Restriction map for
pF1KA0864
(Linear) (Six-base) MAPSORT of: KIAA0864.seq Check: 4680 from: 1 to: 3114
KIAA0864.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1947 3114
Size: 1947 1167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 2009 3070 3114
Size: 857 1152 1061 44
Fragments arranged by size:
1152 1061 857 44
AfeI AGC'GCT
Cuts at: 0 1783 3114
Size: 1783 1331
AgeI A'CCGG_T
Cuts at: 0 191 3114
Size: 191 2923
AhdI GACnn_n'nnGTC
Cuts at: 0 1125 3114
Size: 1125 1989
AleI CACnn'nnGTG
Cuts at: 0 313 3114
Size: 313 2801
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2447 2479 3114
Size: 2447 32 635
Fragments arranged by size:
2447 635 32
AlwNI CAG_nnn'CTG
Cuts at: 0 849 1267 1662 1819 2427 2520 2541 2592
Size: 849 418 395 157 608 93 21 51
Cuts at: 2592 2745 3008 3114
Size: 153 263 106
Fragments arranged by size:
849 608 418 395 263 157 153 106
93 51 21
ApaI G_GGCC'C
Cuts at: 0 974 3114
Size: 974 2140
ApaLI G'TGCA_C
Cuts at: 0 187 306 1444 1754 3114
Size: 187 119 1138 310 1360
Fragments arranged by size:
1360 1138 310 187 119
ApoI r'AATT_y
Cuts at: 0 31 1161 3114
Size: 31 1130 1953
Fragments arranged by size:
1953 1130 31
AvaI C'yCGr_G
Cuts at: 0 1524 2035 3114
Size: 1524 511 1079
Fragments arranged by size:
1524 1079 511
AvrII C'CTAG_G
Cuts at: 0 1024 3114
Size: 1024 2090
BamHI G'GATC_C
Cuts at: 0 1421 3114
Size: 1421 1693
BanI G'GyrC_C
Cuts at: 0 286 1959 2417 3114
Size: 286 1673 458 697
Fragments arranged by size:
1673 697 458 286
BanII G_rGCy'C
Cuts at: 0 491 974 1139 1712 1858 2573 2843 3114
Size: 491 483 165 573 146 715 270 271
Fragments arranged by size:
715 573 491 483 271 270 165 146
BbeI G_GCGC'C
Cuts at: 0 2421 3114
Size: 2421 693
BbsI GAAGACnn'nnnn_
Cuts at: 0 820 1976 3114
Size: 820 1156 1138
Fragments arranged by size:
1156 1138 820
BbvCI CC'TCA_GC
Cuts at: 0 1268 2110 3114
Size: 1268 842 1004
Fragments arranged by size:
1268 1004 842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 178 212 1767 1801 3114
Size: 178 34 1555 34 1313
Fragments arranged by size:
1555 1313 178 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1340 3114
Size: 1340 1774
BglI GCCn_nnn'nGGC
Cuts at: 0 338 523 2509 3114
Size: 338 185 1986 605
Fragments arranged by size:
1986 605 338 185
BglII A'GATC_T
Cuts at: 0 2220 3114
Size: 2220 894
BlpI GC'TnA_GC
Cuts at: 0 2842 3114
Size: 2842 272
Bme1580I G_kGCm'C
Cuts at: 0 191 289 310 974 1448 1758 1964 3114
Size: 191 98 21 664 474 310 206 1150
Fragments arranged by size:
1150 664 474 310 206 191 98 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 178 566 1641 2651 2675 2926 3114
Size: 178 388 1075 1010 24 251 188
Fragments arranged by size:
1075 1010 388 251 188 178 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 680 704 1632 2043 2484 2496 2778 3114
Size: 680 24 928 411 441 12 282 336
Fragments arranged by size:
928 680 441 411 336 282 24 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 445 829 1762 2068 2101 2209 2380
Size: 143 302 384 933 306 33 108 171
Cuts at: 2380 2449 2479 2515 2542 3114
Size: 69 30 36 27 572
Fragments arranged by size:
933 572 384 306 302 171 143 108
69 36 33 30 27
Bpu10I CC'TnA_GC
Cuts at: 0 1268 1571 1667 2110 2858 3114
Size: 1268 303 96 443 748 256
Fragments arranged by size:
1268 748 443 303 256 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2752 2881 3114
Size: 2752 129 233
Fragments arranged by size:
2752 233 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 391 949 1510 1924 3114
Size: 200 191 558 561 414 1190
Fragments arranged by size:
1190 561 558 414 200 191
BsaAI yAC'GTr
Cuts at: 0 1443 1899 2154 2168 3114
Size: 1443 456 255 14 946
Fragments arranged by size:
1443 946 456 255 14
BsaHI Gr'CG_yC
Cuts at: 0 367 1073 1147 1799 2418 3114
Size: 367 706 74 652 619 696
Fragments arranged by size:
706 696 652 619 367 74
BsaWI w'CCGG_w
Cuts at: 0 191 1850 2950 3114
Size: 191 1659 1100 164
Fragments arranged by size:
1659 1100 191 164
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 1363 1393 1993 2023 2425 2455
Size: 7 30 1326 30 600 30 402 30
Cuts at: 2455 3114
Size: 659
Fragments arranged by size:
1326 659 600 402 30 30 30 30
7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 751 1052 1285 1596 1783 2302 2446 3114
Size: 751 301 233 311 187 519 144 668
Fragments arranged by size:
751 668 519 311 301 233 187 144
BseYI C'CCAG_C
Cuts at: 0 673 871 877 1056 1554 3114
Size: 673 198 6 179 498 1560
Fragments arranged by size:
1560 673 498 198 179 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 2467 3114
Size: 45 2422 647
Fragments arranged by size:
2422 647 45
BsiEI CG_ry'CG
Cuts at: 0 759 3114
Size: 759 2355
BsiHKAI G_wGCw'C
Cuts at: 0 191 236 310 950 1448 1758 2573 2843
Size: 191 45 74 640 498 310 815 270
Cuts at: 2843 3114
Size: 271
Fragments arranged by size:
815 640 498 310 271 270 191 74
45
BsmI GAATG_Cn'
Cuts at: 0 2505 3114
Size: 2505 609
Bsp1286I G_dGCh'C
Cuts at: 0 191 236 289 310 491 950 974 1139
Size: 191 45 53 21 181 459 24 165
Cuts at: 1139 1448 1712 1758 1858 1964 2573 2843 3114
Size: 309 264 46 100 106 609 270 271
Fragments arranged by size:
609 459 309 271 270 264 191 181
165 106 100 53 46 45 24 21
BspEI T'CCGG_A
Cuts at: 0 2950 3114
Size: 2950 164
BspHI T'CATG_A
Cuts at: 0 1433 3114
Size: 1433 1681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1163 1528 1947 2870 3114
Size: 1163 365 419 923 244
Fragments arranged by size:
1163 923 419 365 244
BsrBI CCG'CTC
Cuts at: 0 794 861 1599 1616 1674 1746 2352 2532
Size: 794 67 738 17 58 72 606 180
Cuts at: 2532 3114
Size: 582
Fragments arranged by size:
794 738 606 582 180 72 67 58
17
BsrDI GCAATG_nn'
Cuts at: 0 2195 3114
Size: 2195 919
BsrFI r'CCGG_y
Cuts at: 0 191 2003 2090 3114
Size: 191 1812 87 1024
Fragments arranged by size:
1812 1024 191 87
BssSI C'ACGA_G
Cuts at: 0 1705 2017 3114
Size: 1705 312 1097
Fragments arranged by size:
1705 1097 312
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2445 2541 3114
Size: 2445 96 573
Fragments arranged by size:
2445 573 96
BstEII G'GTnAC_C
Cuts at: 0 1999 2669 3114
Size: 1999 670 445
