Restriction map for
pF1KSDA0860
(Linear) (Six-base) MAPSORT of: KIAA0860.seq Check: 3568 from: 1 to: 1623
KIAA0860.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1031 1623
Size: 1031 592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1623
Size: 113 1510
AflII C'TTAA_G
Cuts at: 0 516 1623
Size: 516 1107
AflIII A'CryG_T
Cuts at: 0 1110 1623
Size: 1110 513
AleI CACnn'nnGTG
Cuts at: 0 535 1623
Size: 535 1088
AlwNI CAG_nnn'CTG
Cuts at: 0 753 954 1278 1623
Size: 753 201 324 345
Fragments arranged by size:
753 345 324 201
ApaI G_GGCC'C
Cuts at: 0 310 1441 1623
Size: 310 1131 182
Fragments arranged by size:
1131 310 182
ApaLI G'TGCA_C
Cuts at: 0 1569 1623
Size: 1569 54
ApoI r'AATT_y
Cuts at: 0 38 1623
Size: 38 1585
BamHI G'GATC_C
Cuts at: 0 792 1623
Size: 792 831
BanI G'GyrC_C
Cuts at: 0 1405 1623
Size: 1405 218
BanII G_rGCy'C
Cuts at: 0 310 476 492 1226 1441 1623
Size: 310 166 16 734 215 182
Fragments arranged by size:
734 310 215 182 166 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 831 865 1623
Size: 831 34 758
Fragments arranged by size:
831 758 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 562 1623
Size: 562 1061
BglI GCCn_nnn'nGGC
Cuts at: 0 401 1623
Size: 401 1222
BglII A'GATC_T
Cuts at: 0 96 1623
Size: 96 1527
BlpI GC'TnA_GC
Cuts at: 0 314 1623
Size: 314 1309
Bme1580I G_kGCm'C
Cuts at: 0 310 908 1441 1573 1623
Size: 310 598 533 132 50
Fragments arranged by size:
598 533 310 132 50
BmgBI CAC'GTC
Cuts at: 0 1568 1605 1623
Size: 1568 37 18
Fragments arranged by size:
1568 37 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 902 1623
Size: 285 617 721
Fragments arranged by size:
721 617 285
BmtI G_CTAG'C
Cuts at: 0 1598 1623
Size: 1598 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 723 826 886 982 1345 1416 1623
Size: 659 64 103 60 96 363 71 207
Fragments arranged by size:
659 363 207 103 96 71 64 60
Bpu10I CC'TnA_GC
Cuts at: 0 499 1623
Size: 499 1124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1623
Size: 955 668
BsaAI yAC'GTr
Cuts at: 0 507 1623
Size: 507 1116
BsaBI GATnn'nnATC
Cuts at: 0 526 1623
Size: 526 1097
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1480 1510 1623
Size: 1480 30 113
Fragments arranged by size:
1480 113 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 796 1623
Size: 796 827
BseYI C'CCAG_C
Cuts at: 0 214 309 1225 1623
Size: 214 95 916 398
Fragments arranged by size:
916 398 214 95
BsiEI CG_ry'CG
Cuts at: 0 1588 1623
Size: 1588 35
BsiHKAI G_wGCw'C
Cuts at: 0 476 862 1038 1193 1573 1623
Size: 476 386 176 155 380 50
Fragments arranged by size:
476 386 380 176 155 50
Bsp1286I G_dGCh'C
Cuts at: 0 310 476 492 862 908 1038 1193 1226
Size: 310 166 16 370 46 130 155 33
Cuts at: 1226 1441 1573 1623
Size: 215 132 50
Fragments arranged by size:
370 310 215 166 155 132 130 50
46 33 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 607 656 1031 1623
Size: 607 49 375 592
Fragments arranged by size:
607 592 375 49
BsrFI r'CCGG_y
Cuts at: 0 588 1408 1491 1587 1623
Size: 588 820 83 96 36
Fragments arranged by size:
820 588 96 83 36
BsrGI T'GTAC_A
Cuts at: 0 248 1623
Size: 248 1375
BssSI C'ACGA_G
Cuts at: 0 1267 1623
Size: 1267 356
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1577 1623
Size: 1577 46
BstEII G'GTnAC_C
Cuts at: 0 633 1623
Size: 633 990
BstXI CCAn_nnnn'nTGG
Cuts at: 0 804 1027 1369 1623
Size: 804 223 342 254
Fragments arranged by size:
804 342 254 223
BstYI r'GATC_y
Cuts at: 0 96 521 792 1623
Size: 96 425 271 831
Fragments arranged by size:
831 425 271 96
Bsu36I CC'TnA_GG
Cuts at: 0 653 779 837 1623
Size: 653 126 58 786
Fragments arranged by size:
