Restriction map for
pF1KSDA0862
(Linear) (Six-base) MAPSORT of: KIAA0862.seq Check: 7665 from: 1 to: 1746
KIAA0862.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1550 1746
Size: 539 1011 196
Fragments arranged by size:
1011 539 196
AflII C'TTAA_G
Cuts at: 0 1408 1746
Size: 1408 338
AlwNI CAG_nnn'CTG
Cuts at: 0 235 1746
Size: 235 1511
ApaLI G'TGCA_C
Cuts at: 0 920 1746
Size: 920 826
ApoI r'AATT_y
Cuts at: 0 300 465 548 936 1106 1118 1221 1746
Size: 300 165 83 388 170 12 103 525
Fragments arranged by size:
525 388 300 170 165 103 83 12
BanII G_rGCy'C
Cuts at: 0 281 1746
Size: 281 1465
BciVI GTATCCnnnnn_n'
Cuts at: 0 1046 1746
Size: 1046 700
BglII A'GATC_T
Cuts at: 0 328 1746
Size: 328 1418
BlpI GC'TnA_GC
Cuts at: 0 658 1746
Size: 658 1088
Bme1580I G_kGCm'C
Cuts at: 0 924 1746
Size: 924 822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 153 1110 1567 1746
Size: 113 40 957 457 179
Fragments arranged by size:
957 457 179 113 40
Bpu10I CC'TnA_GC
Cuts at: 0 1011 1746
Size: 1011 735
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1300 1624 1746
Size: 1300 324 122
Fragments arranged by size:
1300 324 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 1273 1746
Size: 1273 473
BseYI C'CCAG_C
Cuts at: 0 228 414 1684 1746
Size: 228 186 1270 62
Fragments arranged by size:
1270 228 186 62
BsiHKAI G_wGCw'C
Cuts at: 0 281 924 1746
Size: 281 643 822
Fragments arranged by size:
822 643 281
BsmI GAATG_Cn'
Cuts at: 0 662 1746
Size: 662 1084
Bsp1286I G_dGCh'C
Cuts at: 0 281 924 1746
Size: 281 643 822
Fragments arranged by size:
822 643 281
BspHI T'CATG_A
Cuts at: 0 443 1634 1746
Size: 443 1191 112
Fragments arranged by size:
1191 443 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 699 1595 1746
Size: 699 896 151
Fragments arranged by size:
896 699 151
BsrDI GCAATG_nn'
Cuts at: 0 264 1746
Size: 264 1482
BsrGI T'GTAC_A
Cuts at: 0 778 1746
Size: 778 968
BstEII G'GTnAC_C
Cuts at: 0 1471 1746
Size: 1471 275
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1330 1746
Size: 1330 416
BstYI r'GATC_y
Cuts at: 0 42 328 1247 1359 1746
Size: 42 286 919 112 387
Fragments arranged by size:
919 387 286 112 42
Bsu36I CC'TnA_GG
Cuts at: 0 1253 1339 1746
Size: 1253 86 407
Fragments arranged by size:
1253 407 86
BtgI C'CryG_G
Cuts at: 0 1323 1739 1746
Size: 1323 416 7
Fragments arranged by size:
1323 416 7
BtsI GCAGTG_nn'
Cuts at: 0 924 1746
Size: 924 822
EaeI y'GGCC_r
Cuts at: 0 220 1746
Size: 220 1526
EarI CTCTTCn'nnn_
Cuts at: 0 290 1361 1460 1746
Size: 290 1071 99 286
Fragments arranged by size:
1071 290 286 99
EcoICRI GAG'CTC
Cuts at: 0 279 1746
Size: 279 1467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 153 539 1110 1550 1567 1746
Size: 113 40 386 571 440 17 179
Fragments arranged by size:
571 440 386 179 113 40 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 1746
Size: 760 986
EcoO109I rG'GnC_Cy
Cuts at: 0 1690 1746
Size: 1690 56
EcoRI G'AATT_C
Cuts at: 0 300 1746
Size: 300 1446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 438 470 1246 1278 1710 1742 1746
Size: 438 32 776 32 432 32 4
Fragments arranged by size:
776 438 432 32 32 32 4
HincII GTy'rAC
Cuts at: 0 204 365 1172 1448 1746
Size: 204 161 807 276 298
Fragments arranged by size:
807 298 276 204 161
HindIII A'AGCT_T
Cuts at: 0 1315 1624 1746
Size: 1315 309 122
Fragments arranged by size:
1315 309 122
HpaI GTT'AAC
Cuts at: 0 1172 1746
Size: 1172 574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 1746
Size: 1337 409
NcoI C'CATG_G
Cuts at: 0 1323 1739 1746
Size: 1323 416 7
Fragments arranged by size:
1323 416 7
NspI r_CATG'y
Cuts at: 0 657 1746
Size: 657 1089
PflMI CCAn_nnn'nTGG
Cuts at: 0 235 1045 1746
Size: 235 810 701
Fragments arranged by size:
810 701 235
PstI C_TGCA'G
Cuts at: 0 1621 1746
Size: 1621 125
SacI G_AGCT'C
Cuts at: 0 281 1746
Size: 281 1465
SfcI C'TryA_G
Cuts at: 0 836 1617 1746
Size: 836 781 129
Fragments arranged by size:
836 781 129
SmlI C'TyrA_G
Cuts at: 0 1279 1408 1603 1746
Size: 1279 129 195 143
Fragments arranged by size:
1279 195 143 129
SphI G_CATG'C
Cuts at: 0 657 1746
Size: 657 1089
StyI C'CwwG_G
Cuts at: 0 62 1184 1323 1500 1739 1746
Size: 62 1122 139 177 239 7
Fragments arranged by size:
1122 239 177 139 62 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1033 1746
Size: 1033 713
TatI w'GTAC_w
Cuts at: 0 778 1746
Size: 778 968
XbaI T'CTAG_A
Cuts at: 0 1362 1746
Size: 1362 384
XmnI GAAnn'nnTTC
Cuts at: 0 755 1746
Size: 755 991
Enzymes that do cut:
AcuI AflII AlwNI ApaLI ApoI BanII BciVI BglII
BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstEII BstXI
BstYI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI Hin4I HincII HindIII HpaI MmeI
NcoI NspI PflMI PstI SacI SfcI SmlI SphI
StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BclI BfrBI BglI BmgBI BmrI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRV FalI FseI FspI FspAI HaeII
KasI KpnI MfeI MluI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TstI Tth111I XcmI XhoI XmaI ZraI