Fragments arranged by size:
1999 670 445
BstXI CCAn_nnnn'nTGG
Cuts at: 0 643 966 1116 3114
Size: 643 323 150 1998
Fragments arranged by size:
1998 643 323 150
BstYI r'GATC_y
Cuts at: 0 1421 2220 3004 3114
Size: 1421 799 784 110
Fragments arranged by size:
1421 799 784 110
Bsu36I CC'TnA_GG
Cuts at: 0 281 1721 3114
Size: 281 1440 1393
Fragments arranged by size:
1440 1393 281
BtgI C'CryG_G
Cuts at: 0 1130 2942 3114
Size: 1130 1812 172
Fragments arranged by size:
1812 1130 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1950 2832 3114
Size: 1950 882 282
Fragments arranged by size:
1950 882 282
BtsI GCAGTG_nn'
Cuts at: 0 971 1454 2554 2788 3114
Size: 971 483 1100 234 326
Fragments arranged by size:
1100 971 483 326 234
EaeI y'GGCC_r
Cuts at: 0 771 1200 1391 2297 3114
Size: 771 429 191 906 817
Fragments arranged by size:
906 817 771 429 191
EarI CTCTTCn'nnn_
Cuts at: 0 728 1006 1098 1573 1943 2258 2359 3114
Size: 728 278 92 475 370 315 101 755
Fragments arranged by size:
755 728 475 370 315 278 101 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 409 1428 1741 3114
Size: 409 1019 313 1373
Fragments arranged by size:
1373 1019 409 313
EcoICRI GAG'CTC
Cuts at: 0 2571 2841 3114
Size: 2571 270 273
Fragments arranged by size:
2571 273 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 445 829 857 1762 2009 2068 2101
Size: 143 302 384 28 905 247 59 33
Cuts at: 2101 2209 2380 2449 2479 2515 2542 3070 3114
Size: 108 171 69 30 36 27 528 44
Fragments arranged by size:
905 528 384 302 247 171 143 108
69 59 44 36 33 30 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 280 1524 1666 2284 2680 2749 3114
Size: 118 162 1244 142 618 396 69 365
Fragments arranged by size:
1244 618 396 365 162 142 118 69
EcoO109I rG'GnC_Cy
Cuts at: 0 111 899 970 971 1659 1690 2409 2463
Size: 111 788 71 1 688 31 719 54
Cuts at: 2463 2538 3114
Size: 75 576
Fragments arranged by size:
788 719 688 576 111 75 71 54
31 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1053 3114
Size: 1053 2061
FspI TGC'GCA
Cuts at: 0 1837 3114
Size: 1837 1277
HaeII r_GCGC'y
Cuts at: 0 746 1776 1785 2141 2421 2521 3114
Size: 746 1030 9 356 280 100 593
Fragments arranged by size:
1030 746 593 356 280 100 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 80 264 296 1111 1143 1923 1955
Size: 48 32 184 32 815 32 780 32
Cuts at: 1955 1993 2025 2205 2237 2425 2457 2996 3028
Size: 38 32 180 32 188 32 539 32
Cuts at: 3028 3114
Size: 86
Fragments arranged by size:
815 780 539 188 184 180 86 48
38 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2394 3114
Size: 2394 720
KasI G'GCGC_C
Cuts at: 0 2417 3114
Size: 2417 697
MslI CAynn'nnrTG
Cuts at: 0 295 313 1843 3114
Size: 295 18 1530 1271
Fragments arranged by size:
1530 1271 295 18
MspA1I CmG'CkG
Cuts at: 0 849 1505 1914 2109 2241 2296 2373 2451
Size: 849 656 409 195 132 55 77 78
Cuts at: 2451 3114
Size: 663
Fragments arranged by size:
849 663 656 409 195 132 78 77
55
NarI GG'CG_CC
Cuts at: 0 2418 3114
Size: 2418 696
NruI TCG'CGA
Cuts at: 0 2851 3114