786 653 126 58
BtgI C'CryG_G
Cuts at: 0 686 1623
Size: 686 937
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 445 1623
Size: 445 1178
BtsI GCAGTG_nn'
Cuts at: 0 42 888 1058 1193 1623
Size: 42 846 170 135 430
Fragments arranged by size:
846 430 170 135 42
EaeI y'GGCC_r
Cuts at: 0 590 755 1512 1585 1623
Size: 590 165 757 73 38
Fragments arranged by size:
757 590 165 73 38
EagI C'GGCC_G
Cuts at: 0 1585 1623
Size: 1585 38
EcoICRI GAG'CTC
Cuts at: 0 474 1623
Size: 474 1149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 659 723 826 886 982 1345 1416
Size: 113 546 64 103 60 96 363 71
Cuts at: 1416 1623
Size: 207
Fragments arranged by size:
546 363 207 113 103 96 71 64
60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 652 1623
Size: 652 971
EcoO109I rG'GnC_Cy
Cuts at: 0 416 1438 1623
Size: 416 1022 185
Fragments arranged by size:
1022 416 185
EcoRI G'AATT_C
Cuts at: 0 38 1623
Size: 38 1585
EcoRV GAT'ATC
Cuts at: 0 56 1623
Size: 56 1567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 508 1623
Size: 508 1115
HindIII A'AGCT_T
Cuts at: 0 1287 1623
Size: 1287 336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1623
Size: 551 1072
MslI CAynn'nnrTG
Cuts at: 0 535 1623
Size: 535 1088
MspA1I CmG'CkG
Cuts at: 0 61 214 313 1503 1584 1623
Size: 61 153 99 1190 81 39
Fragments arranged by size:
1190 153 99 81 61 39
NaeI GCC'GGC
Cuts at: 0 590 1623
Size: 590 1033
NcoI C'CATG_G
Cuts at: 0 686 1623
Size: 686 937
NdeI CA'TA_TG
Cuts at: 0 1238 1623
Size: 1238 385
NgoMIV G'CCGG_C
Cuts at: 0 588 1623
Size: 588 1035
NheI G'CTAG_C
Cuts at: 0 1594 1623
Size: 1594 29
NspI r_CATG'y
Cuts at: 0 1114 1623
Size: 1114 509
PasI CC'CwG_GG
Cuts at: 0 303 706 1623
Size: 303 403 917
Fragments arranged by size:
917 403 303
PciI A'CATG_T
Cuts at: 0 1110 1623
Size: 1110 513
PfoI T'CCnGG_A
Cuts at: 0 788 1393 1623
Size: 788 605 230
Fragments arranged by size:
788 605 230
PmlI CAC'GTG
Cuts at: 0 507 1623
Size: 507 1116
PspOMI G'GGCC_C
Cuts at: 0 306 1437 1623
Size: 306 1131 186
Fragments arranged by size:
1131 306 186
PstI C_TGCA'G
Cuts at: 0 671 749 1623
Size: 671 78 874
Fragments arranged by size:
874 671 78
PvuII CAG'CTG
Cuts at: 0 61 214 313 1503 1623
Size: 61 153 99 1190 120
Fragments arranged by size:
1190 153 120 99 61
SacI G_AGCT'C
Cuts at: 0 476 1623
Size: 476 1147
SfcI C'TryA_G
Cuts at: 0 189 667 745 1623
Size: 189 478 78 878
Fragments arranged by size:
878 478 189 78
SmlI C'TyrA_G
Cuts at: 0 516 970 1623
Size: 516 454 653
Fragments arranged by size:
653 516 454
StuI AGG'CCT
Cuts at: 0 1401 1623
Size: 1401 222
StyI C'CwwG_G
Cuts at: 0 347 686 1301 1314 1368 1623
Size: 347 339 615 13 54 255
Fragments arranged by size:
615 347 339 255 54 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 547 1528 1623
Size: 547 981 95
Fragments arranged by size:
981 547 95
TatI w'GTAC_w
Cuts at: 0 248 1623
Size: 248 1375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1164 1623
Size: 1164 459
Enzymes that do cut:
AarI AcuI AflII AflIII AleI AlwNI ApaI ApaLI
ApoI BamHI BanI BanII BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HindIII MmeI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NspI PasI
PciI PfoI PmlI PspOMI PstI PvuII SacI SfcI
SmlI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI
AloI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BbsI BbvCI BclI BfrBI BplI BsaI BsaHI BsaWI
BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BssHII BstBI BstZ17I ClaI CspCI DraI DraIII DrdI
EarI EciI FseI FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MscI NarI NotI
NruI NsiI PacI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI