783
Fragments arranged by size:
2783 2547 244
HaeII r_GCGC'y
Cuts at: 0 1272 1559 3950 4136 4201 4678 4840 5574
Size: 1272 287 2391 186 65 477 162 734
Fragments arranged by size:
2391 1272 734 477 287 186 162 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 338 370 437 469 547 579 626 658
Size: 338 32 67 32 78 32 47 32
Cuts at: 658 697 729 856 888 940 972 1792 1824
Size: 39 32 127 32 52 32 820 32
Cuts at: 1824 1873 1905 3023 3055 3247 3279 3326 3358
Size: 49 32 1118 32 192 32 47 32
Cuts at: 3358 4026 4058 4081 4113 5263 5295 5574
Size: 668 32 23 32 1150 32 279
Fragments arranged by size:
1150 1118 820 668 338 279 192 127
78 67 52 49 47 47 39 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 0 351 357 530 605 1323 2385 3334 5574
Size: 351 6 173 75 718 1062 949 2240
Fragments arranged by size:
2240 1062 949 718 351 173 75 6
HindIII A'AGCT_T
Cuts at: 0 580 5574
Size: 580 4994
HpaI GTT'AAC
Cuts at: 0 605 1323 5574
Size: 605 718 4251
Fragments arranged by size:
4251 718 605
KasI G'GCGC_C
Cuts at: 0 1555 4132 4197 4836 5574
Size: 1555 2577 65 639 738
Fragments arranged by size:
2577 1555 738 639 65
MfeI C'AATT_G
Cuts at: 0 1049 5574
Size: 1049 4525
MluI A'CGCG_T
Cuts at: 0 4421 5574
Size: 4421 1153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 4541 5574
Size: 1052 3489 1033
Fragments arranged by size:
3489 1052 1033
MscI TGG'CCA
Cuts at: 0 41 797 1766 2221 5574
Size: 41 756 969 455 3353
Fragments arranged by size:
3353 969 756 455 41
MslI CAynn'nnrTG
Cuts at: 0 184 212 296 365 2710 4240 5574
Size: 184 28 84 69 2345 1530 1334
Fragments arranged by size:
2345 1530 1334 184 84 69 28
MspA1I CmG'CkG
Cuts at: 0 1368 1464 3319 3961 4079 4129 4611 5068
Size: 1368 96 1855 642 118 50 482 457
Cuts at: 5068 5361 5574
Size: 293 213
Fragments arranged by size:
1855 1368 642 482 457 293 213 118
96 50
NaeI GCC'GGC
Cuts at: 0 4950 5574
Size: 4950 624
NarI GG'CG_CC
Cuts at: 0 1556 4133 4198 4837 5574
Size: 1556 2577 65 639 737
Fragments arranged by size:
2577 1556 737 639 65
NcoI C'CATG_G
Cuts at: 0 1355 2471 5187 5574
Size: 1355 1116 2716 387
Fragments arranged by size:
2716 1355 1116 387
NgoMIV G'CCGG_C
Cuts at: 0 4948 5574
Size: 4948 626
NsiI A_TGCA'T
Cuts at: 0 830 1890 5574
Size: 830 1060 3684
Fragments arranged by size:
3684 1060 830
NspI r_CATG'y
Cuts at: 0 69 151 189 1594 1624 2307 3409 3696
Size: 69 82 38 1405 30 683 1102 287
Cuts at: 3696 5574
Size: 1878
Fragments arranged by size:
1878 1405 1102 683 287 82 69 38
30
PasI CC'CwG_GG
Cuts at: 0 4414 5471 5574
Size: 4414 1057 103
Fragments arranged by size:
4414 1057 103
PciI A'CATG_T
Cuts at: 0 1590 5574
Size: 1590 3984
PflMI CCAn_nnn'nTGG
Cuts at: 0 2079 5574
Size: 2079 3495
PfoI T'CCnGG_A
Cuts at: 0 461 722 1454 2078 3200 4562 5574
Size: 461 261 732 624 1122 1362 1012
Fragments arranged by size:
1362 1122 1012 732 624 461 261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1969 2001 5574
Size: 1969 32 3573
Fragments arranged by size:
3573 1969 32
PpuMI rG'GwC_Cy
Cuts at: 0 281 3198 3906 4103 5574
Size: 281 2917 708 197 1471
Fragments arranged by size:
2917 1471 708 281 197
PshAI GACnn'nnGTC
Cuts at: 0 2904 4495 5574
Size: 2904 1591 1079
Fragments arranged by size:
2904 1591 1079
PsiI TTA'TAA
Cuts at: 0 3454 5574
Size: 3454 2120
PspOMI G'GGCC_C
Cuts at: 0 4408 4879 5574
Size: 4408 471 695
Fragments arranged by size:
4408 695 471
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3646 3678 5574
Size: 3646 32 1896
Fragments arranged by size:
3646 1896 32
PstI C_TGCA'G
Cuts at: 0 1549 3557 4888 5396 5574
Size: 1549 2008 1331 508 178
Fragments arranged by size:
2008 1549 1331 508 178
PvuII CAG'CTG
Cuts at: 0 1368 3319 5361 5574
Size: 1368 1951 2042 213
Fragments arranged by size:
2042 1951 1368 213
SacI G_AGCT'C
Cuts at: 0 1235 5024 5558 5574
Size: 1235 3789 534 16
Fragments arranged by size:
3789 1235 534 16
SacII CC_GC'GG
Cuts at: 0 3962 4130 5069 5574
Size: 3962 168 939 505
Fragments arranged by size:
3962 939 505 168
SalI G'TCGA_C
Cuts at: 0 3332 5574
Size: 3332 2242
SanDI GG'GwC_CC
Cuts at: 0 281 3906 4103 5574
Size: 281 3625 197 1471
Fragments arranged by size:
3625 1471 281 197
SapI GCTCTTCn'nnn_
Cuts at: 0 974 1456 5574
Size: 974 482 4118
Fragments arranged by size:
4118 974 482
SbfI CC_TGCA'GG
Cuts at: 0 1549 5396 5574
Size: 1549 3847 178
Fragments arranged by size:
3847 1549 178
ScaI AGT'ACT
Cuts at: 0 763 4624 5574
Size: 763 3861 950
Fragments arranged by size:
3861 950 763
SfcI C'TryA_G
Cuts at: 0 190 497 1545 3433 3553 4017 4758 4884
Size: 190 307 1048 1888 120 464 741 126
Cuts at: 4884 4977 5392 5574
Size: 93 415 182
Fragments arranged by size:
1888 1048 741 464 415 307 190 182
126 120 93
SfoI GGC'GCC
Cuts at: 0 1557 4134 4199 4838 5574
Size: 1557 2577 65 639 736
Fragments arranged by size:
2577 1557 736 639 65
SmaI CCC'GGG
Cuts at: 0 3625 4113 4139 4167 4281 4334 4740 5574
Size: 3625 488 26 28 114 53 406 834
Fragments arranged by size:
3625 834 488 406 114 53 28 26
SmlI C'TyrA_G
Cuts at: 0 974 4576 5165 5574
Size: 974 3602 589 409
Fragments arranged by size:
3602 974 589 409
SpeI A'CTAG_T
Cuts at: 0 2806 5574
Size: 2806 2768
SphI G_CATG'C
Cuts at: 0 69 151 2307 3409 5574
Size: 69 82 2156 1102 2165
Fragments arranged by size:
2165 2156 1102 82 69
SrfI GCCC'GGGC
Cuts at: 0 4334 4740 5574
Size: 4334 406 834
Fragments arranged by size:
4334 834 406
SspI AAT'ATT
Cuts at: 0 2006 2152 2327 5574
Size: 2006 146 175 3247
Fragments arranged by size:
3247 2006 175 146
StuI AGG'CCT
Cuts at: 0 4998 5574
Size: 4998 576
StyI C'CwwG_G
Cuts at: 0 36 1355 2013 2471 4841 5187 5574
Size: 36 1319 658 458 2370 346 387
Fragments arranged by size:
2370 1319 658 458 387 346 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4865 5574
Size: 4865 709
TatI w'GTAC_w
Cuts at: 0 761 2231 2454 2645 4232 4622 5574
Size: 761 1470 223 191 1587 390 952
Fragments arranged by size:
1587 1470 952 761 390 223 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1405 1437 3739 3771 4291 4323 5574
Size: 1405 32 2302 32 520 32 1251
Fragments arranged by size:
2302 1405 1251 520 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2967 5574
Size: 2967 2607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 469 2533 3799 3984 5574
Size: 469 2064 1266 185 1590
Fragments arranged by size:
2064 1590 1266 469 185
XmaI C'CCGG_G
Cuts at: 0 3623 4111 4137 4165 4279 4332 4738 5574
Size: 3623 488 26 28 114 53 406 836
Fragments arranged by size:
3623 836 488 406 114 53 28 26
XmnI GAAnn'nnTTC
Cuts at: 0 227 1984 2351 3217 5574
Size: 227 1757 367 866 2357
Fragments arranged by size:
2357 1757 866 367 227
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SmaI SmlI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AhdI AleI AscI AseI
AsiSI AvrII BaeI BcgI BmgBI BmrI BmtI BsaBI
BsiWI BspEI BspHI BstBI BstXI BstZ17I ClaI CspCI
FseI FspI FspAI KpnI NdeI NheI NotI NruI
PacI PmeI PmlI PspXI PvuI RsrII SexAI SfiI
SgfI SgrAI SnaBI SwaI Tth111I XhoI ZraI