Size: 2851 263
NspI r_CATG'y
Cuts at: 0 1313 3114
Size: 1313 1801
PasI CC'CwG_GG
Cuts at: 0 1858 2121 3114
Size: 1858 263 993
Fragments arranged by size:
1858 993 263
PflMI CCAn_nnn'nTGG
Cuts at: 0 608 2427 2520 3114
Size: 608 1819 93 594
Fragments arranged by size:
1819 608 594 93
PfoI T'CCnGG_A
Cuts at: 0 1943 3114
Size: 1943 1171
PmlI CAC'GTG
Cuts at: 0 1443 1899 2168 3114
Size: 1443 456 269 946
Fragments arranged by size:
1443 946 456 269
PpuMI rG'GwC_Cy
Cuts at: 0 111 899 2463 3114
Size: 111 788 1564 651
Fragments arranged by size:
1564 788 651 111
PspOMI G'GGCC_C
Cuts at: 0 970 3114
Size: 970 2144
PstI C_TGCA'G
Cuts at: 0 2102 2162 2441 2613 2783 3114
Size: 2102 60 279 172 170 331
Fragments arranged by size:
2102 331 279 172 170 60
PvuII CAG'CTG
Cuts at: 0 849 1505 2109 3114
Size: 849 656 604 1005
Fragments arranged by size:
1005 849 656 604
SacI G_AGCT'C
Cuts at: 0 2573 2843 3114
Size: 2573 270 271
Fragments arranged by size:
2573 271 270
SapI GCTCTTCn'nnn_
Cuts at: 0 1098 2359 3114
Size: 1098 1261 755
Fragments arranged by size:
1261 1098 755
SbfI CC_TGCA'GG
Cuts at: 0 2102 3114
Size: 2102 1012
ScaI AGT'ACT
Cuts at: 0 2479 3114
Size: 2479 635
SfcI C'TryA_G
Cuts at: 0 1251 2098 2158 2437 2609 2779 3114
Size: 1251 847 60 279 172 170 335
Fragments arranged by size:
1251 847 335 279 172 170 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 338 3114
Size: 338 2776
SfoI GGC'GCC
Cuts at: 0 2419 3114
Size: 2419 695
SmlI C'TyrA_G
Cuts at: 0 2035 2767 2896 3114
Size: 2035 732 129 218
Fragments arranged by size:
2035 732 218 129
StuI AGG'CCT
Cuts at: 0 925 1530 2068 3059 3114
Size: 925 605 538 991 55
Fragments arranged by size:
991 925 605 538 55
StyI C'CwwG_G
Cuts at: 0 11 401 518 629 926 1024 3114
Size: 11 390 117 111 297 98 2090
Fragments arranged by size:
2090 390 297 117 111 98 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 407 1717 3114
Size: 184 223 1310 1397
Fragments arranged by size:
1397 1310 223 184
TatI w'GTAC_w
Cuts at: 0 2477 2819 3114
Size: 2477 342 295
Fragments arranged by size:
2477 342 295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 261 293 883 915 1189 1221
Size: 7 32 222 32 590 32 274 32
Cuts at: 1221 1314 1346 3114
Size: 93 32 1768
Fragments arranged by size:
1768 590 274 222 93 32 32 32
32 32 7
XhoI C'TCGA_G
Cuts at: 0 2035 3114
Size: 2035 1079
XmnI GAAnn'nnTTC
Cuts at: 0 73 3114
Size: 73 3041
Enzymes that do cut:
AarI AcuI AfeI AgeI AhdI AleI AloI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII KasI MslI MspA1I
NarI NruI NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SapI SbfI ScaI SfcI
SfiI SfoI SmlI StuI StyI TaqII TatI TstI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AlfI AscI
AseI AsiSI BaeI BclI BfrBI BmgBI BmtI BsaBI
BsiWI BsmBI BsrGI BssHII BstBI BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FseI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NsiI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SexAI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XmaI ZraI